Restriction map for
pFN21AE1429
(Circular) (Six-base) MAPSORT of: pFN21AE1429.seq Check: 791 from: 1 to: 6313
pFN21AE1429 6313 bp
With 182 enzymes: SgfI *
June 19, 2019 18:49 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4123 278
Size: 53 83 186 936 2587 2468
Fragments arranged by size:
2587 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2214 3656 1937
Size: 277 1442 4594
Fragments arranged by size:
4594 1442 277
Acc65I G'GTAC_C
Cuts at: 3634 3634
Size: 6313
AclI AA'CG_TT
Cuts at: 1563 4440 4813 1563
Size: 2877 373 3063
Fragments arranged by size:
3063 2877 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2093 2192 4376 5760 6028 1632
Size: 461 99 2184 1384 268 1917
Fragments arranged by size:
2184 1917 1384 461 268 99
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6116
AflIII A'CryG_T
Cuts at: 1203 3009 5212 6189 1203
Size: 1806 2203 977 1327
Fragments arranged by size:
2203 1806 1327 977
AgeI A'CCGG_T
Cuts at: 5144 5144
Size: 6313
AhdI GACnn_n'nnGTC
Cuts at: 3361 3541 5042 3361
Size: 180 1501 4632
Fragments arranged by size:
4632 1501 180
AleI CACnn'nnGTG
Cuts at: 1166 2438 3251 1166
Size: 1272 813 4228
Fragments arranged by size:
4228 1272 813
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6313
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2402 2403 2434 2435 2402
Size: 1 31 1 6280
Fragments arranged by size:
6280 31 1 1
AlwNI CAG_nnn'CTG
Cuts at: 2266 3343 4056 5628 2266
Size: 1077 713 1572 2951
Fragments arranged by size:
2951 1572 1077 713
ApaI G_GGCC'C
Cuts at: 2447 2447
Size: 6313
ApaLI G'TGCA_C
Cuts at: 2381 2738 4370 5526 2381
Size: 357 1632 1156 3168
Fragments arranged by size:
3168 1632 1156 357
ApoI r'AATT_y
Cuts at: 1493 3176 3622 3848 4000 6109 1493
Size: 1683 446 226 152 2109 1697
Fragments arranged by size:
2109 1697 1683 446 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4867 160
Size: 872 3835 1606
Fragments arranged by size:
3835 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6313
AvaI C'yCGr_G
Cuts at: 1870 1943 2331 3638 1870
Size: 73 388 1307 4545
Fragments arranged by size:
4545 1307 388 73
BamHI G'GATC_C
Cuts at: 3643 4010 3643
Size: 367 5946
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3390 3634 5089 618
Size: 325 217 627 1603 244 1455 1842
Fragments arranged by size:
1842 1603 1455 627 325 244 217
BanII G_rGCy'C
Cuts at: 1391 2447 2763 2980 3297 3632 1391
Size: 1056 316 217 317 335 4072
Fragments arranged by size:
4072 1056 335 317 316 217
BbeI G_GCGC'C
Cuts at: 3394 3394
Size: 6313
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3041 3050 928
Size: 971 1142 9 4191
Fragments arranged by size:
4191 1142 971 9
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4504 4538 1903
Size: 34 2567 34 3678
Fragments arranged by size:
3678 2567 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4205 5415 4205
Size: 1210 5103
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6313
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4924 136
Size: 107 122 71 631 605 3252 1525
Fragments arranged by size:
3252 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6308 6308
Size: 6313
BlpI GC'TnA_GC
Cuts at: 3730 3730
Size: 6313
Bme1580I G_kGCm'C
Cuts at: 1790 2385 2447 2742 2813 4374 5530 1790
Size: 595 62 295 71 1561 1156 2573
Fragments arranged by size:
2573 1561 1156 595 295 71 62
BmgBI CAC'GTC
Cuts at: 1318 3319 1318
Size: 2001 4312
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4044 5002 442
Size: 416 53 83 399 24 2627 958 1753
Fragments arranged by size:
2627 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6313
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2060 2087 2204 2900 3083 4973 1239
Size: 821 27 117 696 183 1890 2579
Fragments arranged by size:
2579 1890 821 696 183 117 27
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2462 4438 5303 5601 2462
Size: 1976 865 298 3174
Fragments arranged by size:
3174 1976 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2118 3414 4976 882
Size: 617 619 1296 1562 2219
Fragments arranged by size:
2219 1562 1296 619 617
BsaAI yAC'GTr
Cuts at: 493 1204 4104 493
Size: 711 2900 2702
Fragments arranged by size:
2900 2702 711
BsaBI GATnn'nnATC
Cuts at: 6307 6307
Size: 6313
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3391 4120 4502 5137
Size: 53 83 186 936 1858 729 382 635
Cuts at: 5137 275
Size: 1451
Fragments arranged by size:
1858 1451 936 729 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2029 2421 4746 5144 5418 5565 5976 2029
Size: 392 2325 398 274 147 411 2366
Fragments arranged by size:
2366 2325 411 398 392 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2060 2090 2255 2285 3191
Size: 30 165 30 657 30 165 30 906
Cuts at: 3191 3221 1178
Size: 30 4270
Fragments arranged by size:
4270 906 657 165 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2057 2180 2369 1186
Size: 871 123 189 5130
Fragments arranged by size:
5130 871 189 123
BseYI C'CCAG_C
Cuts at: 2084 2322 2396 3377 5516 6012 2084
Size: 238 74 981 2139 496 2385
Fragments arranged by size:
2385 2139 981 496 238 74
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2411 1163
Size: 573 675 5065
Fragments arranged by size:
5065 675 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3763 4524 5552 1540
Size: 275 186 1762 761 1028 2301
Fragments arranged by size:
2301 1762 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2385 2742 2763 2805 4374 4459 5530 2385
Size: 357 21 42 1569 85 1071 3168
Fragments arranged by size:
3168 1569 1071 357 85 42 21
BsmI GAATG_Cn'
Cuts at: 3468 3824 3917 3468
Size: 356 93 5864
Fragments arranged by size:
5864 356 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2385 2447 2742 2763 2805 2813 2980
Size: 399 595 62 295 21 42 8 167
Cuts at: 2980 3297 3632 4374 4459 5530 1391
Size: 317 335 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 595 399 335 317 295
167 85 62 42 21 8
BspEI T'CCGG_A
Cuts at: 2029 2421 5976 2029
Size: 392 3555 2366
Fragments arranged by size:
3555 2366 392
BspHI T'CATG_A
Cuts at: 4202 5932 4202
Size: 1730 4583
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3668 844
Size: 2824 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4200 1394
Size: 429 2377 3507
Fragments arranged by size:
3507 2377 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2710 4808 4982 65
Size: 1183 1462 2098 174 1396
Fragments arranged by size:
2098 1462 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4957 5144 1700
Size: 110 3147 187 2869
Fragments arranged by size:
3147 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2859 96
Size: 2763 3550
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6313
BssSI C'ACGA_G
Cuts at: 4373 5385 4373
Size: 1012 5301
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2266 2389 794
Size: 1472 123 4718
Fragments arranged by size:
4718 1472 123
BstBI TT'CG_AA
Cuts at: 5117 5117
Size: 6313
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6313
BstXI CCAn_nnnn'nTGG
Cuts at: 3253 3253
Size: 6313
BstYI r'GATC_y
Cuts at: 1972 2400 2488 2679 3643 4010 4395 4412 5864
Size: 428 88 191 964 367 385 17 1452
Cuts at: 5864 5950 5962 6308 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 964 428 385 367 346 191
88 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2543 2543
Size: 6313
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4822
Fragments arranged by size:
4822 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5574
Fragments arranged by size:
5574 683 56
BtsI GCAGTG_nn'
Cuts at: 2740 3140 3829 4623 4643 2740
Size: 400 689 794 20 4410
Fragments arranged by size:
4410 794 689 400 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6313
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3134 3169 529
Size: 35 2570 35 3673
Fragments arranged by size:
3673 2570 35 35
DraI TTT'AAA
Cuts at: 3617 3617
Size: 6313
DraIII CAC_nnn'GTG
Cuts at: 2389 3233 2389
Size: 844 5469
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5320 809
Size: 507 4004 1802
Fragments arranged by size:
4004 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2766 3000 3760
Size: 54 1425 50 275 18 936 234 760
Cuts at: 3760 4649 6278 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 936 889 760 275 234 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3760 1537
Size: 275 1948 4090
Fragments arranged by size:
4090 1948 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3323 3552 4243 1353
Size: 1970 229 691 3423
Fragments arranged by size:
3423 1970 691 229
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3362 4900 5274 5420 387
Size: 1064 138 1773 1538 374 146 1280
Fragments arranged by size:
1773 1538 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2761 2761
Size: 6313
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2060 2087 2093 2192 2204 2900 3083
Size: 393 428 27 6 99 12 696 183
Cuts at: 3083 4376 4973 5760 6028 1239
Size: 1293 597 787 268 1524
Fragments arranged by size:
1524 1293 787 696 597 428 393 268
183 99 27 12 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2951 3170 2951
Size: 219 6094
EcoO109I rG'GnC_Cy
Cuts at: 2444 3085 3163 3562 2444
Size: 641 78 399 5195
Fragments arranged by size:
5195 641 399 78
EcoRI G'AATT_C
Cuts at: 3176 3622 4000 3176
Size: 446 378 5489
Fragments arranged by size:
5489 446 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3067 3067
Size: 6313
FspI TGC'GCA
Cuts at: 4819 4819
Size: 6313
HaeII r_GCGC'y
Cuts at: 1678 3394 5460 1678
Size: 1716 2066 2531
Fragments arranged by size:
2531 2066 1716
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2042 2060 2074 2092 2255
Size: 32 135 32 470 18 14 18 163
Cuts at: 2255 2287 2967 2999 3341 3347 3373 3379 3527
Size: 32 680 32 342 6 26 6 148
Cuts at: 3527 3559 4954 4986 5028 5060 1373
Size: 32 1395 32 42 32 2626
Fragments arranged by size:
2626 1395 680 470 342 163 148 135
42 32 32 32 32 32 32 32
26 18 18 14 6 6
HincII GTy'rAC
Cuts at: 669 1657 1938 3657 3903 669
Size: 988 281 1719 246 3079
Fragments arranged by size:
3079 1719 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2105 748
Size: 1357 4956
HpaI GTT'AAC
Cuts at: 3903 3903
Size: 6313
KasI G'GCGC_C
Cuts at: 3390 3390
Size: 6313
KpnI G_GTAC'C
Cuts at: 3638 3638
Size: 6313
MfeI C'AATT_G
Cuts at: 3912 3912
Size: 6313
MluI A'CGCG_T
Cuts at: 6189 6189
Size: 6313
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2028 2121 2730 3134 5427 5611 1305
Size: 723 93 609 404 2293 184 2007
Fragments arranged by size:
2293 2007 723 609 404 184 93
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2768 3002 10
Size: 54 1425 343 936 234 3321
Fragments arranged by size:
3321 1425 936 343 234 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2438 2732 2796 2990 3008 3251 4271
Size: 648 1272 294 64 194 18 243 1020
Cuts at: 4271 4630 4789 518
Size: 359 159 2042
Fragments arranged by size:
2042 1272 1020 648 359 294 243 194
159 64 18
MspA1I CmG'CkG
Cuts at: 1606 3061 3729 4406 5554 5799 1606
Size: 1455 668 677 1148 245 2120
Fragments arranged by size:
2120 1455 1148 677 668 245
NarI GG'CG_CC
Cuts at: 3391 3391
Size: 6313
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4822
Fragments arranged by size:
4822 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6313
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6313
NotI GC'GGCC_GC
Cuts at: 3760 3760
Size: 6313
NspI r_CATG'y
Cuts at: 1132 1229 3013 3671 5216 1132
Size: 97 1784 658 1545 2229
Fragments arranged by size:
2229 1784 1545 658 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6313
PciI A'CATG_T
Cuts at: 3009 5212 3009
Size: 2203 4110
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3135 1108
Size: 2027 4286
PfoI T'CCnGG_A
Cuts at: 2625 2625
Size: 6313
PmeI GTTT'AAAC
Cuts at: 3617 3617
Size: 6313
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4382 4414 5895 5927 4382
Size: 32 1481 32 4768
Fragments arranged by size:
4768 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6313
PsiI TTA'TAA
Cuts at: 3883 3883
Size: 6313
PspOMI G'GGCC_C
Cuts at: 2443 2443
Size: 6313
PstI C_TGCA'G
Cuts at: 830 2262 2785 3665 830
Size: 1432 523 880 3478
Fragments arranged by size:
3478 1432 880 523
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6313
PvuII CAG'CTG
Cuts at: 3061 3061
Size: 6313
SacI G_AGCT'C
Cuts at: 2763 2763
Size: 6313
SalI G'TCGA_C
Cuts at: 1936 3655 1936
Size: 1719 4594
SbfI CC_TGCA'GG
Cuts at: 3665 3665
Size: 6313
ScaI AGT'ACT
Cuts at: 4561 6273 4561
Size: 1712 4601
SfcI C'TryA_G
Cuts at: 826 1045 2258 2781 3661 4796 5477 5668 826
Size: 219 1213 523 880 1135 681 191 1471
Fragments arranged by size:
1471 1213 1135 880 681 523 219 191
SfoI GGC'GCC
Cuts at: 3392 3392
Size: 6313
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6313
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6313
SmaI CCC'GGG
Cuts at: 1872 2333 3640 1872
Size: 461 1307 4545
Fragments arranged by size:
4545 1307 461
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2477 4417 5318 5580 820
Size: 197 926 534 1940 901 262 1553
Fragments arranged by size:
1940 1553 926 901 534 262 197
SnaBI TAC'GTA
Cuts at: 493 4104 493
Size: 3611 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6313
SphI G_CATG'C
Cuts at: 1132 3671 1132
Size: 2539 3774
SrfI GCCC'GGGC
Cuts at: 2333 2333
Size: 6313
SspI AAT'ATT
Cuts at: 5 52 4237 5
Size: 47 4185 2081
Fragments arranged by size:
4185 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6313
StyI C'CwwG_G
Cuts at: 513 1065 2004 2634 2952 3081 3159 3752 513
Size: 552 939 630 318 129 78 593 3074
Fragments arranged by size:
3074 939 630 593 552 318 129 78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2100 2336 3138 4340 4357 4510 4695
Size: 1255 520 236 802 1202 17 153 185
Cuts at: 4695 5140 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1202 802 520 445 236 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2187 2655 2859
Size: 274 80 33 51 1443 210 468 204
Cuts at: 2859 4559 6271 96
Size: 1700 1712 138
Fragments arranged by size:
1712 1700 1443 468 274 210 204 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2480 2512 6273 6305 2480
Size: 32 3761 32 2488
Fragments arranged by size:
3761 2488 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2839 1316
Size: 1523 4790
XbaI T'CTAG_A
Cuts at: 3649 3649
Size: 6313
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3132 3136 3166 3433 3132
Size: 4 30 267 6012
Fragments arranged by size:
6012 267 30 4
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6313
XmaI C'CCGG_G
Cuts at: 1870 2331 3638 1870
Size: 461 1307 4545
Fragments arranged by size:
4545 1307 461
XmnI GAAnn'nnTTC
Cuts at: 2936 4442 2936
Size: 1506 4807
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4121 276
Size: 53 83 186 936 2587 2468
Fragments arranged by size:
2587 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstZ17I EcoRV FseI FspAI NaeI
NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI
RsrII SacII SanDI SapI SexAI SfiI SwaI