Restriction map for
pFN21AE1443
(Circular) (Six-base) MAPSORT of: pFN21AE1443.seq Check: 5881 from: 1 to: 6103
pFN21AE1443 6103 bp
With 182 enzymes: SgfI *
August 16, 2010 14:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3913 278
Size: 53 83 186 936 2377 2468
Fragments arranged by size:
2468 2377 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3069 3446 1937
Size: 1132 377 4594
Fragments arranged by size:
4594 1132 377
Acc65I G'GTAC_C
Cuts at: 3424 3424
Size: 6103
AclI AA'CG_TT
Cuts at: 1563 4230 4603 1563
Size: 2667 373 3063
Fragments arranged by size:
3063 2667 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2768 3130 4166 5550 5818 1632
Size: 1136 362 1036 1384 268 1917
Fragments arranged by size:
1917 1384 1136 1036 362 268
AfeI AGC'GCT
Cuts at: 2138 2138
Size: 6103
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5906
AflIII A'CryG_T
Cuts at: 1203 5002 5979 1203
Size: 3799 977 1327
Fragments arranged by size:
3799 1327 977
AgeI A'CCGG_T
Cuts at: 4934 4934
Size: 6103
AhdI GACnn_n'nnGTC
Cuts at: 4832 4832
Size: 6103
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6103
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6103
AlwNI CAG_nnn'CTG
Cuts at: 3846 5418 3846
Size: 1572 4531
ApaLI G'TGCA_C
Cuts at: 4160 5316 4160
Size: 1156 4947
ApoI r'AATT_y
Cuts at: 1493 3412 3638 3790 5899 1493
Size: 1919 226 152 2109 1697
Fragments arranged by size:
2109 1919 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4657 160
Size: 872 3625 1606
Fragments arranged by size:
3625 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6103
AvaI C'yCGr_G
Cuts at: 1870 1943 2820 2935 2966 3136 3199 3428 1870
Size: 73 877 115 31 170 63 229 4545
Fragments arranged by size:
4545 877 229 170 115 73 63 31
BamHI G'GATC_C
Cuts at: 2378 2881 2917 3433 3800 2378
Size: 503 36 516 367 4681
Fragments arranged by size:
4681 516 503 367 36
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2295 3140 3203 3283 3424
Size: 325 217 627 508 845 63 80 141
Cuts at: 3424 4879 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 845 627 508 325 217 141
80 63
BanII G_rGCy'C
Cuts at: 1391 2063 2524 2967 3028 3422 1391
Size: 672 461 443 61 394 4072
Fragments arranged by size:
4072 672 461 443 394 61
BbeI G_GCGC'C
Cuts at: 3144 3144
Size: 6103
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3111 928
Size: 971 1212 3920
Fragments arranged by size:
3920 1212 971
BbvCI CC'TCA_GC
Cuts at: 2850 2850
Size: 6103
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2687 2721 3012 3046 4294 4328 1903
Size: 34 750 34 291 34 1248 34 3678
Fragments arranged by size:
3678 1248 750 291 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3995 5205 3995
Size: 1210 4893
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6103
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2058 3172 3302
Size: 107 122 71 631 605 386 1114 130
Cuts at: 3302 4714 136
Size: 1412 1525
Fragments arranged by size:
1525 1412 1114 631 605 386 130 122
107 71
BglII A'GATC_T
Cuts at: 2590 2763 6098 2590
Size: 173 3335 2595
Fragments arranged by size:
3335 2595 173
BlpI GC'TnA_GC
Cuts at: 3292 3520 3292
Size: 228 5875
Bme1580I G_kGCm'C
Cuts at: 1790 4164 5320 1790
Size: 2374 1156 2573
Fragments arranged by size:
2573 2374 1156
BmgBI CAC'GTC
Cuts at: 1318 3109 1318
Size: 1791 4312
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3834 4792 442
Size: 416 53 83 399 24 2417 958 1753
Fragments arranged by size:
2417 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3155 1056
Size: 2099 4004
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2068 2978 4763 1239
Size: 829 910 1785 2579
Fragments arranged by size:
2579 1785 910 829
Bpu10I CC'TnA_GC
Cuts at: 2850 3188 2850
Size: 338 5765
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2294 2315 2417 2510 4228 5093 5391 2294
Size: 21 102 93 1718 865 298 3006
Fragments arranged by size:
3006 1718 865 298 102 93 21
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2875 3227 4766 882
Size: 617 1376 352 1539 2219
Fragments arranged by size:
2219 1539 1376 617 352
BsaAI yAC'GTr
Cuts at: 493 1204 3078 3894 493
Size: 711 1874 816 2702
Fragments arranged by size:
2702 1874 816 711
BsaBI GATnn'nnATC
Cuts at: 6097 6097
Size: 6103
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2829 3141 3910 4292
Size: 53 83 186 936 1296 312 769 382
Cuts at: 4292 4927 275
Size: 635 1451
Fragments arranged by size:
1451 1296 936 769 635 382 312 186
83 53
BsaWI w'CCGG_w
Cuts at: 4536 4934 5208 5355 5766 4536
Size: 398 274 147 411 4873
Fragments arranged by size:
4873 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5878
Fragments arranged by size:
5878 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2171 2867 2975 3110 1186
Size: 985 696 108 135 4179
Fragments arranged by size:
4179 985 696 135 108
BseYI C'CCAG_C
Cuts at: 2400 2656 3332 3337 5306 5802 2400
Size: 256 676 5 1969 496 2701
Fragments arranged by size:
2701 1969 676 496 256 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5530
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2207 3553 4314 5342 1540
Size: 275 186 206 1346 761 1028 2301
Fragments arranged by size:
2301 1346 1028 761 275 206 186
BsiHKAI G_wGCw'C
Cuts at: 3083 3194 3213 4164 4249 5320 3083
Size: 111 19 951 85 1071 3866
Fragments arranged by size:
3866 1071 951 111 85 19
BsmI GAATG_Cn'
Cuts at: 3614 3707 3614
Size: 93 6010
BsmBI CGTCTCn'nnnn_
Cuts at: 2836 2836
Size: 6103
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2063 2524 2967 3028 3083 3194 3213
Size: 399 273 461 443 61 55 111 19
Cuts at: 3213 3422 4164 4249 5320 1391
Size: 209 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 461 443 399 273 209
111 85 61 55 19
BspEI T'CCGG_A
Cuts at: 5766 5766
Size: 6103
BspHI T'CATG_A
Cuts at: 3992 5722 3992
Size: 1730 4373
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2447 3458 844
Size: 1603 1011 3489
Fragments arranged by size:
3489 1603 1011
BsrBI CCG'CTC
Cuts at: 1394 1823 3990 1394
Size: 429 2167 3507
Fragments arranged by size:
3507 2167 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4598 4772 65
Size: 1183 3350 174 1396
Fragments arranged by size:
3350 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2039 2174 2292 3377 4747 4934 1700
Size: 110 229 135 118 1085 1370 187 2869
Fragments arranged by size:
2869 1370 1085 229 187 135 118 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6103
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6103
BssSI C'ACGA_G
Cuts at: 4163 5175 4163
Size: 1012 5091
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3380 794
Size: 2586 3517
BstBI TT'CG_AA
Cuts at: 4907 4907
Size: 6103
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6103
BstXI CCAn_nnnn'nTGG
Cuts at: 2668 2734 2794 2815 2668
Size: 66 60 21 5956
Fragments arranged by size:
5956 66 60 21
BstYI r'GATC_y
Cuts at: 1972 2378 2590 2763 2881 2917 3433 3800 4185
Size: 406 212 173 118 36 516 367 385
Cuts at: 4185 4202 5654 5740 5752 6098 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 516 406 385 367 346 212
173 118 86 36 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2802 513
Size: 552 939 798 3814
Fragments arranged by size:
3814 939 798 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5364
Fragments arranged by size:
5364 683 56
BtsI GCAGTG_nn'
Cuts at: 2935 3211 3253 3619 4413 4433 2935
Size: 276 42 366 794 20 4605
Fragments arranged by size:
4605 794 366 276 42 20
ClaI AT'CG_AT
Cuts at: 1554 2697 1554
Size: 1143 4960
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6068
DraI TTT'AAA
Cuts at: 3407 3407
Size: 6103
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5110 809
Size: 507 3794 1802
Fragments arranged by size:
3794 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2037 2204 2214
Size: 54 1425 50 275 18 207 167 10
Cuts at: 2214 2805 3550 4439 6068 8
Size: 591 745 889 1629 43
Fragments arranged by size:
1629 1425 889 745 591 275 207 167
54 50 43 18 10
EagI C'GGCC_G
Cuts at: 1537 1812 2204 3550 1537
Size: 275 392 1346 4090
Fragments arranged by size:
4090 1346 392 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2748 3350 4033 1353
Size: 1395 602 683 3423
Fragments arranged by size:
3423 1395 683 602
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2134 4690 5064 5210 387
Size: 1064 138 545 2556 374 146 1280
Fragments arranged by size:
2556 1280 1064 545 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2068 2768 2978 3130 4166 4763 5550
Size: 393 436 700 210 152 1036 597 787
Cuts at: 5550 5818 1239
Size: 268 1524
Fragments arranged by size:
1524 1036 787 700 597 436 393 268
210 152
EcoNI CCTnn'n_nnAGG
Cuts at: 2216 3089 2216
Size: 873 5230
EcoO109I rG'GnC_Cy
Cuts at: 2362 2362
Size: 6103
EcoRI G'AATT_C
Cuts at: 3412 3790 3412
Size: 378 5725
FspI TGC'GCA
Cuts at: 4609 4609
Size: 6103
HaeII r_GCGC'y
Cuts at: 1678 2140 2175 3144 5250 1678
Size: 462 35 969 2106 2531
Fragments arranged by size:
2531 2106 969 462 35
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4744 4776 4818 4850 1373
Size: 32 135 32 3172 32 42 32 2626
Fragments arranged by size:
3172 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3447 3693 669
Size: 988 281 1509 246 3079
Fragments arranged by size:
3079 1509 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6103
HpaI GTT'AAC
Cuts at: 3693 3693
Size: 6103
KasI G'GCGC_C
Cuts at: 3140 3140
Size: 6103
KpnI G_GTAC'C
Cuts at: 3428 3428
Size: 6103
MfeI C'AATT_G
Cuts at: 3702 3702
Size: 6103
MluI A'CGCG_T
Cuts at: 5979 5979
Size: 6103
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2349 5217 5401 1305
Size: 1044 2868 184 2007
Fragments arranged by size:
2868 2007 1044 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2216 2807 10
Size: 54 1425 343 384 591 3306
Fragments arranged by size:
3306 1425 591 384 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2343 4061 4420 4579 518
Size: 648 1177 1718 359 159 2042
Fragments arranged by size:
2042 1718 1177 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2024 2110 2404 2649 2956 3061 3519 4196
Size: 418 86 294 245 307 105 458 677
Cuts at: 4196 5344 5589 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 458 418 307 294 245
245 105 86
NaeI GCC'GGC
Cuts at: 2041 2176 2294 2041
Size: 135 118 5850
Fragments arranged by size:
5850 135 118
NarI GG'CG_CC
Cuts at: 3141 3141
Size: 6103
NcoI C'CATG_G
Cuts at: 513 1065 2004 2802 513
Size: 552 939 798 3814
Fragments arranged by size:
3814 939 798 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6103
NgoMIV G'CCGG_C
Cuts at: 2039 2174 2292 2039
Size: 135 118 5850
Fragments arranged by size:
5850 135 118
NheI G'CTAG_C
Cuts at: 1052 3151 1052
Size: 2099 4004
NotI GC'GGCC_GC
Cuts at: 2204 3550 2204
Size: 1346 4757
NspI r_CATG'y
Cuts at: 1132 1229 3002 3461 5006 1132
Size: 97 1773 459 1545 2229
Fragments arranged by size:
2229 1773 1545 459 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6103
PciI A'CATG_T
Cuts at: 5002 5002
Size: 6103
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6103
PfoI T'CCnGG_A
Cuts at: 2544 2544
Size: 6103
PmeI GTTT'AAAC
Cuts at: 3407 3407
Size: 6103
PmlI CAC'GTG
Cuts at: 3078 3078
Size: 6103
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4172 4204 5685 5717 4172
Size: 32 1481 32 4558
Fragments arranged by size:
4558 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2362 2362
Size: 6103
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6103
PsiI TTA'TAA
Cuts at: 3673 3673
Size: 6103
PstI C_TGCA'G
Cuts at: 830 2023 2029 2637 2930 3060 3129 3455 830
Size: 1193 6 608 293 130 69 326 3478
Fragments arranged by size:
3478 1193 608 326 293 130 69 6
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6103
PvuII CAG'CTG
Cuts at: 2024 2110 2404 2649 2956 2024
Size: 86 294 245 307 5171
Fragments arranged by size:
5171 307 294 245 86
SalI G'TCGA_C
Cuts at: 1936 3445 1936
Size: 1509 4594
SapI GCTCTTCn'nnn_
Cuts at: 2748 2748
Size: 6103
SbfI CC_TGCA'GG
Cuts at: 3455 3455
Size: 6103
ScaI AGT'ACT
Cuts at: 4351 6063 4351
Size: 1712 4391
SfcI C'TryA_G
Cuts at: 826 1045 2019 2025 2633 2926 3056 3125 3451
Size: 219 974 6 608 293 130 69 326
Cuts at: 3451 4586 5267 5458 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 974 681 608 326 293 219
191 130 69 6
SfoI GGC'GCC
Cuts at: 3142 3142
Size: 6103
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6103
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6103
SmaI CCC'GGG
Cuts at: 1872 2822 2968 3138 3201 3430 1872
Size: 950 146 170 63 229 4545
Fragments arranged by size:
4545 950 229 170 146 63
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2309 2330 2432 2525 4207 5108
Size: 197 926 366 21 102 93 1682 901
Cuts at: 5108 5370 820
Size: 262 1553
Fragments arranged by size:
1682 1553 926 901 366 262 197 102
93 21
SnaBI TAC'GTA
Cuts at: 493 3894 493
Size: 3401 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6103
SphI G_CATG'C
Cuts at: 1132 3002 3461 1132
Size: 1870 459 3774
Fragments arranged by size:
3774 1870 459
SrfI GCCC'GGGC
Cuts at: 2822 2822
Size: 6103
SspI AAT'ATT
Cuts at: 5 52 4027 5
Size: 47 3975 2081
Fragments arranged by size:
3975 2081 47
StuI AGG'CCT
Cuts at: 1517 2386 3186 1517
Size: 869 800 4434
Fragments arranged by size:
4434 869 800
StyI C'CwwG_G
Cuts at: 513 1065 2004 2217 2755 2802 3542 513
Size: 552 939 213 538 47 740 3074
Fragments arranged by size:
3074 939 740 552 538 213 47
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2619 3346 4130 4147 4300 4485 4930
Size: 1255 1039 727 784 17 153 185 445
Cuts at: 4930 325
Size: 1498
Fragments arranged by size:
1498 1255 1039 784 727 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2079 4349 6061
Size: 274 80 33 51 1443 102 2270 1712
Cuts at: 6061 96
Size: 138
Fragments arranged by size:
2270 1712 1443 274 138 102 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3030 3062 6063 6095 3030
Size: 32 3001 32 3038
Fragments arranged by size:
3038 3001 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6103
XbaI T'CTAG_A
Cuts at: 3439 3439
Size: 6103
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6103
XmaI C'CCGG_G
Cuts at: 1870 2820 2966 3136 3199 3428 1870
Size: 950 146 170 63 229 4545
Fragments arranged by size:
4545 950 229 170 146 63
XmnI GAAnn'nnTTC
Cuts at: 4232 4232
Size: 6103
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3911 276
Size: 53 83 186 936 2377 2468
Fragments arranged by size:
2468 2377 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SalI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BfrBI BplI
BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI
FspAI NruI NsiI PacI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SexAI SfiI SwaI XcmI