Restriction map for
pFN21AE1417
(Circular) (Six-base) MAPSORT of: pFN21AE1417.seq Check: 2615 from: 1 to: 6463
pFN21AE1417 6463 bp
With 182 enzymes: SgfI *
August 16, 2010 14:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4273 278
Size: 53 83 186 936 2737 2468
Fragments arranged by size:
2737 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3806 1937
Size: 1869 4594
Acc65I G'GTAC_C
Cuts at: 2439 3784 2439
Size: 1345 5118
AclI AA'CG_TT
Cuts at: 1563 4590 4963 1563
Size: 3027 373 3063
Fragments arranged by size:
3063 3027 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2201 2283 2600 4526 5910 6178 1632
Size: 569 82 317 1926 1384 268 1917
Fragments arranged by size:
1926 1917 1384 569 317 268 82
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6266
AflIII A'CryG_T
Cuts at: 1203 5362 6339 1203
Size: 4159 977 1327
Fragments arranged by size:
4159 1327 977
AgeI A'CCGG_T
Cuts at: 5294 5294
Size: 6463
AhdI GACnn_n'nnGTC
Cuts at: 5192 5192
Size: 6463
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6463
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3273 1184
Size: 2089 4374
AlwNI CAG_nnn'CTG
Cuts at: 2596 3598 4206 5778 2596
Size: 1002 608 1572 3281
Fragments arranged by size:
3281 1572 1002 608
ApaI G_GGCC'C
Cuts at: 3121 3121
Size: 6463
ApaLI G'TGCA_C
Cuts at: 2426 4520 5676 2426
Size: 2094 1156 3213
Fragments arranged by size:
3213 2094 1156
ApoI r'AATT_y
Cuts at: 1493 3772 3998 4150 6259 1493
Size: 2279 226 152 2109 1697
Fragments arranged by size:
2279 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5017 160
Size: 872 3985 1606
Fragments arranged by size:
3985 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6463
AvaI C'yCGr_G
Cuts at: 1870 1943 2931 3627 3788 1870
Size: 73 988 696 161 4545
Fragments arranged by size:
4545 988 696 161 73
BamHI G'GATC_C
Cuts at: 2517 2841 3793 4160 2517
Size: 324 952 367 4820
Fragments arranged by size:
4820 952 367 324
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2439 2794 3784 5239 618
Size: 325 217 627 652 355 990 1455 1842
Fragments arranged by size:
1842 1455 990 652 627 355 325 217
BanII G_rGCy'C
Cuts at: 1391 2283 3099 3121 3492 3782 1391
Size: 892 816 22 371 290 4072
Fragments arranged by size:
4072 892 816 371 290 22
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3080 3383 928
Size: 971 1181 303 4008
Fragments arranged by size:
4008 1181 971 303
BbvCI CC'TCA_GC
Cuts at: 2274 2444 2274
Size: 170 6293
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4654 4688 1903
Size: 34 50 34 2633 34 3678
Fragments arranged by size:
3678 2633 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4355 5565 4355
Size: 1210 5253
BclI T'GATC_A
Cuts at: 1548 2095 1548
Size: 547 5916
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5074 136
Size: 107 122 71 631 605 3402 1525
Fragments arranged by size:
3402 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2346 6458 2346
Size: 4112 2351
BlpI GC'TnA_GC
Cuts at: 3605 3880 3605
Size: 275 6188
Bme1580I G_kGCm'C
Cuts at: 1790 2430 3121 3151 4524 5680 1790
Size: 640 691 30 1373 1156 2573
Fragments arranged by size:
2573 1373 1156 691 640 30
BmgBI CAC'GTC
Cuts at: 1318 2822 1318
Size: 1504 4959
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4194 5152 442
Size: 416 53 83 399 24 2777 958 1753
Fragments arranged by size:
2777 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6463
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2072 2216 2978 3149 3233 5123 1239
Size: 833 144 762 171 84 1890 2579
Fragments arranged by size:
2579 1890 833 762 171 144 84
Bpu10I CC'TnA_GC
Cuts at: 2274 2336 2444 2589 3201 2274
Size: 62 108 145 612 5536
Fragments arranged by size:
5536 612 145 108 62
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4588 5453 5751 4588
Size: 865 298 5300
Fragments arranged by size:
5300 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5126 882
Size: 617 3627 2219
Fragments arranged by size:
3627 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4254 493
Size: 711 3050 2702
Fragments arranged by size:
3050 2702 711
BsaBI GATnn'nnATC
Cuts at: 6457 6457
Size: 6463
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4270 4652 5287 275
Size: 53 83 186 936 2737 382 635 1451
Fragments arranged by size:
2737 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2514 4896 5294 5568 5715 6126 2514
Size: 2382 398 274 147 411 2851
Fragments arranged by size:
2851 2382 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6238
Fragments arranged by size:
6238 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2291 2951 3053 3083 3146 3149 3170 3221
Size: 1105 660 102 30 63 3 21 51
Cuts at: 3221 3401 3560 1186
Size: 180 159 4089
Fragments arranged by size:
4089 1105 660 180 159 102 63 51
30 21 3
BseYI C'CCAG_C
Cuts at: 5666 6162 5666
Size: 496 5967
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2414 2930 3174 3377 3605 1163
Size: 573 678 516 244 203 228 4021
Fragments arranged by size:
4021 678 573 516 244 228 203
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3913 4674 5702 1540
Size: 275 186 1912 761 1028 2301
Fragments arranged by size:
2301 1912 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2283 2376 2430 3099 4524 4609 5680 2283
Size: 93 54 669 1425 85 1071 3066
Fragments arranged by size:
3066 1425 1071 669 93 85 54
BsmI GAATG_Cn'
Cuts at: 3974 4067 3974
Size: 93 6370
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2283 2376 2430 3099 3121 3151 3492
Size: 399 493 93 54 669 22 30 341
Cuts at: 3492 3782 4524 4609 5680 1391
Size: 290 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 669 493 399 341 290
93 85 54 30 22
BspEI T'CCGG_A
Cuts at: 6126 6126
Size: 6463
BspHI T'CATG_A
Cuts at: 4352 6082 4352
Size: 1730 4733
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3818 844
Size: 2974 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2455 2962 4350 1394
Size: 429 632 507 1388 3507
Fragments arranged by size:
3507 1388 632 507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3547 4958 5132 65
Size: 1183 2299 1411 174 1396
Fragments arranged by size:
2299 1411 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3442 5107 5294 1700
Size: 110 1632 1665 187 2869
Fragments arranged by size:
2869 1665 1632 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6463
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6463
BssSI C'ACGA_G
Cuts at: 4523 5535 4523
Size: 1012 5451
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3025 794
Size: 2231 4232
BstBI TT'CG_AA
Cuts at: 5267 5267
Size: 6463
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6463
BstYI r'GATC_y
Cuts at: 1972 2346 2517 2841 3714 3793 4160 4545 4562
Size: 374 171 324 873 79 367 385 17
Cuts at: 4562 6014 6100 6112 6458 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 873 385 374 367 346 324
171 86 79 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2037 2542 3000 3492 513
Size: 552 972 505 458 492 3484
Fragments arranged by size:
3484 972 552 505 492 458
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2355 487
Size: 683 56 1129 4595
Fragments arranged by size:
4595 1129 683 56
BtsI GCAGTG_nn'
Cuts at: 3191 3276 3979 4773 4793 3191
Size: 85 703 794 20 4861
Fragments arranged by size:
4861 794 703 85 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6463
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6428
DraI TTT'AAA
Cuts at: 3767 3767
Size: 6463
DraIII CAC_nnn'GTG
Cuts at: 2152 3400 2152
Size: 1248 5215
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5470 809
Size: 507 4154 1802
Fragments arranged by size:
4154 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3910 4799 6428
Size: 54 1425 50 275 18 2080 889 1629
Cuts at: 6428 8
Size: 43
Fragments arranged by size:
2080 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3910 1537
Size: 275 2098 4090
Fragments arranged by size:
4090 2098 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3294 3327 4393 1353
Size: 1941 33 1066 3423
Fragments arranged by size:
3423 1941 1066 33
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5050 5424 5570 387
Size: 1064 138 3461 374 146 1280
Fragments arranged by size:
3461 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2281 3097 2281
Size: 816 5647
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2072 2201 2216 2283 2600 2978 3149
Size: 393 440 129 15 67 317 378 171
Cuts at: 3149 3233 4526 5123 5910 6178 1239
Size: 84 1293 597 787 268 1524
Fragments arranged by size:
1524 1293 787 597 440 393 378 317
268 171 129 84 67 15
EcoNI CCTnn'n_nnAGG
Cuts at: 3299 3299
Size: 6463
EcoO109I rG'GnC_Cy
Cuts at: 2382 2593 3117 3118 3645 3757 2382
Size: 211 524 1 527 112 5088
Fragments arranged by size:
5088 527 524 211 112 1
EcoRI G'AATT_C
Cuts at: 3772 4150 3772
Size: 378 6085
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2351 2737 2351
Size: 386 6077
FspI TGC'GCA
Cuts at: 4969 4969
Size: 6463
HaeII r_GCGC'y
Cuts at: 1678 3169 5610 1678
Size: 1491 2441 2531
Fragments arranged by size:
2531 2441 1491
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5104 5136 5178 5210 1373
Size: 32 135 32 3532 32 42 32 2626
Fragments arranged by size:
3532 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2706 3807 4053 669
Size: 988 281 768 1101 246 3079
Fragments arranged by size:
3079 1101 988 768 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6463
HpaI GTT'AAC
Cuts at: 2706 4053 2706
Size: 1347 5116
KpnI G_GTAC'C
Cuts at: 2443 3788 2443
Size: 1345 5118
MfeI C'AATT_G
Cuts at: 4062 4062
Size: 6463
MluI A'CGCG_T
Cuts at: 6339 6339
Size: 6463
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2550 5577 5761 1305
Size: 1245 3027 184 2007
Fragments arranged by size:
3027 2007 1245 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4641
Fragments arranged by size:
4641 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2906 4421 4780 4939 518
Size: 648 1740 1515 359 159 2042
Fragments arranged by size:
2042 1740 1515 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2956 2971 3127 3253 3589 3595 3879 4556
Size: 1350 15 156 126 336 6 284 677
Cuts at: 4556 5704 5949 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1350 1148 677 336 284 245 156
126 15 6
NcoI C'CATG_G
Cuts at: 513 1065 2037 513
Size: 552 972 4939
Fragments arranged by size:
4939 972 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6463
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6463
NotI GC'GGCC_GC
Cuts at: 3910 3910
Size: 6463
NspI r_CATG'y
Cuts at: 1132 1229 2534 3821 5366 1132
Size: 97 1305 1287 1545 2229
Fragments arranged by size:
2229 1545 1305 1287 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6463
PciI A'CATG_T
Cuts at: 5362 5362
Size: 6463
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2152 2717 1108
Size: 1044 565 4854
Fragments arranged by size:
4854 1044 565
PfoI T'CCnGG_A
Cuts at: 2121 2286 3294 3459 3528 2121
Size: 165 1008 165 69 5056
Fragments arranged by size:
5056 1008 165 165 69
PmeI GTTT'AAAC
Cuts at: 3767 3767
Size: 6463
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4532 4564 6045 6077 4532
Size: 32 1481 32 4918
Fragments arranged by size:
4918 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2382 2593 3645 2382
Size: 211 1052 5200
Fragments arranged by size:
5200 1052 211
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6463
PsiI TTA'TAA
Cuts at: 4033 4033
Size: 6463
PspOMI G'GGCC_C
Cuts at: 3117 3117
Size: 6463
PstI C_TGCA'G
Cuts at: 830 2850 3060 3126 3815 830
Size: 2020 210 66 689 3478
Fragments arranged by size:
3478 2020 689 210 66
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6463
PvuII CAG'CTG
Cuts at: 2956 2971 3127 3253 3595 2956
Size: 15 156 126 342 5824
Fragments arranged by size:
5824 342 156 126 15
SacI G_AGCT'C
Cuts at: 2283 3099 2283
Size: 816 5647
SalI G'TCGA_C
Cuts at: 1936 3805 1936
Size: 1869 4594
SbfI CC_TGCA'GG
Cuts at: 3815 3815
Size: 6463
ScaI AGT'ACT
Cuts at: 4711 6423 4711
Size: 1712 4751
SexAI A'CCwGG_T
Cuts at: 3369 3396 3369
Size: 27 6436
SfcI C'TryA_G
Cuts at: 826 1045 2846 3056 3122 3499 3811 4946 5627
Size: 219 1801 210 66 377 312 1135 681
Cuts at: 5627 5818 826
Size: 191 1471
Fragments arranged by size:
1801 1471 1135 681 377 312 219 210
191 66
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6463
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6463
SmaI CCC'GGG
Cuts at: 1872 2933 3790 1872
Size: 1061 857 4545
Fragments arranged by size:
4545 1061 857
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4567 5468 5730 820
Size: 197 926 2624 901 262 1553
Fragments arranged by size:
2624 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4254 493
Size: 3761 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6463
SphI G_CATG'C
Cuts at: 1132 2534 3821 1132
Size: 1402 1287 3774
Fragments arranged by size:
3774 1402 1287
SspI AAT'ATT
Cuts at: 5 52 2683 4387 5
Size: 47 2631 1704 2081
Fragments arranged by size:
2631 2081 1704 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6463
StyI C'CwwG_G
Cuts at: 513 1065 2037 2671 2726 3381 3902 513
Size: 552 972 634 55 655 521 3074
Fragments arranged by size:
3074 972 655 634 552 521 55
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2312 4490 4507 4660 4845 5290 325
Size: 1255 732 2178 17 153 185 445 1498
Fragments arranged by size:
2178 1498 1255 732 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3696 4709 6421
Size: 274 80 33 51 1443 1719 1013 1712
Cuts at: 6421 96
Size: 138
Fragments arranged by size:
1719 1712 1443 1013 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6423 6455 6423
Size: 32 6431
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6463
XbaI T'CTAG_A
Cuts at: 3799 3799
Size: 6463
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6463
XmaI C'CCGG_G
Cuts at: 1870 2931 3788 1870
Size: 1061 857 4545
Fragments arranged by size:
4545 1061 857
XmnI GAAnn'nnTTC
Cuts at: 2306 4592 2306
Size: 2286 4177
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4271 276
Size: 53 83 186 936 2737 2468
Fragments arranged by size:
2737 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspXI PsrI RsrII SacII SanDI SapI SfiI
SfoI SrfI SwaI XcmI