Restriction map for
pFN21AE1424
(Circular) (Six-base) MAPSORT of: pFN21AE1424.seq Check: 966 from: 1 to: 6373
pFN21AE1424 6373 bp
With 182 enzymes: SgfI *
July 6, 2012 17:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4183 278
Size: 53 83 186 936 2647 2468
Fragments arranged by size:
2647 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3716 1937
Size: 1779 4594
Acc65I G'GTAC_C
Cuts at: 3694 3694
Size: 6373
AclI AA'CG_TT
Cuts at: 1563 2067 4500 4873 1563
Size: 504 2433 373 3063
Fragments arranged by size:
3063 2433 504 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2210 2262 2704 2915 4436 5820 6088 1632
Size: 578 52 442 211 1521 1384 268 1917
Fragments arranged by size:
1917 1521 1384 578 442 268 211 52
AfeI AGC'GCT
Cuts at: 3173 3173
Size: 6373
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6176
AflIII A'CryG_T
Cuts at: 1203 5272 6249 1203
Size: 4069 977 1327
Fragments arranged by size:
4069 1327 977
AgeI A'CCGG_T
Cuts at: 5204 5204
Size: 6373
AhdI GACnn_n'nnGTC
Cuts at: 5102 5102
Size: 6373
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6373
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6373
AlwNI CAG_nnn'CTG
Cuts at: 2774 3563 4116 5688 2774
Size: 789 553 1572 3459
Fragments arranged by size:
3459 1572 789 553
ApaLI G'TGCA_C
Cuts at: 4430 5586 4430
Size: 1156 5217
ApoI r'AATT_y
Cuts at: 1493 2337 2826 3069 3548 3682 3908 4060 6169
Size: 844 489 243 479 134 226 152 2109
Cuts at: 6169 1493
Size: 1697
Fragments arranged by size:
2109 1697 844 489 479 243 226 152
134
AseI AT'TA_AT
Cuts at: 160 1032 2451 4927 160
Size: 872 1419 2476 1606
Fragments arranged by size:
2476 1606 1419 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6373
AvaI C'yCGr_G
Cuts at: 1870 1943 3300 3489 3698 1870
Size: 73 1357 189 209 4545
Fragments arranged by size:
4545 1357 209 189 73
BamHI G'GATC_C
Cuts at: 2666 3703 4070 2666
Size: 1037 367 4969
Fragments arranged by size:
4969 1037 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2849 3694 5149 618
Size: 325 217 627 1062 845 1455 1842
Fragments arranged by size:
1842 1455 1062 845 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3664 3692 1391
Size: 2273 28 4072
Fragments arranged by size:
4072 2273 28
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2466 928
Size: 971 567 4835
Fragments arranged by size:
4835 971 567
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2358 2392 4564 4598 1903
Size: 34 421 34 2172 34 3678
Fragments arranged by size:
3678 2172 421 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4265 5475 4265
Size: 1210 5163
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6373
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4984 136
Size: 107 122 71 631 605 3312 1525
Fragments arranged by size:
3312 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3388 6368 3388
Size: 2980 3393
BlpI GC'TnA_GC
Cuts at: 3790 3790
Size: 6373
Bme1580I G_kGCm'C
Cuts at: 1790 4434 5590 1790
Size: 2644 1156 2573
Fragments arranged by size:
2644 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6373
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2052 2441 4104
Size: 416 53 83 399 24 635 389 1663
Cuts at: 4104 5062 442
Size: 958 1753
Fragments arranged by size:
1753 1663 958 635 416 399 389 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6373
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2219 2994 3678 5033 1239
Size: 980 775 684 1355 2579
Fragments arranged by size:
2579 1355 980 775 684
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4498 5363 5661 4498
Size: 865 298 5210
Fragments arranged by size:
5210 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3303 5036 882
Size: 617 1804 1733 2219
Fragments arranged by size:
2219 1804 1733 617
BsaAI yAC'GTr
Cuts at: 493 1204 3137 4164 493
Size: 711 1933 1027 2702
Fragments arranged by size:
2702 1933 1027 711
BsaBI GATnn'nnATC
Cuts at: 3092 6367 3092
Size: 3275 3098
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4180 4562 5197 275
Size: 53 83 186 936 2647 382 635 1451
Fragments arranged by size:
2647 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4806 5204 5478 5625 6036 4806
Size: 398 274 147 411 5143
Fragments arranged by size:
5143 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6148
Fragments arranged by size:
6148 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6373
BseYI C'CCAG_C
Cuts at: 5576 6072 5576
Size: 496 5877
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5800
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3823 4584 5612 1540
Size: 275 186 1822 761 1028 2301
Fragments arranged by size:
2301 1822 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4434 4519 5590 4434
Size: 85 1071 5217
Fragments arranged by size:
5217 1071 85
BsiWI C'GTAC_G
Cuts at: 3113 3113
Size: 6373
BsmI GAATG_Cn'
Cuts at: 3884 3977 3884
Size: 93 6280
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3664 3692 4434 4519 5590 1391
Size: 399 1874 28 742 85 1071 2174
Fragments arranged by size:
2174 1874 1071 742 399 85 28
BspEI T'CCGG_A
Cuts at: 6036 6036
Size: 6373
BspHI T'CATG_A
Cuts at: 4262 5992 4262
Size: 1730 4643
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3728 844
Size: 2884 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2058 4260 1394
Size: 429 235 2202 3507
Fragments arranged by size:
3507 2202 429 235
BsrDI GCAATG_nn'
Cuts at: 65 1248 2958 3039 3484 4868 5042 65
Size: 1183 1710 81 445 1384 174 1396
Fragments arranged by size:
1710 1396 1384 1183 445 174 81
BsrFI r'CCGG_y
Cuts at: 1700 1810 5017 5204 1700
Size: 110 3207 187 2869
Fragments arranged by size:
3207 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2752 96
Size: 2656 3717
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6373
BssSI C'ACGA_G
Cuts at: 4433 5445 4433
Size: 1012 5361
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2705 2849 3326 3368 794
Size: 1911 144 477 42 3799
Fragments arranged by size:
3799 1911 477 144 42
BstBI TT'CG_AA
Cuts at: 3580 5177 3580
Size: 1597 4776
BstEII G'GTnAC_C
Cuts at: 1184 2030 1184
Size: 846 5527
BstXI CCAn_nnnn'nTGG
Cuts at: 3391 3391
Size: 6373
BstYI r'GATC_y
Cuts at: 1972 2666 2835 3388 3703 4070 4455 4472 5924
Size: 694 169 553 315 367 385 17 1452
Cuts at: 5924 6010 6022 6368 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 694 553 385 367 346 315
169 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2605 513
Size: 552 939 601 4281
Fragments arranged by size:
4281 939 601 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5634
Fragments arranged by size:
5634 683 56
BtsI GCAGTG_nn'
Cuts at: 2394 3889 4683 4703 2394
Size: 1495 794 20 4064
Fragments arranged by size:
4064 1495 794 20
ClaI AT'CG_AT
Cuts at: 1554 3125 1554
Size: 1571 4802
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3207 3242 529
Size: 35 2643 35 3660
Fragments arranged by size:
3660 2643 35 35
DraI TTT'AAA
Cuts at: 2940 3677 2940
Size: 737 5636
DraIII CAC_nnn'GTG
Cuts at: 3194 3194
Size: 6373
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2470 5380 809
Size: 507 1154 2910 1802
Fragments arranged by size:
2910 1802 1154 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2997 3820 4709
Size: 54 1425 50 275 18 1167 823 889
Cuts at: 4709 6338 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1167 889 823 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3820 1537
Size: 275 2008 4090
Fragments arranged by size:
4090 2008 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2431 2926 3197 4303 1353
Size: 1078 495 271 1106 3423
Fragments arranged by size:
3423 1106 1078 495 271
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4960 5334 5480 387
Size: 1064 138 3371 374 146 1280
Fragments arranged by size:
3371 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2210 2219 2262 2704 2915 2994 3678
Size: 393 578 9 43 442 211 79 684
Cuts at: 3678 4436 5033 5820 6088 1239
Size: 758 597 787 268 1524
Fragments arranged by size:
1524 787 758 684 597 578 442 393
268 211 79 43 9
EcoO109I rG'GnC_Cy
Cuts at: 2012 2947 2012
Size: 935 5438
EcoRI G'AATT_C
Cuts at: 2826 3682 4060 2826
Size: 856 378 5139
Fragments arranged by size:
5139 856 378
EcoRV GAT'ATC
Cuts at: 2812 2812
Size: 6373
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3275 3275
Size: 6373
FspI TGC'GCA
Cuts at: 4879 4879
Size: 6373
HaeII r_GCGC'y
Cuts at: 1678 3175 5520 1678
Size: 1497 2345 2531
Fragments arranged by size:
2531 2345 1497
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3206 3238 5014 5046 5088
Size: 32 135 32 1634 32 1776 32 42
Cuts at: 5088 5120 1373
Size: 32 2626
Fragments arranged by size:
2626 1776 1634 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2419 2581 2991 3433 3717 3963
Size: 988 281 481 162 410 442 284 246
Cuts at: 3963 669
Size: 3079
Fragments arranged by size:
3079 988 481 442 410 284 281 246
162
HindIII A'AGCT_T
Cuts at: 748 2744 2967 748
Size: 1996 223 4154
Fragments arranged by size:
4154 1996 223
HpaI GTT'AAC
Cuts at: 2419 3433 3963 2419
Size: 1014 530 4829
Fragments arranged by size:
4829 1014 530
KpnI G_GTAC'C
Cuts at: 3698 3698
Size: 6373
MfeI C'AATT_G
Cuts at: 3972 3972
Size: 6373
MluI A'CGCG_T
Cuts at: 6249 6249
Size: 6373
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2242 5487 5671 1305
Size: 937 3245 184 2007
Fragments arranged by size:
3245 2007 937 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2999 10
Size: 54 1425 343 1167 3384
Fragments arranged by size:
3384 1425 1167 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4331 4690 4849 518
Size: 648 3165 359 159 2042
Fragments arranged by size:
3165 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3789 4466 5614 5859 1606
Size: 2183 677 1148 245 2120
Fragments arranged by size:
2183 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2605 513
Size: 552 939 601 4281
Fragments arranged by size:
4281 939 601 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6373
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6373
NotI GC'GGCC_GC
Cuts at: 3820 3820
Size: 6373
NspI r_CATG'y
Cuts at: 1132 1229 2680 3731 5276 1132
Size: 97 1451 1051 1545 2229
Fragments arranged by size:
2229 1545 1451 1051 97
PasI CC'CwG_GG
Cuts at: 1348 2671 1348
Size: 1323 5050
PciI A'CATG_T
Cuts at: 5272 5272
Size: 6373
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6373
PmeI GTTT'AAAC
Cuts at: 3677 3677
Size: 6373
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4442 4474 5955 5987 4442
Size: 32 1481 32 4828
Fragments arranged by size:
4828 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2947 2947
Size: 6373
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6373
PsiI TTA'TAA
Cuts at: 3943 3943
Size: 6373
PstI C_TGCA'G
Cuts at: 830 2080 2218 3725 830
Size: 1250 138 1507 3478
Fragments arranged by size:
3478 1507 1250 138
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6373
SalI G'TCGA_C
Cuts at: 1936 3715 1936
Size: 1779 4594
SbfI CC_TGCA'GG
Cuts at: 3725 3725
Size: 6373
ScaI AGT'ACT
Cuts at: 4621 6333 4621
Size: 1712 4661
SfcI C'TryA_G
Cuts at: 826 1045 2076 2214 3246 3721 4856 5537 5728
Size: 219 1031 138 1032 475 1135 681 191
Cuts at: 5728 826
Size: 1471
Fragments arranged by size:
1471 1135 1032 1031 681 475 219 191
138
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6373
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6373
SmaI CCC'GGG
Cuts at: 1872 3700 1872
Size: 1828 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3489 4477 5378 5640 820
Size: 197 926 1546 988 901 262 1553
Fragments arranged by size:
1553 1546 988 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4164 493
Size: 3671 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6373
SphI G_CATG'C
Cuts at: 1132 2680 3731 1132
Size: 1548 1051 3774
Fragments arranged by size:
3774 1548 1051
SspI AAT'ATT
Cuts at: 5 52 2095 2593 4297 5
Size: 47 2043 498 1704 2081
Fragments arranged by size:
2081 2043 1704 498 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6373
StyI C'CwwG_G
Cuts at: 513 1065 2004 2605 2817 3812 513
Size: 552 939 601 212 995 3074
Fragments arranged by size:
3074 995 939 601 552 212
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4400 4417 4570 4755 5200 325
Size: 1255 2820 17 153 185 445 1498
Fragments arranged by size:
2820 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2530 2752 4619
Size: 274 80 33 51 1443 553 222 1867
Cuts at: 4619 6331 96
Size: 1712 138
Fragments arranged by size:
1867 1712 1443 553 274 222 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6333 6365 6333
Size: 32 6341
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6373
XbaI T'CTAG_A
Cuts at: 3709 3709
Size: 6373
XhoI C'TCGA_G
Cuts at: 1943 3489 1943
Size: 1546 4827
XmaI C'CCGG_G
Cuts at: 1870 3698 1870
Size: 1828 4545
XmnI GAAnn'nnTTC
Cuts at: 2275 4502 2275
Size: 2227 4146
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4181 276
Size: 53 83 186 936 2647 2468
Fragments arranged by size:
2647 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsmBI BstZ17I Bsu36I EcoICRI EcoNI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI