Restriction map for
pFN21AE1409
(Circular) (Six-base) MAPSORT of: pFN21AE1409.seq Check: 334 from: 1 to: 6109
pFN21AE1409 6109 bp
With 182 enzymes: SgfI *
August 16, 2010 14:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3919 278
Size: 53 83 186 936 2383 2468
Fragments arranged by size:
2468 2383 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3452 1937
Size: 1515 4594
Acc65I G'GTAC_C
Cuts at: 3430 3430
Size: 6109
AclI AA'CG_TT
Cuts at: 1563 4236 4609 1563
Size: 2673 373 3063
Fragments arranged by size:
3063 2673 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3054 4172 5556 5824 1632
Size: 1422 1118 1384 268 1917
Fragments arranged by size:
1917 1422 1384 1118 268
AfeI AGC'GCT
Cuts at: 2166 2166
Size: 6109
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5912
AflIII A'CryG_T
Cuts at: 1203 5008 5985 1203
Size: 3805 977 1327
Fragments arranged by size:
3805 1327 977
AgeI A'CCGG_T
Cuts at: 2442 4940 2442
Size: 2498 3611
AhdI GACnn_n'nnGTC
Cuts at: 2920 4838 2920
Size: 1918 4191
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6109
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2587 1184
Size: 1403 4706
AlwNI CAG_nnn'CTG
Cuts at: 3013 3032 3392 3852 5424 3013
Size: 19 360 460 1572 3698
Fragments arranged by size:
3698 1572 460 360 19
ApaI G_GGCC'C
Cuts at: 2209 2209
Size: 6109
ApaLI G'TGCA_C
Cuts at: 2446 4166 5322 2446
Size: 1720 1156 3233
Fragments arranged by size:
3233 1720 1156
ApoI r'AATT_y
Cuts at: 1493 3418 3644 3796 5905 1493
Size: 1925 226 152 2109 1697
Fragments arranged by size:
2109 1925 1697 226 152
AscI GG'CGCG_CC
Cuts at: 2218 2218
Size: 6109
AseI AT'TA_AT
Cuts at: 160 1032 4663 160
Size: 872 3631 1606
Fragments arranged by size:
3631 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6109
AvaI C'yCGr_G
Cuts at: 1870 1943 2063 2202 2208 3434 1870
Size: 73 120 139 6 1226 4545
Fragments arranged by size:
4545 1226 139 120 73 6
BamHI G'GATC_C
Cuts at: 3439 3806 3439
Size: 367 5742
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2008 2634 3430 4885 618
Size: 325 217 627 221 626 796 1455 1842
Fragments arranged by size:
1842 1455 796 627 626 325 221 217
BanII G_rGCy'C
Cuts at: 1391 2123 2209 3428 1391
Size: 732 86 1219 4072
Fragments arranged by size:
4072 1219 732 86
BbeI G_GCGC'C
Cuts at: 2012 2012
Size: 6109
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5138
BbvCI CC'TCA_GC
Cuts at: 2877 2877
Size: 6109
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4300 4334 1903
Size: 34 2363 34 3678
Fragments arranged by size:
3678 2363 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4001 5211 4001
Size: 1210 4899
BclI T'GATC_A
Cuts at: 1548 2347 1548
Size: 799 5310
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4720 136
Size: 107 122 71 631 605 3048 1525
Fragments arranged by size:
3048 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6104 6104
Size: 6109
BlpI GC'TnA_GC
Cuts at: 2323 3526 2323
Size: 1203 4906
Bme1580I G_kGCm'C
Cuts at: 1790 2203 2209 2450 2639 3132 4170 5326 1790
Size: 413 6 241 189 493 1038 1156 2573
Fragments arranged by size:
2573 1156 1038 493 413 241 189 6
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6109
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2190 3840 4798
Size: 416 53 83 399 24 773 1650 958
Cuts at: 4798 442
Size: 1753
Fragments arranged by size:
1753 1650 958 773 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6109
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2183 2183
Size: 6109
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2451 2695 4769 1239
Size: 1212 244 2074 2579
Fragments arranged by size:
2579 2074 1212 244
Bpu10I CC'TnA_GC
Cuts at: 2800 2877 2800
Size: 77 6032
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4234 5099 5397 4234
Size: 865 298 4946
Fragments arranged by size:
4946 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2861 3045 4772 882
Size: 617 1362 184 1727 2219
Fragments arranged by size:
2219 1727 1362 617 184
BsaAI yAC'GTr
Cuts at: 493 1204 3900 493
Size: 711 2696 2702
Fragments arranged by size:
2702 2696 711
BsaBI GATnn'nnATC
Cuts at: 6103 6103
Size: 6109
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2009 3916 4298 4933
Size: 53 83 186 936 476 1907 382 635
Cuts at: 4933 275
Size: 1451
Fragments arranged by size:
1907 1451 936 635 476 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2442 2589 4542 4940 5214 5361 5772 2442
Size: 147 1953 398 274 147 411 2779
Fragments arranged by size:
2779 1953 411 398 274 147 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2993 3023 1178
Size: 30 165 30 1590 30 4264
Fragments arranged by size:
4264 1590 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2175 3061 3064 1186
Size: 989 886 3 4231
Fragments arranged by size:
4231 989 886 3
BseYI C'CCAG_C
Cuts at: 2105 3016 5312 5808 2105
Size: 911 2296 496 2406
Fragments arranged by size:
2406 2296 911 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2527 2644 1163
Size: 573 791 117 4628
Fragments arranged by size:
4628 791 573 117
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3559 4320 5348 1540
Size: 275 186 1558 761 1028 2301
Fragments arranged by size:
2301 1558 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2450 4170 4255 5326 2450
Size: 1720 85 1071 3233
Fragments arranged by size:
3233 1720 1071 85
BsmI GAATG_Cn'
Cuts at: 3620 3713 3620
Size: 93 6016
BsmBI CGTCTCn'nnnn_
Cuts at: 2938 2938
Size: 6109
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2123 2203 2209 2450 2639 3132 3428
Size: 399 333 80 6 241 189 493 296
Cuts at: 3428 4170 4255 5326 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 493 399 333 296 241
189 85 80 6
BspEI T'CCGG_A
Cuts at: 2589 5772 2589
Size: 3183 2926
BspHI T'CATG_A
Cuts at: 2344 3153 3998 5728 2344
Size: 809 845 1730 2725
Fragments arranged by size:
2725 1730 845 809
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2299 3464 844
Size: 1455 1165 3489
Fragments arranged by size:
3489 1455 1165
BsrBI CCG'CTC
Cuts at: 1394 1823 3996 1394
Size: 429 2173 3507
Fragments arranged by size:
3507 2173 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2375 2716 3240 4604 4778 65
Size: 1183 1127 341 524 1364 174 1396
Fragments arranged by size:
1396 1364 1183 1127 524 341 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2236 2442 2962 3022 4753 4940 1700
Size: 110 426 206 520 60 1731 187 2869
Fragments arranged by size:
2869 1731 520 426 206 187 110 60
BsrGI T'GTAC_A
Cuts at: 96 2504 2986 96
Size: 2408 482 3219
Fragments arranged by size:
3219 2408 482
BssHII G'CGCG_C
Cuts at: 1925 2218 1925
Size: 293 5816
BssSI C'ACGA_G
Cuts at: 2145 4169 5181 2145
Size: 2024 1012 3073
Fragments arranged by size:
3073 2024 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2634 3074 794
Size: 1840 440 3829
Fragments arranged by size:
3829 1840 440
BstBI TT'CG_AA
Cuts at: 4913 4913
Size: 6109
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6109
BstXI CCAn_nnnn'nTGG
Cuts at: 2647 2827 2647
Size: 180 5929
BstYI r'GATC_y
Cuts at: 1972 2408 3439 3806 4191 4208 5660 5746 5758
Size: 436 1031 367 385 17 1452 86 12
Cuts at: 5758 6104 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1031 436 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2094 2127 2178 513
Size: 552 939 90 33 51 4444
Fragments arranged by size:
4444 939 552 90 51 33
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5370
Fragments arranged by size:
5370 683 56
BtsI GCAGTG_nn'
Cuts at: 2055 3625 4419 4439 2055
Size: 1570 794 20 3725
Fragments arranged by size:
3725 1570 794 20
ClaI AT'CG_AT
Cuts at: 1554 3186 1554
Size: 1632 4477
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6074
DraI TTT'AAA
Cuts at: 3413 3413
Size: 6109
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5116 809
Size: 507 3800 1802
Fragments arranged by size:
3800 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2097 3556 4445
Size: 54 1425 50 275 18 267 1459 889
Cuts at: 4445 6074 8
Size: 1629 43
Fragments arranged by size:
1629 1459 1425 889 275 267 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3556 1537
Size: 275 1744 4090
Fragments arranged by size:
4090 1744 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2128 2523 3306 4039 1353
Size: 775 395 783 733 3423
Fragments arranged by size:
3423 783 775 733 395
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4696 5070 5216 387
Size: 1064 138 3107 374 146 1280
Fragments arranged by size:
3107 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2451 2695 3054 4172 4769 5556 5824
Size: 393 819 244 359 1118 597 787 268
Cuts at: 5824 1239
Size: 1524
Fragments arranged by size:
1524 1118 819 787 597 393 359 268
244
EcoNI CCTnn'n_nnAGG
Cuts at: 2306 3301 2306
Size: 995 5114
EcoO109I rG'GnC_Cy
Cuts at: 2228 2299 2815 2867 3340 2228
Size: 71 516 52 473 4997
Fragments arranged by size:
4997 516 473 71 52
EcoRI G'AATT_C
Cuts at: 3418 3796 3418
Size: 378 5731
FspI TGC'GCA
Cuts at: 4615 4615
Size: 6109
HaeII r_GCGC'y
Cuts at: 1678 2012 2168 2851 5256 1678
Size: 334 156 683 2405 2531
Fragments arranged by size:
2531 2405 683 334 156
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2044 2076 2634 2666 2906
Size: 32 135 32 472 32 558 32 240
Cuts at: 2906 2938 4750 4782 4824 4856 1373
Size: 32 1812 32 42 32 2626
Fragments arranged by size:
2626 1812 558 472 240 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3253 3453 3699 669
Size: 988 281 1315 200 246 3079
Fragments arranged by size:
3079 1315 988 281 246 200
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6109
HpaI GTT'AAC
Cuts at: 3699 3699
Size: 6109
KasI G'GCGC_C
Cuts at: 2008 2008
Size: 6109
KpnI G_GTAC'C
Cuts at: 3434 3434
Size: 6109
MfeI C'AATT_G
Cuts at: 2355 3708 2355
Size: 1353 4756
MluI A'CGCG_T
Cuts at: 5985 5985
Size: 6109
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2502 5223 5407 1305
Size: 1197 2721 184 2007
Fragments arranged by size:
2721 2007 1197 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4287
Fragments arranged by size:
4287 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2034 2368 2569 4067 4426 4585 518
Size: 648 868 334 201 1498 359 159 2042
Fragments arranged by size:
2042 1498 868 648 359 334 201 159
MspA1I CmG'CkG
Cuts at: 1606 2096 2129 2327 3163 3525 4202 5350 5595
Size: 490 33 198 836 362 677 1148 245
Cuts at: 5595 1606
Size: 2120
Fragments arranged by size:
2120 1148 836 677 490 362 245 198
33
NaeI GCC'GGC
Cuts at: 2238 2964 2238
Size: 726 5383
NarI GG'CG_CC
Cuts at: 2009 2009
Size: 6109
NcoI C'CATG_G
Cuts at: 513 1065 2004 2178 513
Size: 552 939 174 4444
Fragments arranged by size:
4444 939 552 174
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6109
NgoMIV G'CCGG_C
Cuts at: 2236 2962 2236
Size: 726 5383
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6109
NotI GC'GGCC_GC
Cuts at: 3556 3556
Size: 6109
NspI r_CATG'y
Cuts at: 1132 1229 2568 3323 3467 5012 1132
Size: 97 1339 755 144 1545 2229
Fragments arranged by size:
2229 1545 1339 755 144 97
PasI CC'CwG_GG
Cuts at: 1348 2996 3215 1348
Size: 1648 219 4242
Fragments arranged by size:
4242 1648 219
PciI A'CATG_T
Cuts at: 5008 5008
Size: 6109
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3071 1108
Size: 1963 4146
PfoI T'CCnGG_A
Cuts at: 2428 2428
Size: 6109
PmeI GTTT'AAAC
Cuts at: 3413 3413
Size: 6109
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4178 4210 5691 5723 4178
Size: 32 1481 32 4564
Fragments arranged by size:
4564 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2228 2299 2815 3340 2228
Size: 71 516 525 4997
Fragments arranged by size:
4997 525 516 71
PshAI GACnn'nnGTC
Cuts at: 1613 2978 1613
Size: 1365 4744
PsiI TTA'TAA
Cuts at: 3679 3679
Size: 6109
PspOMI G'GGCC_C
Cuts at: 2205 2205
Size: 6109
PstI C_TGCA'G
Cuts at: 830 2310 3461 830
Size: 1480 1151 3478
Fragments arranged by size:
3478 1480 1151
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6109
PvuII CAG'CTG
Cuts at: 2327 3163 2327
Size: 836 5273
SacII CC_GC'GG
Cuts at: 2097 2130 2097
Size: 33 6076
SalI G'TCGA_C
Cuts at: 1936 3451 1936
Size: 1515 4594
SanDI GG'GwC_CC
Cuts at: 2815 2815
Size: 6109
SapI GCTCTTCn'nnn_
Cuts at: 2128 2128
Size: 6109
SbfI CC_TGCA'GG
Cuts at: 3461 3461
Size: 6109
ScaI AGT'ACT
Cuts at: 4357 6069 4357
Size: 1712 4397
SfcI C'TryA_G
Cuts at: 826 1045 2306 3457 4592 5273 5464 826
Size: 219 1261 1151 1135 681 191 1471
Fragments arranged by size:
1471 1261 1151 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2010 2010
Size: 6109
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6109
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6109
SmaI CCC'GGG
Cuts at: 1872 2065 2204 2210 3436 1872
Size: 193 139 6 1226 4545
Fragments arranged by size:
4545 1226 193 139 6
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4213 5114 5376 820
Size: 197 926 2270 901 262 1553
Fragments arranged by size:
2270 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3900 493
Size: 3407 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6109
SphI G_CATG'C
Cuts at: 1132 3467 1132
Size: 2335 3774
SrfI GCCC'GGGC
Cuts at: 2204 2210 2204
Size: 6 6103
SspI AAT'ATT
Cuts at: 5 52 4033 5
Size: 47 3981 2081
Fragments arranged by size:
3981 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6109
StyI C'CwwG_G
Cuts at: 513 1065 2004 2178 3297 3402 3548 513
Size: 552 939 174 1119 105 146 3074
Fragments arranged by size:
3074 1119 939 552 174 146 105
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2119 2716 4136 4153 4306 4491 4936
Size: 1255 539 597 1420 17 153 185 445
Cuts at: 4936 325
Size: 1498
Fragments arranged by size:
1498 1420 1255 597 539 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2026 2504 2986
Size: 274 80 33 51 1443 49 478 482
Cuts at: 2986 4355 6067 96
Size: 1369 1712 138
Fragments arranged by size:
1712 1443 1369 482 478 274 138 80
51 49 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2807 2822 2839 2854 2993 3025 6069 6101 2807
Size: 15 17 15 139 32 3044 32 2815
Fragments arranged by size:
3044 2815 139 32 32 17 15 15
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6109
XbaI T'CTAG_A
Cuts at: 3445 3445
Size: 6109
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6109
XmaI C'CCGG_G
Cuts at: 1870 2063 2202 2208 3434 1870
Size: 193 139 6 1226 4545
Fragments arranged by size:
4545 1226 193 139 6
XmnI GAAnn'nnTTC
Cuts at: 4238 4238
Size: 6109
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3917 276
Size: 53 83 186 936 2383 2468
Fragments arranged by size:
2468 2383 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI AvrII BaeI BfrBI BsiWI BstZ17I Bsu36I
DraIII EcoICRI EcoRV FalI FseI FspAI NruI NsiI
PacI PmlI PspXI PsrI RsrII SacI SexAI SfiI
SwaI XcmI