Restriction map for
pFN21AE1421
(Circular) (Six-base) MAPSORT of: pFN21AE1421.seq Check: 8821 from: 1 to: 6151
pFN21AE1421 6151 bp
With 182 enzymes: SgfI *
July 6, 2012 17:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2404 2404
Size: 6151
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3961 278
Size: 53 83 186 936 2425 2468
Fragments arranged by size:
2468 2425 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3279 3494 1937
Size: 1342 215 4594
Fragments arranged by size:
4594 1342 215
Acc65I G'GTAC_C
Cuts at: 3472 3472
Size: 6151
AclI AA'CG_TT
Cuts at: 1563 4278 4651 1563
Size: 2715 373 3063
Fragments arranged by size:
3063 2715 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4214 5598 5866 1632
Size: 2582 1384 268 1917
Fragments arranged by size:
2582 1917 1384 268
AfeI AGC'GCT
Cuts at: 2811 2811
Size: 6151
AflII C'TTAA_G
Cuts at: 820 1017 2908 820
Size: 197 1891 4063
Fragments arranged by size:
4063 1891 197
AflIII A'CryG_T
Cuts at: 1203 5050 6027 1203
Size: 3847 977 1327
Fragments arranged by size:
3847 1327 977
AgeI A'CCGG_T
Cuts at: 4982 4982
Size: 6151
AhdI GACnn_n'nnGTC
Cuts at: 4880 4880
Size: 6151
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6151
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2431 1184
Size: 1247 4904
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2693 2725 2693
Size: 32 6119
AlwNI CAG_nnn'CTG
Cuts at: 2287 2308 3118 3295 3894 5466 2287
Size: 21 810 177 599 1572 2972
Fragments arranged by size:
2972 1572 810 599 177 21
ApaI G_GGCC'C
Cuts at: 2038 2038
Size: 6151
ApaLI G'TGCA_C
Cuts at: 3323 4208 5364 3323
Size: 885 1156 4110
Fragments arranged by size:
4110 1156 885
ApoI r'AATT_y
Cuts at: 1493 3460 3686 3838 5947 1493
Size: 1967 226 152 2109 1697
Fragments arranged by size:
2109 1967 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4705 160
Size: 872 3673 1606
Fragments arranged by size:
3673 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6151
AvaI C'yCGr_G
Cuts at: 1870 1943 2023 2748 2930 2988 3075 3088 3129
Size: 73 80 725 182 58 87 13 41
Cuts at: 3129 3215 3476 1870
Size: 86 261 4545
Fragments arranged by size:
4545 725 261 182 87 86 80 73
58 41 13
AvrII C'CTAG_G
Cuts at: 2788 2788
Size: 6151
BamHI G'GATC_C
Cuts at: 3061 3481 3848 3061
Size: 420 367 5364
Fragments arranged by size:
5364 420 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2328 2422 2462 2936 3151
Size: 325 217 627 541 94 40 474 215
Cuts at: 3151 3164 3472 4927 618
Size: 13 308 1455 1842
Fragments arranged by size:
1842 1455 627 541 474 325 308 217
215 94 40 13
BanII G_rGCy'C
Cuts at: 1391 2038 2103 2513 3082 3216 3432 3470 1391
Size: 647 65 410 569 134 216 38 4072
Fragments arranged by size:
4072 647 569 410 216 134 65 38
BbeI G_GCGC'C
Cuts at: 2332 2940 3155 2332
Size: 608 215 5328
Fragments arranged by size:
5328 608 215
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2719 928
Size: 971 820 4360
Fragments arranged by size:
4360 971 820
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3066 3100 4342 4376 1903
Size: 34 1129 34 1242 34 3678
Fragments arranged by size:
3678 1242 1129 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4043 5253 4043
Size: 1210 4941
BclI T'GATC_A
Cuts at: 1548 2430 1548
Size: 882 5269
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2405 2993 4762
Size: 107 122 71 631 605 733 588 1769
Cuts at: 4762 136
Size: 1525
Fragments arranged by size:
1769 1525 733 631 605 588 122 107
71
BglII A'GATC_T
Cuts at: 6146 6146
Size: 6151
BlpI GC'TnA_GC
Cuts at: 2951 3344 3568 2951
Size: 393 224 5534
Fragments arranged by size:
5534 393 224
Bme1580I G_kGCm'C
Cuts at: 1790 2038 2454 3327 4212 5368 1790
Size: 248 416 873 885 1156 2573
Fragments arranged by size:
2573 1156 885 873 416 248
BmgBI CAC'GTC
Cuts at: 1318 2441 3283 1318
Size: 1123 842 4186
Fragments arranged by size:
4186 1123 842
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3882 4840 442
Size: 416 53 83 399 24 2465 958 1753
Fragments arranged by size:
2465 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6151
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2284 2284
Size: 6151
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2279 2330 2498 2879 4811 1239
Size: 1040 51 168 381 1932 2579
Fragments arranged by size:
2579 1932 1040 381 168 51
Bpu10I CC'TnA_GC
Cuts at: 2150 2976 2150
Size: 826 5325
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4276 5141 5439 4276
Size: 865 298 4988
Fragments arranged by size:
4988 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2258 4814 882
Size: 617 759 2556 2219
Fragments arranged by size:
2556 2219 759 617
BsaAI yAC'GTr
Cuts at: 493 1204 3942 493
Size: 711 2738 2702
Fragments arranged by size:
2738 2702 711
BsaBI GATnn'nnATC
Cuts at: 2234 6145 2234
Size: 3911 2240
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2329 2937 3152 3958
Size: 53 83 186 936 796 608 215 806
Cuts at: 3958 4340 4975 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 806 796 635 608 382 215
186 83 53
BsaWI w'CCGG_w
Cuts at: 4584 4982 5256 5403 5814 4584
Size: 398 274 147 411 4921
Fragments arranged by size:
4921 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2669 2699 1178
Size: 30 165 30 1266 30 4630
Fragments arranged by size:
4630 1266 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2276 2882 3155 1186
Size: 1090 606 273 4182
Fragments arranged by size:
4182 1090 606 273
BseYI C'CCAG_C
Cuts at: 3236 5354 5850 3236
Size: 2118 496 3537
Fragments arranged by size:
3537 2118 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3191 1163
Size: 573 1455 4123
Fragments arranged by size:
4123 1455 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3394 3601 4362 5390 1540
Size: 275 186 1393 207 761 1028 2301
Fragments arranged by size:
2301 1393 1028 761 275 207 186
BsiHKAI G_wGCw'C
Cuts at: 3089 3216 3327 3432 4212 4297 5368 3089
Size: 127 111 105 780 85 1071 3872
Fragments arranged by size:
3872 1071 780 127 111 105 85
BsmI GAATG_Cn'
Cuts at: 2350 3662 3755 2350
Size: 1312 93 4746
Fragments arranged by size:
4746 1312 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2130 2130
Size: 6151
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2038 2103 2454 2513 3082 3089 3216
Size: 399 248 65 351 59 569 7 127
Cuts at: 3216 3327 3432 3470 4212 4297 5368 1391
Size: 111 105 38 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 569 399 351 248 127
111 105 85 65 59 38 7
BspEI T'CCGG_A
Cuts at: 5814 5814
Size: 6151
BspHI T'CATG_A
Cuts at: 4040 5770 4040
Size: 1730 4421
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2404 3506 844
Size: 1560 1102 3489
Fragments arranged by size:
3489 1560 1102
BsrBI CCG'CTC
Cuts at: 1394 1823 2690 3037 3247 4038 1394
Size: 429 867 347 210 791 3507
Fragments arranged by size:
3507 867 791 429 347 210
BsrDI GCAATG_nn'
Cuts at: 65 1248 4646 4820 65
Size: 1183 3398 174 1396
Fragments arranged by size:
3398 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3108 4795 4982 1700
Size: 110 1298 1687 187 2869
Fragments arranged by size:
2869 1687 1298 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6151
BssHII G'CGCG_C
Cuts at: 1925 2199 3396 1925
Size: 274 1197 4680
Fragments arranged by size:
4680 1197 274
BssSI C'ACGA_G
Cuts at: 2672 3209 4211 5223 2672
Size: 537 1002 1012 3600
Fragments arranged by size:
3600 1012 1002 537
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2530 3118 794
Size: 1736 588 3827
Fragments arranged by size:
3827 1736 588
BstBI TT'CG_AA
Cuts at: 4955 4955
Size: 6151
BstEII G'GTnAC_C
Cuts at: 1184 2920 1184
Size: 1736 4415
BstXI CCAn_nnnn'nTGG
Cuts at: 2230 2325 2230
Size: 95 6056
BstYI r'GATC_y
Cuts at: 1972 2254 3061 3481 3848 4233 4250 5702 5788
Size: 282 807 420 367 385 17 1452 86
Cuts at: 5788 5800 6146 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 807 420 385 367 346 282
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2625 2820 2997 3168 3301 513
Size: 552 939 621 195 177 171 133 3363
Fragments arranged by size:
3363 939 621 552 195 177 171 133
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5412
Fragments arranged by size:
5412 683 56
BtsI GCAGTG_nn'
Cuts at: 3667 4461 4481 3667
Size: 794 20 5337
Fragments arranged by size:
5337 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6151
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6116
DraI TTT'AAA
Cuts at: 3455 3455
Size: 6151
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5158 809
Size: 507 3842 1802
Fragments arranged by size:
3842 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2178 2232 2315
Size: 54 1425 50 275 18 348 54 83
Cuts at: 2315 2994 3025 3598 4487 6116 8
Size: 679 31 573 889 1629 43
Fragments arranged by size:
1629 1425 889 679 573 348 275 83
54 54 50 43 31 18
EagI C'GGCC_G
Cuts at: 1537 1812 3598 1537
Size: 275 1786 4090
Fragments arranged by size:
4090 1786 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4081 1353
Size: 2728 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3239 4738 5112 5258 387
Size: 1064 138 1650 1499 374 146 1280
Fragments arranged by size:
1650 1499 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3214 3430 3214
Size: 216 5935
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2279 2330 2498 2879 4214 4811 5598
Size: 393 647 51 168 381 1335 597 787
Cuts at: 5598 5866 1239
Size: 268 1524
Fragments arranged by size:
1524 1335 787 647 597 393 381 268
168 51
EcoNI CCTnn'n_nnAGG
Cuts at: 3371 3371
Size: 6151
EcoO109I rG'GnC_Cy
Cuts at: 2034 2035 2284 2914 3306 2034
Size: 1 249 630 392 4879
Fragments arranged by size:
4879 630 392 249 1
EcoRI G'AATT_C
Cuts at: 3460 3838 3460
Size: 378 5773
FspI TGC'GCA
Cuts at: 4657 4657
Size: 6151
HaeII r_GCGC'y
Cuts at: 1678 2332 2813 2876 2940 2985 3005 3155 3188
Size: 654 481 63 64 45 20 150 33
Cuts at: 3188 3200 3380 5298 1678
Size: 12 180 1918 2531
Fragments arranged by size:
2531 1918 654 481 180 150 64 63
45 33 20 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2484 2516 2669 2701 2959
Size: 32 135 32 912 32 153 32 258
Cuts at: 2959 2991 2999 3031 4792 4824 4866 4898 1373
Size: 32 8 32 1761 32 42 32 2626
Fragments arranged by size:
2626 1761 912 258 153 135 42 32
32 32 32 32 32 32 32 8
HincII GTy'rAC
Cuts at: 669 1657 1938 3280 3495 3741 669
Size: 988 281 1342 215 246 3079
Fragments arranged by size:
3079 1342 988 281 246 215
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6151
HpaI GTT'AAC
Cuts at: 3741 3741
Size: 6151
KasI G'GCGC_C
Cuts at: 2328 2936 3151 2328
Size: 608 215 5328
Fragments arranged by size:
5328 608 215
KpnI G_GTAC'C
Cuts at: 3476 3476
Size: 6151
MfeI C'AATT_G
Cuts at: 3750 3750
Size: 6151
MluI A'CGCG_T
Cuts at: 6027 6027
Size: 6151
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2263 3414 5265 5449 1305
Size: 958 1151 1851 184 2007
Fragments arranged by size:
2007 1851 1151 958 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2234 2317 10
Size: 54 1425 343 402 83 3844
Fragments arranged by size:
3844 1425 402 343 83 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2228 4109 4468 4627 518
Size: 648 1062 1881 359 159 2042
Fragments arranged by size:
2042 1881 1062 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2183 2308 2436 2575 2955 2999 3115 3193
Size: 577 125 128 139 380 44 116 78
Cuts at: 3193 3348 3567 4244 5392 5637 1606
Size: 155 219 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 577 380 245 219 155
139 128 125 116 78 44
NaeI GCC'GGC
Cuts at: 3110 3110
Size: 6151
NarI GG'CG_CC
Cuts at: 2329 2937 3152 2329
Size: 608 215 5328
Fragments arranged by size:
5328 608 215
NcoI C'CATG_G
Cuts at: 513 1065 2004 2820 513
Size: 552 939 816 3844
Fragments arranged by size:
3844 939 816 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6151
NgoMIV G'CCGG_C
Cuts at: 3108 3108
Size: 6151
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6151
NotI GC'GGCC_GC
Cuts at: 3598 3598
Size: 6151
NspI r_CATG'y
Cuts at: 1132 1229 2412 3509 5054 1132
Size: 97 1183 1097 1545 2229
Fragments arranged by size:
2229 1545 1183 1097 97
PasI CC'CwG_GG
Cuts at: 1348 2833 1348
Size: 1485 4666
PciI A'CATG_T
Cuts at: 5050 5050
Size: 6151
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2287 2857 1108
Size: 1179 570 4402
Fragments arranged by size:
4402 1179 570
PfoI T'CCnGG_A
Cuts at: 2112 2488 2112
Size: 376 5775
PmeI GTTT'AAAC
Cuts at: 3455 3455
Size: 6151
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4220 4252 5733 5765 4220
Size: 32 1481 32 4606
Fragments arranged by size:
4606 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2914 3306 2914
Size: 392 5759
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6151
PsiI TTA'TAA
Cuts at: 3721 3721
Size: 6151
PspOMI G'GGCC_C
Cuts at: 2034 2034
Size: 6151
PstI C_TGCA'G
Cuts at: 830 3372 3503 830
Size: 2542 131 3478
Fragments arranged by size:
3478 2542 131
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6151
PvuII CAG'CTG
Cuts at: 2308 2575 3115 2308
Size: 267 540 5344
Fragments arranged by size:
5344 540 267
SacI G_AGCT'C
Cuts at: 3216 3432 3216
Size: 216 5935
SacII CC_GC'GG
Cuts at: 3000 3000
Size: 6151
SalI G'TCGA_C
Cuts at: 1936 3278 3493 1936
Size: 1342 215 4594
Fragments arranged by size:
4594 1342 215
SanDI GG'GwC_CC
Cuts at: 3306 3306
Size: 6151
SbfI CC_TGCA'GG
Cuts at: 3372 3503 3372
Size: 131 6020
ScaI AGT'ACT
Cuts at: 4399 6111 4399
Size: 1712 4439
SexAI A'CCwGG_T
Cuts at: 2916 2916
Size: 6151
SfcI C'TryA_G
Cuts at: 826 1045 3368 3499 4634 5315 5506 826
Size: 219 2323 131 1135 681 191 1471
Fragments arranged by size:
2323 1471 1135 681 219 191 131
SfoI GGC'GCC
Cuts at: 2330 2938 3153 2330
Size: 608 215 5328
Fragments arranged by size:
5328 608 215
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6151
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6151
SmaI CCC'GGG
Cuts at: 1872 2025 2750 2990 3131 3478 1872
Size: 153 725 240 141 347 4545
Fragments arranged by size:
4545 725 347 240 153 141
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2908 4255 5156 5418 820
Size: 197 926 965 1347 901 262 1553
Fragments arranged by size:
1553 1347 965 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3942 493
Size: 3449 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6151
SphI G_CATG'C
Cuts at: 1132 3509 1132
Size: 2377 3774
SspI AAT'ATT
Cuts at: 5 52 4075 5
Size: 47 4023 2081
Fragments arranged by size:
4023 2081 47
StuI AGG'CCT
Cuts at: 1517 2324 1517
Size: 807 5344
StyI C'CwwG_G
Cuts at: 513 1065 2004 2466 2788 2820 3022 3590 513
Size: 552 939 462 322 32 202 568 3074
Fragments arranged by size:
3074 939 568 552 462 322 202 32
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2357 4178 4195 4348 4533 4978 325
Size: 1255 777 1821 17 153 185 445 1498
Fragments arranged by size:
1821 1498 1255 777 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4397 6109 96
Size: 274 80 33 51 1443 2420 1712 138
Fragments arranged by size:
2420 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6111 6143 6111
Size: 32 6119
Tth111I GACn'n_nGTC
Cuts at: 1316 2375 2442 2918 1316
Size: 1059 67 476 4549
Fragments arranged by size:
4549 1059 476 67
XbaI T'CTAG_A
Cuts at: 3487 3487
Size: 6151
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2362 2362
Size: 6151
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6151
XmaI C'CCGG_G
Cuts at: 1870 2023 2748 2988 3129 3476 1870
Size: 153 725 240 141 347 4545
Fragments arranged by size:
4545 725 347 240 153 141
XmnI GAAnn'nnTTC
Cuts at: 4280 4280
Size: 6151
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3959 276
Size: 53 83 186 936 2425 2468
Fragments arranged by size:
2468 2425 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SacII SalI SanDI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI BaeI BbvCI BfrBI BsiWI BstZ17I Bsu36I DraIII
EcoRV FalI FseI FspAI NruI NsiI PacI PmlI
PspXI PsrI RsrII SapI SfiI SrfI SwaI