Restriction map for
pFN21AE1414
(Circular) (Six-base) MAPSORT of: pFN21AE1414.seq Check: 762 from: 1 to: 5377
pFN21AE1414 5377 bp
With 182 enzymes: SgfI *
July 6, 2012 17:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2498 2624 2646 2498
Size: 126 22 5229
Fragments arranged by size:
5229 126 22
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3187 278
Size: 53 83 186 936 1651 2468
Fragments arranged by size:
2468 1651 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2720 1937
Size: 783 4594
Acc65I G'GTAC_C
Cuts at: 2625 2698 2625
Size: 73 5304
AclI AA'CG_TT
Cuts at: 1563 3504 3877 1563
Size: 1941 373 3063
Fragments arranged by size:
3063 1941 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3440 4824 5092 1632
Size: 1808 1384 268 1917
Fragments arranged by size:
1917 1808 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5180
AflIII A'CryG_T
Cuts at: 1203 4276 5253 1203
Size: 3073 977 1327
Fragments arranged by size:
3073 1327 977
AgeI A'CCGG_T
Cuts at: 4208 4208
Size: 5377
AhdI GACnn_n'nnGTC
Cuts at: 4106 4106
Size: 5377
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5377
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2113 1184
Size: 929 4448
AlwNI CAG_nnn'CTG
Cuts at: 2195 3120 4692 2195
Size: 925 1572 2880
Fragments arranged by size:
2880 1572 925
ApaLI G'TGCA_C
Cuts at: 3434 4590 3434
Size: 1156 4221
ApoI r'AATT_y
Cuts at: 1493 2686 2912 3064 5173 1493
Size: 1193 226 152 2109 1697
Fragments arranged by size:
2109 1697 1193 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3931 160
Size: 872 2899 1606
Fragments arranged by size:
2899 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5377
AvaI C'yCGr_G
Cuts at: 1870 1943 2074 2702 1870
Size: 73 131 628 4545
Fragments arranged by size:
4545 628 131 73
BamHI G'GATC_C
Cuts at: 2707 3074 2707
Size: 367 5010
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2612 2625 2698 4153 618
Size: 325 217 627 825 13 73 1455 1842
Fragments arranged by size:
1842 1455 825 627 325 217 73 13
BanII G_rGCy'C
Cuts at: 1391 2018 2412 2696 1391
Size: 627 394 284 4072
Fragments arranged by size:
4072 627 394 284
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2374 928
Size: 971 475 3931
Fragments arranged by size:
3931 971 475
BbvCI CC'TCA_GC
Cuts at: 2115 2115
Size: 5377
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2386 2420 3568 3602 1903
Size: 34 50 34 365 34 1148 34 3678
Fragments arranged by size:
3678 1148 365 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3269 4479 3269
Size: 1210 4167
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5377
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2077 3988 136
Size: 107 122 71 631 605 405 1911 1525
Fragments arranged by size:
1911 1525 631 605 405 122 107 71
BglII A'GATC_T
Cuts at: 5372 5372
Size: 5377
BlpI GC'TnA_GC
Cuts at: 2580 2794 2580
Size: 214 5163
Bme1580I G_kGCm'C
Cuts at: 1790 3438 4594 1790
Size: 1648 1156 2573
Fragments arranged by size:
2573 1648 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5377
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3108 4066 442
Size: 416 53 83 399 24 1691 958 1753
Fragments arranged by size:
1753 1691 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5377
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2146 4037 1239
Size: 907 1891 2579
Fragments arranged by size:
2579 1891 907
Bpu10I CC'TnA_GC
Cuts at: 2115 2115
Size: 5377
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3502 4367 4665 3502
Size: 865 298 4214
Fragments arranged by size:
4214 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4040 882
Size: 617 2541 2219
Fragments arranged by size:
2541 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3168 493
Size: 711 1964 2702
Fragments arranged by size:
2702 1964 711
BsaBI GATnn'nnATC
Cuts at: 5371 5371
Size: 5377
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3184 3566 4201 275
Size: 53 83 186 936 1651 382 635 1451
Fragments arranged by size:
1651 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2217 3810 4208 4482 4629 5040 2217
Size: 1593 398 274 147 411 2554
Fragments arranged by size:
2554 1593 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2408 2438 1178
Size: 30 165 30 1005 30 4117
Fragments arranged by size:
4117 1005 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5377
BseYI C'CCAG_C
Cuts at: 2034 2080 4580 5076 2034
Size: 46 2500 496 2335
Fragments arranged by size:
2500 2335 496 46
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2208 2453 1163
Size: 573 472 245 4087
Fragments arranged by size:
4087 573 472 245
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2827 3588 4616 1540
Size: 275 186 826 761 1028 2301
Fragments arranged by size:
2301 1028 826 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3438 3523 4594 3438
Size: 85 1071 4221
Fragments arranged by size:
4221 1071 85
BsmI GAATG_Cn'
Cuts at: 2888 2981 2888
Size: 93 5284
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2018 2412 2696 3438 3523 4594 1391
Size: 399 228 394 284 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 394 284 228 85
BspEI T'CCGG_A
Cuts at: 5040 5040
Size: 5377
BspHI T'CATG_A
Cuts at: 3266 4996 3266
Size: 1730 3647
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2498 2624 2646 2732 844
Size: 1654 126 22 86 3489
Fragments arranged by size:
3489 1654 126 86 22
BsrBI CCG'CTC
Cuts at: 1394 1823 3264 1394
Size: 429 1441 3507
Fragments arranged by size:
3507 1441 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3872 4046 65
Size: 1183 2624 174 1396
Fragments arranged by size:
2624 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4021 4208 1700
Size: 110 2211 187 2869
Fragments arranged by size:
2869 2211 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5377
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5377
BssSI C'ACGA_G
Cuts at: 3437 4449 3437
Size: 1012 4365
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2195 794
Size: 1401 3976
BstBI TT'CG_AA
Cuts at: 4181 4181
Size: 5377
BstEII G'GTnAC_C
Cuts at: 1184 2570 1184
Size: 1386 3991
BstYI r'GATC_y
Cuts at: 1972 2707 3074 3459 3476 4928 5014 5026 5372
Size: 735 367 385 17 1452 86 12 346
Cuts at: 5372 1972
Size: 1977
Fragments arranged by size:
1977 1452 735 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4825
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4638
Fragments arranged by size:
4638 683 56
BtsI GCAGTG_nn'
Cuts at: 2893 3687 3707 2893
Size: 794 20 4563
Fragments arranged by size:
4563 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5377
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5342
DraI TTT'AAA
Cuts at: 2681 2681
Size: 5377
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4384 809
Size: 507 3068 1802
Fragments arranged by size:
3068 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2181 2824 3713
Size: 54 1425 50 275 18 351 643 889
Cuts at: 3713 5342 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 643 351 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2824 1537
Size: 275 1012 4090
Fragments arranged by size:
4090 1012 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2305 3307 1353
Size: 952 1002 3423
Fragments arranged by size:
3423 1002 952
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3964 4338 4484 387
Size: 1064 138 2375 374 146 1280
Fragments arranged by size:
2375 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2146 3440 4037 4824 5092 1239
Size: 393 514 1294 597 787 268 1524
Fragments arranged by size:
1524 1294 787 597 514 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2362 2419 2362
Size: 57 5320
EcoRI G'AATT_C
Cuts at: 2686 3064 2686
Size: 378 4999
FspI TGC'GCA
Cuts at: 3883 3883
Size: 5377
HaeII r_GCGC'y
Cuts at: 1678 4524 1678
Size: 2846 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2408 2428 2440 2460 4018
Size: 32 135 32 836 20 12 20 1558
Cuts at: 4018 4050 4092 4124 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1558 836 135 42 32 32 32
32 20 20 12
HincII GTy'rAC
Cuts at: 669 1657 1938 2608 2662 2721 2967 669
Size: 988 281 670 54 59 246 3079
Fragments arranged by size:
3079 988 670 281 246 59 54
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5377
HpaI GTT'AAC
Cuts at: 2967 2967
Size: 5377
KpnI G_GTAC'C
Cuts at: 2629 2702 2629
Size: 73 5304
MfeI C'AATT_G
Cuts at: 2976 2976
Size: 5377
MluI A'CGCG_T
Cuts at: 5253 5253
Size: 5377
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2532 4491 4675 1305
Size: 1227 1959 184 2007
Fragments arranged by size:
2007 1959 1227 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3555
Fragments arranged by size:
3555 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3335 3694 3853 518
Size: 648 2169 359 159 2042
Fragments arranged by size:
2169 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2097 2622 2793 3470 4618 4863 1606
Size: 491 525 171 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 525 491 245 171
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4825
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5377
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5377
NotI GC'GGCC_GC
Cuts at: 2824 2824
Size: 5377
NspI r_CATG'y
Cuts at: 1132 1229 2094 2606 2735 4280 1132
Size: 97 865 512 129 1545 2229
Fragments arranged by size:
2229 1545 865 512 129 97
PasI CC'CwG_GG
Cuts at: 1348 2412 1348
Size: 1064 4313
PciI A'CATG_T
Cuts at: 4276 4276
Size: 5377
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5377
PmeI GTTT'AAAC
Cuts at: 2681 2681
Size: 5377
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2547 2579 3446 3478 4959 4991 2547
Size: 32 867 32 1481 32 2933
Fragments arranged by size:
2933 1481 867 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2362 2419 2362
Size: 57 5320
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5377
PsiI TTA'TAA
Cuts at: 2947 2947
Size: 5377
PstI C_TGCA'G
Cuts at: 830 2621 2729 830
Size: 1791 108 3478
Fragments arranged by size:
3478 1791 108
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5377
PvuII CAG'CTG
Cuts at: 2097 2622 2097
Size: 525 4852
SalI G'TCGA_C
Cuts at: 1936 2719 1936
Size: 783 4594
SbfI CC_TGCA'GG
Cuts at: 2729 2729
Size: 5377
ScaI AGT'ACT
Cuts at: 3625 5337 3625
Size: 1712 3665
SfcI C'TryA_G
Cuts at: 826 1045 2617 2725 3860 4541 4732 826
Size: 219 1572 108 1135 681 191 1471
Fragments arranged by size:
1572 1471 1135 681 219 191 108
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5377
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5377
SmaI CCC'GGG
Cuts at: 1872 2076 2704 1872
Size: 204 628 4545
Fragments arranged by size:
4545 628 204
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3481 4382 4644 820
Size: 197 926 1538 901 262 1553
Fragments arranged by size:
1553 1538 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3168 493
Size: 2675 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5377
SphI G_CATG'C
Cuts at: 1132 2094 2735 1132
Size: 962 641 3774
Fragments arranged by size:
3774 962 641
SspI AAT'ATT
Cuts at: 5 52 3301 5
Size: 47 3249 2081
Fragments arranged by size:
3249 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5377
StyI C'CwwG_G
Cuts at: 513 1065 2427 2816 513
Size: 552 1362 389 3074
Fragments arranged by size:
3074 1362 552 389
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2026 2072 3404 3421 3574 3759 4204
Size: 1255 446 46 1332 17 153 185 445
Cuts at: 4204 325
Size: 1498
Fragments arranged by size:
1498 1332 1255 446 445 185 153 46
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3623 5335 96
Size: 274 80 33 51 1443 1646 1712 138
Fragments arranged by size:
1712 1646 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5337 5369 5337
Size: 32 5345
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5377
XbaI T'CTAG_A
Cuts at: 2713 2713
Size: 5377
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2468 2468
Size: 5377
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5377
XmaI C'CCGG_G
Cuts at: 1870 2074 2702 1870
Size: 204 628 4545
Fragments arranged by size:
4545 628 204
XmnI GAAnn'nnTTC
Cuts at: 3506 3506
Size: 5377
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3185 276
Size: 53 83 186 936 1651 2468
Fragments arranged by size:
2468 1651 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI