Restriction map for
pFN21AE1413
(Circular) (Six-base) MAPSORT of: pFN21AE1413.seq Check: 2636 from: 1 to: 6205
pFN21AE1413 6205 bp
With 182 enzymes: SgfI *
August 16, 2010 14:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4015 278
Size: 53 83 186 936 2479 2468
Fragments arranged by size:
2479 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3548 1937
Size: 1611 4594
Acc65I G'GTAC_C
Cuts at: 3079 3526 3079
Size: 447 5758
AclI AA'CG_TT
Cuts at: 1563 4332 4705 1563
Size: 2769 373 3063
Fragments arranged by size:
3063 2769 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2477 2654 3296 4268 5652 5920 1632
Size: 845 177 642 972 1384 268 1917
Fragments arranged by size:
1917 1384 972 845 642 268 177
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6008
AflIII A'CryG_T
Cuts at: 1203 5104 6081 1203
Size: 3901 977 1327
Fragments arranged by size:
3901 1327 977
AgeI A'CCGG_T
Cuts at: 5036 5036
Size: 6205
AhdI GACnn_n'nnGTC
Cuts at: 4934 4934
Size: 6205
AleI CACnn'nnGTG
Cuts at: 1166 2711 2798 1166
Size: 1545 87 4573
Fragments arranged by size:
4573 1545 87
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6205
AlwNI CAG_nnn'CTG
Cuts at: 2381 2548 2632 3091 3359 3948 5520 2381
Size: 167 84 459 268 589 1572 3066
Fragments arranged by size:
3066 1572 589 459 268 167 84
ApaLI G'TGCA_C
Cuts at: 4262 5418 4262
Size: 1156 5049
ApoI r'AATT_y
Cuts at: 1493 3254 3514 3740 3892 6001 1493
Size: 1761 260 226 152 2109 1697
Fragments arranged by size:
2109 1761 1697 260 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4759 160
Size: 872 3727 1606
Fragments arranged by size:
3727 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6205
AvaI C'yCGr_G
Cuts at: 1870 1943 2552 3530 1870
Size: 73 609 978 4545
Fragments arranged by size:
4545 978 609 73
BamHI G'GATC_C
Cuts at: 2101 3535 3902 2101
Size: 1434 367 4404
Fragments arranged by size:
4404 1434 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3079 3526 4981 618
Size: 325 217 627 1292 447 1455 1842
Fragments arranged by size:
1842 1455 1292 627 447 325 217
BanII G_rGCy'C
Cuts at: 1391 2286 2787 3524 1391
Size: 895 501 737 4072
Fragments arranged by size:
4072 895 737 501
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2909 3114 3466 928
Size: 971 1010 205 352 3667
Fragments arranged by size:
3667 1010 971 352 205
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4396 4430 1903
Size: 34 2459 34 3678
Fragments arranged by size:
3678 2459 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4097 5307 4097
Size: 1210 4995
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6205
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4816 136
Size: 107 122 71 631 605 3144 1525
Fragments arranged by size:
3144 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2673 3307 6200 2673
Size: 634 2893 2678
Fragments arranged by size:
2893 2678 634
BlpI GC'TnA_GC
Cuts at: 3622 3622
Size: 6205
Bme1580I G_kGCm'C
Cuts at: 1790 2973 4266 5422 1790
Size: 1183 1293 1156 2573
Fragments arranged by size:
2573 1293 1183 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6205
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2370 3369 3936
Size: 416 53 83 399 24 953 999 567
Cuts at: 3936 4894 442
Size: 958 1753
Fragments arranged by size:
1753 999 958 953 567 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6205
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1996 2074 2204 2603 3182 4865 1239
Size: 757 78 130 399 579 1683 2579
Fragments arranged by size:
2579 1683 757 579 399 130 78
Bpu10I CC'TnA_GC
Cuts at: 2756 2756
Size: 6205
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4330 5195 5493 4330
Size: 865 298 5042
Fragments arranged by size:
5042 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2489 4868 882
Size: 617 990 2379 2219
Fragments arranged by size:
2379 2219 990 617
BsaAI yAC'GTr
Cuts at: 493 1204 3996 493
Size: 711 2792 2702
Fragments arranged by size:
2792 2702 711
BsaBI GATnn'nnATC
Cuts at: 6199 6199
Size: 6205
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2772 4012 4394 5029
Size: 53 83 186 936 1239 1240 382 635
Cuts at: 5029 275
Size: 1451
Fragments arranged by size:
1451 1240 1239 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4638 5036 5310 5457 5868 4638
Size: 398 274 147 411 4975
Fragments arranged by size:
4975 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2326 2356 1178
Size: 30 165 30 923 30 5027
Fragments arranged by size:
5027 923 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2056 2861 1186
Size: 870 805 4530
Fragments arranged by size:
4530 870 805
BseYI C'CCAG_C
Cuts at: 2051 2325 2349 2558 5408 5904 2051
Size: 274 24 209 2850 496 2352
Fragments arranged by size:
2850 2352 496 274 209 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3443 1163
Size: 573 1707 3925
Fragments arranged by size:
3925 1707 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3655 4416 5444 1540
Size: 275 186 1654 761 1028 2301
Fragments arranged by size:
2301 1654 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2787 4266 4351 5422 2787
Size: 1479 85 1071 3570
Fragments arranged by size:
3570 1479 1071 85
BsmI GAATG_Cn'
Cuts at: 2722 3716 3809 2722
Size: 994 93 5118
Fragments arranged by size:
5118 994 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2286 2787 2973 3524 4266 4351 5422
Size: 399 496 501 186 551 742 85 1071
Cuts at: 5422 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 551 501 496 399 186
85
BspEI T'CCGG_A
Cuts at: 5868 5868
Size: 6205
BspHI T'CATG_A
Cuts at: 4094 5824 4094
Size: 1730 4475
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3560 844
Size: 2716 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4092 1394
Size: 429 2269 3507
Fragments arranged by size:
3507 2269 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2487 4700 4874 65
Size: 1183 1239 2213 174 1396
Fragments arranged by size:
2213 1396 1239 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2829 4849 5036 1700
Size: 110 1019 2020 187 2869
Fragments arranged by size:
2869 2020 1019 187 110
BsrGI T'GTAC_A
Cuts at: 96 2733 96
Size: 2637 3568
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6205
BssSI C'ACGA_G
Cuts at: 4265 5277 4265
Size: 1012 5193
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6205
BstBI TT'CG_AA
Cuts at: 5009 5009
Size: 6205
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6205
BstXI CCAn_nnnn'nTGG
Cuts at: 3394 3394
Size: 6205
BstYI r'GATC_y
Cuts at: 1972 2101 2673 3307 3535 3902 4287 4304 5756
Size: 129 572 634 228 367 385 17 1452
Cuts at: 5756 5842 5854 6200 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 634 572 385 367 346 228
129 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4714
Fragments arranged by size:
4714 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2509 3338 487
Size: 683 56 1283 829 3354
Fragments arranged by size:
3354 1283 829 683 56
BtsI GCAGTG_nn'
Cuts at: 3721 4515 4535 3721
Size: 794 20 5391
Fragments arranged by size:
5391 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6205
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6170
DraI TTT'AAA
Cuts at: 3509 3509
Size: 6205
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5212 809
Size: 507 3896 1802
Fragments arranged by size:
3896 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2694 2802 3652
Size: 54 1425 50 275 18 864 108 850
Cuts at: 3652 4541 6170 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 864 850 275 108 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3652 1537
Size: 275 1840 4090
Fragments arranged by size:
4090 1840 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3443 4135 1353
Size: 2090 692 3423
Fragments arranged by size:
3423 2090 692
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3373 4792 5166 5312 387
Size: 1064 138 1784 1419 374 146 1280
Fragments arranged by size:
1784 1419 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2785 2785
Size: 6205
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 1996 2074 2204 2477 2603 2654 3182
Size: 393 364 78 130 273 126 51 528
Cuts at: 3182 3296 4268 4865 5652 5920 1239
Size: 114 972 597 787 268 1524
Fragments arranged by size:
1524 972 787 597 528 393 364 273
268 130 126 114 78 51
EcoNI CCTnn'n_nnAGG
Cuts at: 2540 2540
Size: 6205
EcoO109I rG'GnC_Cy
Cuts at: 2364 2378 2545 2629 3301 2364
Size: 14 167 84 672 5268
Fragments arranged by size:
5268 672 167 84 14
EcoRI G'AATT_C
Cuts at: 3254 3514 3892 3254
Size: 260 378 5567
Fragments arranged by size:
5567 378 260
FspI TGC'GCA
Cuts at: 4711 4711
Size: 6205
HaeII r_GCGC'y
Cuts at: 1678 5352 1678
Size: 3674 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2048 2080 3218 3250 4846
Size: 32 135 32 476 32 1138 32 1596
Cuts at: 4846 4878 4920 4952 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1596 1138 476 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3549 3795 669
Size: 988 281 1611 246 3079
Fragments arranged by size:
3079 1611 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6205
HpaI GTT'AAC
Cuts at: 3795 3795
Size: 6205
KpnI G_GTAC'C
Cuts at: 3083 3530 3083
Size: 447 5758
MfeI C'AATT_G
Cuts at: 3804 3804
Size: 6205
MluI A'CGCG_T
Cuts at: 6081 6081
Size: 6205
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2329 5319 5503 1305
Size: 1024 2990 184 2007
Fragments arranged by size:
2990 2007 1024 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2696 2804 10
Size: 54 1425 343 864 108 3411
Fragments arranged by size:
3411 1425 864 343 108 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2711 2798 3209 3392 4163 4522 4681
Size: 648 1545 87 411 183 771 359 159
Cuts at: 4681 518
Size: 2042
Fragments arranged by size:
2042 1545 771 648 411 359 183 159
87
MspA1I CmG'CkG
Cuts at: 1606 3043 3621 4298 5446 5691 1606
Size: 1437 578 677 1148 245 2120
Fragments arranged by size:
2120 1437 1148 677 578 245
NaeI GCC'GGC
Cuts at: 2831 2831
Size: 6205
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4714
Fragments arranged by size:
4714 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6205
NgoMIV G'CCGG_C
Cuts at: 2829 2829
Size: 6205
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6205
NotI GC'GGCC_GC
Cuts at: 3652 3652
Size: 6205
NspI r_CATG'y
Cuts at: 1132 1229 3563 5108 1132
Size: 97 2334 1545 2229
Fragments arranged by size:
2334 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3498 1348
Size: 2150 4055
PciI A'CATG_T
Cuts at: 5104 5104
Size: 6205
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6205
PfoI T'CCnGG_A
Cuts at: 2181 2181
Size: 6205
PmeI GTTT'AAAC
Cuts at: 3509 3509
Size: 6205
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3108 3140 4274 4306 5787 5819 3108
Size: 32 1134 32 1481 32 3494
Fragments arranged by size:
3494 1481 1134 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2364 2378 2545 3301 2364
Size: 14 167 756 5268
Fragments arranged by size:
5268 756 167 14
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6205
PsiI TTA'TAA
Cuts at: 2963 3775 2963
Size: 812 5393
PstI C_TGCA'G
Cuts at: 830 3048 3379 3557 830
Size: 2218 331 178 3478
Fragments arranged by size:
3478 2218 331 178
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6205
PvuII CAG'CTG
Cuts at: 3043 3043
Size: 6205
SacI G_AGCT'C
Cuts at: 2787 2787
Size: 6205
SalI G'TCGA_C
Cuts at: 1936 3547 1936
Size: 1611 4594
SbfI CC_TGCA'GG
Cuts at: 3557 3557
Size: 6205
ScaI AGT'ACT
Cuts at: 4453 6165 4453
Size: 1712 4493
SfcI C'TryA_G
Cuts at: 826 1045 3044 3375 3553 4688 5369 5560 826
Size: 219 1999 331 178 1135 681 191 1471
Fragments arranged by size:
1999 1471 1135 681 331 219 191 178
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6205
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6205
SmaI CCC'GGG
Cuts at: 1872 3532 1872
Size: 1660 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4309 5210 5472 820
Size: 197 926 2366 901 262 1553
Fragments arranged by size:
2366 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3996 493
Size: 3503 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6205
SphI G_CATG'C
Cuts at: 1132 3563 1132
Size: 2431 3774
SspI AAT'ATT
Cuts at: 5 52 4129 5
Size: 47 4077 2081
Fragments arranged by size:
4077 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6205
StyI C'CwwG_G
Cuts at: 513 1065 2004 2604 3030 3644 513
Size: 552 939 600 426 614 3074
Fragments arranged by size:
3074 939 614 600 552 426
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2306 2327 2788 4232 4249 4402 4587
Size: 1255 726 21 461 1444 17 153 185
Cuts at: 4587 5032 325
Size: 445 1498
Fragments arranged by size:
1498 1444 1255 726 461 445 185 153
21 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2733 4451 6163
Size: 274 80 33 51 1443 756 1718 1712
Cuts at: 6163 96
Size: 138
Fragments arranged by size:
1718 1712 1443 756 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6165 6197 6165
Size: 32 6173
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6205
XbaI T'CTAG_A
Cuts at: 3541 3541
Size: 6205
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2357 2578 2357
Size: 221 5984
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6205
XmaI C'CCGG_G
Cuts at: 1870 3530 1870
Size: 1660 4545
XmnI GAAnn'nnTTC
Cuts at: 2639 2940 4334 2639
Size: 301 1394 4510
Fragments arranged by size:
4510 1394 301
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4013 276
Size: 53 83 186 936 2479 2468
Fragments arranged by size:
2479 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoRV FalI FseI FspAI KasI NarI NruI NsiI
PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI