Restriction map for
pFN21AE1403
(Circular) (Six-base) MAPSORT of: pFN21AE1403.seq Check: 4917 from: 1 to: 5827
pFN21AE1403 5827 bp
With 182 enzymes: SgfI *
August 16, 2010 14:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2253 3637 278
Size: 53 83 186 936 717 1384 2468
Fragments arranged by size:
2468 1384 936 717 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3170 1937
Size: 1233 4594
Acc65I G'GTAC_C
Cuts at: 3148 3148
Size: 5827
AclI AA'CG_TT
Cuts at: 1563 3954 4327 1563
Size: 2391 373 3063
Fragments arranged by size:
3063 2391 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3008 3890 5274 5542 1632
Size: 1376 882 1384 268 1917
Fragments arranged by size:
1917 1384 1376 882 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5630
AflIII A'CryG_T
Cuts at: 1203 2706 4726 5703 1203
Size: 1503 2020 977 1327
Fragments arranged by size:
2020 1503 1327 977
AgeI A'CCGG_T
Cuts at: 2327 2430 4658 2327
Size: 103 2228 3496
Fragments arranged by size:
3496 2228 103
AhdI GACnn_n'nnGTC
Cuts at: 3109 4556 3109
Size: 1447 4380
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5827
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5827
AlwNI CAG_nnn'CTG
Cuts at: 3570 5142 3570
Size: 1572 4255
ApaLI G'TGCA_C
Cuts at: 3884 5040 3884
Size: 1156 4671
ApoI r'AATT_y
Cuts at: 1493 2023 2740 2842 3136 3362 3514 5623 1493
Size: 530 717 102 294 226 152 2109 1697
Fragments arranged by size:
2109 1697 717 530 294 226 152 102
AseI AT'TA_AT
Cuts at: 160 1032 4381 160
Size: 872 3349 1606
Fragments arranged by size:
3349 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5827
AvaI C'yCGr_G
Cuts at: 1870 1943 3152 1870
Size: 73 1209 4545
Fragments arranged by size:
4545 1209 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2588 2621 2588
Size: 33 5794
BamHI G'GATC_C
Cuts at: 3157 3524 3157
Size: 367 5460
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3148 4603 618
Size: 325 217 627 1361 1455 1842
Fragments arranged by size:
1842 1455 1361 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2625 3146 1391
Size: 1234 521 4072
Fragments arranged by size:
4072 1234 521
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2548 928
Size: 971 649 4207
Fragments arranged by size:
4207 971 649
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4018 4052 1903
Size: 34 2081 34 3678
Fragments arranged by size:
3678 2081 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3719 4929 3719
Size: 1210 4617
BclI T'GATC_A
Cuts at: 1548 2208 1548
Size: 660 5167
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4438 136
Size: 107 122 71 631 605 2766 1525
Fragments arranged by size:
2766 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2991 5822 2991
Size: 2831 2996
BlpI GC'TnA_GC
Cuts at: 3244 3244
Size: 5827
Bme1580I G_kGCm'C
Cuts at: 1790 3888 5044 1790
Size: 2098 1156 2573
Fragments arranged by size:
2573 2098 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5827
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3558 4516 442
Size: 416 53 83 399 24 2141 958 1753
Fragments arranged by size:
2141 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5827
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2029 2122 2524 4487 1239
Size: 790 93 402 1963 2579
Fragments arranged by size:
2579 1963 790 402 93
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2675 3952 4817 5115 2675
Size: 1277 865 298 3387
Fragments arranged by size:
3387 1277 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3083 4490 882
Size: 617 1584 1407 2219
Fragments arranged by size:
2219 1584 1407 617
BsaAI yAC'GTr
Cuts at: 493 1204 2355 3618 493
Size: 711 1151 1263 2702
Fragments arranged by size:
2702 1263 1151 711
BsaBI GATnn'nnATC
Cuts at: 5821 5821
Size: 5827
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2250 3634 4016 4651
Size: 53 83 186 936 717 1384 382 635
Cuts at: 4651 275
Size: 1451
Fragments arranged by size:
1451 1384 936 717 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2327 2430 4260 4658 4932 5079 5490 2327
Size: 103 1830 398 274 147 411 2664
Fragments arranged by size:
2664 1830 411 398 274 147 103
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5602
Fragments arranged by size:
5602 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5827
BseYI C'CCAG_C
Cuts at: 5030 5526 5030
Size: 496 5331
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2909 1163
Size: 573 1173 4081
Fragments arranged by size:
4081 1173 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3277 4038 5066 1540
Size: 275 186 1276 761 1028 2301
Fragments arranged by size:
2301 1276 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2114 2949 3888 3973 5044 2114
Size: 835 939 85 1071 2897
Fragments arranged by size:
2897 1071 939 835 85
BsmI GAATG_Cn'
Cuts at: 3338 3431 3338
Size: 93 5734
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2114 2625 2949 3146 3888 3973 5044
Size: 399 324 511 324 197 742 85 1071
Cuts at: 5044 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 511 399 324 324 197
85
BspEI T'CCGG_A
Cuts at: 5490 5490
Size: 5827
BspHI T'CATG_A
Cuts at: 2691 3716 5446 2691
Size: 1025 1730 3072
Fragments arranged by size:
3072 1730 1025
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3182 844
Size: 2338 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2010 3714 1394
Size: 429 187 1704 3507
Fragments arranged by size:
3507 1704 429 187
BsrDI GCAATG_nn'
Cuts at: 65 1248 2271 2353 4322 4496 65
Size: 1183 1023 82 1969 174 1396
Fragments arranged by size:
1969 1396 1183 1023 174 82
BsrFI r'CCGG_y
Cuts at: 1700 1810 2327 2430 4471 4658 1700
Size: 110 517 103 2041 187 2869
Fragments arranged by size:
2869 2041 517 187 110 103
BsrGI T'GTAC_A
Cuts at: 96 2446 96
Size: 2350 3477
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5827
BssSI C'ACGA_G
Cuts at: 3887 4899 3887
Size: 1012 4815
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5827
BstBI TT'CG_AA
Cuts at: 4631 4631
Size: 5827
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5827
BstYI r'GATC_y
Cuts at: 1972 2991 3157 3524 3909 3926 5378 5464 5476
Size: 1019 166 367 385 17 1452 86 12
Cuts at: 5476 5822 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1019 385 367 346 166 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3034 3034
Size: 5827
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5275
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2046 2422 2601 487
Size: 683 56 820 376 179 3713
Fragments arranged by size:
3713 820 683 376 179 56
BtsI GCAGTG_nn'
Cuts at: 2319 2926 3343 4137 4157 2319
Size: 607 417 794 20 3989
Fragments arranged by size:
3989 794 607 417 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5827
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5792
DraI TTT'AAA
Cuts at: 3131 3131
Size: 5827
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4834 809
Size: 507 3518 1802
Fragments arranged by size:
3518 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2268 3274 4163
Size: 54 1425 50 275 18 438 1006 889
Cuts at: 4163 5792 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1006 889 438 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3274 1537
Size: 275 1462 4090
Fragments arranged by size:
4090 1462 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3757 1353
Size: 2404 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4414 4788 4934 387
Size: 1064 138 2825 374 146 1280
Fragments arranged by size:
2825 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2029 2122 2524 3008 3890 4487 5274
Size: 393 397 93 402 484 882 597 787
Cuts at: 5274 5542 1239
Size: 268 1524
Fragments arranged by size:
1524 882 787 597 484 402 397 393
268 93
EcoO109I rG'GnC_Cy
Cuts at: 3001 3001
Size: 5827
EcoRI G'AATT_C
Cuts at: 2740 3136 3514 2740
Size: 396 378 5053
Fragments arranged by size:
5053 396 378
FspI TGC'GCA
Cuts at: 4333 4333
Size: 5827
HaeII r_GCGC'y
Cuts at: 1678 4974 1678
Size: 3296 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3095 3127 4468 4500 4542
Size: 32 135 32 1523 32 1341 32 42
Cuts at: 4542 4574 1373
Size: 32 2626
Fragments arranged by size:
2626 1523 1341 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3171 3417 669
Size: 988 281 1233 246 3079
Fragments arranged by size:
3079 1233 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5827
HpaI GTT'AAC
Cuts at: 3417 3417
Size: 5827
KpnI G_GTAC'C
Cuts at: 3152 3152
Size: 5827
MfeI C'AATT_G
Cuts at: 3426 3426
Size: 5827
MluI A'CGCG_T
Cuts at: 5703 5703
Size: 5827
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4941 5125 1305
Size: 3636 184 2007
Fragments arranged by size:
3636 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2270 10
Size: 54 1425 343 438 3567
Fragments arranged by size:
3567 1425 438 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2423 2777 3785 4144 4303 518
Size: 648 1257 354 1008 359 159 2042
Fragments arranged by size:
2042 1257 1008 648 359 354 159
MspA1I CmG'CkG
Cuts at: 1606 3243 3920 5068 5313 1606
Size: 1637 677 1148 245 2120
Fragments arranged by size:
2120 1637 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5275
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5827
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5827
NotI GC'GGCC_GC
Cuts at: 3274 3274
Size: 5827
NspI r_CATG'y
Cuts at: 1132 1229 3185 4730 1132
Size: 97 1956 1545 2229
Fragments arranged by size:
2229 1956 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5827
PciI A'CATG_T
Cuts at: 4726 4726
Size: 5827
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2631 1108
Size: 1523 4304
PmeI GTTT'AAAC
Cuts at: 3131 3131
Size: 5827
PmlI CAC'GTG
Cuts at: 2355 2355
Size: 5827
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3896 3928 5409 5441 3896
Size: 32 1481 32 4282
Fragments arranged by size:
4282 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3001 3001
Size: 5827
PshAI GACnn'nnGTC
Cuts at: 1613 2249 1613
Size: 636 5191
PsiI TTA'TAA
Cuts at: 2226 3397 2226
Size: 1171 4656
PstI C_TGCA'G
Cuts at: 830 3179 830
Size: 2349 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5827
SalI G'TCGA_C
Cuts at: 1936 3169 1936
Size: 1233 4594
SbfI CC_TGCA'GG
Cuts at: 3179 3179
Size: 5827
ScaI AGT'ACT
Cuts at: 4075 5787 4075
Size: 1712 4115
SfcI C'TryA_G
Cuts at: 826 1045 3175 4310 4991 5182 826
Size: 219 2130 1135 681 191 1471
Fragments arranged by size:
2130 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5827
SgrAI Cr'CCGG_yG
Cuts at: 1700 2327 1700
Size: 627 5200
SmaI CCC'GGG
Cuts at: 1872 3154 1872
Size: 1282 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2654 3931 4832 5094 820
Size: 197 926 711 1277 901 262 1553
Fragments arranged by size:
1553 1277 926 901 711 262 197
SnaBI TAC'GTA
Cuts at: 493 3618 493
Size: 3125 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5827
SphI G_CATG'C
Cuts at: 1132 3185 1132
Size: 2053 3774
SspI AAT'ATT
Cuts at: 5 52 2197 3751 5
Size: 47 2145 1554 2081
Fragments arranged by size:
2145 2081 1554 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5827
StyI C'CwwG_G
Cuts at: 513 1065 2578 2724 3266 513
Size: 552 1513 146 542 3074
Fragments arranged by size:
3074 1513 552 542 146
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2591 3854 3871 4024 4209 4654 325
Size: 1255 1011 1263 17 153 185 445 1498
Fragments arranged by size:
1498 1263 1255 1011 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2446 3024 4073
Size: 274 80 33 51 1443 469 578 1049
Cuts at: 4073 5785 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1049 578 469 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2993 3025 5787 5819 2993
Size: 32 2762 32 3001
Fragments arranged by size:
3001 2762 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2458 1316
Size: 1142 4685
XbaI T'CTAG_A
Cuts at: 3163 3163
Size: 5827
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5827
XmaI C'CCGG_G
Cuts at: 1870 3152 1870
Size: 1282 4545
XmnI GAAnn'nnTTC
Cuts at: 3956 3956
Size: 5827
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2251 3635 276
Size: 53 83 186 936 717 1384 2468
Fragments arranged by size:
2468 1384 936 717 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI