Restriction map for
pFN21AE1415
(Circular) (Six-base) MAPSORT of: pFN21AE1415.seq Check: 5884 from: 1 to: 5476
pFN21AE1415 5476 bp
With 182 enzymes: SgfI *
August 16, 2010 14:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2293 2324 2350 2465 2293
Size: 31 26 115 5304
Fragments arranged by size:
5304 115 31 26
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3286 278
Size: 53 83 186 936 1750 2468
Fragments arranged by size:
2468 1750 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2176 2819 1937
Size: 239 643 4594
Fragments arranged by size:
4594 643 239
Acc65I G'GTAC_C
Cuts at: 2797 2797
Size: 5476
AclI AA'CG_TT
Cuts at: 1563 3603 3976 1563
Size: 2040 373 3063
Fragments arranged by size:
3063 2040 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2478 3539 4923 5191 1632
Size: 846 1061 1384 268 1917
Fragments arranged by size:
1917 1384 1061 846 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5279
AflIII A'CryG_T
Cuts at: 1203 4375 5352 1203
Size: 3172 977 1327
Fragments arranged by size:
3172 1327 977
AgeI A'CCGG_T
Cuts at: 4307 4307
Size: 5476
AhdI GACnn_n'nnGTC
Cuts at: 4205 4205
Size: 5476
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5476
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2305 1184
Size: 1121 4355
AlwNI CAG_nnn'CTG
Cuts at: 2032 3219 4791 2032
Size: 1187 1572 2717
Fragments arranged by size:
2717 1572 1187
ApaI G_GGCC'C
Cuts at: 2349 2718 2349
Size: 369 5107
ApaLI G'TGCA_C
Cuts at: 3533 4689 3533
Size: 1156 4320
ApoI r'AATT_y
Cuts at: 1493 2785 3011 3163 5272 1493
Size: 1292 226 152 2109 1697
Fragments arranged by size:
2109 1697 1292 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4030 160
Size: 872 2998 1606
Fragments arranged by size:
2998 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5476
AvaI C'yCGr_G
Cuts at: 1870 1943 2150 2801 1870
Size: 73 207 651 4545
Fragments arranged by size:
4545 651 207 73
BamHI G'GATC_C
Cuts at: 2352 2806 3173 2352
Size: 454 367 4655
Fragments arranged by size:
4655 454 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2281 2797 4252 618
Size: 325 217 627 494 516 1455 1842
Fragments arranged by size:
1842 1455 627 516 494 325 217
BanII G_rGCy'C
Cuts at: 1391 2349 2718 2795 1391
Size: 958 369 77 4072
Fragments arranged by size:
4072 958 369 77
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4505
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3667 3701 1903
Size: 34 1730 34 3678
Fragments arranged by size:
3678 1730 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3368 4578 3368
Size: 1210 4266
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5476
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4087 136
Size: 107 122 71 631 605 2415 1525
Fragments arranged by size:
2415 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5471 5471
Size: 5476
BlpI GC'TnA_GC
Cuts at: 2010 2741 2771 2893 2010
Size: 731 30 122 4593
Fragments arranged by size:
4593 731 122 30
Bme1580I G_kGCm'C
Cuts at: 1790 2349 2403 2718 3537 4693 1790
Size: 559 54 315 819 1156 2573
Fragments arranged by size:
2573 1156 819 559 315 54
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5476
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3207 4165 442
Size: 416 53 83 399 24 1790 958 1753
Fragments arranged by size:
1790 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5476
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2203 4136 1239
Size: 964 1933 2579
Fragments arranged by size:
2579 1933 964
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3601 4466 4764 3601
Size: 865 298 4313
Fragments arranged by size:
4313 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4139 882
Size: 617 2640 2219
Fragments arranged by size:
2640 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3267 493
Size: 711 2063 2702
Fragments arranged by size:
2702 2063 711
BsaBI GATnn'nnATC
Cuts at: 5470 5470
Size: 5476
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3283 3665 4300 275
Size: 53 83 186 936 1750 382 635 1451
Fragments arranged by size:
1750 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3909 4307 4581 4728 5139 3909
Size: 398 274 147 411 4246
Fragments arranged by size:
4246 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2315 2345 1178
Size: 30 165 30 912 30 4309
Fragments arranged by size:
4309 912 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2687 1186
Size: 1501 3975
BseYI C'CCAG_C
Cuts at: 2650 2711 4679 5175 2650
Size: 61 1968 496 2951
Fragments arranged by size:
2951 1968 496 61
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4903
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2926 3687 4715 1540
Size: 275 186 925 761 1028 2301
Fragments arranged by size:
2301 1028 925 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2333 3537 3622 4693 2333
Size: 1204 85 1071 3116
Fragments arranged by size:
3116 1204 1071 85
BsmI GAATG_Cn'
Cuts at: 2197 2987 3080 2197
Size: 790 93 4593
Fragments arranged by size:
4593 790 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2333 2349 2403 2718 2795 3537 3622
Size: 399 543 16 54 315 77 742 85
Cuts at: 3622 4693 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 543 399 315 85 77
54 16
BspEI T'CCGG_A
Cuts at: 5139 5139
Size: 5476
BspHI T'CATG_A
Cuts at: 3365 5095 3365
Size: 1730 3746
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2293 2324 2350 2465 2831 844
Size: 1449 31 26 115 366 3489
Fragments arranged by size:
3489 1449 366 115 31 26
BsrBI CCG'CTC
Cuts at: 1394 1823 2386 3363 1394
Size: 429 563 977 3507
Fragments arranged by size:
3507 977 563 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2432 2578 3971 4145 65
Size: 1183 1184 146 1393 174 1396
Fragments arranged by size:
1396 1393 1184 1183 174 146
BsrFI r'CCGG_y
Cuts at: 1700 1810 4120 4307 1700
Size: 110 2310 187 2869
Fragments arranged by size:
2869 2310 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5476
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5476
BssSI C'ACGA_G
Cuts at: 3536 4548 3536
Size: 1012 4464
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5476
BstBI TT'CG_AA
Cuts at: 4280 4280
Size: 5476
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5476
BstYI r'GATC_y
Cuts at: 1972 2352 2806 3173 3558 3575 5027 5113 5125
Size: 380 454 367 385 17 1452 86 12
Cuts at: 5125 5471 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 454 385 380 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3985
Fragments arranged by size:
3985 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4737
Fragments arranged by size:
4737 683 56
BtsI GCAGTG_nn'
Cuts at: 2177 2520 2992 3786 3806 2177
Size: 343 472 794 20 3847
Fragments arranged by size:
3847 794 472 343 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5476
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5441
DraI TTT'AAA
Cuts at: 2780 2780
Size: 5476
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4483 809
Size: 507 3167 1802
Fragments arranged by size:
3167 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2623 2923 3812
Size: 54 1425 50 275 18 793 300 889
Cuts at: 3812 5441 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 793 300 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2923 1537
Size: 275 1111 4090
Fragments arranged by size:
4090 1111 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3406 1353
Size: 2053 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4063 4437 4583 387
Size: 1064 138 2474 374 146 1280
Fragments arranged by size:
2474 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2203 2478 3539 4136 4923 5191 1239
Size: 393 571 275 1061 597 787 268 1524
Fragments arranged by size:
1524 1061 787 597 571 393 275 268
EcoO109I rG'GnC_Cy
Cuts at: 2147 2345 2346 2715 2147
Size: 198 1 369 4908
Fragments arranged by size:
4908 369 198 1
EcoRI G'AATT_C
Cuts at: 2785 3163 2785
Size: 378 5098
FspI TGC'GCA
Cuts at: 2512 3982 2512
Size: 1470 4006
HaeII r_GCGC'y
Cuts at: 1678 4623 1678
Size: 2945 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4117 4149 4191 4223 1373
Size: 32 135 32 2545 32 42 32 2626
Fragments arranged by size:
2626 2545 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2177 2820 3066 669
Size: 988 281 239 643 246 3079
Fragments arranged by size:
3079 988 643 281 246 239
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5476
HpaI GTT'AAC
Cuts at: 3066 3066
Size: 5476
KpnI G_GTAC'C
Cuts at: 2801 2801
Size: 5476
MfeI C'AATT_G
Cuts at: 3075 3075
Size: 5476
MluI A'CGCG_T
Cuts at: 5352 5352
Size: 5476
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4590 4774 1305
Size: 3285 184 2007
Fragments arranged by size:
3285 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3654
Fragments arranged by size:
3654 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2232 2636 3434 3793 3952 518
Size: 648 1066 404 798 359 159 2042
Fragments arranged by size:
2042 1066 798 648 404 359 159
MspA1I CmG'CkG
Cuts at: 1606 2014 2029 2628 2892 3569 4717 4962 1606
Size: 408 15 599 264 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 599 408 264 245 15
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3985
Fragments arranged by size:
3985 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5476
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5476
NotI GC'GGCC_GC
Cuts at: 2923 2923
Size: 5476
NspI r_CATG'y
Cuts at: 1132 1229 2834 4379 1132
Size: 97 1605 1545 2229
Fragments arranged by size:
2229 1605 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2656 2718 1348
Size: 1308 62 4106
Fragments arranged by size:
4106 1308 62
PciI A'CATG_T
Cuts at: 4375 4375
Size: 5476
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2362 1108
Size: 1254 4222
PfoI T'CCnGG_A
Cuts at: 2307 2307
Size: 5476
PmeI GTTT'AAAC
Cuts at: 2780 2780
Size: 5476
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3545 3577 5058 5090 3545
Size: 32 1481 32 3931
Fragments arranged by size:
3931 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5476
PsiI TTA'TAA
Cuts at: 3046 3046
Size: 5476
PspOMI G'GGCC_C
Cuts at: 2345 2714 2345
Size: 369 5107
PstI C_TGCA'G
Cuts at: 830 2698 2828 830
Size: 1868 130 3478
Fragments arranged by size:
3478 1868 130
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5476
PvuII CAG'CTG
Cuts at: 2029 2029
Size: 5476
SalI G'TCGA_C
Cuts at: 1936 2175 2818 1936
Size: 239 643 4594
Fragments arranged by size:
4594 643 239
SbfI CC_TGCA'GG
Cuts at: 2828 2828
Size: 5476
ScaI AGT'ACT
Cuts at: 3724 5436 3724
Size: 1712 3764
SfcI C'TryA_G
Cuts at: 826 1045 2055 2608 2694 2824 3959 4640 4831
Size: 219 1010 553 86 130 1135 681 191
Cuts at: 4831 826
Size: 1471
Fragments arranged by size:
1471 1135 1010 681 553 219 191 130
86
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5476
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5476
SmaI CCC'GGG
Cuts at: 1872 2803 1872
Size: 931 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3580 4481 4743 820
Size: 197 926 1637 901 262 1553
Fragments arranged by size:
1637 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3267 493
Size: 2774 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5476
SphI G_CATG'C
Cuts at: 1132 2834 1132
Size: 1702 3774
SspI AAT'ATT
Cuts at: 5 52 3400 5
Size: 47 3348 2081
Fragments arranged by size:
3348 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5476
StyI C'CwwG_G
Cuts at: 513 1065 2004 2915 513
Size: 552 939 911 3074
Fragments arranged by size:
3074 939 911 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2038 2572 3503 3520 3673 3858 4303
Size: 1255 458 534 931 17 153 185 445
Cuts at: 4303 325
Size: 1498
Fragments arranged by size:
1498 1255 931 534 458 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3722 5434 96
Size: 274 80 33 51 1443 1745 1712 138
Fragments arranged by size:
1745 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2254 2286 5436 5468 2254
Size: 32 3150 32 2262
Fragments arranged by size:
3150 2262 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5476
XbaI T'CTAG_A
Cuts at: 2812 2812
Size: 5476
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2674 2674
Size: 5476
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5476
XmaI C'CCGG_G
Cuts at: 1870 2801 1870
Size: 931 4545
XmnI GAAnn'nnTTC
Cuts at: 3605 3605
Size: 5476
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3284 276
Size: 53 83 186 936 1750 2468
Fragments arranged by size:
2468 1750 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI