Restriction map for
pFN21AE1437
(Circular) (Six-base) MAPSORT of: pFN21AE1437.seq Check: 9946 from: 1 to: 7738
pFN21AE1437 7738 bp
With 182 enzymes: SgfI *
August 16, 2010 14:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3101 3101
Size: 7738
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5548 278
Size: 53 83 186 936 4012 2468
Fragments arranged by size:
4012 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3348 3497 5032 5081 1937
Size: 1411 149 1535 49 4594
Fragments arranged by size:
4594 1535 1411 149 49
Acc65I G'GTAC_C
Cuts at: 3176 5059 3176
Size: 1883 5855
AclI AA'CG_TT
Cuts at: 1563 4620 5865 6238 1563
Size: 3057 1245 373 3063
Fragments arranged by size:
3063 3057 1245 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2586 5801 7185 7453 1632
Size: 954 3215 1384 268 1917
Fragments arranged by size:
3215 1917 1384 954 268
AflII C'TTAA_G
Cuts at: 820 1017 3340 820
Size: 197 2323 5218
Fragments arranged by size:
5218 2323 197
AflIII A'CryG_T
Cuts at: 1203 3792 6637 7614 1203
Size: 2589 2845 977 1327
Fragments arranged by size:
2845 2589 1327 977
AgeI A'CCGG_T
Cuts at: 6569 6569
Size: 7738
AhdI GACnn_n'nnGTC
Cuts at: 2292 6467 2292
Size: 4175 3563
AleI CACnn'nnGTG
Cuts at: 1166 4076 4645 1166
Size: 2910 569 4259
Fragments arranged by size:
4259 2910 569
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2423 2907 1184
Size: 1239 484 6015
Fragments arranged by size:
6015 1239 484
AlwNI CAG_nnn'CTG
Cuts at: 3587 5481 7053 3587
Size: 1894 1572 4272
Fragments arranged by size:
4272 1894 1572
ApaI G_GGCC'C
Cuts at: 2975 2975
Size: 7738
ApaLI G'TGCA_C
Cuts at: 5795 6951 5795
Size: 1156 6582
ApoI r'AATT_y
Cuts at: 1493 2141 2628 3525 4470 4541 4585 5047 5273
Size: 648 487 897 945 71 44 462 226
Cuts at: 5273 5425 7534 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 945 897 648 487 462 226
152 71 44
AseI AT'TA_AT
Cuts at: 160 1032 4212 6292 160
Size: 872 3180 2080 1606
Fragments arranged by size:
3180 2080 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7738
AvaI C'yCGr_G
Cuts at: 1870 1943 2652 5063 1870
Size: 73 709 2411 4545
Fragments arranged by size:
4545 2411 709 73
BamHI G'GATC_C
Cuts at: 3369 5068 5435 3369
Size: 1699 367 5672
Fragments arranged by size:
5672 1699 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3176 3919 4060 5059 6514
Size: 325 217 627 1389 743 141 999 1455
Cuts at: 6514 618
Size: 1842
Fragments arranged by size:
1842 1455 1389 999 743 627 325 217
141
BanII G_rGCy'C
Cuts at: 1391 2510 2940 2975 3979 4118 4322 5057 1391
Size: 1119 430 35 1004 139 204 735 4072
Fragments arranged by size:
4072 1119 1004 735 430 204 139 35
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6767
BbvCI CC'TCA_GC
Cuts at: 3486 3486
Size: 7738
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5929 5963 1903
Size: 34 3992 34 3678
Fragments arranged by size:
3992 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5630 6840 5630
Size: 1210 6528
BclI T'GATC_A
Cuts at: 1548 4131 1548
Size: 2583 5155
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6349 136
Size: 107 122 71 631 605 4677 1525
Fragments arranged by size:
4677 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4494 7733 4494
Size: 3239 4499
BlpI GC'TnA_GC
Cuts at: 4514 4746 5155 4514
Size: 232 409 7097
Fragments arranged by size:
7097 409 232
Bme1580I G_kGCm'C
Cuts at: 1790 2975 5799 6955 1790
Size: 1185 2824 1156 2573
Fragments arranged by size:
2824 2573 1185 1156
BmgBI CAC'GTC
Cuts at: 1318 3382 3793 1318
Size: 2064 411 5263
Fragments arranged by size:
5263 2064 411
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5469 6427 442
Size: 416 53 83 399 24 4052 958 1753
Fragments arranged by size:
4052 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7738
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2096 2924 3455 3830 4406 6398 1239
Size: 857 828 531 375 576 1992 2579
Fragments arranged by size:
2579 1992 857 828 576 531 375
Bpu10I CC'TnA_GC
Cuts at: 3486 3486
Size: 7738
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2757 3578 3845 4911 5863 6728 7026 2757
Size: 821 267 1066 952 865 298 3469
Fragments arranged by size:
3469 1066 952 865 821 298 267
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2279 3765 6401 882
Size: 617 780 1486 2636 2219
Fragments arranged by size:
2636 2219 1486 780 617
BsaAI yAC'GTr
Cuts at: 493 1204 2824 5529 493
Size: 711 1620 2705 2702
Fragments arranged by size:
2705 2702 1620 711
BsaBI GATnn'nnATC
Cuts at: 7732 7732
Size: 7738
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2979 4219 5545 5927
Size: 53 83 186 936 1446 1240 1326 382
Cuts at: 5927 6562 275
Size: 635 1451
Fragments arranged by size:
1451 1446 1326 1240 936 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3366 4151 4953 6171 6569 6843 6990 7401 3366
Size: 785 802 1218 398 274 147 411 3703
Fragments arranged by size:
3703 1218 802 785 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3135 3165 4149 4179 1178
Size: 30 165 30 1732 30 984 30 4737
Fragments arranged by size:
4737 1732 984 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2026 2472 2924 2927 2948 3065 3827 4170
Size: 840 446 452 3 21 117 762 343
Cuts at: 4170 4471 4749 1186
Size: 301 278 4175
Fragments arranged by size:
4175 840 762 452 446 343 301 278
117 21 3
BseYI C'CCAG_C
Cuts at: 3445 3636 4111 6941 7437 3445
Size: 191 475 2830 496 3746
Fragments arranged by size:
3746 2830 496 475 191
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3226 4116 1163
Size: 573 1490 890 4785
Fragments arranged by size:
4785 1490 890 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5188 5949 6977 1540
Size: 275 186 3187 761 1028 2301
Fragments arranged by size:
3187 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2659 2838 2886 2940 4173 4322 5799 5884 6955
Size: 179 48 54 1233 149 1477 85 1071
Cuts at: 6955 2659
Size: 3442
Fragments arranged by size:
3442 1477 1233 1071 179 149 85 54
48
BsmI GAATG_Cn'
Cuts at: 5249 5342 5249
Size: 93 7645
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2510 2659 2838 2886 2940 2975 3979
Size: 399 720 149 179 48 54 35 1004
Cuts at: 3979 4118 4173 4322 5057 5799 5884 6955 1391
Size: 139 55 149 735 742 85 1071 2174
Fragments arranged by size:
2174 1071 1004 742 735 720 399 179
149 149 139 85 55 54 48 35
BspEI T'CCGG_A
Cuts at: 4151 4953 7401 4151
Size: 802 2448 4488
Fragments arranged by size:
4488 2448 802
BspHI T'CATG_A
Cuts at: 4807 5627 7357 4807
Size: 820 1730 5188
Fragments arranged by size:
5188 1730 820
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3101 5093 844
Size: 2257 1992 3489
Fragments arranged by size:
3489 2257 1992
BsrBI CCG'CTC
Cuts at: 1394 1823 5625 1394
Size: 429 3802 3507
Fragments arranged by size:
3802 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6233 6407 65
Size: 1183 4985 174 1396
Fragments arranged by size:
4985 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6382 6569 1700
Size: 110 4572 187 2869
Fragments arranged by size:
4572 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7738
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7738
BssSI C'ACGA_G
Cuts at: 2885 3297 5798 6810 2885
Size: 412 2501 1012 3813
Fragments arranged by size:
3813 2501 1012 412
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7738
BstBI TT'CG_AA
Cuts at: 6542 6542
Size: 7738
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7738
BstXI CCAn_nnnn'nTGG
Cuts at: 4606 4606
Size: 7738
BstYI r'GATC_y
Cuts at: 1972 2610 3369 4494 5068 5435 5820 5837 7289
Size: 638 759 1125 574 367 385 17 1452
Cuts at: 7289 7375 7387 7733 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1125 759 638 574 385 367
346 86 17 12
BstZ17I GTA'TAC
Cuts at: 3498 3498
Size: 7738
Bsu36I CC'TnA_GG
Cuts at: 3374 4454 3374
Size: 1080 6658
BtgI C'CryG_G
Cuts at: 513 1065 2004 3997 513
Size: 552 939 1993 4254
Fragments arranged by size:
4254 1993 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3981 487
Size: 683 56 2755 4244
Fragments arranged by size:
4244 2755 683 56
BtsI GCAGTG_nn'
Cuts at: 2407 5254 6048 6068 2407
Size: 2847 794 20 4077
Fragments arranged by size:
4077 2847 794 20
ClaI AT'CG_AT
Cuts at: 1554 2866 1554
Size: 1312 6426
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7703
DraI TTT'AAA
Cuts at: 3570 3594 4423 5042 3570
Size: 24 829 619 6266
Fragments arranged by size:
6266 829 619 24
DraIII CAC_nnn'GTG
Cuts at: 2756 2756
Size: 7738
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3422 6745 809
Size: 507 2106 3323 1802
Fragments arranged by size:
3323 2106 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3013 4068 4875
Size: 54 1425 50 275 18 1183 1055 807
Cuts at: 4875 5185 6074 7703 8
Size: 310 889 1629 43
Fragments arranged by size:
1629 1425 1183 1055 889 807 310 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5185 1537
Size: 275 3373 4090
Fragments arranged by size:
4090 3373 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2574 4370 5668 1353
Size: 1221 1796 1298 3423
Fragments arranged by size:
3423 1796 1298 1221
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3728 6325 6699 6845 387
Size: 1064 138 2139 2597 374 146 1280
Fragments arranged by size:
2597 2139 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2938 4320 2938
Size: 1382 6356
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2096 2586 2924 3455 3830 4406 5801
Size: 393 464 490 338 531 375 576 1395
Cuts at: 5801 6398 7185 7453 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1395 787 597 576 531 490 464
393 375 338 268
EcoNI CCTnn'n_nnAGG
Cuts at: 3681 3782 3681
Size: 101 7637
EcoO109I rG'GnC_Cy
Cuts at: 2596 2972 3442 4126 4725 2596
Size: 376 470 684 599 5609
Fragments arranged by size:
5609 684 599 470 376
EcoRI G'AATT_C
Cuts at: 4585 5047 5425 4585
Size: 462 378 6898
Fragments arranged by size:
6898 462 378
FspI TGC'GCA
Cuts at: 2016 4859 6244 2016
Size: 2843 1385 3510
Fragments arranged by size:
3510 2843 1385
FspAI rTGC'GCAy
Cuts at: 4859 4859
Size: 7738
HaeII r_GCGC'y
Cuts at: 1678 3988 6885 1678
Size: 2310 2897 2531
Fragments arranged by size:
2897 2531 2310
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2278 2310 3779 3811 4789
Size: 32 135 32 706 32 1469 32 978
Cuts at: 4789 4821 6379 6411 6453 6485 1373
Size: 32 1558 32 42 32 2626
Fragments arranged by size:
2626 1558 1469 978 706 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5033 5082 5328 669
Size: 988 281 3095 49 246 3079
Fragments arranged by size:
3095 3079 988 281 246 49
HindIII A'AGCT_T
Cuts at: 748 2130 748
Size: 1382 6356
HpaI GTT'AAC
Cuts at: 5328 5328
Size: 7738
KpnI G_GTAC'C
Cuts at: 3180 5063 3180
Size: 1883 5855
MfeI C'AATT_G
Cuts at: 5337 5337
Size: 7738
MluI A'CGCG_T
Cuts at: 7614 7614
Size: 7738
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3861 4270 6852 7036 1305
Size: 2556 409 2582 184 2007
Fragments arranged by size:
2582 2556 2007 409 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3015 4070 10
Size: 54 1425 343 1183 1055 3678
Fragments arranged by size:
3678 1425 1183 1055 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2301 2889 2990 4058 4076 4604 4645
Size: 648 1135 588 101 1068 18 528 41
Cuts at: 4645 4947 5696 6055 6214 518
Size: 302 749 359 159 2042
Fragments arranged by size:
2042 1135 1068 749 648 588 528 359
302 159 101 41 18
MspA1I CmG'CkG
Cuts at: 1606 3999 5154 5831 6979 7224 1606
Size: 2393 1155 677 1148 245 2120
Fragments arranged by size:
2393 2120 1155 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 6247
Fragments arranged by size:
6247 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7738
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7738
NotI GC'GGCC_GC
Cuts at: 5185 5185
Size: 7738
NspI r_CATG'y
Cuts at: 1132 1229 2788 5096 6641 1132
Size: 97 1559 2308 1545 2229
Fragments arranged by size:
2308 2229 1559 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2323 1348
Size: 975 6763
PciI A'CATG_T
Cuts at: 6637 6637
Size: 7738
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4289 1108
Size: 3181 4557
PfoI T'CCnGG_A
Cuts at: 2765 2940 3807 2765
Size: 175 867 6696
Fragments arranged by size:
6696 867 175
PmeI GTTT'AAAC
Cuts at: 5042 5042
Size: 7738
PmlI CAC'GTG
Cuts at: 2824 2824
Size: 7738
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4746 4778 5807 5839 7320 7352 4746
Size: 32 1029 32 1481 32 5132
Fragments arranged by size:
5132 1481 1029 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2596 3442 2596
Size: 846 6892
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7738
PsiI TTA'TAA
Cuts at: 5308 5308
Size: 7738
PspOMI G'GGCC_C
Cuts at: 2971 2971
Size: 7738
PstI C_TGCA'G
Cuts at: 830 5090 830
Size: 4260 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7738
SacI G_AGCT'C
Cuts at: 2940 4322 2940
Size: 1382 6356
SacII CC_GC'GG
Cuts at: 4000 4000
Size: 7738
SalI G'TCGA_C
Cuts at: 1936 5031 5080 1936
Size: 3095 49 4594
Fragments arranged by size:
4594 3095 49
SbfI CC_TGCA'GG
Cuts at: 5090 5090
Size: 7738
ScaI AGT'ACT
Cuts at: 5986 7698 5986
Size: 1712 6026
SfcI C'TryA_G
Cuts at: 826 1045 3749 4729 4894 5086 6221 6902 7093
Size: 219 2704 980 165 192 1135 681 191
Cuts at: 7093 826
Size: 1471
Fragments arranged by size:
2704 1471 1135 980 681 219 192 191
165
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7738
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7738
SmaI CCC'GGG
Cuts at: 1872 5065 1872
Size: 3193 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2652 2772 3340 3557 3860 4926
Size: 197 926 709 120 568 217 303 1066
Cuts at: 4926 5842 6743 7005 820
Size: 916 901 262 1553
Fragments arranged by size:
1553 1066 926 916 901 709 568 303
262 217 197 120
SnaBI TAC'GTA
Cuts at: 493 5529 493
Size: 5036 2702
SpeI A'CTAG_T
Cuts at: 152 3280 152
Size: 3128 4610
SphI G_CATG'C
Cuts at: 1132 5096 1132
Size: 3964 3774
SspI AAT'ATT
Cuts at: 5 52 2855 5662 5
Size: 47 2803 2807 2081
Fragments arranged by size:
2807 2803 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7738
StyI C'CwwG_G
Cuts at: 513 1065 2004 2592 5177 513
Size: 552 939 588 2585 3074
Fragments arranged by size:
3074 2585 939 588 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2163 5765 5782 5935 6120 6565 325
Size: 1255 583 3602 17 153 185 445 1498
Fragments arranged by size:
3602 1498 1255 583 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5984 7696 96
Size: 274 80 33 51 1443 4007 1712 138
Fragments arranged by size:
4007 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3135 3167 4149 4181 7698 7730 3135
Size: 32 982 32 3517 32 3143
Fragments arranged by size:
3517 3143 982 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7738
XbaI T'CTAG_A
Cuts at: 3541 5074 3541
Size: 1533 6205
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2092 2228 2320 4438 2092
Size: 136 92 2118 5392
Fragments arranged by size:
5392 2118 136 92
XhoI C'TCGA_G
Cuts at: 1943 2652 1943
Size: 709 7029
XmaI C'CCGG_G
Cuts at: 1870 5063 1870
Size: 3193 4545
XmnI GAAnn'nnTTC
Cuts at: 2429 5867 2429
Size: 3438 4300
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5546 276
Size: 53 83 186 936 4012 2468
Fragments arranged by size:
4012 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI SacI SacII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BsmBI EcoRV FalI FseI KasI NaeI NarI
NgoMIV NruI NsiI PacI PspXI PsrI PvuII RsrII
SanDI SapI SexAI SfiI SfoI SrfI SwaI