Restriction map for
pFN21AE1431
(Circular) (Six-base) MAPSORT of: pFN21AE1431.seq Check: 758 from: 1 to: 6997
pFN21AE1431 6997 bp
With 182 enzymes: SgfI *
August 16, 2010 14:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2444 2444
Size: 6997
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3120 4807 278
Size: 53 83 186 936 1584 1687 2468
Fragments arranged by size:
2468 1687 1584 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3939 4340 1937
Size: 2002 401 4594
Fragments arranged by size:
4594 2002 401
Acc65I G'GTAC_C
Cuts at: 3464 4204 4318 3464
Size: 740 114 6143
Fragments arranged by size:
6143 740 114
AclI AA'CG_TT
Cuts at: 1563 5124 5497 1563
Size: 3561 373 3063
Fragments arranged by size:
3561 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2279 2784 3000 3147 4280 5060 6444 6712
Size: 647 505 216 147 1133 780 1384 268
Cuts at: 6712 1632
Size: 1917
Fragments arranged by size:
1917 1384 1133 780 647 505 268 216
147
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6800
AflIII A'CryG_T
Cuts at: 1203 2184 2222 2615 3355 5896 6873 1203
Size: 981 38 393 740 2541 977 1327
Fragments arranged by size:
2541 1327 981 977 740 393 38
AgeI A'CCGG_T
Cuts at: 5828 5828
Size: 6997
AhdI GACnn_n'nnGTC
Cuts at: 5726 5726
Size: 6997
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6997
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4224 1184
Size: 3040 3957
AlwNI CAG_nnn'CTG
Cuts at: 2176 3427 4740 6312 2176
Size: 1251 1313 1572 2861
Fragments arranged by size:
2861 1572 1313 1251
ApaLI G'TGCA_C
Cuts at: 5054 6210 5054
Size: 1156 5841
ApoI r'AATT_y
Cuts at: 1493 2961 3708 3716 4306 4532 4684 6793 1493
Size: 1468 747 8 590 226 152 2109 1697
Fragments arranged by size:
2109 1697 1468 747 590 226 152 8
AseI AT'TA_AT
Cuts at: 160 1032 3687 5551 160
Size: 872 2655 1864 1606
Fragments arranged by size:
2655 1864 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6997
AvaI C'yCGr_G
Cuts at: 1870 1943 2506 3816 4218 4322 1870
Size: 73 563 1310 402 104 4545
Fragments arranged by size:
4545 1310 563 402 104 73
BamHI G'GATC_C
Cuts at: 2108 4327 4694 2108
Size: 2219 367 4411
Fragments arranged by size:
4411 2219 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3464 4204 4318 5773 618
Size: 325 217 627 1677 740 114 1455 1842
Fragments arranged by size:
1842 1677 1455 740 627 325 217 114
BanII G_rGCy'C
Cuts at: 1391 2423 4316 1391
Size: 1032 1893 4072
Fragments arranged by size:
4072 1893 1032
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2267 2562 2703 3045 928
Size: 971 368 295 141 342 4880
Fragments arranged by size:
4880 971 368 342 295 141
BbvCI CC'TCA_GC
Cuts at: 2745 2745
Size: 6997
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5188 5222 1903
Size: 34 3251 34 3678
Fragments arranged by size:
3678 3251 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4889 6099 4889
Size: 1210 5787
BclI T'GATC_A
Cuts at: 1548 3741 1548
Size: 2193 4804
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3083 5608 136
Size: 107 122 71 631 605 1411 2525 1525
Fragments arranged by size:
2525 1525 1411 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2234 2382 3498 6992 2234
Size: 148 1116 3494 2239
Fragments arranged by size:
3494 2239 1116 148
BlpI GC'TnA_GC
Cuts at: 4414 4414
Size: 6997
Bme1580I G_kGCm'C
Cuts at: 1790 2757 5058 6214 1790
Size: 967 2301 1156 2573
Fragments arranged by size:
2573 2301 1156 967
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6997
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3013 4728 5686
Size: 416 53 83 399 24 1596 1715 958
Cuts at: 5686 442
Size: 1753
Fragments arranged by size:
1753 1715 1596 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6997
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3849 3999 3849
Size: 150 6847
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2085 2141 2198 2269 3065 3668 4074 5657
Size: 846 56 57 71 796 603 406 1583
Cuts at: 5657 1239
Size: 2579
Fragments arranged by size:
2579 1583 846 796 603 406 71 57
56
Bpu10I CC'TnA_GC
Cuts at: 2745 2745
Size: 6997
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2250 2670 3734 5122 5987 6285 2250
Size: 420 1064 1388 865 298 2962
Fragments arranged by size:
2962 1388 1064 865 420 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2361 2717 5660 882
Size: 617 862 356 2943 2219
Fragments arranged by size:
2943 2219 862 617 356
BsaAI yAC'GTr
Cuts at: 493 1204 2185 2225 4788 493
Size: 711 981 40 2563 2702
Fragments arranged by size:
2702 2563 981 711 40
BsaBI GATnn'nnATC
Cuts at: 6991 6991
Size: 6997
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3117 4804 5186 5821
Size: 53 83 186 936 1584 1687 382 635
Cuts at: 5821 275
Size: 1451
Fragments arranged by size:
1687 1584 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5430 5828 6102 6249 6660 5430
Size: 398 274 147 411 5767
Fragments arranged by size:
5767 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2065 2095 2171 2201 1178
Size: 30 165 30 662 30 76 30 5974
Fragments arranged by size:
5974 662 165 76 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3923 3926 4071 1186
Size: 2737 3 145 4112
Fragments arranged by size:
4112 2737 145 3
BseYI C'CCAG_C
Cuts at: 2604 4158 6200 6696 2604
Size: 1554 2042 496 2905
Fragments arranged by size:
2905 2042 1554 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2237 2317 3784 3861 1163
Size: 573 501 80 1467 77 4299
Fragments arranged by size:
4299 1467 573 501 80 77
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4447 5208 6236 1540
Size: 275 186 2446 761 1028 2301
Fragments arranged by size:
2446 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2255 5058 5143 6214 2255
Size: 2803 85 1071 3038
Fragments arranged by size:
3038 2803 1071 85
BsmI GAATG_Cn'
Cuts at: 4508 4601 4508
Size: 93 6904
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2255 2423 2757 4316 5058 5143 6214
Size: 399 465 168 334 1559 742 85 1071
Cuts at: 6214 1391
Size: 2174
Fragments arranged by size:
2174 1559 1071 742 465 399 334 168
85
BspEI T'CCGG_A
Cuts at: 6660 6660
Size: 6997
BspHI T'CATG_A
Cuts at: 3162 4886 6616 3162
Size: 1724 1730 3543
Fragments arranged by size:
3543 1730 1724
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2237 2444 4192 4216 4352 844
Size: 1393 207 1748 24 136 3489
Fragments arranged by size:
3489 1748 1393 207 136 24
BsrBI CCG'CTC
Cuts at: 1394 1823 2489 2640 4884 1394
Size: 429 666 151 2244 3507
Fragments arranged by size:
3507 2244 666 429 151
BsrDI GCAATG_nn'
Cuts at: 65 1248 5492 5666 65
Size: 1183 4244 174 1396
Fragments arranged by size:
4244 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2451 5641 5828 1700
Size: 110 641 3190 187 2869
Fragments arranged by size:
3190 2869 641 187 110
BsrGI T'GTAC_A
Cuts at: 96 3090 96
Size: 2994 4003
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6997
BssSI C'ACGA_G
Cuts at: 3527 5057 6069 3527
Size: 1530 1012 4455
Fragments arranged by size:
4455 1530 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6997
BstBI TT'CG_AA
Cuts at: 5801 5801
Size: 6997
BstEII G'GTnAC_C
Cuts at: 1184 4030 1184
Size: 2846 4151
BstXI CCAn_nnnn'nTGG
Cuts at: 2447 2447
Size: 6997
BstYI r'GATC_y
Cuts at: 1972 2108 2234 2382 3216 3498 4327 4694 5079
Size: 136 126 148 834 282 829 367 385
Cuts at: 5079 5096 6548 6634 6646 6992 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 834 829 385 367 346 282
148 136 126 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2084 3485 513
Size: 552 939 80 1401 4025
Fragments arranged by size:
4025 1401 939 552 80
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6258
Fragments arranged by size:
6258 683 56
BtsI GCAGTG_nn'
Cuts at: 2392 2572 3093 4513 5307 5327 2392
Size: 180 521 1420 794 20 4062
Fragments arranged by size:
4062 1420 794 521 180 20
ClaI AT'CG_AT
Cuts at: 1554 3411 1554
Size: 1857 5140
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2514 2549 529
Size: 35 1950 35 4977
Fragments arranged by size:
4977 1950 35 35
DraI TTT'AAA
Cuts at: 4301 4301
Size: 6997
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6004 809
Size: 507 4688 1802
Fragments arranged by size:
4688 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2449 2694 3107
Size: 54 1425 50 275 18 619 245 413
Cuts at: 3107 3769 4444 5333 6962 8
Size: 662 675 889 1629 43
Fragments arranged by size:
1629 1425 889 675 662 619 413 275
245 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4444 1537
Size: 275 2632 4090
Fragments arranged by size:
4090 2632 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3858 4927 1353
Size: 2505 1069 3423
Fragments arranged by size:
3423 2505 1069
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5584 5958 6104 387
Size: 1064 138 3995 374 146 1280
Fragments arranged by size:
3995 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2085 2141 2198 2269 2279 2784 3000
Size: 393 453 56 57 71 10 505 216
Cuts at: 3000 3065 3147 3668 4074 4280 5060 5657 6444
Size: 65 82 521 406 206 780 597 787
Cuts at: 6444 6712 1239
Size: 268 1524
Fragments arranged by size:
1524 787 780 597 521 505 453 406
393 268 216 206 82 71 65 57
56 10
EcoNI CCTnn'n_nnAGG
Cuts at: 3126 3126
Size: 6997
EcoO109I rG'GnC_Cy
Cuts at: 2244 3076 3193 3643 2244
Size: 832 117 450 5598
Fragments arranged by size:
5598 832 450 117
EcoRI G'AATT_C
Cuts at: 3708 4306 4684 3708
Size: 598 378 6021
Fragments arranged by size:
6021 598 378
EcoRV GAT'ATC
Cuts at: 3886 3886
Size: 6997
FspI TGC'GCA
Cuts at: 5503 5503
Size: 6997
HaeII r_GCGC'y
Cuts at: 1678 6144 1678
Size: 4466 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2065 2097 2369 2401 5638
Size: 32 135 32 493 32 272 32 3237
Cuts at: 5638 5670 5712 5744 1373
Size: 32 42 32 2626
Fragments arranged by size:
3237 2626 493 272 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2887 4341 4587 669
Size: 988 281 949 1454 246 3079
Fragments arranged by size:
3079 1454 988 949 281 246
HindIII A'AGCT_T
Cuts at: 748 2700 748
Size: 1952 5045
HpaI GTT'AAC
Cuts at: 4587 4587
Size: 6997
KpnI G_GTAC'C
Cuts at: 3468 4208 4322 3468
Size: 740 114 6143
Fragments arranged by size:
6143 740 114
MfeI C'AATT_G
Cuts at: 4596 4596
Size: 6997
MluI A'CGCG_T
Cuts at: 6873 6873
Size: 6997
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2176 2526 3860 6111 6295 1305
Size: 871 350 1334 2251 184 2007
Fragments arranged by size:
2251 2007 1334 871 350 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3109 3771 10
Size: 54 1425 343 1277 662 3236
Fragments arranged by size:
3236 1425 1277 662 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2397 3161 4955 5314 5473 518
Size: 648 1231 764 1794 359 159 2042
Fragments arranged by size:
2042 1794 1231 764 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2608 3969 4158 4413 5090 6238 6483 1606
Size: 1002 1361 189 255 677 1148 245 2120
Fragments arranged by size:
2120 1361 1148 1002 677 255 245 189
NcoI C'CATG_G
Cuts at: 513 1065 2004 2084 3485 513
Size: 552 939 80 1401 4025
Fragments arranged by size:
4025 1401 939 552 80
NdeI CA'TA_TG
Cuts at: 387 2944 387
Size: 2557 4440
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6997
NotI GC'GGCC_GC
Cuts at: 4444 4444
Size: 6997
NspI r_CATG'y
Cuts at: 1132 1229 3359 4355 5900 1132
Size: 97 2130 996 1545 2229
Fragments arranged by size:
2229 2130 1545 996 97
PasI CC'CwG_GG
Cuts at: 1348 2104 2762 1348
Size: 756 658 5583
Fragments arranged by size:
5583 756 658
PciI A'CATG_T
Cuts at: 3355 5896 3355
Size: 2541 4456
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2085 2176 2930 2964 1108
Size: 977 91 754 34 5141
Fragments arranged by size:
5141 977 754 91 34
PfoI T'CCnGG_A
Cuts at: 2246 2790 3219 2246
Size: 544 429 6024
Fragments arranged by size:
6024 544 429
PmeI GTTT'AAAC
Cuts at: 4301 4301
Size: 6997
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5066 5098 6579 6611 5066
Size: 32 1481 32 5452
Fragments arranged by size:
5452 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2244 3193 3643 2244
Size: 949 450 5598
Fragments arranged by size:
5598 949 450
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6997
PsiI TTA'TAA
Cuts at: 4567 4567
Size: 6997
PspXI vC'TCGA_Gb
Cuts at: 3816 3816
Size: 6997
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2327 2359 2327
Size: 32 6965
PstI C_TGCA'G
Cuts at: 830 2234 2579 4349 830
Size: 1404 345 1770 3478
Fragments arranged by size:
3478 1770 1404 345
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6997
PvuII CAG'CTG
Cuts at: 3969 4158 3969
Size: 189 6808
SalI G'TCGA_C
Cuts at: 1936 4339 1936
Size: 2403 4594
SanDI GG'GwC_CC
Cuts at: 3193 3193
Size: 6997
SapI GCTCTTCn'nnn_
Cuts at: 3858 3858
Size: 6997
SbfI CC_TGCA'GG
Cuts at: 4349 4349
Size: 6997
ScaI AGT'ACT
Cuts at: 3560 5245 6957 3560
Size: 1685 1712 3600
Fragments arranged by size:
3600 1712 1685
SexAI A'CCwGG_T
Cuts at: 2820 2820
Size: 6997
SfcI C'TryA_G
Cuts at: 826 1045 2230 2575 4345 5480 6161 6352 826
Size: 219 1185 345 1770 1135 681 191 1471
Fragments arranged by size:
1770 1471 1185 1135 681 345 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6997
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6997
SmaI CCC'GGG
Cuts at: 1872 2508 4324 1872
Size: 636 1816 4545
Fragments arranged by size:
4545 1816 636
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2265 2685 3749 3816 5101 6002
Size: 197 926 322 420 1064 67 1285 901
Cuts at: 6002 6264 820
Size: 262 1553
Fragments arranged by size:
1553 1285 1064 926 901 420 322 262
197 67
SnaBI TAC'GTA
Cuts at: 493 4788 493
Size: 4295 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6997
SphI G_CATG'C
Cuts at: 1132 4355 1132
Size: 3223 3774
SspI AAT'ATT
Cuts at: 5 52 4921 5
Size: 47 4869 2081
Fragments arranged by size:
4869 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6997
StyI C'CwwG_G
Cuts at: 513 1065 2004 2084 3257 3485 4436 513
Size: 552 939 80 1173 228 951 3074
Fragments arranged by size:
3074 1173 951 939 552 228 80
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5024 5041 5194 5379 5824 325
Size: 1255 3444 17 153 185 445 1498
Fragments arranged by size:
3444 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3090 3558 4049
Size: 274 80 33 51 1443 1113 468 491
Cuts at: 4049 5243 6955 96
Size: 1194 1712 138
Fragments arranged by size:
1712 1443 1194 1113 491 468 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2171 2203 6957 6989 2171
Size: 32 4754 32 2179
Fragments arranged by size:
4754 2179 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2561 3005 4007 1316
Size: 1245 444 1002 4306
Fragments arranged by size:
4306 1245 1002 444
XbaI T'CTAG_A
Cuts at: 4333 4333
Size: 6997
XhoI C'TCGA_G
Cuts at: 1943 3816 1943
Size: 1873 5124
XmaI C'CCGG_G
Cuts at: 1870 2506 4322 1870
Size: 636 1816 4545
Fragments arranged by size:
4545 1816 636
XmnI GAAnn'nnTTC
Cuts at: 5126 5126
Size: 6997
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3118 4805 276
Size: 53 83 186 936 1584 1687 2468
Fragments arranged by size:
2468 1687 1584 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspXI PsrI PstI PvuI PvuII
SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspOMI RsrII SacI SacII SfiI SfoI SrfI
SwaI XcmI