Restriction map for
pFN21AE1422
(Circular) (Six-base) MAPSORT of: pFN21AE1422.seq Check: 950 from: 1 to: 5980
pFN21AE1422 5980 bp
With 182 enzymes: SgfI *
August 16, 2010 14:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3790 278
Size: 53 83 186 936 2254 2468
Fragments arranged by size:
2468 2254 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3323 1937
Size: 1386 4594
Acc65I G'GTAC_C
Cuts at: 3301 3301
Size: 5980
AclI AA'CG_TT
Cuts at: 1563 4107 4480 1563
Size: 2544 373 3063
Fragments arranged by size:
3063 2544 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2732 4043 5427 5695 1632
Size: 1100 1311 1384 268 1917
Fragments arranged by size:
1917 1384 1311 1100 268
AfeI AGC'GCT
Cuts at: 3037 3227 3037
Size: 190 5790
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5783
AflIII A'CryG_T
Cuts at: 1203 4879 5856 1203
Size: 3676 977 1327
Fragments arranged by size:
3676 1327 977
AgeI A'CCGG_T
Cuts at: 4811 4811
Size: 5980
AhdI GACnn_n'nnGTC
Cuts at: 4709 4709
Size: 5980
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5980
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5980
AlwNI CAG_nnn'CTG
Cuts at: 3723 5295 3723
Size: 1572 4408
ApaLI G'TGCA_C
Cuts at: 3266 4037 5193 3266
Size: 771 1156 4053
Fragments arranged by size:
4053 1156 771
ApoI r'AATT_y
Cuts at: 1493 3289 3515 3667 5776 1493
Size: 1796 226 152 2109 1697
Fragments arranged by size:
2109 1796 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4534 160
Size: 872 3502 1606
Fragments arranged by size:
3502 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5980
AvaI C'yCGr_G
Cuts at: 1870 1943 2074 3100 3305 1870
Size: 73 131 1026 205 4545
Fragments arranged by size:
4545 1026 205 131 73
AvrII C'CTAG_G
Cuts at: 2122 2122
Size: 5980
BamHI G'GATC_C
Cuts at: 3310 3677 3310
Size: 367 5613
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2537 3301 4756 618
Size: 325 217 627 750 764 1455 1842
Fragments arranged by size:
1842 1455 764 750 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2081 2270 3299 1391
Size: 690 189 1029 4072
Fragments arranged by size:
4072 1029 690 189
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2658 2958 928
Size: 971 759 300 3950
Fragments arranged by size:
3950 971 759 300
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4171 4205 1903
Size: 34 2234 34 3678
Fragments arranged by size:
3678 2234 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3872 5082 3872
Size: 1210 4770
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5980
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4591 136
Size: 107 122 71 631 605 2919 1525
Fragments arranged by size:
2919 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5975 5975
Size: 5980
BlpI GC'TnA_GC
Cuts at: 3397 3397
Size: 5980
Bme1580I G_kGCm'C
Cuts at: 1790 3270 4041 5197 1790
Size: 1480 771 1156 2573
Fragments arranged by size:
2573 1480 1156 771
BmgBI CAC'GTC
Cuts at: 1318 2832 1318
Size: 1514 4466
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3711 4669 442
Size: 416 53 83 399 24 2294 958 1753
Fragments arranged by size:
2294 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5980
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2369 2450 2603 3070 3245 4640 1239
Size: 1130 81 153 467 175 1395 2579
Fragments arranged by size:
2579 1395 1130 467 175 153 81
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2714 4105 4970 5268 2714
Size: 1391 865 298 3426
Fragments arranged by size:
3426 1391 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2933 4643 882
Size: 617 1434 1710 2219
Fragments arranged by size:
2219 1710 1434 617
BsaAI yAC'GTr
Cuts at: 493 1204 3771 493
Size: 711 2567 2702
Fragments arranged by size:
2702 2567 711
BsaBI GATnn'nnATC
Cuts at: 5974 5974
Size: 5980
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3787 4169 4804 275
Size: 53 83 186 936 2254 382 635 1451
Fragments arranged by size:
2254 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2534 2563 4413 4811 5085 5232 5643 2534
Size: 29 1850 398 274 147 411 2871
Fragments arranged by size:
2871 1850 411 398 274 147 29
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2303 2333 3260 3290 1178
Size: 30 165 30 900 30 927 30 3868
Fragments arranged by size:
3868 927 900 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2150 2441 2570 2602 2654 2818 3008 3077
Size: 964 291 129 32 52 164 190 69
Cuts at: 3077 3161 3164 3230 1186
Size: 84 3 66 3936
Fragments arranged by size:
3936 964 291 190 164 129 84 69
66 52 32 3
BseYI C'CCAG_C
Cuts at: 2081 2111 2445 2776 5183 5679 2081
Size: 30 334 331 2407 496 2382
Fragments arranged by size:
2407 2382 496 334 331 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2004 2240 2579 3266 1163
Size: 573 268 236 339 687 3877
Fragments arranged by size:
3877 687 573 339 268 236
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3430 4191 5219 1540
Size: 275 186 1429 761 1028 2301
Fragments arranged by size:
2301 1429 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2181 2478 3261 3270 4041 4126 5197 2181
Size: 297 783 9 771 85 1071 2964
Fragments arranged by size:
2964 1071 783 771 297 85 9
BsmI GAATG_Cn'
Cuts at: 3491 3584 3491
Size: 93 5887
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2081 2181 2270 2478 3261 3270 3299
Size: 399 291 100 89 208 783 9 29
Cuts at: 3299 4041 4126 5197 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 783 742 399 291 208 100
89 85 29 9
BspEI T'CCGG_A
Cuts at: 2563 5643 2563
Size: 3080 2900
BspHI T'CATG_A
Cuts at: 3869 5599 3869
Size: 1730 4250
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2964 3335 844
Size: 2120 371 3489
Fragments arranged by size:
3489 2120 371
BsrBI CCG'CTC
Cuts at: 1394 1823 3867 1394
Size: 429 2044 3507
Fragments arranged by size:
3507 2044 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4475 4649 65
Size: 1183 3227 174 1396
Fragments arranged by size:
3227 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4624 4811 1700
Size: 110 2814 187 2869
Fragments arranged by size:
2869 2814 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5980
BssHII G'CGCG_C
Cuts at: 1925 2380 1925
Size: 455 5525
BssSI C'ACGA_G
Cuts at: 4040 5052 4040
Size: 1012 4968
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5980
BstBI TT'CG_AA
Cuts at: 4784 4784
Size: 5980
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5980
BstXI CCAn_nnnn'nTGG
Cuts at: 2045 3040 2045
Size: 995 4985
BstYI r'GATC_y
Cuts at: 1972 2499 2702 3310 3677 4062 4079 5531 5617
Size: 527 203 608 367 385 17 1452 86
Cuts at: 5617 5629 5975 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 608 527 385 367 346 203
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2854 2854
Size: 5980
BtgI C'CryG_G
Cuts at: 513 1065 2004 2541 2601 513
Size: 552 939 537 60 3892
Fragments arranged by size:
3892 939 552 537 60
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5241
Fragments arranged by size:
5241 683 56
BtsI GCAGTG_nn'
Cuts at: 2414 2971 3496 4290 4310 2414
Size: 557 525 794 20 4084
Fragments arranged by size:
4084 794 557 525 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5980
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5945
DraI TTT'AAA
Cuts at: 3284 3284
Size: 5980
DraIII CAC_nnn'GTG
Cuts at: 2602 2602
Size: 5980
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4987 809
Size: 507 3671 1802
Fragments arranged by size:
3671 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3427 4316 5945
Size: 54 1425 50 275 18 1597 889 1629
Cuts at: 5945 8
Size: 43
Fragments arranged by size:
1629 1597 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3427 1537
Size: 275 1615 4090
Fragments arranged by size:
4090 1615 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2985 3138 3910 1353
Size: 1632 153 772 3423
Fragments arranged by size:
3423 1632 772 153
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2914 3236 4567 4941 5087 387
Size: 1064 138 1325 322 1331 374 146 1280
Fragments arranged by size:
1331 1325 1280 1064 374 322 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2369 2450 2603 2732 3070 3245 4043
Size: 393 737 81 153 129 338 175 798
Cuts at: 4043 4640 5427 5695 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 798 787 737 597 393 338 268
175 153 129 81
EcoNI CCTnn'n_nnAGG
Cuts at: 3167 3167
Size: 5980
EcoO109I rG'GnC_Cy
Cuts at: 2644 3238 2644
Size: 594 5386
EcoRI G'AATT_C
Cuts at: 3289 3667 3289
Size: 378 5602
FspI TGC'GCA
Cuts at: 2413 4486 2413
Size: 2073 3907
FspAI rTGC'GCAy
Cuts at: 2413 2413
Size: 5980
HaeII r_GCGC'y
Cuts at: 1678 2373 3039 3229 5127 1678
Size: 695 666 190 1898 2531
Fragments arranged by size:
2531 1898 695 666 190
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2597 2629 2930 2962 3137
Size: 32 135 32 1025 32 301 32 175
Cuts at: 3137 3169 4621 4653 4695 4727 1373
Size: 32 1452 32 42 32 2626
Fragments arranged by size:
2626 1452 1025 301 175 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3324 3570 669
Size: 988 281 1386 246 3079
Fragments arranged by size:
3079 1386 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5980
HpaI GTT'AAC
Cuts at: 3570 3570
Size: 5980
KpnI G_GTAC'C
Cuts at: 3305 3305
Size: 5980
MfeI C'AATT_G
Cuts at: 3579 3579
Size: 5980
MluI A'CGCG_T
Cuts at: 5856 5856
Size: 5980
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2479 5094 5278 1305
Size: 1174 2615 184 2007
Fragments arranged by size:
2615 2007 1174 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4158
Fragments arranged by size:
4158 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3938 4297 4456 518
Size: 648 2772 359 159 2042
Fragments arranged by size:
2772 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3396 4073 5221 5466 1606
Size: 1790 677 1148 245 2120
Fragments arranged by size:
2120 1790 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4489
Fragments arranged by size:
4489 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5980
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5980
NotI GC'GGCC_GC
Cuts at: 3427 3427
Size: 5980
NspI r_CATG'y
Cuts at: 1132 1229 2059 2209 3338 4883 1132
Size: 97 830 150 1129 1545 2229
Fragments arranged by size:
2229 1545 1129 830 150 97
PasI CC'CwG_GG
Cuts at: 1348 2045 1348
Size: 697 5283
PciI A'CATG_T
Cuts at: 4879 4879
Size: 5980
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5980
PfoI T'CCnGG_A
Cuts at: 2145 2187 2484 2145
Size: 42 297 5641
Fragments arranged by size:
5641 297 42
PmeI GTTT'AAAC
Cuts at: 3284 3284
Size: 5980
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4049 4081 5562 5594 4049
Size: 32 1481 32 4435
Fragments arranged by size:
4435 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3238 3238
Size: 5980
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5980
PsiI TTA'TAA
Cuts at: 3550 3550
Size: 5980
PspXI vC'TCGA_Gb
Cuts at: 2074 2074
Size: 5980
PstI C_TGCA'G
Cuts at: 830 3332 830
Size: 2502 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5980
SalI G'TCGA_C
Cuts at: 1936 3322 1936
Size: 1386 4594
SanDI GG'GwC_CC
Cuts at: 3238 3238
Size: 5980
SbfI CC_TGCA'GG
Cuts at: 3332 3332
Size: 5980
ScaI AGT'ACT
Cuts at: 4228 5940 4228
Size: 1712 4268
SfcI C'TryA_G
Cuts at: 826 1045 2796 3328 4463 5144 5335 826
Size: 219 1751 532 1135 681 191 1471
Fragments arranged by size:
1751 1471 1135 681 532 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5980
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5980
SmaI CCC'GGG
Cuts at: 1872 3307 1872
Size: 1435 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2074 2729 4084 4985 5247 820
Size: 197 926 131 655 1355 901 262 1553
Fragments arranged by size:
1553 1355 926 901 655 262 197 131
SnaBI TAC'GTA
Cuts at: 493 3771 493
Size: 3278 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5980
SphI G_CATG'C
Cuts at: 1132 3338 1132
Size: 2206 3774
SspI AAT'ATT
Cuts at: 5 52 3904 5
Size: 47 3852 2081
Fragments arranged by size:
3852 2081 47
StuI AGG'CCT
Cuts at: 1517 2318 1517
Size: 801 5179
StyI C'CwwG_G
Cuts at: 513 1065 2004 2122 3419 513
Size: 552 939 118 1297 3074
Fragments arranged by size:
3074 1297 939 552 118
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4007 4024 4177 4362 4807 325
Size: 1255 2427 17 153 185 445 1498
Fragments arranged by size:
2427 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4226 5938 96
Size: 274 80 33 51 1443 2249 1712 138
Fragments arranged by size:
2249 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2393 2425 3260 3292 5940 5972 2393
Size: 32 835 32 2648 32 2401
Fragments arranged by size:
2648 2401 835 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2944 1316
Size: 1628 4352
XbaI T'CTAG_A
Cuts at: 3316 3316
Size: 5980
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3040 3040
Size: 5980
XhoI C'TCGA_G
Cuts at: 1943 2074 1943
Size: 131 5849
XmaI C'CCGG_G
Cuts at: 1870 3305 1870
Size: 1435 4545
XmnI GAAnn'nnTTC
Cuts at: 4109 4109
Size: 5980
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3788 276
Size: 53 83 186 936 2254 2468
Fragments arranged by size:
2468 2254 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspXI PstI PvuI SalI SanDI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I EcoICRI EcoRV FalI
FseI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspOMI PsrI PvuII RsrII SacI SacII SapI
SexAI SfiI SfoI SrfI SwaI