Restriction map for
pFN21AE1425
(Circular) (Six-base) MAPSORT of: pFN21AE1425.seq Check: 5843 from: 1 to: 5797
pFN21AE1425 5797 bp
With 182 enzymes: SgfI *
August 16, 2010 14:46 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3607 278
Size: 53 83 186 936 2071 2468
Fragments arranged by size:
2468 2071 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2322 3140 1937
Size: 385 818 4594
Fragments arranged by size:
4594 818 385
Acc65I G'GTAC_C
Cuts at: 3118 3118
Size: 5797
AclI AA'CG_TT
Cuts at: 1563 3924 4297 1563
Size: 2361 373 3063
Fragments arranged by size:
3063 2361 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3860 5244 5512 1632
Size: 2228 1384 268 1917
Fragments arranged by size:
2228 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5600
AflIII A'CryG_T
Cuts at: 1203 4696 5673 1203
Size: 3493 977 1327
Fragments arranged by size:
3493 1327 977
AgeI A'CCGG_T
Cuts at: 4628 4628
Size: 5797
AhdI GACnn_n'nnGTC
Cuts at: 4526 4526
Size: 5797
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5797
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5797
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2291 2323 2291
Size: 32 5765
AlwNI CAG_nnn'CTG
Cuts at: 3540 5112 3540
Size: 1572 4225
ApaI G_GGCC'C
Cuts at: 2047 2570 2047
Size: 523 5274
ApaLI G'TGCA_C
Cuts at: 3854 5010 3854
Size: 1156 4641
ApoI r'AATT_y
Cuts at: 1493 2062 3106 3332 3484 5593 1493
Size: 569 1044 226 152 2109 1697
Fragments arranged by size:
2109 1697 1044 569 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4351 160
Size: 872 3319 1606
Fragments arranged by size:
3319 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5797
AvaI C'yCGr_G
Cuts at: 1870 1943 3122 1870
Size: 73 1179 4545
Fragments arranged by size:
4545 1179 73
BamHI G'GATC_C
Cuts at: 2056 2287 3127 3494 2056
Size: 231 840 367 4359
Fragments arranged by size:
4359 840 367 231
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2624 2984 3118 4573 618
Size: 325 217 627 837 360 134 1455 1842
Fragments arranged by size:
1842 1455 837 627 360 325 217 134
BanII G_rGCy'C
Cuts at: 1391 2047 2570 3116 1391
Size: 656 523 546 4072
Fragments arranged by size:
4072 656 546 523
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2858 928
Size: 971 959 3867
Fragments arranged by size:
3867 971 959
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3988 4022 1903
Size: 34 50 34 1967 34 3678
Fragments arranged by size:
3678 1967 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3689 4899 3689
Size: 1210 4587
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5797
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4408 136
Size: 107 122 71 631 605 2736 1525
Fragments arranged by size:
2736 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5792 5792
Size: 5797
BlpI GC'TnA_GC
Cuts at: 3214 3214
Size: 5797
Bme1580I G_kGCm'C
Cuts at: 1790 2047 2124 2570 3858 5014 1790
Size: 257 77 446 1288 1156 2573
Fragments arranged by size:
2573 1288 1156 446 257 77
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5797
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2162 2286 3528
Size: 416 53 83 399 24 745 124 1242
Cuts at: 3528 4486 442
Size: 958 1753
Fragments arranged by size:
1753 1242 958 745 416 399 124 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5797
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2202 2676 4457 1239
Size: 963 474 1781 2579
Fragments arranged by size:
2579 1781 963 474
Bpu10I CC'TnA_GC
Cuts at: 2312 2746 2312
Size: 434 5363
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2956 3922 4787 5085 2956
Size: 966 865 298 3668
Fragments arranged by size:
3668 966 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2753 4460 882
Size: 617 1254 1707 2219
Fragments arranged by size:
2219 1707 1254 617
BsaAI yAC'GTr
Cuts at: 493 1204 3588 493
Size: 711 2384 2702
Fragments arranged by size:
2702 2384 711
BsaBI GATnn'nnATC
Cuts at: 5791 5791
Size: 5797
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2325 3604 3986 4621
Size: 53 83 186 936 792 1279 382 635
Cuts at: 4621 275
Size: 1451
Fragments arranged by size:
1451 1279 936 792 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4230 4628 4902 5049 5460 4230
Size: 398 274 147 411 4567
Fragments arranged by size:
4567 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2093 2123 2147 2177 2291
Size: 30 165 30 690 30 24 30 114
Cuts at: 2291 2321 2649 2679 2787 2817 1178
Size: 30 328 30 108 30 4158
Fragments arranged by size:
4158 690 328 165 114 108 30 30
30 30 30 30 30 24
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2270 2347 2575 2808 3026 1186
Size: 1084 77 228 233 218 3957
Fragments arranged by size:
3957 1084 233 228 218 77
BseYI C'CCAG_C
Cuts at: 2400 5000 5496 2400
Size: 2600 496 2701
Fragments arranged by size:
2701 2600 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2099 2110 1163
Size: 573 363 11 4850
Fragments arranged by size:
4850 573 363 11
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3247 4008 5036 1540
Size: 275 186 1246 761 1028 2301
Fragments arranged by size:
2301 1246 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2595 3858 3943 5014 2595
Size: 1263 85 1071 3378
Fragments arranged by size:
3378 1263 1071 85
BsmI GAATG_Cn'
Cuts at: 3308 3401 3308
Size: 93 5704
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2047 2124 2570 2595 3116 3858 3943
Size: 399 257 77 446 25 521 742 85
Cuts at: 3943 5014 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 521 446 399 257 85
77 25
BspEI T'CCGG_A
Cuts at: 5460 5460
Size: 5797
BspHI T'CATG_A
Cuts at: 3686 5416 3686
Size: 1730 4067
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2498 3152 844
Size: 1654 654 3489
Fragments arranged by size:
3489 1654 654
BsrBI CCG'CTC
Cuts at: 1394 1823 3684 1394
Size: 429 1861 3507
Fragments arranged by size:
3507 1861 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4292 4466 65
Size: 1183 3044 174 1396
Fragments arranged by size:
3044 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4441 4628 1700
Size: 110 2631 187 2869
Fragments arranged by size:
2869 2631 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5797
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5797
BssSI C'ACGA_G
Cuts at: 2117 3857 4869 2117
Size: 1740 1012 3045
Fragments arranged by size:
3045 1740 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5797
BstBI TT'CG_AA
Cuts at: 4601 4601
Size: 5797
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5797
BstXI CCAn_nnnn'nTGG
Cuts at: 2067 2563 2067
Size: 496 5301
BstYI r'GATC_y
Cuts at: 1972 2056 2287 3127 3494 3879 3896 5348 5434
Size: 84 231 840 367 385 17 1452 86
Cuts at: 5434 5446 5792 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 840 385 367 346 231 86
84 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2606 2620 513
Size: 552 1541 14 3690
Fragments arranged by size:
3690 1541 552 14
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5058
Fragments arranged by size:
5058 683 56
BtsI GCAGTG_nn'
Cuts at: 2889 3313 4107 4127 2889
Size: 424 794 20 4559
Fragments arranged by size:
4559 794 424 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5797
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5762
DraI TTT'AAA
Cuts at: 3101 3101
Size: 5797
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4804 809
Size: 507 3488 1802
Fragments arranged by size:
3488 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2223 2470 3244
Size: 54 1425 50 275 18 393 247 774
Cuts at: 3244 4133 5762 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 774 393 275 247 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3244 1537
Size: 275 1432 4090
Fragments arranged by size:
4090 1432 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3727 1353
Size: 2374 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2264 4384 4758 4904 387
Size: 1064 138 675 2120 374 146 1280
Fragments arranged by size:
2120 1280 1064 675 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2202 2676 3860 4457 5244 5512 1239
Size: 393 570 474 1184 597 787 268 1524
Fragments arranged by size:
1524 1184 787 597 570 474 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2910 2910
Size: 5797
EcoO109I rG'GnC_Cy
Cuts at: 2043 2044 2567 3090 2043
Size: 1 523 523 4750
Fragments arranged by size:
4750 523 523 1
EcoRI G'AATT_C
Cuts at: 3106 3484 3106
Size: 378 5419
FspI TGC'GCA
Cuts at: 4303 4303
Size: 5797
HaeII r_GCGC'y
Cuts at: 1678 4944 1678
Size: 3266 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2147 2179 2262 2294 4438
Size: 32 135 32 575 32 83 32 2144
Cuts at: 4438 4470 4512 4544 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2144 575 135 83 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3141 3387 669
Size: 988 281 1203 246 3079
Fragments arranged by size:
3079 1203 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5797
HpaI GTT'AAC
Cuts at: 3387 3387
Size: 5797
KpnI G_GTAC'C
Cuts at: 3122 3122
Size: 5797
MfeI C'AATT_G
Cuts at: 3396 3396
Size: 5797
MluI A'CGCG_T
Cuts at: 5673 5673
Size: 5797
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4911 5095 1305
Size: 3606 184 2007
Fragments arranged by size:
3606 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2225 10
Size: 54 1425 343 393 3582
Fragments arranged by size:
3582 1425 393 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3755 4114 4273 518
Size: 648 2589 359 159 2042
Fragments arranged by size:
2589 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2011 2549 3213 3890 5038 5283 1606
Size: 405 538 664 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 664 538 405 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5245
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5797
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5797
NotI GC'GGCC_GC
Cuts at: 3244 3244
Size: 5797
NspI r_CATG'y
Cuts at: 1132 1229 2417 3155 4700 1132
Size: 97 1188 738 1545 2229
Fragments arranged by size:
2229 1545 1188 738 97
PasI CC'CwG_GG
Cuts at: 1348 2563 1348
Size: 1215 4582
PciI A'CATG_T
Cuts at: 4696 4696
Size: 5797
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2174 2723 2741 1108
Size: 1066 549 18 4164
Fragments arranged by size:
4164 1066 549 18
PfoI T'CCnGG_A
Cuts at: 2187 2734 2187
Size: 547 5250
PmeI GTTT'AAAC
Cuts at: 3101 3101
Size: 5797
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2291 2323 3866 3898 5379 5411 2291
Size: 32 1543 32 1481 32 2677
Fragments arranged by size:
2677 1543 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3090 3090
Size: 5797
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5797
PsiI TTA'TAA
Cuts at: 3367 3367
Size: 5797
PspOMI G'GGCC_C
Cuts at: 2043 2566 2043
Size: 523 5274
PstI C_TGCA'G
Cuts at: 830 3149 830
Size: 2319 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5797
PvuII CAG'CTG
Cuts at: 2549 2549
Size: 5797
SalI G'TCGA_C
Cuts at: 1936 3139 1936
Size: 1203 4594
SbfI CC_TGCA'GG
Cuts at: 3149 3149
Size: 5797
ScaI AGT'ACT
Cuts at: 4045 5757 4045
Size: 1712 4085
SfcI C'TryA_G
Cuts at: 826 1045 3145 4280 4961 5152 826
Size: 219 2100 1135 681 191 1471
Fragments arranged by size:
2100 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5797
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5797
SmaI CCC'GGG
Cuts at: 1872 3124 1872
Size: 1252 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2971 3901 4802 5064 820
Size: 197 926 1028 930 901 262 1553
Fragments arranged by size:
1553 1028 930 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3588 493
Size: 3095 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5797
SphI G_CATG'C
Cuts at: 1132 3155 1132
Size: 2023 3774
SspI AAT'ATT
Cuts at: 5 52 3721 5
Size: 47 3669 2081
Fragments arranged by size:
3669 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5797
StyI C'CwwG_G
Cuts at: 513 1065 2281 3057 3236 513
Size: 552 1216 776 179 3074
Fragments arranged by size:
3074 1216 776 552 179
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2915 3824 3841 3994 4179 4624 325
Size: 1255 1335 909 17 153 185 445 1498
Fragments arranged by size:
1498 1335 1255 909 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2248 4043 5755
Size: 274 80 33 51 1443 271 1795 1712
Cuts at: 5755 96
Size: 138
Fragments arranged by size:
1795 1712 1443 274 271 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2093 2125 2649 2681 2689 2721 5757 5789 2093
Size: 32 524 32 8 32 3036 32 2101
Fragments arranged by size:
3036 2101 524 32 32 32 32 8
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5797
XbaI T'CTAG_A
Cuts at: 2461 3133 2461
Size: 672 5125
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2770 2881 2770
Size: 111 5686
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5797
XmaI C'CCGG_G
Cuts at: 1870 3122 1870
Size: 1252 4545
XmnI GAAnn'nnTTC
Cuts at: 3926 3926
Size: 5797
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3605 276
Size: 53 83 186 936 2071 2468
Fragments arranged by size:
2468 2071 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspXI PsrI RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI