Restriction map for
pFN21AE1406
(Circular) (Six-base) MAPSORT of: pFN21AE1406.seq Check: 3626 from: 1 to: 6421
pFN21AE1406 6421 bp
With 182 enzymes: SgfI *
July 6, 2012 17:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2109 4231 278
Size: 53 83 186 936 573 2122 2468
Fragments arranged by size:
2468 2122 936 573 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2109 2502 2523 3764 1937
Size: 172 393 21 1241 4594
Fragments arranged by size:
4594 1241 393 172 21
Acc65I G'GTAC_C
Cuts at: 3520 3742 3520
Size: 222 6199
AclI AA'CG_TT
Cuts at: 1563 4548 4921 1563
Size: 2985 373 3063
Fragments arranged by size:
3063 2985 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2709 3540 3604 4484 5868 6136 1632
Size: 1077 831 64 880 1384 268 1917
Fragments arranged by size:
1917 1384 1077 880 831 268 64
AflII C'TTAA_G
Cuts at: 820 1017 2567 820
Size: 197 1550 4674
Fragments arranged by size:
4674 1550 197
AflIII A'CryG_T
Cuts at: 1203 2223 5320 6297 1203
Size: 1020 3097 977 1327
Fragments arranged by size:
3097 1327 1020 977
AgeI A'CCGG_T
Cuts at: 5252 5252
Size: 6421
AhdI GACnn_n'nnGTC
Cuts at: 5150 5150
Size: 6421
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6421
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6421
AlwNI CAG_nnn'CTG
Cuts at: 4164 5736 4164
Size: 1572 4849
ApaI G_GGCC'C
Cuts at: 2820 2820
Size: 6421
ApaLI G'TGCA_C
Cuts at: 4478 5634 4478
Size: 1156 5265
ApoI r'AATT_y
Cuts at: 1493 3730 3956 4108 6217 1493
Size: 2237 226 152 2109 1697
Fragments arranged by size:
2237 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4975 160
Size: 872 3943 1606
Fragments arranged by size:
3943 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6421
AvaI C'yCGr_G
Cuts at: 1870 1943 2663 3059 3746 1870
Size: 73 720 396 687 4545
Fragments arranged by size:
4545 720 687 396 73
BamHI G'GATC_C
Cuts at: 2637 3751 4118 2637
Size: 1114 367 4940
Fragments arranged by size:
4940 1114 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2864 3520 3632 3682 3742
Size: 325 217 627 1077 656 112 50 60
Cuts at: 3742 5197 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1077 656 627 325 217 112
60 50
BanII G_rGCy'C
Cuts at: 1391 2820 2838 2930 3218 3740 1391
Size: 1429 18 92 288 522 4072
Fragments arranged by size:
4072 1429 522 288 92 18
BbeI G_GCGC'C
Cuts at: 3686 3686
Size: 6421
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2177 3263 3375 928
Size: 971 278 1086 112 3974
Fragments arranged by size:
3974 1086 971 278 112
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2654 2688 4612 4646 1903
Size: 34 717 34 1924 34 3678
Fragments arranged by size:
3678 1924 717 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4313 5523 4313
Size: 1210 5211
BclI T'GATC_A
Cuts at: 1548 2313 1548
Size: 765 5656
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3026 5032 136
Size: 107 122 71 631 605 1354 2006 1525
Fragments arranged by size:
2006 1525 1354 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3174 6416 3174
Size: 3242 3179
BlpI GC'TnA_GC
Cuts at: 3838 3838
Size: 6421
Bme1580I G_kGCm'C
Cuts at: 1790 2820 3637 4482 5638 1790
Size: 1030 817 845 1156 2573
Fragments arranged by size:
2573 1156 1030 845 817
BmgBI CAC'GTC
Cuts at: 1318 3229 1318
Size: 1911 4510
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2896 4152 5110
Size: 416 53 83 399 24 1479 1256 958
Cuts at: 5110 442
Size: 1753
Fragments arranged by size:
1753 1479 1256 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6421
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2135 2480 2663 2717 2896 5081 1239
Size: 896 345 183 54 179 2185 2579
Fragments arranged by size:
2579 2185 896 345 183 179 54
Bpu10I CC'TnA_GC
Cuts at: 2622 2678 2924 2622
Size: 56 246 6119
Fragments arranged by size:
6119 246 56
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4546 5411 5709 4546
Size: 865 298 5258
Fragments arranged by size:
5258 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5084 882
Size: 617 3585 2219
Fragments arranged by size:
3585 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2226 2419 4212 493
Size: 711 1022 193 1793 2702
Fragments arranged by size:
2702 1793 1022 711 193
BsaBI GATnn'nnATC
Cuts at: 2651 6415 2651
Size: 3764 2657
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2106 2484 3683 4228
Size: 53 83 186 936 573 378 1199 545
Cuts at: 4228 4610 5245 275
Size: 382 635 1451
Fragments arranged by size:
1451 1199 936 635 573 545 382 378
186 83 53
BsaWI w'CCGG_w
Cuts at: 4854 5252 5526 5673 6084 4854
Size: 398 274 147 411 5191
Fragments arranged by size:
5191 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2902 2932 2942 2972 3422
Size: 30 165 30 1499 30 10 30 450
Cuts at: 3422 3452 3535 3565 1178
Size: 30 83 30 4034
Fragments arranged by size:
4034 1499 450 165 83 30 30 30
30 30 30 10
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2045 2468 3095 1186
Size: 859 423 627 4512
Fragments arranged by size:
4512 859 627 423
BseYI C'CCAG_C
Cuts at: 5624 6120 5624
Size: 496 5925
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2492 2802 1163
Size: 573 756 310 4782
Fragments arranged by size:
4782 756 573 310
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3096 3871 4632 5660 1540
Size: 275 186 1095 775 761 1028 2301
Fragments arranged by size:
2301 1095 1028 775 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3435 4482 4567 5638 3435
Size: 1047 85 1071 4218
Fragments arranged by size:
4218 1071 1047 85
BsmI GAATG_Cn'
Cuts at: 3932 4025 3932
Size: 93 6328
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2820 2838 2930 3218 3435 3637 3740
Size: 399 1030 18 92 288 217 202 103
Cuts at: 3740 4482 4567 5638 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 1030 742 399 288 217 202
103 92 85 18
BspEI T'CCGG_A
Cuts at: 6084 6084
Size: 6421
BspHI T'CATG_A
Cuts at: 2769 4310 6040 2769
Size: 1541 1730 3150
Fragments arranged by size:
3150 1730 1541
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2238 2974 3776 844
Size: 1394 736 802 3489
Fragments arranged by size:
3489 1394 802 736
BsrBI CCG'CTC
Cuts at: 1394 1823 3085 3551 3699 4308 1394
Size: 429 1262 466 148 609 3507
Fragments arranged by size:
3507 1262 609 466 429 148
BsrDI GCAATG_nn'
Cuts at: 65 1248 2727 2838 4916 5090 65
Size: 1183 1479 111 2078 174 1396
Fragments arranged by size:
2078 1479 1396 1183 174 111
BsrFI r'CCGG_y
Cuts at: 1700 1810 3665 5065 5252 1700
Size: 110 1855 1400 187 2869
Fragments arranged by size:
2869 1855 1400 187 110
BsrGI T'GTAC_A
Cuts at: 96 2754 96
Size: 2658 3763
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6421
BssSI C'ACGA_G
Cuts at: 2041 4481 5493 2041
Size: 2440 1012 2969
Fragments arranged by size:
2969 2440 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6421
BstBI TT'CG_AA
Cuts at: 5225 5225
Size: 6421
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6421
BstYI r'GATC_y
Cuts at: 1972 2637 3174 3751 4118 4503 4520 5972 6058
Size: 665 537 577 367 385 17 1452 86
Cuts at: 6058 6070 6416 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 665 577 537 385 367 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 3210 3322 3413 513
Size: 552 2145 112 91 3521
Fragments arranged by size:
3521 2145 552 112 91
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2779 487
Size: 683 56 1553 4129
Fragments arranged by size:
4129 1553 683 56
BtsI GCAGTG_nn'
Cuts at: 3015 3937 4731 4751 3015
Size: 922 794 20 4685
Fragments arranged by size:
4685 922 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6421
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6386
DraI TTT'AAA
Cuts at: 3725 3725
Size: 6421
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2483 2984 5428 809
Size: 507 1167 501 2444 1802
Fragments arranged by size:
2444 1802 1167 507 501
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2802 2998 3667
Size: 54 1425 50 275 18 972 196 669
Cuts at: 3667 3694 3868 4757 6386 8
Size: 27 174 889 1629 43
Fragments arranged by size:
1629 1425 972 889 669 275 196 174
54 50 43 27 18
EagI C'GGCC_G
Cuts at: 1537 1812 3868 1537
Size: 275 2056 4090
Fragments arranged by size:
4090 2056 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2018 2685 4351 1353
Size: 665 667 1666 3423
Fragments arranged by size:
3423 1666 667 665
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2206 3217 5008 5382 5528 387
Size: 1064 138 617 1011 1791 374 146 1280
Fragments arranged by size:
1791 1280 1064 1011 617 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2135 2480 2663 2709 2717 2896 3540
Size: 393 503 345 183 46 8 179 644
Cuts at: 3540 3604 4484 5081 5868 6136 1239
Size: 64 880 597 787 268 1524
Fragments arranged by size:
1524 880 787 644 597 503 393 345
268 183 179 64 46 8
EcoO109I rG'GnC_Cy
Cuts at: 2572 2660 2816 2817 3241 2572
Size: 88 156 1 424 5752
Fragments arranged by size:
5752 424 156 88 1
EcoRI G'AATT_C
Cuts at: 3730 4108 3730
Size: 378 6043
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3061 3379 3061
Size: 318 6103
FspI TGC'GCA
Cuts at: 4927 4927
Size: 6421
HaeII r_GCGC'y
Cuts at: 1678 3686 5568 1678
Size: 2008 1882 2531
Fragments arranged by size:
2531 2008 1882
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2043 2061 2075 2093 2107
Size: 32 135 32 471 18 14 18 14
Cuts at: 2107 2378 2410 2571 2603 2902 2934 5062 5094
Size: 271 32 161 32 299 32 2128 32
Cuts at: 5094 5136 5168 1373
Size: 42 32 2626
Fragments arranged by size:
2626 2128 471 299 271 161 135 42
32 32 32 32 32 32 32 18
18 14 14
HincII GTy'rAC
Cuts at: 669 1657 1938 2221 3070 3388 3765 4011 669
Size: 988 281 283 849 318 377 246 3079
Fragments arranged by size:
3079 988 849 377 318 283 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6421
HpaI GTT'AAC
Cuts at: 4011 4011
Size: 6421
KasI G'GCGC_C
Cuts at: 3682 3682
Size: 6421
KpnI G_GTAC'C
Cuts at: 3524 3746 3524
Size: 222 6199
MfeI C'AATT_G
Cuts at: 4020 4020
Size: 6421
MluI A'CGCG_T
Cuts at: 6297 6297
Size: 6421
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2559 3639 3726 5535 5719 1305
Size: 1254 1080 87 1809 184 2007
Fragments arranged by size:
2007 1809 1254 1080 184 87
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2804 10
Size: 54 1425 343 972 3627
Fragments arranged by size:
3627 1425 972 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4379 4738 4897 518
Size: 648 3213 359 159 2042
Fragments arranged by size:
3213 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2384 2997 3415 3837 4514 5662 5907 1606
Size: 778 613 418 422 677 1148 245 2120
Fragments arranged by size:
2120 1148 778 677 613 422 418 245
NarI GG'CG_CC
Cuts at: 3683 3683
Size: 6421
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5869
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6421
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6421
NotI GC'GGCC_GC
Cuts at: 3868 3868
Size: 6421
NspI r_CATG'y
Cuts at: 1132 1229 3499 3779 5324 1132
Size: 97 2270 280 1545 2229
Fragments arranged by size:
2270 2229 1545 280 97
PasI CC'CwG_GG
Cuts at: 1348 2245 1348
Size: 897 5524
PciI A'CATG_T
Cuts at: 5320 5320
Size: 6421
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6421
PfoI T'CCnGG_A
Cuts at: 2203 2640 3426 2203
Size: 437 786 5198
Fragments arranged by size:
5198 786 437
PmeI GTTT'AAAC
Cuts at: 3725 3725
Size: 6421
PmlI CAC'GTG
Cuts at: 2419 2419
Size: 6421
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2696 2728 4490 4522 6003 6035 2696
Size: 32 1762 32 1481 32 3082
Fragments arranged by size:
3082 1762 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2660 3241 2660
Size: 581 5840
PshAI GACnn'nnGTC
Cuts at: 1613 3095 1613
Size: 1482 4939
PsiI TTA'TAA
Cuts at: 3991 3991
Size: 6421
PspOMI G'GGCC_C
Cuts at: 2816 2816
Size: 6421
PstI C_TGCA'G
Cuts at: 830 2235 3773 830
Size: 1405 1538 3478
Fragments arranged by size:
3478 1538 1405
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6421
PvuII CAG'CTG
Cuts at: 2384 2384
Size: 6421
SacII CC_GC'GG
Cuts at: 3416 3416
Size: 6421
SalI G'TCGA_C
Cuts at: 1936 3763 1936
Size: 1827 4594
SanDI GG'GwC_CC
Cuts at: 2660 3241 2660
Size: 581 5840
SapI GCTCTTCn'nnn_
Cuts at: 2018 2685 2018
Size: 667 5754
SbfI CC_TGCA'GG
Cuts at: 3773 3773
Size: 6421
ScaI AGT'ACT
Cuts at: 4669 6381 4669
Size: 1712 4709
SfcI C'TryA_G
Cuts at: 826 1045 2231 3010 3769 4904 5585 5776 826
Size: 219 1186 779 759 1135 681 191 1471
Fragments arranged by size:
1471 1186 1135 779 759 681 219 191
SfoI GGC'GCC
Cuts at: 3684 3684
Size: 6421
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6421
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6421
SmaI CCC'GGG
Cuts at: 1872 3748 1872
Size: 1876 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2567 4525 5426 5688 820
Size: 197 926 624 1958 901 262 1553
Fragments arranged by size:
1958 1553 926 901 624 262 197
SnaBI TAC'GTA
Cuts at: 493 4212 493
Size: 3719 2702
SpeI A'CTAG_T
Cuts at: 152 3334 152
Size: 3182 3239
SphI G_CATG'C
Cuts at: 1132 3779 1132
Size: 2647 3774
SspI AAT'ATT
Cuts at: 5 52 4345 5
Size: 47 4293 2081
Fragments arranged by size:
4293 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6421
StyI C'CwwG_G
Cuts at: 513 1065 2275 2763 2892 3191 3860 513
Size: 552 1210 488 129 299 669 3074
Fragments arranged by size:
3074 1210 669 552 488 299 129
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2064 2873 2882 3547 3607 4448 4465
Size: 1255 484 809 9 665 60 841 17
Cuts at: 4465 4618 4803 5248 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 841 809 665 484 445 185
153 60 17 9
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2754 3294 4667
Size: 274 80 33 51 1443 777 540 1373
Cuts at: 4667 6379 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1373 777 540 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6381 6413 6381
Size: 32 6389
Tth111I GACn'n_nGTC
Cuts at: 1316 3230 1316
Size: 1914 4507
XbaI T'CTAG_A
Cuts at: 3757 3757
Size: 6421
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2191 2290 2603 2191
Size: 99 313 6009
Fragments arranged by size:
6009 313 99
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6421
XmaI C'CCGG_G
Cuts at: 1870 3746 1870
Size: 1876 4545
XmnI GAAnn'nnTTC
Cuts at: 3422 4550 3422
Size: 1128 5293
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2107 4229 276
Size: 53 83 186 936 573 2122 2468
Fragments arranged by size:
2468 2122 936 573 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI
PacI PspXI PsrI RsrII SacI SexAI SfiI SrfI
SwaI