Restriction map for
pFN21AE1411
(Circular) (Six-base) MAPSORT of: pFN21AE1411.seq Check: 5648 from: 1 to: 5302
pFN21AE1411 5302 bp
With 182 enzymes: SgfI *
August 16, 2010 14:46 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2291 2470 2291
Size: 179 5123
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3112 278
Size: 53 83 186 936 1576 2468
Fragments arranged by size:
2468 1576 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2645 1937
Size: 708 4594
Acc65I G'GTAC_C
Cuts at: 2623 2623
Size: 5302
AclI AA'CG_TT
Cuts at: 1563 3429 3802 1563
Size: 1866 373 3063
Fragments arranged by size:
3063 1866 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2069 2282 3365 4749 5017 1632
Size: 437 213 1083 1384 268 1917
Fragments arranged by size:
1917 1384 1083 437 268 213
AfeI AGC'GCT
Cuts at: 2513 2513
Size: 5302
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5105
AflIII A'CryG_T
Cuts at: 1203 2592 4201 5178 1203
Size: 1389 1609 977 1327
Fragments arranged by size:
1609 1389 1327 977
AgeI A'CCGG_T
Cuts at: 4133 4133
Size: 5302
AhdI GACnn_n'nnGTC
Cuts at: 4031 4031
Size: 5302
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5302
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5302
AlwNI CAG_nnn'CTG
Cuts at: 2383 2542 3045 4617 2383
Size: 159 503 1572 3068
Fragments arranged by size:
3068 1572 503 159
ApaI G_GGCC'C
Cuts at: 2201 2390 2201
Size: 189 5113
ApaLI G'TGCA_C
Cuts at: 3359 4515 3359
Size: 1156 4146
ApoI r'AATT_y
Cuts at: 1493 2611 2837 2989 5098 1493
Size: 1118 226 152 2109 1697
Fragments arranged by size:
2109 1697 1118 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3856 160
Size: 872 2824 1606
Fragments arranged by size:
2824 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5302
AvaI C'yCGr_G
Cuts at: 1870 1943 2627 1870
Size: 73 684 4545
Fragments arranged by size:
4545 684 73
BamHI G'GATC_C
Cuts at: 2319 2632 2999 2319
Size: 313 367 4622
Fragments arranged by size:
4622 367 313
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2623 4078 618
Size: 325 217 627 836 1455 1842
Fragments arranged by size:
1842 1455 836 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2201 2390 2403 2621 1391
Size: 810 189 13 218 4072
Fragments arranged by size:
4072 810 218 189 13
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4331
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2279 2313 3493 3527 1903
Size: 34 342 34 1180 34 3678
Fragments arranged by size:
3678 1180 342 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3194 4404 3194
Size: 1210 4092
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5302
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 3913 136
Size: 107 122 71 631 605 337 1904 1525
Fragments arranged by size:
1904 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 5297 5297
Size: 5302
BlpI GC'TnA_GC
Cuts at: 2719 2719
Size: 5302
Bme1580I G_kGCm'C
Cuts at: 1790 2201 2390 3363 4519 1790
Size: 411 189 973 1156 2573
Fragments arranged by size:
2573 1156 973 411 189
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5302
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2325 3033 3991
Size: 416 53 83 399 24 908 708 958
Cuts at: 3991 442
Size: 1753
Fragments arranged by size:
1753 958 908 708 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5302
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2066 3962 1239
Size: 827 1896 2579
Fragments arranged by size:
2579 1896 827
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3427 4292 4590 3427
Size: 865 298 4139
Fragments arranged by size:
4139 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3965 882
Size: 617 2466 2219
Fragments arranged by size:
2466 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3093 493
Size: 711 1889 2702
Fragments arranged by size:
2702 1889 711
BsaBI GATnn'nnATC
Cuts at: 5296 5296
Size: 5302
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3109 3491 4126 275
Size: 53 83 186 936 1576 382 635 1451
Fragments arranged by size:
1576 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2157 3735 4133 4407 4554 4965 2157
Size: 1578 398 274 147 411 2494
Fragments arranged by size:
2494 1578 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2013 2043 1178
Size: 30 165 30 610 30 4437
Fragments arranged by size:
4437 610 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5302
BseYI C'CCAG_C
Cuts at: 2050 2383 4505 5001 2050
Size: 333 2122 496 2351
Fragments arranged by size:
2351 2122 496 333
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2076 2492 2528 2573 1163
Size: 573 340 416 36 45 3892
Fragments arranged by size:
3892 573 416 340 45 36
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2752 3513 4541 1540
Size: 275 186 751 761 1028 2301
Fragments arranged by size:
2301 1028 761 751 275 186
BsiHKAI G_wGCw'C
Cuts at: 3363 3448 4519 3363
Size: 85 1071 4146
Fragments arranged by size:
4146 1071 85
BsmI GAATG_Cn'
Cuts at: 2813 2906 2813
Size: 93 5209
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2201 2390 2403 2621 3363 3448 4519
Size: 399 411 189 13 218 742 85 1071
Cuts at: 4519 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 411 399 218 189 85
13
BspEI T'CCGG_A
Cuts at: 2157 4965 2157
Size: 2808 2494
BspHI T'CATG_A
Cuts at: 3191 4921 3191
Size: 1730 3572
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2291 2301 2470 2657 844
Size: 1447 10 169 187 3489
Fragments arranged by size:
3489 1447 187 169 10
BsrBI CCG'CTC
Cuts at: 1394 1823 2034 3189 1394
Size: 429 211 1155 3507
Fragments arranged by size:
3507 1155 429 211
BsrDI GCAATG_nn'
Cuts at: 65 1248 3797 3971 65
Size: 1183 2549 174 1396
Fragments arranged by size:
2549 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2355 3946 4133 1700
Size: 110 545 1591 187 2869
Fragments arranged by size:
2869 1591 545 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5302
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5302
BssSI C'ACGA_G
Cuts at: 3362 4374 3362
Size: 1012 4290
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5302
BstBI TT'CG_AA
Cuts at: 4106 4106
Size: 5302
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5302
BstYI r'GATC_y
Cuts at: 1972 2319 2632 2999 3384 3401 4853 4939 4951
Size: 347 313 367 385 17 1452 86 12
Cuts at: 4951 5297 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 385 367 347 346 313 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2403 513
Size: 552 939 399 3412
Fragments arranged by size:
3412 939 552 399
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4563
Fragments arranged by size:
4563 683 56
BtsI GCAGTG_nn'
Cuts at: 2818 3612 3632 2818
Size: 794 20 4488
Fragments arranged by size:
4488 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5302
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5267
DraI TTT'AAA
Cuts at: 2606 2606
Size: 5302
DraIII CAC_nnn'GTG
Cuts at: 2470 2470
Size: 5302
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4309 809
Size: 507 2993 1802
Fragments arranged by size:
2993 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2749 3638 5267
Size: 54 1425 50 275 18 919 889 1629
Cuts at: 5267 8
Size: 43
Fragments arranged by size:
1629 1425 919 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2749 1537
Size: 275 937 4090
Fragments arranged by size:
4090 937 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2271 3232 1353
Size: 918 961 3423
Fragments arranged by size:
3423 961 918
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3889 4263 4409 387
Size: 1064 138 2300 374 146 1280
Fragments arranged by size:
2300 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2066 2069 2282 3365 3962 4749 5017
Size: 393 434 3 213 1083 597 787 268
Cuts at: 5017 1239
Size: 1524
Fragments arranged by size:
1524 1083 787 597 434 393 268 213
3
EcoNI CCTnn'n_nnAGG
Cuts at: 2096 2248 2096
Size: 152 5150
EcoO109I rG'GnC_Cy
Cuts at: 2197 2198 2387 2197
Size: 1 189 5112
Fragments arranged by size:
5112 189 1
EcoRI G'AATT_C
Cuts at: 2611 2989 2611
Size: 378 4924
EcoRV GAT'ATC
Cuts at: 2182 2437 2182
Size: 255 5047
FspI TGC'GCA
Cuts at: 3808 3808
Size: 5302
HaeII r_GCGC'y
Cuts at: 1678 2515 4449 1678
Size: 837 1934 2531
Fragments arranged by size:
2531 1934 837
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3943 3975 4017 4049 1373
Size: 32 135 32 2371 32 42 32 2626
Fragments arranged by size:
2626 2371 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2646 2892 669
Size: 988 281 708 246 3079
Fragments arranged by size:
3079 988 708 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5302
HpaI GTT'AAC
Cuts at: 2892 2892
Size: 5302
KpnI G_GTAC'C
Cuts at: 2627 2627
Size: 5302
MfeI C'AATT_G
Cuts at: 2901 2901
Size: 5302
MluI A'CGCG_T
Cuts at: 5178 5178
Size: 5302
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2577 4416 4600 1305
Size: 1272 1839 184 2007
Fragments arranged by size:
2007 1839 1272 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3480
Fragments arranged by size:
3480 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3260 3619 3778 518
Size: 648 2094 359 159 2042
Fragments arranged by size:
2094 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2371 2445 2718 3395 4543 4788 1606
Size: 765 74 273 677 1148 245 2120
Fragments arranged by size:
2120 1148 765 677 273 245 74
NcoI C'CATG_G
Cuts at: 513 1065 2004 2403 513
Size: 552 939 399 3412
Fragments arranged by size:
3412 939 552 399
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5302
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5302
NotI GC'GGCC_GC
Cuts at: 2749 2749
Size: 5302
NspI r_CATG'y
Cuts at: 1132 1229 2596 2660 4205 1132
Size: 97 1367 64 1545 2229
Fragments arranged by size:
2229 1545 1367 97 64
PasI CC'CwG_GG
Cuts at: 1348 2244 1348
Size: 896 4406
PciI A'CATG_T
Cuts at: 2592 4201 2592
Size: 1609 3693
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2383 1108
Size: 1275 4027
PmeI GTTT'AAAC
Cuts at: 2606 2606
Size: 5302
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3371 3403 4884 4916 3371
Size: 32 1481 32 3757
Fragments arranged by size:
3757 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5302
PsiI TTA'TAA
Cuts at: 2872 2872
Size: 5302
PspOMI G'GGCC_C
Cuts at: 2197 2386 2197
Size: 189 5113
PstI C_TGCA'G
Cuts at: 830 2302 2654 830
Size: 1472 352 3478
Fragments arranged by size:
3478 1472 352
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5302
PvuII CAG'CTG
Cuts at: 2371 2371
Size: 5302
SalI G'TCGA_C
Cuts at: 1936 2644 1936
Size: 708 4594
SbfI CC_TGCA'GG
Cuts at: 2302 2654 2302
Size: 352 4950
ScaI AGT'ACT
Cuts at: 2028 3550 5262 2028
Size: 1522 1712 2068
Fragments arranged by size:
2068 1712 1522
SfcI C'TryA_G
Cuts at: 826 1045 2231 2298 2650 3785 4466 4657 826
Size: 219 1186 67 352 1135 681 191 1471
Fragments arranged by size:
1471 1186 1135 681 352 219 191 67
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5302
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5302
SmaI CCC'GGG
Cuts at: 1872 2629 1872
Size: 757 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3406 4307 4569 820
Size: 197 926 1463 901 262 1553
Fragments arranged by size:
1553 1463 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3093 493
Size: 2600 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5302
SphI G_CATG'C
Cuts at: 1132 2660 1132
Size: 1528 3774
SspI AAT'ATT
Cuts at: 5 52 3226 5
Size: 47 3174 2081
Fragments arranged by size:
3174 2081 47
StuI AGG'CCT
Cuts at: 1517 2154 2492 1517
Size: 637 338 4327
Fragments arranged by size:
4327 637 338
StyI C'CwwG_G
Cuts at: 513 1065 2004 2403 2741 513
Size: 552 939 399 338 3074
Fragments arranged by size:
3074 939 552 399 338
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2042 2303 3329 3346 3499 3684 4129
Size: 1255 462 261 1026 17 153 185 445
Cuts at: 4129 325
Size: 1498
Fragments arranged by size:
1498 1255 1026 462 445 261 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2026 3548 5260
Size: 274 80 33 51 1443 49 1522 1712
Cuts at: 5260 96
Size: 138
Fragments arranged by size:
1712 1522 1443 274 138 80 51 49
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5262 5294 5262
Size: 32 5270
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5302
XbaI T'CTAG_A
Cuts at: 2638 2638
Size: 5302
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5302
XmaI C'CCGG_G
Cuts at: 1870 2627 1870
Size: 757 4545
XmnI GAAnn'nnTTC
Cuts at: 3431 3431
Size: 5302
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3110 276
Size: 53 83 186 936 1576 2468
Fragments arranged by size:
2468 1576 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI