Restriction map for
pFN21AE1404
(Circular) (Six-base) MAPSORT of: pFN21AE1404.seq Check: 6615 from: 1 to: 5719
pFN21AE1404 5719 bp
With 182 enzymes: SgfI *
August 16, 2010 14:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3529 278
Size: 53 83 186 936 1993 2468
Fragments arranged by size:
2468 1993 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2989 3062 1937
Size: 1052 73 4594
Fragments arranged by size:
4594 1052 73
Acc65I G'GTAC_C
Cuts at: 2048 3040 2048
Size: 992 4727
AclI AA'CG_TT
Cuts at: 1563 3846 4219 1563
Size: 2283 373 3063
Fragments arranged by size:
3063 2283 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2378 3782 5166 5434 1632
Size: 746 1404 1384 268 1917
Fragments arranged by size:
1917 1404 1384 746 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5522
AflIII A'CryG_T
Cuts at: 1203 2992 4618 5595 1203
Size: 1789 1626 977 1327
Fragments arranged by size:
1789 1626 1327 977
AgeI A'CCGG_T
Cuts at: 4550 4550
Size: 5719
AhdI GACnn_n'nnGTC
Cuts at: 4448 4448
Size: 5719
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5719
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5719
AlwNI CAG_nnn'CTG
Cuts at: 2396 2704 3462 5034 2396
Size: 308 758 1572 3081
Fragments arranged by size:
3081 1572 758 308
ApaI G_GGCC'C
Cuts at: 2405 2405
Size: 5719
ApaLI G'TGCA_C
Cuts at: 2115 3776 4932 2115
Size: 1661 1156 2902
Fragments arranged by size:
2902 1661 1156
ApoI r'AATT_y
Cuts at: 1493 2749 3028 3254 3406 5515 1493
Size: 1256 279 226 152 2109 1697
Fragments arranged by size:
2109 1697 1256 279 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4273 160
Size: 872 3241 1606
Fragments arranged by size:
3241 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5719
AvaI C'yCGr_G
Cuts at: 1870 1943 3044 1870
Size: 73 1101 4545
Fragments arranged by size:
4545 1101 73
BamHI G'GATC_C
Cuts at: 3049 3416 3049
Size: 367 5352
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2048 2194 3040 4495 618
Size: 325 217 627 261 146 846 1455 1842
Fragments arranged by size:
1842 1455 846 627 325 261 217 146
BanII G_rGCy'C
Cuts at: 1391 2405 2465 3038 1391
Size: 1014 60 573 4072
Fragments arranged by size:
4072 1014 573 60
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2957 928
Size: 971 1058 3690
Fragments arranged by size:
3690 1058 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3910 3944 1903
Size: 34 1973 34 3678
Fragments arranged by size:
3678 1973 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3611 4821 3611
Size: 1210 4509
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5719
BfrBI ATG'CAT
Cuts at: 2693 2693
Size: 5719
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4330 136
Size: 107 122 71 631 605 2658 1525
Fragments arranged by size:
2658 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5714 5714
Size: 5719
BlpI GC'TnA_GC
Cuts at: 3136 3136
Size: 5719
Bme1580I G_kGCm'C
Cuts at: 1790 2119 2405 3780 4936 1790
Size: 329 286 1375 1156 2573
Fragments arranged by size:
2573 1375 1156 329 286
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5719
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3450 4408 442
Size: 416 53 83 399 24 2033 958 1753
Fragments arranged by size:
2033 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5719
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2441 2486 2867 4379 1239
Size: 1202 45 381 1512 2579
Fragments arranged by size:
2579 1512 1202 381 45
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2596 2882 3844 4709 5007 2596
Size: 286 962 865 298 3308
Fragments arranged by size:
3308 962 865 298 286
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2615 4382 882
Size: 617 1116 1767 2219
Fragments arranged by size:
2219 1767 1116 617
BsaAI yAC'GTr
Cuts at: 493 1204 2993 3510 493
Size: 711 1789 517 2702
Fragments arranged by size:
2702 1789 711 517
BsaBI GATnn'nnATC
Cuts at: 2943 5713 2943
Size: 2770 2949
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3526 3908 4543 275
Size: 53 83 186 936 1993 382 635 1451
Fragments arranged by size:
1993 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2971 4152 4550 4824 4971 5382 2971
Size: 1181 398 274 147 411 3308
Fragments arranged by size:
3308 1181 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5494
Fragments arranged by size:
5494 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5719
BseYI C'CCAG_C
Cuts at: 2458 4922 5418 2458
Size: 2464 496 2759
Fragments arranged by size:
2759 2464 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5146
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3169 3930 4958 1540
Size: 275 186 1168 761 1028 2301
Fragments arranged by size:
2301 1168 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2119 3780 3865 4936 2119
Size: 1661 85 1071 2902
Fragments arranged by size:
2902 1661 1071 85
BsmI GAATG_Cn'
Cuts at: 3230 3323 3230
Size: 93 5626
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2119 2405 2465 3038 3780 3865 4936
Size: 399 329 286 60 573 742 85 1071
Cuts at: 4936 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 573 399 329 286 85
60
BspEI T'CCGG_A
Cuts at: 5382 5382
Size: 5719
BspHI T'CATG_A
Cuts at: 3608 5338 3608
Size: 1730 3989
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3074 844
Size: 2230 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3606 1394
Size: 429 1783 3507
Fragments arranged by size:
3507 1783 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2693 4214 4388 65
Size: 1183 1445 1521 174 1396
Fragments arranged by size:
1521 1445 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4363 4550 1700
Size: 110 2553 187 2869
Fragments arranged by size:
2869 2553 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5719
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5719
BssSI C'ACGA_G
Cuts at: 3779 4791 3779
Size: 1012 4707
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2704 794
Size: 1910 3809
BstBI TT'CG_AA
Cuts at: 4523 4523
Size: 5719
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5719
BstXI CCAn_nnnn'nTGG
Cuts at: 2419 2419
Size: 5719
BstYI r'GATC_y
Cuts at: 1972 2425 2782 2892 3049 3416 3801 3818 5270
Size: 453 357 110 157 367 385 17 1452
Cuts at: 5270 5356 5368 5714 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 453 385 367 357 346 157
110 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2222 2222
Size: 5719
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5167
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4980
Fragments arranged by size:
4980 683 56
BtsI GCAGTG_nn'
Cuts at: 2288 3235 4029 4049 2288
Size: 947 794 20 3958
Fragments arranged by size:
3958 947 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5719
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5684
DraI TTT'AAA
Cuts at: 2545 3023 2545
Size: 478 5241
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4726 809
Size: 507 3410 1802
Fragments arranged by size:
3410 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2056 3166 4055
Size: 54 1425 50 275 18 226 1110 889
Cuts at: 4055 5684 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1110 889 275 226 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3166 1537
Size: 275 1354 4090
Fragments arranged by size:
4090 1354 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2033 2628 3649 1353
Size: 680 595 1021 3423
Fragments arranged by size:
3423 1021 680 595
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4306 4680 4826 387
Size: 1064 138 2717 374 146 1280
Fragments arranged by size:
2717 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2378 2441 2486 2867 3782 4379 5166
Size: 393 746 63 45 381 915 597 787
Cuts at: 5166 5434 1239
Size: 268 1524
Fragments arranged by size:
1524 915 787 746 597 393 381 268
63 45
EcoRI G'AATT_C
Cuts at: 3028 3406 3028
Size: 378 5341
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3029 3029
Size: 5719
FspI TGC'GCA
Cuts at: 4225 4225
Size: 5719
HaeII r_GCGC'y
Cuts at: 1678 4866 1678
Size: 3188 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2569 2601 4360 4392 4434
Size: 32 135 32 997 32 1759 32 42
Cuts at: 4434 4466 1373
Size: 32 2626
Fragments arranged by size:
2626 1759 997 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3063 3309 669
Size: 988 281 1125 246 3079
Fragments arranged by size:
3079 1125 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2798 748
Size: 2050 3669
HpaI GTT'AAC
Cuts at: 3309 3309
Size: 5719
KpnI G_GTAC'C
Cuts at: 2052 3044 2052
Size: 992 4727
MfeI C'AATT_G
Cuts at: 3318 3318
Size: 5719
MluI A'CGCG_T
Cuts at: 5595 5595
Size: 5719
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4833 5017 1305
Size: 3528 184 2007
Fragments arranged by size:
3528 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2058 10
Size: 54 1425 343 226 3671
Fragments arranged by size:
3671 1425 343 226 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2122 2247 3677 4036 4195 518
Size: 648 956 125 1430 359 159 2042
Fragments arranged by size:
2042 1430 956 648 359 159 125
MspA1I CmG'CkG
Cuts at: 1606 2537 3135 3812 4960 5205 1606
Size: 931 598 677 1148 245 2120
Fragments arranged by size:
2120 1148 931 677 598 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5167
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5719
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5719
NotI GC'GGCC_GC
Cuts at: 3166 3166
Size: 5719
NsiI A_TGCA'T
Cuts at: 2695 2695
Size: 5719
NspI r_CATG'y
Cuts at: 1132 1229 3077 4622 1132
Size: 97 1848 1545 2229
Fragments arranged by size:
2229 1848 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5719
PciI A'CATG_T
Cuts at: 4618 4618
Size: 5719
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5719
PfoI T'CCnGG_A
Cuts at: 2200 2200
Size: 5719
PmeI GTTT'AAAC
Cuts at: 3023 3023
Size: 5719
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3788 3820 5301 5333 3788
Size: 32 1481 32 4174
Fragments arranged by size:
4174 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5719
PsiI TTA'TAA
Cuts at: 3289 3289
Size: 5719
PspOMI G'GGCC_C
Cuts at: 2401 2401
Size: 5719
PstI C_TGCA'G
Cuts at: 830 2283 2433 3071 830
Size: 1453 150 638 3478
Fragments arranged by size:
3478 1453 638 150
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5719
PvuII CAG'CTG
Cuts at: 2537 2537
Size: 5719
SalI G'TCGA_C
Cuts at: 1936 3061 1936
Size: 1125 4594
SbfI CC_TGCA'GG
Cuts at: 3071 3071
Size: 5719
ScaI AGT'ACT
Cuts at: 2063 3967 5679 2063
Size: 1904 1712 2103
Fragments arranged by size:
2103 1904 1712
SfcI C'TryA_G
Cuts at: 826 1045 2279 2429 3067 4202 4883 5074 826
Size: 219 1234 150 638 1135 681 191 1471
Fragments arranged by size:
1471 1234 1135 681 638 219 191 150
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5719
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5719
SmaI CCC'GGG
Cuts at: 1872 3046 1872
Size: 1174 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2575 2861 3823 4724 4986 820
Size: 197 926 632 286 962 901 262 1553
Fragments arranged by size:
1553 962 926 901 632 286 262 197
SnaBI TAC'GTA
Cuts at: 493 3510 493
Size: 3017 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5719
SphI G_CATG'C
Cuts at: 1132 3077 1132
Size: 1945 3774
SspI AAT'ATT
Cuts at: 5 52 3643 5
Size: 47 3591 2081
Fragments arranged by size:
3591 2081 47
StuI AGG'CCT
Cuts at: 1517 2258 1517
Size: 741 4978
StyI C'CwwG_G
Cuts at: 513 1065 3158 513
Size: 552 2093 3074
Fragments arranged by size:
3074 2093 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3746 3763 3916 4101 4546 325
Size: 1255 2166 17 153 185 445 1498
Fragments arranged by size:
2166 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2061 2157 3965
Size: 274 80 33 51 1443 84 96 1808
Cuts at: 3965 5677 96
Size: 1712 138
Fragments arranged by size:
1808 1712 1443 274 138 96 84 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5679 5711 5679
Size: 32 5687
Tth111I GACn'n_nGTC
Cuts at: 1316 2392 1316
Size: 1076 4643
XbaI T'CTAG_A
Cuts at: 2293 2308 3055 2293
Size: 15 747 4957
Fragments arranged by size:
4957 747 15
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5719
XmaI C'CCGG_G
Cuts at: 1870 3044 1870
Size: 1174 4545
XmnI GAAnn'nnTTC
Cuts at: 3848 3848
Size: 5719
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3527 276
Size: 53 83 186 936 1993 2468
Fragments arranged by size:
2468 1993 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI
EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PmlI PpuMI PspXI PsrI RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI