Restriction map for
pFN21AE1436
(Circular) (Six-base) MAPSORT of: pFN21AE1436.seq Check: 7467 from: 1 to: 5464
pFN21AE1436 5464 bp
With 182 enzymes: SgfI *
August 16, 2010 14:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2495 2495
Size: 5464
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3274 278
Size: 53 83 186 936 1738 2468
Fragments arranged by size:
2468 1738 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2807 1937
Size: 870 4594
Acc65I G'GTAC_C
Cuts at: 2785 2785
Size: 5464
AclI AA'CG_TT
Cuts at: 1563 3591 3964 1563
Size: 2028 373 3063
Fragments arranged by size:
3063 2028 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3527 4911 5179 1632
Size: 1895 1384 268 1917
Fragments arranged by size:
1917 1895 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5267
AflIII A'CryG_T
Cuts at: 1203 4363 5340 1203
Size: 3160 977 1327
Fragments arranged by size:
3160 1327 977
AgeI A'CCGG_T
Cuts at: 2630 4295 2630
Size: 1665 3799
AhdI GACnn_n'nnGTC
Cuts at: 4193 4193
Size: 5464
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5464
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5464
AlwNI CAG_nnn'CTG
Cuts at: 3207 4779 3207
Size: 1572 3892
ApaI G_GGCC'C
Cuts at: 2106 2285 2294 2314 2106
Size: 179 9 20 5256
Fragments arranged by size:
5256 179 20 9
ApaLI G'TGCA_C
Cuts at: 2574 3521 4677 2574
Size: 947 1156 3361
Fragments arranged by size:
3361 1156 947
ApoI r'AATT_y
Cuts at: 1493 2773 2999 3151 5260 1493
Size: 1280 226 152 2109 1697
Fragments arranged by size:
2109 1697 1280 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4018 160
Size: 872 2986 1606
Fragments arranged by size:
2986 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5464
AvaI C'yCGr_G
Cuts at: 1870 1943 2125 2532 2789 1870
Size: 73 182 407 257 4545
Fragments arranged by size:
4545 407 257 182 73
BamHI G'GATC_C
Cuts at: 2794 3161 2794
Size: 367 5097
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2494 2714 2785 4240 618
Size: 325 217 627 707 220 71 1455 1842
Fragments arranged by size:
1842 1455 707 627 325 220 217 71
BanII G_rGCy'C
Cuts at: 1391 2106 2285 2294 2314 2323 2469 2783 1391
Size: 715 179 9 20 9 146 314 4072
Fragments arranged by size:
4072 715 314 179 146 20 9 9
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2662 928
Size: 971 763 3730
Fragments arranged by size:
3730 971 763
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3655 3689 1903
Size: 34 1718 34 3678
Fragments arranged by size:
3678 1718 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2141 3356 4566 2141
Size: 1215 1210 3039
Fragments arranged by size:
3039 1215 1210
BclI T'GATC_A
Cuts at: 1548 2439 1548
Size: 891 4573
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2154 2280 2289
Size: 107 122 71 631 605 482 126 9
Cuts at: 2289 4075 136
Size: 1786 1525
Fragments arranged by size:
1786 1525 631 605 482 126 122 107
71 9
BglII A'GATC_T
Cuts at: 5459 5459
Size: 5464
BlpI GC'TnA_GC
Cuts at: 2090 2881 2090
Size: 791 4673
Bme1580I G_kGCm'C
Cuts at: 1790 2106 2285 2294 2314 2499 2578 3525 4681
Size: 316 179 9 20 185 79 947 1156
Cuts at: 4681 1790
Size: 2573
Fragments arranged by size:
2573 1156 947 316 185 179 79 20
9
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5464
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2030 2711 3195
Size: 416 53 83 399 24 613 681 484
Cuts at: 3195 4153 442
Size: 958 1753
Fragments arranged by size:
1753 958 681 613 484 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5464
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2229 2337 2672 4124 1239
Size: 990 108 335 1452 2579
Fragments arranged by size:
2579 1452 990 335 108
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2630 3589 4454 4752 2630
Size: 959 865 298 3342
Fragments arranged by size:
3342 959 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2205 2232 2507 4127 882
Size: 617 706 27 275 1620 2219
Fragments arranged by size:
2219 1620 706 617 275 27
BsaAI yAC'GTr
Cuts at: 493 1204 2437 3255 493
Size: 711 1233 818 2702
Fragments arranged by size:
2702 1233 818 711
BsaBI GATnn'nnATC
Cuts at: 2015 5458 2015
Size: 3443 2021
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3271 3653 4288 275
Size: 53 83 186 936 1738 382 635 1451
Fragments arranged by size:
1738 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2630 3897 4295 4569 4716 5127 2630
Size: 1267 398 274 147 411 2967
Fragments arranged by size:
2967 1267 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5239
Fragments arranged by size:
5239 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5464
BseYI C'CCAG_C
Cuts at: 2085 2107 4667 5163 2085
Size: 22 2560 496 2386
Fragments arranged by size:
2560 2386 496 22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2080 1163
Size: 573 344 4547
Fragments arranged by size:
4547 573 344
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2914 3675 4703 1540
Size: 275 186 913 761 1028 2301
Fragments arranged by size:
2301 1028 913 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2578 3525 3610 4681 2578
Size: 947 85 1071 3361
Fragments arranged by size:
3361 1071 947 85
BsmI GAATG_Cn'
Cuts at: 2975 3068 2975
Size: 93 5371
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2106 2285 2294 2314 2323 2469 2499
Size: 399 316 179 9 20 9 146 30
Cuts at: 2499 2578 2783 3525 3610 4681 1391
Size: 79 205 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 316 205 179 146
85 79 30 20 9 9
BspEI T'CCGG_A
Cuts at: 5127 5127
Size: 5464
BspHI T'CATG_A
Cuts at: 3353 5083 3353
Size: 1730 3734
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2495 2819 844
Size: 1651 324 3489
Fragments arranged by size:
3489 1651 324
BsrBI CCG'CTC
Cuts at: 1394 1823 3351 1394
Size: 429 1528 3507
Fragments arranged by size:
3507 1528 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3959 4133 65
Size: 1183 2711 174 1396
Fragments arranged by size:
2711 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2630 4108 4295 1700
Size: 110 820 1478 187 2869
Fragments arranged by size:
2869 1478 820 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5464
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5464
BssSI C'ACGA_G
Cuts at: 3524 4536 3524
Size: 1012 4452
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5464
BstBI TT'CG_AA
Cuts at: 4268 4268
Size: 5464
BstEII G'GTnAC_C
Cuts at: 1184 2587 2623 1184
Size: 1403 36 4025
Fragments arranged by size:
4025 1403 36
BstYI r'GATC_y
Cuts at: 1972 2016 2794 3161 3546 3563 5015 5101 5113
Size: 44 778 367 385 17 1452 86 12
Cuts at: 5113 5459 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 778 385 367 346 86 44
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2021 2215 2286 513
Size: 552 956 194 71 3691
Fragments arranged by size:
3691 956 552 194 71
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4725
Fragments arranged by size:
4725 683 56
BtsI GCAGTG_nn'
Cuts at: 2479 2980 3774 3794 2479
Size: 501 794 20 4149
Fragments arranged by size:
4149 794 501 20
ClaI AT'CG_AT
Cuts at: 1554 2070 1554
Size: 516 4948
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5429
DraI TTT'AAA
Cuts at: 2768 2768
Size: 5464
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4471 809
Size: 507 3155 1802
Fragments arranged by size:
3155 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2155 2563 2911
Size: 54 1425 50 275 18 325 408 348
Cuts at: 2911 3800 5429 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 408 348 325 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2911 1537
Size: 275 1099 4090
Fragments arranged by size:
4090 1099 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3394 1353
Size: 2041 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4051 4425 4571 387
Size: 1064 138 2462 374 146 1280
Fragments arranged by size:
2462 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2229 2337 2672 3527 4124 4911 5179
Size: 393 597 108 335 855 597 787 268
Cuts at: 5179 1239
Size: 1524
Fragments arranged by size:
1524 855 787 597 597 393 335 268
108
EcoO109I rG'GnC_Cy
Cuts at: 2103 2166 2281 2282 2291 2310 2311 2327 2103
Size: 63 115 1 9 19 1 16 5240
Fragments arranged by size:
5240 115 63 19 16 9 1 1
EcoRI G'AATT_C
Cuts at: 2773 3151 2773
Size: 378 5086
EcoRV GAT'ATC
Cuts at: 2074 2074
Size: 5464
FspI TGC'GCA
Cuts at: 3970 3970
Size: 5464
HaeII r_GCGC'y
Cuts at: 1678 4611 1678
Size: 2933 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2678 2710 4105 4137 4179
Size: 32 135 32 1106 32 1395 32 42
Cuts at: 4179 4211 1373
Size: 32 2626
Fragments arranged by size:
2626 1395 1106 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2808 3054 669
Size: 988 281 870 246 3079
Fragments arranged by size:
3079 988 870 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5464
HpaI GTT'AAC
Cuts at: 3054 3054
Size: 5464
KpnI G_GTAC'C
Cuts at: 2789 2789
Size: 5464
MfeI C'AATT_G
Cuts at: 3063 3063
Size: 5464
MluI A'CGCG_T
Cuts at: 5340 5340
Size: 5464
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4578 4762 1305
Size: 3273 184 2007
Fragments arranged by size:
3273 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2157 10
Size: 54 1425 343 325 3317
Fragments arranged by size:
3317 1425 343 325 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2447 3422 3781 3940 518
Size: 648 843 438 975 359 159 2042
Fragments arranged by size:
2042 975 843 648 438 359 159
MspA1I CmG'CkG
Cuts at: 1606 2094 2880 3557 4705 4950 1606
Size: 488 786 677 1148 245 2120
Fragments arranged by size:
2120 1148 786 677 488 245
NcoI C'CATG_G
Cuts at: 513 1065 2021 2215 2286 513
Size: 552 956 194 71 3691
Fragments arranged by size:
3691 956 552 194 71
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5464
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5464
NotI GC'GGCC_GC
Cuts at: 2911 2911
Size: 5464
NspI r_CATG'y
Cuts at: 1132 1229 2822 4367 1132
Size: 97 1593 1545 2229
Fragments arranged by size:
2229 1593 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2135 2171 2243 2306 1348
Size: 787 36 72 63 4506
Fragments arranged by size:
4506 787 72 63 36
PciI A'CATG_T
Cuts at: 4363 4363
Size: 5464
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5464
PfoI T'CCnGG_A
Cuts at: 2260 2260
Size: 5464
PmeI GTTT'AAAC
Cuts at: 2768 2768
Size: 5464
PmlI CAC'GTG
Cuts at: 2437 2437
Size: 5464
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3533 3565 5046 5078 3533
Size: 32 1481 32 3919
Fragments arranged by size:
3919 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2166 2166
Size: 5464
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5464
PsiI TTA'TAA
Cuts at: 3034 3034
Size: 5464
PspOMI G'GGCC_C
Cuts at: 2102 2281 2290 2310 2102
Size: 179 9 20 5256
Fragments arranged by size:
5256 179 20 9
PstI C_TGCA'G
Cuts at: 830 2679 2816 830
Size: 1849 137 3478
Fragments arranged by size:
3478 1849 137
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5464
PvuII CAG'CTG
Cuts at: 2094 2094
Size: 5464
SalI G'TCGA_C
Cuts at: 1936 2806 1936
Size: 870 4594
SanDI GG'GwC_CC
Cuts at: 2166 2166
Size: 5464
SbfI CC_TGCA'GG
Cuts at: 2679 2816 2679
Size: 137 5327
ScaI AGT'ACT
Cuts at: 2040 3712 5424 2040
Size: 1672 1712 2080
Fragments arranged by size:
2080 1712 1672
SfcI C'TryA_G
Cuts at: 826 1045 2675 2812 3947 4628 4819 826
Size: 219 1630 137 1135 681 191 1471
Fragments arranged by size:
1630 1471 1135 681 219 191 137
SfiI GGCCn_nnn'nGGCC
Cuts at: 2154 2280 2289 2154
Size: 126 9 5329
Fragments arranged by size:
5329 126 9
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5464
SgrAI Cr'CCGG_yG
Cuts at: 1700 2630 1700
Size: 930 4534
SmaI CCC'GGG
Cuts at: 1872 2127 2791 1872
Size: 255 664 4545
Fragments arranged by size:
4545 664 255
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2532 2645 3568 4469 4731 820
Size: 197 926 589 113 923 901 262 1553
Fragments arranged by size:
1553 926 923 901 589 262 197 113
SnaBI TAC'GTA
Cuts at: 493 3255 493
Size: 2762 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5464
SphI G_CATG'C
Cuts at: 1132 2822 1132
Size: 1690 3774
SspI AAT'ATT
Cuts at: 5 52 3388 5
Size: 47 3336 2081
Fragments arranged by size:
3336 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5464
StyI C'CwwG_G
Cuts at: 513 1065 2021 2215 2277 2286 2903 513
Size: 552 956 194 62 9 617 3074
Fragments arranged by size:
3074 956 617 552 194 62 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2375 3491 3508 3661 3846 4291 325
Size: 1255 795 1116 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1116 795 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2038 3710 5422
Size: 274 80 33 51 1443 61 1672 1712
Cuts at: 5422 96
Size: 138
Fragments arranged by size:
1712 1672 1443 274 138 80 61 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5424 5456 5424
Size: 32 5432
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5464
XbaI T'CTAG_A
Cuts at: 2800 2800
Size: 5464
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2284 2635 2284
Size: 351 5113
XhoI C'TCGA_G
Cuts at: 1943 2532 1943
Size: 589 4875
XmaI C'CCGG_G
Cuts at: 1870 2125 2789 1870
Size: 255 664 4545
Fragments arranged by size:
4545 664 255
XmnI GAAnn'nnTTC
Cuts at: 2669 3593 2669
Size: 924 4540
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3272 276
Size: 53 83 186 936 1738 2468
Fragments arranged by size:
2468 1738 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI
SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PspXI PsrI RsrII SacI
SacII SapI SexAI SfoI SrfI SwaI