Restriction map for
pFN21AE1402
(Circular) (Six-base) MAPSORT of: pFN21AE1402.seq Check: 5752 from: 1 to: 6502
pFN21AE1402 6502 bp
With 182 enzymes: SgfI *
August 16, 2010 14:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4312 278
Size: 53 83 186 936 2776 2468
Fragments arranged by size:
2776 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2685 3845 1937
Size: 748 1160 4594
Fragments arranged by size:
4594 1160 748
Acc65I G'GTAC_C
Cuts at: 3823 3823
Size: 6502
AclI AA'CG_TT
Cuts at: 1563 4629 5002 1563
Size: 3066 373 3063
Fragments arranged by size:
3066 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2969 3713 4565 5949 6217 1632
Size: 1337 744 852 1384 268 1917
Fragments arranged by size:
1917 1384 1337 852 744 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6305
AflIII A'CryG_T
Cuts at: 1203 5401 6378 1203
Size: 4198 977 1327
Fragments arranged by size:
4198 1327 977
AgeI A'CCGG_T
Cuts at: 5333 5333
Size: 6502
AhdI GACnn_n'nnGTC
Cuts at: 5231 5231
Size: 6502
AleI CACnn'nnGTG
Cuts at: 1166 2096 2931 1166
Size: 930 835 4737
Fragments arranged by size:
4737 930 835
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6502
AlwNI CAG_nnn'CTG
Cuts at: 2530 2770 3055 4245 5817 2530
Size: 240 285 1190 1572 3215
Fragments arranged by size:
3215 1572 1190 285 240
ApaLI G'TGCA_C
Cuts at: 4559 5715 4559
Size: 1156 5346
ApoI r'AATT_y
Cuts at: 1493 2239 3080 3811 4037 4189 6298 1493
Size: 746 841 731 226 152 2109 1697
Fragments arranged by size:
2109 1697 841 746 731 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5056 160
Size: 872 4024 1606
Fragments arranged by size:
4024 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6502
AvaI C'yCGr_G
Cuts at: 1870 1943 2829 3366 3827 1870
Size: 73 886 537 461 4545
Fragments arranged by size:
4545 886 537 461 73
AvrII C'CTAG_G
Cuts at: 3604 3604
Size: 6502
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2744 2777 2744
Size: 33 6469
BamHI G'GATC_C
Cuts at: 3832 4199 3832
Size: 367 6135
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2086 2579 2806 3162 3823
Size: 325 217 627 299 493 227 356 661
Cuts at: 3823 5278 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 661 627 493 356 325 299
227 217
BanII G_rGCy'C
Cuts at: 1391 2895 3197 3444 3583 3801 3821 1391
Size: 1504 302 247 139 218 20 4072
Fragments arranged by size:
4072 1504 302 247 218 139 20
BbeI G_GCGC'C
Cuts at: 2090 2810 2090
Size: 720 5782
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5531
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4693 4727 1903
Size: 34 2756 34 3678
Fragments arranged by size:
3678 2756 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4394 5604 4394
Size: 1210 5292
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6502
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5113 136
Size: 107 122 71 631 605 3441 1525
Fragments arranged by size:
3441 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6497 6497
Size: 6502
BlpI GC'TnA_GC
Cuts at: 2955 3919 2955
Size: 964 5538
Bme1580I G_kGCm'C
Cuts at: 1790 4563 5719 1790
Size: 2773 1156 2573
Fragments arranged by size:
2773 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6502
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2370 4233 5191
Size: 416 53 83 399 24 953 1863 958
Cuts at: 5191 442
Size: 1753
Fragments arranged by size:
1863 1753 958 953 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6502
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2351 2443 2351
Size: 92 6410
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2735 5162 1239
Size: 1496 2427 2579
Fragments arranged by size:
2579 2427 1496
Bpu10I CC'TnA_GC
Cuts at: 2549 2549
Size: 6502
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2741 3578 4627 5492 5790 2741
Size: 837 1049 865 298 3453
Fragments arranged by size:
3453 1049 865 837 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3011 3605 5165 882
Size: 617 1512 594 1560 2219
Fragments arranged by size:
2219 1560 1512 617 594
BsaAI yAC'GTr
Cuts at: 493 1204 2921 3046 4293 493
Size: 711 1717 125 1247 2702
Fragments arranged by size:
2702 1717 1247 711 125
BsaBI GATnn'nnATC
Cuts at: 3032 6496 3032
Size: 3464 3038
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2087 2807 4309 4691
Size: 53 83 186 936 554 720 1502 382
Cuts at: 4691 5326 275
Size: 635 1451
Fragments arranged by size:
1502 1451 936 720 635 554 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2025 2235 3625 4935 5333 5607 5754 6165 2025
Size: 210 1390 1310 398 274 147 411 2362
Fragments arranged by size:
2362 1390 1310 411 398 274 210 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2708 2738 3467 3497 1178
Size: 30 165 30 1305 30 729 30 4183
Fragments arranged by size:
4183 1305 729 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3053 1186
Size: 1867 4635
BseYI C'CCAG_C
Cuts at: 2252 2415 2523 5705 6201 2252
Size: 163 108 3182 496 2553
Fragments arranged by size:
3182 2553 496 163 108
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2161 2191 3068 1163
Size: 573 425 30 877 4597
Fragments arranged by size:
4597 877 573 425 30
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3952 4713 5741 1540
Size: 275 186 1951 761 1028 2301
Fragments arranged by size:
2301 1951 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2928 2961 3444 3647 3801 4563 4648 5719 2928
Size: 33 483 203 154 762 85 1071 3711
Fragments arranged by size:
3711 1071 762 483 203 154 85 33
BsmI GAATG_Cn'
Cuts at: 4013 4106 4013
Size: 93 6409
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2895 2928 2961 3197 3444 3583 3647
Size: 399 1105 33 33 236 247 139 64
Cuts at: 3647 3801 3821 4563 4648 5719 1391
Size: 154 20 742 85 1071 2174
Fragments arranged by size:
2174 1105 1071 742 399 247 236 154
139 85 64 33 33 20
BspEI T'CCGG_A
Cuts at: 2235 3625 6165 2235
Size: 1390 2540 2572
Fragments arranged by size:
2572 2540 1390
BspHI T'CATG_A
Cuts at: 4391 6121 4391
Size: 1730 4772
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2952 3857 844
Size: 2108 905 3489
Fragments arranged by size:
3489 2108 905
BsrBI CCG'CTC
Cuts at: 1394 1823 2059 4389 1394
Size: 429 236 2330 3507
Fragments arranged by size:
3507 2330 429 236
BsrDI GCAATG_nn'
Cuts at: 65 1248 3129 4997 5171 65
Size: 1183 1881 1868 174 1396
Fragments arranged by size:
1881 1868 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5146 5333 1700
Size: 110 3336 187 2869
Fragments arranged by size:
3336 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6502
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6502
BssSI C'ACGA_G
Cuts at: 4562 5574 4562
Size: 1012 5490
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6502
BstBI TT'CG_AA
Cuts at: 5306 5306
Size: 6502
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6502
BstXI CCAn_nnnn'nTGG
Cuts at: 2488 2671 2702 3244 3293 2488
Size: 183 31 542 49 5697
Fragments arranged by size:
5697 542 183 49 31
BstYI r'GATC_y
Cuts at: 1972 3832 4199 4584 4601 6053 6139 6151 6497
Size: 1860 367 385 17 1452 86 12 346
Cuts at: 6497 1972
Size: 1977
Fragments arranged by size:
1977 1860 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2487 2677 3135 513
Size: 552 1422 190 458 3880
Fragments arranged by size:
3880 1422 552 458 190
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2988 487
Size: 683 56 1762 4001
Fragments arranged by size:
4001 1762 683 56
BtsI GCAGTG_nn'
Cuts at: 2243 4018 4812 4832 2243
Size: 1775 794 20 3913
Fragments arranged by size:
3913 1775 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6502
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6467
DraI TTT'AAA
Cuts at: 3806 3806
Size: 6502
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5509 809
Size: 507 4193 1802
Fragments arranged by size:
4193 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2692 3295 3684
Size: 54 1425 50 275 18 862 603 389
Cuts at: 3684 3949 4838 6467 8
Size: 265 889 1629 43
Fragments arranged by size:
1629 1425 889 862 603 389 275 265
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3949 1537
Size: 275 2137 4090
Fragments arranged by size:
4090 2137 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2796 4432 1353
Size: 1443 1636 3423
Fragments arranged by size:
3423 1636 1443
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5089 5463 5609 387
Size: 1064 138 3500 374 146 1280
Fragments arranged by size:
3500 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3442 3799 3442
Size: 357 6145
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2735 2969 3713 4565 5162 5949 6217
Size: 393 1103 234 744 852 597 787 268
Cuts at: 6217 1239
Size: 1524
Fragments arranged by size:
1524 1103 852 787 744 597 393 268
234
EcoNI CCTnn'n_nnAGG
Cuts at: 2948 2948
Size: 6502
EcoO109I rG'GnC_Cy
Cuts at: 2651 3262 2651
Size: 611 5891
EcoRI G'AATT_C
Cuts at: 2239 3811 4189 2239
Size: 1572 378 4552
Fragments arranged by size:
4552 1572 378
FspI TGC'GCA
Cuts at: 5008 5008
Size: 6502
HaeII r_GCGC'y
Cuts at: 1678 2090 2293 2810 5649 1678
Size: 412 203 517 2839 2531
Fragments arranged by size:
2839 2531 517 412 203
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 1999 2031 3263 3295 3565
Size: 32 135 32 427 32 1232 32 270
Cuts at: 3565 3597 3773 3805 5143 5175 5217 5249 1373
Size: 32 176 32 1338 32 42 32 2626
Fragments arranged by size:
2626 1338 1232 427 270 176 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3846 4092 669
Size: 988 281 1908 246 3079
Fragments arranged by size:
3079 1908 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6502
HpaI GTT'AAC
Cuts at: 4092 4092
Size: 6502
KasI G'GCGC_C
Cuts at: 2086 2806 2086
Size: 720 5782
KpnI G_GTAC'C
Cuts at: 3827 3827
Size: 6502
MfeI C'AATT_G
Cuts at: 4101 4101
Size: 6502
MluI A'CGCG_T
Cuts at: 6378 6378
Size: 6502
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2388 2454 3453 3483 5616 5800 1305
Size: 1083 66 999 30 2133 184 2007
Fragments arranged by size:
2133 2007 1083 999 184 66 30
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4680
Fragments arranged by size:
4680 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2096 2486 2700 2931 3134 3236 3242
Size: 648 930 390 214 231 203 102 6
Cuts at: 3242 4460 4819 4978 518
Size: 1218 359 159 2042
Fragments arranged by size:
2042 1218 930 648 390 359 231 214
203 159 102 6
MspA1I CmG'CkG
Cuts at: 1606 2415 3055 3076 3918 4595 5743 5988 1606
Size: 809 640 21 842 677 1148 245 2120
Fragments arranged by size:
2120 1148 842 809 677 640 245 21
NarI GG'CG_CC
Cuts at: 2087 2807 2087
Size: 720 5782
NcoI C'CATG_G
Cuts at: 513 1065 2487 2677 3135 513
Size: 552 1422 190 458 3880
Fragments arranged by size:
3880 1422 552 458 190
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6502
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6502
NotI GC'GGCC_GC
Cuts at: 3949 3949
Size: 6502
NspI r_CATG'y
Cuts at: 1132 1229 3341 3860 5405 1132
Size: 97 2112 519 1545 2229
Fragments arranged by size:
2229 2112 1545 519 97
PasI CC'CwG_GG
Cuts at: 1348 2081 1348
Size: 733 5769
PciI A'CATG_T
Cuts at: 5401 5401
Size: 6502
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2530 2770 2913 1108
Size: 1422 240 143 4697
Fragments arranged by size:
4697 1422 240 143
PfoI T'CCnGG_A
Cuts at: 3435 3435
Size: 6502
PmeI GTTT'AAAC
Cuts at: 3806 3806
Size: 6502
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3083 3115 4571 4603 6084 6116 3083
Size: 32 1456 32 1481 32 3469
Fragments arranged by size:
3469 1481 1456 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2651 2651
Size: 6502
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6502
PsiI TTA'TAA
Cuts at: 4072 4072
Size: 6502
PstI C_TGCA'G
Cuts at: 830 3075 3384 3854 830
Size: 2245 309 470 3478
Fragments arranged by size:
3478 2245 470 309
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6502
PvuII CAG'CTG
Cuts at: 2415 3055 3076 2415
Size: 640 21 5841
Fragments arranged by size:
5841 640 21
SacI G_AGCT'C
Cuts at: 3444 3801 3444
Size: 357 6145
SalI G'TCGA_C
Cuts at: 1936 3844 1936
Size: 1908 4594
SbfI CC_TGCA'GG
Cuts at: 3854 3854
Size: 6502
ScaI AGT'ACT
Cuts at: 3632 4750 6462 3632
Size: 1118 1712 3672
Fragments arranged by size:
3672 1712 1118
SexAI A'CCwGG_T
Cuts at: 2763 2763
Size: 6502
SfcI C'TryA_G
Cuts at: 826 1045 3071 3380 3850 4985 5666 5857 826
Size: 219 2026 309 470 1135 681 191 1471
Fragments arranged by size:
2026 1471 1135 681 470 309 219 191
SfoI GGC'GCC
Cuts at: 2088 2808 2088
Size: 720 5782
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6502
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6502
SmaI CCC'GGG
Cuts at: 1872 3829 1872
Size: 1957 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2756 3593 4606 5507 5769 820
Size: 197 926 813 837 1013 901 262 1553
Fragments arranged by size:
1553 1013 926 901 837 813 262 197
SnaBI TAC'GTA
Cuts at: 493 4293 493
Size: 3800 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6502
SphI G_CATG'C
Cuts at: 1132 3860 1132
Size: 2728 3774
SspI AAT'ATT
Cuts at: 5 52 4426 5
Size: 47 4374 2081
Fragments arranged by size:
4374 2081 47
StuI AGG'CCT
Cuts at: 1517 2669 1517
Size: 1152 5350
StyI C'CwwG_G
Cuts at: 513 1065 2487 2575 2664 2670 2677 3135 3604
Size: 552 1422 88 89 6 7 458 469
Cuts at: 3604 3941 513
Size: 337 3074
Fragments arranged by size:
3074 1422 552 469 458 337 89 88
7 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2282 2500 2633 4529 4546 4699 4884
Size: 1255 702 218 133 1896 17 153 185
Cuts at: 4884 5329 325
Size: 445 1498
Fragments arranged by size:
1896 1498 1255 702 445 218 185 153
133 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3204 3630 4748
Size: 274 80 33 51 1443 1227 426 1118
Cuts at: 4748 6460 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1227 1118 426 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6462 6494 6462
Size: 32 6470
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6502
XbaI T'CTAG_A
Cuts at: 3838 3838
Size: 6502
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2771 2873 2771
Size: 102 6400
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6502
XmaI C'CCGG_G
Cuts at: 1870 3827 1870
Size: 1957 4545
XmnI GAAnn'nnTTC
Cuts at: 4631 4631
Size: 6502
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4310 276
Size: 53 83 186 936 2776 2468
Fragments arranged by size:
2776 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacI SalI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BbvCI BfrBI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI
NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI
PsrI RsrII SacII SanDI SapI SfiI SrfI SwaI