Restriction map for
pFN21AE1439
(Circular) (Six-base) MAPSORT of: pFN21AE1439.seq Check: 119 from: 1 to: 5092
pFN21AE1439 5092 bp
With 182 enzymes: SgfI *
August 16, 2010 14:46 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2902 278
Size: 53 83 186 936 1366 2468
Fragments arranged by size:
2468 1366 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2435 1937
Size: 498 4594
Acc65I G'GTAC_C
Cuts at: 2413 2413
Size: 5092
AclI AA'CG_TT
Cuts at: 1563 3219 3592 1563
Size: 1656 373 3063
Fragments arranged by size:
3063 1656 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3155 4539 4807 1632
Size: 1523 1384 268 1917
Fragments arranged by size:
1917 1523 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4895
AflIII A'CryG_T
Cuts at: 1203 3991 4968 1203
Size: 2788 977 1327
Fragments arranged by size:
2788 1327 977
AgeI A'CCGG_T
Cuts at: 3923 3923
Size: 5092
AhdI GACnn_n'nnGTC
Cuts at: 2161 3821 2161
Size: 1660 3432
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5092
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5092
AlwNI CAG_nnn'CTG
Cuts at: 2127 2835 4407 2127
Size: 708 1572 2812
Fragments arranged by size:
2812 1572 708
ApaLI G'TGCA_C
Cuts at: 3149 4305 3149
Size: 1156 3936
ApoI r'AATT_y
Cuts at: 1493 2401 2627 2779 4888 1493
Size: 908 226 152 2109 1697
Fragments arranged by size:
2109 1697 908 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3646 160
Size: 872 2614 1606
Fragments arranged by size:
2614 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5092
AvaI C'yCGr_G
Cuts at: 1870 1943 2010 2417 1870
Size: 73 67 407 4545
Fragments arranged by size:
4545 407 73 67
BamHI G'GATC_C
Cuts at: 2422 2789 2422
Size: 367 4725
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2099 2413 3868 618
Size: 325 217 627 312 314 1455 1842
Fragments arranged by size:
1842 1455 627 325 314 312 217
BanII G_rGCy'C
Cuts at: 1391 2018 2055 2411 1391
Size: 627 37 356 4072
Fragments arranged by size:
4072 627 356 37
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4121
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3283 3317 1903
Size: 34 1346 34 3678
Fragments arranged by size:
3678 1346 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2984 4194 2984
Size: 1210 3882
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5092
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3703 136
Size: 107 122 71 631 605 2031 1525
Fragments arranged by size:
2031 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5087 5087
Size: 5092
BlpI GC'TnA_GC
Cuts at: 2509 2509
Size: 5092
Bme1580I G_kGCm'C
Cuts at: 1790 3153 4309 1790
Size: 1363 1156 2573
Fragments arranged by size:
2573 1363 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5092
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2823 3781 442
Size: 416 53 83 399 24 1406 958 1753
Fragments arranged by size:
1753 1406 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5092
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2149 2326 3752 1239
Size: 910 177 1426 2579
Fragments arranged by size:
2579 1426 910 177
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3217 4082 4380 3217
Size: 865 298 3929
Fragments arranged by size:
3929 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2345 3755 882
Size: 617 846 1410 2219
Fragments arranged by size:
2219 1410 846 617
BsaAI yAC'GTr
Cuts at: 493 1204 2883 493
Size: 711 1679 2702
Fragments arranged by size:
2702 1679 711
BsaBI GATnn'nnATC
Cuts at: 5086 5086
Size: 5092
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2899 3281 3916 275
Size: 53 83 186 936 1366 382 635 1451
Fragments arranged by size:
1451 1366 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3525 3923 4197 4344 4755 3525
Size: 398 274 147 411 3862
Fragments arranged by size:
3862 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4867
Fragments arranged by size:
4867 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2030 2363 1186
Size: 844 333 3915
Fragments arranged by size:
3915 844 333
BseYI C'CCAG_C
Cuts at: 2047 2278 2371 4295 4791 2047
Size: 231 93 1924 496 2348
Fragments arranged by size:
2348 1924 496 231 93
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4519
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2180 2542 3303 4331 1540
Size: 275 186 179 362 761 1028 2301
Fragments arranged by size:
2301 1028 761 362 275 186 179
BsiHKAI G_wGCw'C
Cuts at: 3153 3238 4309 3153
Size: 85 1071 3936
Fragments arranged by size:
3936 1071 85
BsmI GAATG_Cn'
Cuts at: 2603 2696 2603
Size: 93 4999
BsmBI CGTCTCn'nnnn_
Cuts at: 2302 2302
Size: 5092
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2018 2055 2411 3153 3238 4309 1391
Size: 399 228 37 356 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 356 228 85 37
BspEI T'CCGG_A
Cuts at: 4755 4755
Size: 5092
BspHI T'CATG_A
Cuts at: 2981 4711 2981
Size: 1730 3362
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2447 844
Size: 1603 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2979 1394
Size: 429 1156 3507
Fragments arranged by size:
3507 1156 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3587 3761 65
Size: 1183 2339 174 1396
Fragments arranged by size:
2339 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2025 2354 3736 3923 1700
Size: 110 215 329 1382 187 2869
Fragments arranged by size:
2869 1382 329 215 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5092
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5092
BssSI C'ACGA_G
Cuts at: 2042 3152 4164 2042
Size: 1110 1012 2970
Fragments arranged by size:
2970 1110 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2157 2219 794
Size: 1363 62 3667
Fragments arranged by size:
3667 1363 62
BstBI TT'CG_AA
Cuts at: 3896 3896
Size: 5092
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5092
BstYI r'GATC_y
Cuts at: 1972 2422 2789 3174 3191 4643 4729 4741 5087
Size: 450 367 385 17 1452 86 12 346
Cuts at: 5087 1972
Size: 1977
Fragments arranged by size:
1977 1452 450 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2076 2116 513
Size: 552 939 72 40 3489
Fragments arranged by size:
3489 939 552 72 40
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4353
Fragments arranged by size:
4353 683 56
BtsI GCAGTG_nn'
Cuts at: 2608 3402 3422 2608
Size: 794 20 4278
Fragments arranged by size:
4278 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5092
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5057
DraI TTT'AAA
Cuts at: 2396 2396
Size: 5092
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4099 809
Size: 507 2783 1802
Fragments arranged by size:
2783 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2079 2539 3428
Size: 54 1425 50 275 18 249 460 889
Cuts at: 3428 5057 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 460 275 249 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2539 1537
Size: 275 727 4090
Fragments arranged by size:
4090 727 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3022 1353
Size: 1669 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2373 3679 4053 4199 387
Size: 1064 138 784 1306 374 146 1280
Fragments arranged by size:
1306 1280 1064 784 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2149 2326 3155 3752 4539 4807 1239
Size: 393 517 177 829 597 787 268 1524
Fragments arranged by size:
1524 829 787 597 517 393 268 177
EcoRI G'AATT_C
Cuts at: 2401 2779 2401
Size: 378 4714
FspI TGC'GCA
Cuts at: 3598 3598
Size: 5092
HaeII r_GCGC'y
Cuts at: 1678 2096 2259 2384 4239 1678
Size: 418 163 125 1855 2531
Fragments arranged by size:
2531 1855 418 163 125
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2147 2179 3733 3765 3807
Size: 32 135 32 575 32 1554 32 42
Cuts at: 3807 3839 1373
Size: 32 2626
Fragments arranged by size:
2626 1554 575 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2436 2682 669
Size: 988 281 498 246 3079
Fragments arranged by size:
3079 988 498 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5092
HpaI GTT'AAC
Cuts at: 2682 2682
Size: 5092
KpnI G_GTAC'C
Cuts at: 2417 2417
Size: 5092
MfeI C'AATT_G
Cuts at: 2691 2691
Size: 5092
MluI A'CGCG_T
Cuts at: 4968 4968
Size: 5092
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4206 4390 1305
Size: 2901 184 2007
Fragments arranged by size:
2901 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3270
Fragments arranged by size:
3270 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3050 3409 3568 518
Size: 648 1884 359 159 2042
Fragments arranged by size:
2042 1884 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2118 2375 2508 3185 4333 4578 1606
Size: 512 257 133 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 512 257 245 133
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3601
Fragments arranged by size:
3601 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5092
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5092
NotI GC'GGCC_GC
Cuts at: 2539 2539
Size: 5092
NspI r_CATG'y
Cuts at: 1132 1229 2450 3995 1132
Size: 97 1221 1545 2229
Fragments arranged by size:
2229 1545 1221 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5092
PciI A'CATG_T
Cuts at: 3991 3991
Size: 5092
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5092
PfoI T'CCnGG_A
Cuts at: 2126 2126
Size: 5092
PmeI GTTT'AAAC
Cuts at: 2396 2396
Size: 5092
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3161 3193 4674 4706 3161
Size: 32 1481 32 3547
Fragments arranged by size:
3547 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5092
PsiI TTA'TAA
Cuts at: 2662 2662
Size: 5092
PstI C_TGCA'G
Cuts at: 830 2212 2444 830
Size: 1382 232 3478
Fragments arranged by size:
3478 1382 232
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5092
PvuII CAG'CTG
Cuts at: 2375 2375
Size: 5092
SacII CC_GC'GG
Cuts at: 2119 2119
Size: 5092
SalI G'TCGA_C
Cuts at: 1936 2434 1936
Size: 498 4594
SbfI CC_TGCA'GG
Cuts at: 2444 2444
Size: 5092
ScaI AGT'ACT
Cuts at: 3340 5052 3340
Size: 1712 3380
SfcI C'TryA_G
Cuts at: 826 1045 2208 2440 3575 4256 4447 826
Size: 219 1163 232 1135 681 191 1471
Fragments arranged by size:
1471 1163 1135 681 232 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5092
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5092
SmaI CCC'GGG
Cuts at: 1872 2419 1872
Size: 547 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3196 4097 4359 820
Size: 197 926 1253 901 262 1553
Fragments arranged by size:
1553 1253 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2883 493
Size: 2390 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5092
SphI G_CATG'C
Cuts at: 1132 2450 1132
Size: 1318 3774
SspI AAT'ATT
Cuts at: 5 52 3016 5
Size: 47 2964 2081
Fragments arranged by size:
2964 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5092
StyI C'CwwG_G
Cuts at: 513 1065 2004 2248 2531 513
Size: 552 939 244 283 3074
Fragments arranged by size:
3074 939 552 283 244
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3119 3136 3289 3474 3919 325
Size: 1255 1539 17 153 185 445 1498
Fragments arranged by size:
1539 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3338 5050 96
Size: 274 80 33 51 1443 1361 1712 138
Fragments arranged by size:
1712 1443 1361 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5052 5084 5052
Size: 32 5060
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5092
XbaI T'CTAG_A
Cuts at: 2428 2428
Size: 5092
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5092
XmaI C'CCGG_G
Cuts at: 1870 2417 1870
Size: 547 4545
XmnI GAAnn'nnTTC
Cuts at: 3221 3221
Size: 5092
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2900 276
Size: 53 83 186 936 1366 2468
Fragments arranged by size:
2468 1366 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PstI PvuI PvuII SacII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PpuMI PspOMI PspXI PsrI RsrII SacI SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI