Restriction map for
pFN21AE1444
(Circular) (Six-base) MAPSORT of: pFN21AE1444.seq Check: 5179 from: 1 to: 5527
pFN21AE1444 5527 bp
With 182 enzymes: SgfI *
August 16, 2010 14:46 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2054 3337 278
Size: 53 83 186 936 518 1283 2468
Fragments arranged by size:
2468 1283 936 518 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2870 1937
Size: 933 4594
Acc65I G'GTAC_C
Cuts at: 2848 2848
Size: 5527
AclI AA'CG_TT
Cuts at: 1563 3654 4027 1563
Size: 2091 373 3063
Fragments arranged by size:
3063 2091 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2204 3590 4974 5242 1632
Size: 572 1386 1384 268 1917
Fragments arranged by size:
1917 1386 1384 572 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5330
AflIII A'CryG_T
Cuts at: 1203 4426 5403 1203
Size: 3223 977 1327
Fragments arranged by size:
3223 1327 977
AgeI A'CCGG_T
Cuts at: 4358 4358
Size: 5527
AhdI GACnn_n'nnGTC
Cuts at: 4256 4256
Size: 5527
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5527
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5527
AlwNI CAG_nnn'CTG
Cuts at: 3270 4842 3270
Size: 1572 3955
ApaLI G'TGCA_C
Cuts at: 3584 4740 3584
Size: 1156 4371
ApoI r'AATT_y
Cuts at: 1493 2607 2756 2836 3062 3214 5323 1493
Size: 1114 149 80 226 152 2109 1697
Fragments arranged by size:
2109 1697 1114 226 152 149 80
AseI AT'TA_AT
Cuts at: 160 1032 4081 160
Size: 872 3049 1606
Fragments arranged by size:
3049 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5527
AvaI C'yCGr_G
Cuts at: 1870 1943 2852 1870
Size: 73 909 4545
Fragments arranged by size:
4545 909 73
BamHI G'GATC_C
Cuts at: 2484 2857 3224 2484
Size: 373 367 4787
Fragments arranged by size:
4787 373 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2848 4303 618
Size: 325 217 627 1061 1455 1842
Fragments arranged by size:
1842 1455 1061 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2252 2846 1391
Size: 861 594 4072
Fragments arranged by size:
4072 861 594
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4556
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3718 3752 1903
Size: 34 1781 34 3678
Fragments arranged by size:
3678 1781 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2217 3419 4629 2217
Size: 1202 1210 3115
Fragments arranged by size:
3115 1210 1202
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5527
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4138 136
Size: 107 122 71 631 605 2466 1525
Fragments arranged by size:
2466 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5522 5522
Size: 5527
BlpI GC'TnA_GC
Cuts at: 2944 2944
Size: 5527
Bme1580I G_kGCm'C
Cuts at: 1790 2589 3588 4744 1790
Size: 799 999 1156 2573
Fragments arranged by size:
2573 1156 999 799
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5527
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3258 4216 442
Size: 416 53 83 399 24 1841 958 1753
Fragments arranged by size:
1841 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5527
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2577 2660 4187 1239
Size: 1338 83 1527 2579
Fragments arranged by size:
2579 1527 1338 83
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3652 4517 4815 3652
Size: 865 298 4364
Fragments arranged by size:
4364 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4190 882
Size: 617 2691 2219
Fragments arranged by size:
2691 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3318 493
Size: 711 2114 2702
Fragments arranged by size:
2702 2114 711
BsaBI GATnn'nnATC
Cuts at: 5521 5521
Size: 5527
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2051 2601 3334 3716
Size: 53 83 186 936 518 550 733 382
Cuts at: 3716 4351 275
Size: 635 1451
Fragments arranged by size:
1451 936 733 635 550 518 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2418 3960 4358 4632 4779 5190 2418
Size: 1542 398 274 147 411 2755
Fragments arranged by size:
2755 1542 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5302
Fragments arranged by size:
5302 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2756 1186
Size: 1570 3957
BseYI C'CCAG_C
Cuts at: 4730 5226 4730
Size: 496 5031
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4954
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2977 3738 4766 1540
Size: 275 186 976 761 1028 2301
Fragments arranged by size:
2301 1028 976 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2023 3588 3673 4744 2023
Size: 1565 85 1071 2806
Fragments arranged by size:
2806 1565 1071 85
BsmI GAATG_Cn'
Cuts at: 2529 3038 3131 2529
Size: 509 93 4925
Fragments arranged by size:
4925 509 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2023 2252 2589 2846 3588 3673 4744
Size: 399 233 229 337 257 742 85 1071
Cuts at: 4744 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 337 257 233 229
85
BspEI T'CCGG_A
Cuts at: 2418 5190 2418
Size: 2772 2755
BspHI T'CATG_A
Cuts at: 3416 5146 3416
Size: 1730 3797
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2882 844
Size: 2038 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3414 1394
Size: 429 1591 3507
Fragments arranged by size:
3507 1591 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4022 4196 65
Size: 1183 2774 174 1396
Fragments arranged by size:
2774 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4171 4358 1700
Size: 110 2361 187 2869
Fragments arranged by size:
2869 2361 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5527
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5527
BssSI C'ACGA_G
Cuts at: 3587 4599 3587
Size: 1012 4515
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5527
BstBI TT'CG_AA
Cuts at: 4331 4331
Size: 5527
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5527
BstYI r'GATC_y
Cuts at: 1972 2484 2857 3224 3609 3626 5078 5164 5176
Size: 512 373 367 385 17 1452 86 12
Cuts at: 5176 5522 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 512 385 373 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2488 513
Size: 552 939 484 3552
Fragments arranged by size:
3552 939 552 484
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4788
Fragments arranged by size:
4788 683 56
BtsI GCAGTG_nn'
Cuts at: 2324 2439 2464 3043 3837 3857 2324
Size: 115 25 579 794 20 3994
Fragments arranged by size:
3994 794 579 115 25 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5527
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5492
DraI TTT'AAA
Cuts at: 2831 2831
Size: 5527
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2501 4534 809
Size: 507 1185 2033 1802
Fragments arranged by size:
2033 1802 1185 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2513 2974 3863
Size: 54 1425 50 275 18 683 461 889
Cuts at: 3863 5492 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 683 461 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2974 1537
Size: 275 1162 4090
Fragments arranged by size:
4090 1162 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2011 3457 1353
Size: 658 1446 3423
Fragments arranged by size:
3423 1446 658
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4114 4488 4634 387
Size: 1064 138 2525 374 146 1280
Fragments arranged by size:
2525 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2204 2577 2660 3590 4187 4974 5242
Size: 393 572 373 83 930 597 787 268
Cuts at: 5242 1239
Size: 1524
Fragments arranged by size:
1524 930 787 597 572 393 373 268
83
EcoO109I rG'GnC_Cy
Cuts at: 2188 2411 2188
Size: 223 5304
EcoRI G'AATT_C
Cuts at: 2607 2836 3214 2607
Size: 229 378 4920
Fragments arranged by size:
4920 378 229
FspI TGC'GCA
Cuts at: 4033 4033
Size: 5527
HaeII r_GCGC'y
Cuts at: 1678 4674 1678
Size: 2996 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2010 2036 2042 2068 4168
Size: 32 135 32 438 26 6 26 2100
Cuts at: 4168 4200 4242 4274 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2100 438 135 42 32 32 32
32 26 26 6
HincII GTy'rAC
Cuts at: 669 1657 1938 2871 3117 669
Size: 988 281 933 246 3079
Fragments arranged by size:
3079 988 933 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5527
HpaI GTT'AAC
Cuts at: 3117 3117
Size: 5527
KpnI G_GTAC'C
Cuts at: 2852 2852
Size: 5527
MfeI C'AATT_G
Cuts at: 3126 3126
Size: 5527
MluI A'CGCG_T
Cuts at: 5403 5403
Size: 5527
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2103 4641 4825 1305
Size: 798 2538 184 2007
Fragments arranged by size:
2538 2007 798 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2515 10
Size: 54 1425 343 683 3022
Fragments arranged by size:
3022 1425 683 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2541 3485 3844 4003 518
Size: 648 1375 944 359 159 2042
Fragments arranged by size:
2042 1375 944 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2087 2943 3620 4768 5013 1606
Size: 481 856 677 1148 245 2120
Fragments arranged by size:
2120 1148 856 677 481 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4036
Fragments arranged by size:
4036 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5527
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5527
NotI GC'GGCC_GC
Cuts at: 2974 2974
Size: 5527
NspI r_CATG'y
Cuts at: 1132 1229 2885 4430 1132
Size: 97 1656 1545 2229
Fragments arranged by size:
2229 1656 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5527
PciI A'CATG_T
Cuts at: 4426 4426
Size: 5527
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2782 1108
Size: 1674 3853
PfoI T'CCnGG_A
Cuts at: 2616 2616
Size: 5527
PmeI GTTT'AAAC
Cuts at: 2831 2831
Size: 5527
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2348 2380 3596 3628 5109 5141 2348
Size: 32 1216 32 1481 32 2734
Fragments arranged by size:
2734 1481 1216 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2188 2411 2188
Size: 223 5304
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5527
PsiI TTA'TAA
Cuts at: 2232 3097 2232
Size: 865 4662
PstI C_TGCA'G
Cuts at: 830 2086 2319 2879 830
Size: 1256 233 560 3478
Fragments arranged by size:
3478 1256 560 233
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5527
SalI G'TCGA_C
Cuts at: 1936 2869 1936
Size: 933 4594
SapI GCTCTTCn'nnn_
Cuts at: 2011 2011
Size: 5527
SbfI CC_TGCA'GG
Cuts at: 2879 2879
Size: 5527
ScaI AGT'ACT
Cuts at: 3775 5487 3775
Size: 1712 3815
SfcI C'TryA_G
Cuts at: 826 1045 2082 2315 2875 4010 4691 4882 826
Size: 219 1037 233 560 1135 681 191 1471
Fragments arranged by size:
1471 1135 1037 681 560 233 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5527
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5527
SmaI CCC'GGG
Cuts at: 1872 2854 1872
Size: 982 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3631 4532 4794 820
Size: 197 926 1688 901 262 1553
Fragments arranged by size:
1688 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3318 493
Size: 2825 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5527
SphI G_CATG'C
Cuts at: 1132 2885 1132
Size: 1753 3774
SspI AAT'ATT
Cuts at: 5 52 2647 3451 5
Size: 47 2595 804 2081
Fragments arranged by size:
2595 2081 804 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5527
StyI C'CwwG_G
Cuts at: 513 1065 2004 2966 513
Size: 552 939 962 3074
Fragments arranged by size:
3074 962 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3554 3571 3724 3909 4354 325
Size: 1255 1974 17 153 185 445 1498
Fragments arranged by size:
1974 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2469 3773 5485
Size: 274 80 33 51 1443 492 1304 1712
Cuts at: 5485 96
Size: 138
Fragments arranged by size:
1712 1443 1304 492 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2097 2129 5487 5519 2097
Size: 32 3358 32 2105
Fragments arranged by size:
3358 2105 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5527
XbaI T'CTAG_A
Cuts at: 2863 2863
Size: 5527
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5527
XmaI C'CCGG_G
Cuts at: 1870 2852 1870
Size: 982 4545
XmnI GAAnn'nnTTC
Cuts at: 3656 3656
Size: 5527
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2052 3335 276
Size: 53 83 186 936 518 1283 2468
Fragments arranged by size:
2468 1283 936 518 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SexAI SfiI SfoI SrfI SwaI XcmI