Restriction map for
pFN21AE1418
(Circular) (Six-base) MAPSORT of: pFN21AE1418.seq Check: 7014 from: 1 to: 5932
pFN21AE1418 5932 bp
With 182 enzymes: SgfI *
August 16, 2010 14:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3742 278
Size: 53 83 186 936 2206 2468
Fragments arranged by size:
2468 2206 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3275 1937
Size: 1338 4594
Acc65I G'GTAC_C
Cuts at: 3253 3253
Size: 5932
AclI AA'CG_TT
Cuts at: 1563 4059 4432 1563
Size: 2496 373 3063
Fragments arranged by size:
3063 2496 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2218 2240 2347 3995 5379 5647 1632
Size: 586 22 107 1648 1384 268 1917
Fragments arranged by size:
1917 1648 1384 586 268 107 22
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5735
AflIII A'CryG_T
Cuts at: 1203 2176 2730 2984 3069 4831 5808 1203
Size: 973 554 254 85 1762 977 1327
Fragments arranged by size:
1762 1327 977 973 554 254 85
AgeI A'CCGG_T
Cuts at: 4763 4763
Size: 5932
AhdI GACnn_n'nnGTC
Cuts at: 4661 4661
Size: 5932
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5932
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3108 1184
Size: 1924 4008
AlwNI CAG_nnn'CTG
Cuts at: 3675 5247 3675
Size: 1572 4360
ApaLI G'TGCA_C
Cuts at: 3989 5145 3989
Size: 1156 4776
ApoI r'AATT_y
Cuts at: 1493 2324 3241 3467 3619 5728 1493
Size: 831 917 226 152 2109 1697
Fragments arranged by size:
2109 1697 917 831 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4486 160
Size: 872 3454 1606
Fragments arranged by size:
3454 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5932
AvaI C'yCGr_G
Cuts at: 1870 1943 2102 3257 1870
Size: 73 159 1155 4545
Fragments arranged by size:
4545 1155 159 73
BamHI G'GATC_C
Cuts at: 2973 3262 3629 2973
Size: 289 367 5276
Fragments arranged by size:
5276 367 289
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3059 3253 4708 618
Size: 325 217 627 1272 194 1455 1842
Fragments arranged by size:
1842 1455 1272 627 325 217 194
BanII G_rGCy'C
Cuts at: 1391 2417 3251 1391
Size: 1026 834 4072
Fragments arranged by size:
4072 1026 834
BbeI G_GCGC'C
Cuts at: 3063 3063
Size: 5932
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4961
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4123 4157 1903
Size: 34 2186 34 3678
Fragments arranged by size:
3678 2186 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2927 3824 5034 2927
Size: 897 1210 3825
Fragments arranged by size:
3825 1210 897
BclI T'GATC_A
Cuts at: 1548 2430 1548
Size: 882 5050
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2537 3036 3086
Size: 107 122 71 631 605 865 499 50
Cuts at: 3086 4543 136
Size: 1457 1525
Fragments arranged by size:
1525 1457 865 631 605 499 122 107
71 50
BglII A'GATC_T
Cuts at: 5927 5927
Size: 5932
BlpI GC'TnA_GC
Cuts at: 3349 3349
Size: 5932
Bme1580I G_kGCm'C
Cuts at: 1790 2288 2858 3993 5149 1790
Size: 498 570 1135 1156 2573
Fragments arranged by size:
2573 1156 1135 570 498
BmgBI CAC'GTC
Cuts at: 1318 3072 1318
Size: 1754 4178
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3107 3663 4621
Size: 416 53 83 399 24 1690 556 958
Cuts at: 4621 442
Size: 1753
Fragments arranged by size:
1753 1690 958 556 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5932
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2819 2819
Size: 5932
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2261 2721 2761 2859 4592 1239
Size: 1022 460 40 98 1733 2579
Fragments arranged by size:
2579 1733 1022 460 98 40
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4057 4922 5220 4057
Size: 865 298 4769
Fragments arranged by size:
4769 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2926 4595 882
Size: 617 1427 1669 2219
Fragments arranged by size:
2219 1669 1427 617
BsaAI yAC'GTr
Cuts at: 493 1204 2733 3723 493
Size: 711 1529 990 2702
Fragments arranged by size:
2702 1529 990 711
BsaBI GATnn'nnATC
Cuts at: 5926 5926
Size: 5932
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3060 3739 4121 4756
Size: 53 83 186 936 1527 679 382 635
Cuts at: 4756 275
Size: 1451
Fragments arranged by size:
1527 1451 936 679 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4365 4763 5037 5184 5595 4365
Size: 398 274 147 411 4702
Fragments arranged by size:
4702 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2835 2865 1178
Size: 30 165 30 1432 30 4245
Fragments arranged by size:
4245 1432 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2856 1186
Size: 1670 4262
BseYI C'CCAG_C
Cuts at: 2672 5135 5631 2672
Size: 2463 496 2973
Fragments arranged by size:
2973 2463 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2388 2734 3078 1163
Size: 573 652 346 344 4017
Fragments arranged by size:
4017 652 573 346 344
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3382 4143 5171 1540
Size: 275 186 1381 761 1028 2301
Fragments arranged by size:
2301 1381 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2760 2793 3993 4078 5149 2760
Size: 33 1200 85 1071 3543
Fragments arranged by size:
3543 1200 1071 85 33
BsmI GAATG_Cn'
Cuts at: 2830 3443 3536 2830
Size: 613 93 5226
Fragments arranged by size:
5226 613 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2288 2417 2760 2793 2858 3251 3993
Size: 399 498 129 343 33 65 393 742
Cuts at: 3993 4078 5149 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 498 399 393 343 129
85 65 33
BspEI T'CCGG_A
Cuts at: 5595 5595
Size: 5932
BspHI T'CATG_A
Cuts at: 2208 2433 3821 5551 2208
Size: 225 1388 1730 2589
Fragments arranged by size:
2589 1730 1388 225
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3287 844
Size: 2443 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3819 1394
Size: 429 1996 3507
Fragments arranged by size:
3507 1996 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2643 2688 2907 3060 4427 4601 65
Size: 1183 1395 45 219 153 1367 174 1396
Fragments arranged by size:
1396 1395 1367 1183 219 174 153 45
BsrFI r'CCGG_y
Cuts at: 1700 1810 4576 4763 1700
Size: 110 2766 187 2869
Fragments arranged by size:
2869 2766 187 110
BsrGI T'GTAC_A
Cuts at: 96 2173 2293 96
Size: 2077 120 3735
Fragments arranged by size:
3735 2077 120
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5932
BssSI C'ACGA_G
Cuts at: 3992 5004 3992
Size: 1012 4920
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5932
BstBI TT'CG_AA
Cuts at: 4736 4736
Size: 5932
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5932
BstXI CCAn_nnnn'nTGG
Cuts at: 2534 2785 2534
Size: 251 5681
BstYI r'GATC_y
Cuts at: 1972 2973 3262 3629 4014 4031 5483 5569 5581
Size: 1001 289 367 385 17 1452 86 12
Cuts at: 5581 5927 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1001 385 367 346 289 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 3004 513
Size: 552 1939 3441
Fragments arranged by size:
3441 1939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5193
Fragments arranged by size:
5193 683 56
BtsI GCAGTG_nn'
Cuts at: 2245 3448 4242 4262 2245
Size: 1203 794 20 3915
Fragments arranged by size:
3915 1203 794 20
ClaI AT'CG_AT
Cuts at: 1554 2575 1554
Size: 1021 4911
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5897
DraI TTT'AAA
Cuts at: 3236 3236
Size: 5932
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4939 809
Size: 507 3623 1802
Fragments arranged by size:
3623 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2685 3078 3379
Size: 54 1425 50 275 18 855 393 301
Cuts at: 3379 4268 5897 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 855 393 301 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3379 1537
Size: 275 1567 4090
Fragments arranged by size:
4090 1567 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2601 3862 1353
Size: 1248 1261 3423
Fragments arranged by size:
3423 1261 1248
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4519 4893 5039 387
Size: 1064 138 2930 374 146 1280
Fragments arranged by size:
2930 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2218 2240 2261 2347 2721 2761 2859
Size: 393 586 22 21 86 374 40 98
Cuts at: 2859 3995 4592 5379 5647 1239
Size: 1136 597 787 268 1524
Fragments arranged by size:
1524 1136 787 597 586 393 374 268
98 86 40 22 21
EcoO109I rG'GnC_Cy
Cuts at: 2538 3217 2538
Size: 679 5253
EcoRI G'AATT_C
Cuts at: 3241 3619 3241
Size: 378 5554
EcoRV GAT'ATC
Cuts at: 2385 2385
Size: 5932
FspI TGC'GCA
Cuts at: 4438 4438
Size: 5932
HaeII r_GCGC'y
Cuts at: 1678 3063 5079 1678
Size: 1385 2016 2531
Fragments arranged by size:
2531 2016 1385
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2632 2664 4573 4605 4647
Size: 32 135 32 1060 32 1909 32 42
Cuts at: 4647 4679 1373
Size: 32 2626
Fragments arranged by size:
2626 1909 1060 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3276 3522 669
Size: 988 281 1338 246 3079
Fragments arranged by size:
3079 1338 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5932
HpaI GTT'AAC
Cuts at: 3522 3522
Size: 5932
KasI G'GCGC_C
Cuts at: 3059 3059
Size: 5932
KpnI G_GTAC'C
Cuts at: 3257 3257
Size: 5932
MfeI C'AATT_G
Cuts at: 3531 3531
Size: 5932
MluI A'CGCG_T
Cuts at: 5808 5808
Size: 5932
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3090 3106 5046 5230 1305
Size: 1785 16 1940 184 2007
Fragments arranged by size:
2007 1940 1785 184 16
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3080 10
Size: 54 1425 343 1248 2862
Fragments arranged by size:
2862 1425 1248 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2423 3074 3890 4249 4408 518
Size: 648 1257 651 816 359 159 2042
Fragments arranged by size:
2042 1257 816 651 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3092 3348 4025 5173 5418 1606
Size: 1486 256 677 1148 245 2120
Fragments arranged by size:
2120 1486 1148 677 256 245
NarI GG'CG_CC
Cuts at: 3060 3060
Size: 5932
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5380
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5932
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5932
NotI GC'GGCC_GC
Cuts at: 3379 3379
Size: 5932
NspI r_CATG'y
Cuts at: 1132 1229 2180 2988 3290 4835 1132
Size: 97 951 808 302 1545 2229
Fragments arranged by size:
2229 1545 951 808 302 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5932
PciI A'CATG_T
Cuts at: 2176 2984 4831 2176
Size: 808 1847 3277
Fragments arranged by size:
3277 1847 808
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5932
PfoI T'CCnGG_A
Cuts at: 2037 2037
Size: 5932
PmeI GTTT'AAAC
Cuts at: 3236 3236
Size: 5932
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4001 4033 5514 5546 4001
Size: 32 1481 32 4387
Fragments arranged by size:
4387 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3217 3217
Size: 5932
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5932
PsiI TTA'TAA
Cuts at: 3502 3502
Size: 5932
PstI C_TGCA'G
Cuts at: 830 3284 830
Size: 2454 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5932
PvuII CAG'CTG
Cuts at: 3092 3092
Size: 5932
SalI G'TCGA_C
Cuts at: 1936 3274 1936
Size: 1338 4594
SbfI CC_TGCA'GG
Cuts at: 3284 3284
Size: 5932
ScaI AGT'ACT
Cuts at: 4180 5892 4180
Size: 1712 4220
SexAI A'CCwGG_T
Cuts at: 2475 3013 2475
Size: 538 5394
SfcI C'TryA_G
Cuts at: 826 1045 2031 2556 3280 4415 5096 5287 826
Size: 219 986 525 724 1135 681 191 1471
Fragments arranged by size:
1471 1135 986 724 681 525 219 191
SfoI GGC'GCC
Cuts at: 3061 3061
Size: 5932
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5932
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5932
SmaI CCC'GGG
Cuts at: 1872 3259 1872
Size: 1387 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2102 4036 4937 5199 820
Size: 197 926 159 1934 901 262 1553
Fragments arranged by size:
1934 1553 926 901 262 197 159
SnaBI TAC'GTA
Cuts at: 493 3723 493
Size: 3230 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5932
SphI G_CATG'C
Cuts at: 1132 3290 1132
Size: 2158 3774
SspI AAT'ATT
Cuts at: 5 52 2490 3856 5
Size: 47 2438 1366 2081
Fragments arranged by size:
2438 2081 1366 47
StuI AGG'CCT
Cuts at: 1517 2498 3030 1517
Size: 981 532 4419
Fragments arranged by size:
4419 981 532
StyI C'CwwG_G
Cuts at: 513 1065 3371 513
Size: 552 2306 3074
Fragments arranged by size:
3074 2306 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2435 2895 3959 3976 4129 4314 4759
Size: 1255 855 460 1064 17 153 185 445
Cuts at: 4759 325
Size: 1498
Fragments arranged by size:
1498 1255 1064 855 460 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2173 2293 4178
Size: 274 80 33 51 1443 196 120 1885
Cuts at: 4178 5890 96
Size: 1712 138
Fragments arranged by size:
1885 1712 1443 274 196 138 120 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2835 2867 5892 5924 2835
Size: 32 3025 32 2843
Fragments arranged by size:
3025 2843 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5932
XbaI T'CTAG_A
Cuts at: 3268 3268
Size: 5932
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2984 3011 3073 2984
Size: 27 62 5843
Fragments arranged by size:
5843 62 27
XhoI C'TCGA_G
Cuts at: 1943 2102 1943
Size: 159 5773
XmaI C'CCGG_G
Cuts at: 1870 3257 1870
Size: 1387 4545
XmnI GAAnn'nnTTC
Cuts at: 4061 4061
Size: 5932
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3740 276
Size: 53 83 186 936 2206 2468
Fragments arranged by size:
2468 2206 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoNI FalI FseI FspAI NaeI NgoMIV NruI NsiI
PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SapI SfiI SrfI SwaI