Restriction map for
pFN21AE1448
(Circular) (Six-base) MAPSORT of: pFN21AE1448.seq Check: 7216 from: 1 to: 5794
pFN21AE1448 5794 bp
With 182 enzymes: SgfI *
August 16, 2010 14:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2020 2020
Size: 5794
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3604 278
Size: 53 83 186 936 2068 2468
Fragments arranged by size:
2468 2068 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3137 1937
Size: 1200 4594
Acc65I G'GTAC_C
Cuts at: 3115 3115
Size: 5794
AclI AA'CG_TT
Cuts at: 1563 3921 4294 1563
Size: 2358 373 3063
Fragments arranged by size:
3063 2358 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2934 3036 3857 5241 5509 1632
Size: 1302 102 821 1384 268 1917
Fragments arranged by size:
1917 1384 1302 821 268 102
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5597
AflIII A'CryG_T
Cuts at: 1203 4693 5670 1203
Size: 3490 977 1327
Fragments arranged by size:
3490 1327 977
AgeI A'CCGG_T
Cuts at: 4625 4625
Size: 5794
AhdI GACnn_n'nnGTC
Cuts at: 4523 4523
Size: 5794
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5794
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5794
AlwNI CAG_nnn'CTG
Cuts at: 2722 3537 5109 2722
Size: 815 1572 3407
Fragments arranged by size:
3407 1572 815
ApaI G_GGCC'C
Cuts at: 2640 2640
Size: 5794
ApaLI G'TGCA_C
Cuts at: 3851 5007 3851
Size: 1156 4638
ApoI r'AATT_y
Cuts at: 1493 2189 3103 3329 3481 5590 1493
Size: 696 914 226 152 2109 1697
Fragments arranged by size:
2109 1697 914 696 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4348 160
Size: 872 3316 1606
Fragments arranged by size:
3316 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5794
AvaI C'yCGr_G
Cuts at: 1870 1943 2023 3119 1870
Size: 73 80 1096 4545
Fragments arranged by size:
4545 1096 80 73
BamHI G'GATC_C
Cuts at: 3124 3491 3124
Size: 367 5427
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2008 3115 4570 618
Size: 325 217 627 221 1107 1455 1842
Fragments arranged by size:
1842 1455 1107 627 325 221 217
BanII G_rGCy'C
Cuts at: 1391 2133 2640 2667 2889 3019 3113 1391
Size: 742 507 27 222 130 94 4072
Fragments arranged by size:
4072 742 507 222 130 94 27
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2660 928
Size: 971 761 4062
Fragments arranged by size:
4062 971 761
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3985 4019 1903
Size: 34 2048 34 3678
Fragments arranged by size:
3678 2048 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3686 4896 3686
Size: 1210 4584
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5794
BfrBI ATG'CAT
Cuts at: 2557 2557
Size: 5794
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2837 4405 136
Size: 107 122 71 631 605 1165 1568 1525
Fragments arranged by size:
1568 1525 1165 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5789 5789
Size: 5794
BlpI GC'TnA_GC
Cuts at: 3211 3211
Size: 5794
Bme1580I G_kGCm'C
Cuts at: 1790 2640 3855 5011 1790
Size: 850 1215 1156 2573
Fragments arranged by size:
2573 1215 1156 850
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5794
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3525 4483 442
Size: 416 53 83 399 24 2108 958 1753
Fragments arranged by size:
2108 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5794
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2075 2123 2124 2810 4454 1239
Size: 836 48 1 686 1644 2579
Fragments arranged by size:
2579 1644 836 686 48 1
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2215 2702 3919 4784 5082 2215
Size: 487 1217 865 298 2927
Fragments arranged by size:
2927 1217 865 487 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4457 882
Size: 617 2958 2219
Fragments arranged by size:
2958 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3585 493
Size: 711 2381 2702
Fragments arranged by size:
2702 2381 711
BsaBI GATnn'nnATC
Cuts at: 5788 5788
Size: 5794
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2020 3601 3983 4618
Size: 53 83 186 936 487 1581 382 635
Cuts at: 4618 275
Size: 1451
Fragments arranged by size:
1581 1451 936 635 487 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4227 4625 4899 5046 5457 4227
Size: 398 274 147 411 4564
Fragments arranged by size:
4564 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5569
Fragments arranged by size:
5569 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2927 1186
Size: 1741 4053
BseYI C'CCAG_C
Cuts at: 2126 2967 4997 5493 2126
Size: 841 2030 496 2427
Fragments arranged by size:
2427 2030 841 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2735 1163
Size: 573 999 4222
Fragments arranged by size:
4222 999 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3244 4005 5033 1540
Size: 275 186 1243 761 1028 2301
Fragments arranged by size:
2301 1243 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2466 2667 3855 3940 5011 2466
Size: 201 1188 85 1071 3249
Fragments arranged by size:
3249 1188 1071 201 85
BsmI GAATG_Cn'
Cuts at: 3305 3398 3305
Size: 93 5701
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2133 2466 2640 2667 2889 3019 3113
Size: 399 343 333 174 27 222 130 94
Cuts at: 3113 3855 3940 5011 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 343 333 222 174
130 94 85 27
BspEI T'CCGG_A
Cuts at: 5457 5457
Size: 5794
BspHI T'CATG_A
Cuts at: 2667 3683 5413 2667
Size: 1016 1730 3048
Fragments arranged by size:
3048 1730 1016
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2020 3149 844
Size: 1176 1129 3489
Fragments arranged by size:
3489 1176 1129
BsrBI CCG'CTC
Cuts at: 1394 1823 3681 1394
Size: 429 1858 3507
Fragments arranged by size:
3507 1858 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4289 4463 65
Size: 1183 3041 174 1396
Fragments arranged by size:
3041 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4438 4625 1700
Size: 110 2628 187 2869
Fragments arranged by size:
2869 2628 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5794
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5794
BssSI C'ACGA_G
Cuts at: 3854 4866 3854
Size: 1012 4782
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5794
BstBI TT'CG_AA
Cuts at: 4598 4598
Size: 5794
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5794
BstXI CCAn_nnnn'nTGG
Cuts at: 2305 2305
Size: 5794
BstYI r'GATC_y
Cuts at: 1972 2340 2764 3124 3491 3876 3893 5345 5431
Size: 368 424 360 367 385 17 1452 86
Cuts at: 5431 5443 5789 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 424 385 368 367 360 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2517 513
Size: 552 939 513 3790
Fragments arranged by size:
3790 939 552 513
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5055
Fragments arranged by size:
5055 683 56
BtsI GCAGTG_nn'
Cuts at: 2154 3310 4104 4124 2154
Size: 1156 794 20 3824
Fragments arranged by size:
3824 1156 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5794
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5759
DraI TTT'AAA
Cuts at: 3098 3098
Size: 5794
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3066 4801 809
Size: 507 1750 1735 1802
Fragments arranged by size:
1802 1750 1735 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3241 4130 5759
Size: 54 1425 50 275 18 1411 889 1629
Cuts at: 5759 8
Size: 43
Fragments arranged by size:
1629 1425 1411 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3241 1537
Size: 275 1429 4090
Fragments arranged by size:
4090 1429 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2102 3724 1353
Size: 749 1622 3423
Fragments arranged by size:
3423 1622 749
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4381 4755 4901 387
Size: 1064 138 2792 374 146 1280
Fragments arranged by size:
2792 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2665 2665
Size: 5794
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2075 2123 2124 2810 2934 3036 3857
Size: 393 443 48 1 686 124 102 821
Cuts at: 3857 4454 5241 5509 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 821 787 686 597 443 393 268
124 102 48 1
EcoRI G'AATT_C
Cuts at: 2189 3103 3481 2189
Size: 914 378 4502
Fragments arranged by size:
4502 914 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3106 3106
Size: 5794
FspI TGC'GCA
Cuts at: 4300 4300
Size: 5794
HaeII r_GCGC'y
Cuts at: 1678 2053 4941 1678
Size: 375 2888 2531
Fragments arranged by size:
2888 2531 375
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2196 2228 4435 4467 4509
Size: 32 135 32 624 32 2207 32 42
Cuts at: 4509 4541 1373
Size: 32 2626
Fragments arranged by size:
2626 2207 624 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2177 3138 3384 669
Size: 988 281 239 961 246 3079
Fragments arranged by size:
3079 988 961 281 246 239
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5794
HpaI GTT'AAC
Cuts at: 3384 3384
Size: 5794
KpnI G_GTAC'C
Cuts at: 3119 3119
Size: 5794
MfeI C'AATT_G
Cuts at: 2904 3393 2904
Size: 489 5305
MluI A'CGCG_T
Cuts at: 5670 5670
Size: 5794
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4908 5092 1305
Size: 3603 184 2007
Fragments arranged by size:
3603 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3972
Fragments arranged by size:
3972 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3752 4111 4270 518
Size: 648 2586 359 159 2042
Fragments arranged by size:
2586 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2967 3210 3887 5035 5280 1606
Size: 1361 243 677 1148 245 2120
Fragments arranged by size:
2120 1361 1148 677 245 243
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4303
Fragments arranged by size:
4303 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5794
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5794
NotI GC'GGCC_GC
Cuts at: 3241 3241
Size: 5794
NsiI A_TGCA'T
Cuts at: 2559 2559
Size: 5794
NspI r_CATG'y
Cuts at: 1132 1229 2557 2989 3152 4697 1132
Size: 97 1328 432 163 1545 2229
Fragments arranged by size:
2229 1545 1328 432 163 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5794
PciI A'CATG_T
Cuts at: 4693 4693
Size: 5794
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2811 3000 1108
Size: 1703 189 3902
Fragments arranged by size:
3902 1703 189
PmeI GTTT'AAAC
Cuts at: 3098 3098
Size: 5794
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3863 3895 5376 5408 3863
Size: 32 1481 32 4249
Fragments arranged by size:
4249 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2791 1613
Size: 1178 4616
PsiI TTA'TAA
Cuts at: 3364 3364
Size: 5794
PspOMI G'GGCC_C
Cuts at: 2636 2636
Size: 5794
PstI C_TGCA'G
Cuts at: 830 3146 830
Size: 2316 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5794
PvuII CAG'CTG
Cuts at: 2967 2967
Size: 5794
SacI G_AGCT'C
Cuts at: 2667 2667
Size: 5794
SalI G'TCGA_C
Cuts at: 1936 3136 1936
Size: 1200 4594
SbfI CC_TGCA'GG
Cuts at: 3146 3146
Size: 5794
ScaI AGT'ACT
Cuts at: 4042 5754 4042
Size: 1712 4082
SfcI C'TryA_G
Cuts at: 826 1045 3142 4277 4958 5149 826
Size: 219 2097 1135 681 191 1471
Fragments arranged by size:
2097 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5794
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5794
SmaI CCC'GGG
Cuts at: 1872 2025 3121 1872
Size: 153 1096 4545
Fragments arranged by size:
4545 1096 153
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2194 2681 3898 4799 5061 820
Size: 197 926 251 487 1217 901 262 1553
Fragments arranged by size:
1553 1217 926 901 487 262 251 197
SnaBI TAC'GTA
Cuts at: 493 3585 493
Size: 3092 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5794
SphI G_CATG'C
Cuts at: 1132 3152 1132
Size: 2020 3774
SspI AAT'ATT
Cuts at: 5 52 3718 5
Size: 47 3666 2081
Fragments arranged by size:
3666 2081 47
StuI AGG'CCT
Cuts at: 1517 2840 1517
Size: 1323 4471
StyI C'CwwG_G
Cuts at: 513 1065 2004 3233 513
Size: 552 939 1229 3074
Fragments arranged by size:
3074 1229 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2175 3821 3838 3991 4176 4621 325
Size: 1255 595 1646 17 153 185 445 1498
Fragments arranged by size:
1646 1498 1255 595 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4040 5752 96
Size: 274 80 33 51 1443 2063 1712 138
Fragments arranged by size:
2063 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2246 2278 5754 5786 2246
Size: 32 3476 32 2254
Fragments arranged by size:
3476 2254 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5794
XbaI T'CTAG_A
Cuts at: 3130 3130
Size: 5794
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5794
XmaI C'CCGG_G
Cuts at: 1870 2023 3119 1870
Size: 153 1096 4545
Fragments arranged by size:
4545 1096 153
XmnI GAAnn'nnTTC
Cuts at: 3923 3923
Size: 5794
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3602 276
Size: 53 83 186 936 2068 2468
Fragments arranged by size:
2468 2068 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BplI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoO109I
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI