Restriction map for
pFN21AE1398
(Circular) (Six-base) MAPSORT of: pFN21AE1398.seq Check: 4694 from: 1 to: 8011
pFN21AE1398 8011 bp
With 182 enzymes: SgfI *
June 19, 2019 18:49 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5821 278
Size: 53 83 186 936 4285 2468
Fragments arranged by size:
4285 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3015 3555 5354 1937
Size: 1078 540 1799 4594
Fragments arranged by size:
4594 1799 1078 540
Acc65I G'GTAC_C
Cuts at: 5332 5332
Size: 8011
AclI AA'CG_TT
Cuts at: 1563 5009 5310 6138 6511 1563
Size: 3446 301 828 373 3063
Fragments arranged by size:
3446 3063 828 373 301
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2221 2438 6074 7458 7726 1632
Size: 589 217 3636 1384 268 1917
Fragments arranged by size:
3636 1917 1384 589 268 217
AfeI AGC'GCT
Cuts at: 2234 2234
Size: 8011
AflII C'TTAA_G
Cuts at: 820 1017 4646 820
Size: 197 3629 4185
Fragments arranged by size:
4185 3629 197
AflIII A'CryG_T
Cuts at: 1203 3546 3750 4254 6910 7887 1203
Size: 2343 204 504 2656 977 1327
Fragments arranged by size:
2656 2343 1327 977 504 204
AgeI A'CCGG_T
Cuts at: 6842 6842
Size: 8011
AhdI GACnn_n'nnGTC
Cuts at: 3022 3457 3517 6740 3022
Size: 435 60 3223 4293
Fragments arranged by size:
4293 3223 435 60
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8011
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8011
AlwNI CAG_nnn'CTG
Cuts at: 5083 5754 7326 5083
Size: 671 1572 5768
Fragments arranged by size:
5768 1572 671
ApaLI G'TGCA_C
Cuts at: 6068 7224 6068
Size: 1156 6855
ApoI r'AATT_y
Cuts at: 1493 3185 3281 3308 3440 3861 4225 4239 5120
Size: 1692 96 27 132 421 364 14 881
Cuts at: 5120 5179 5320 5546 5698 7807 1493
Size: 59 141 226 152 2109 1697
Fragments arranged by size:
2109 1697 1692 881 421 364 226 152
141 132 96 59 27 14
AseI AT'TA_AT
Cuts at: 160 1032 6565 160
Size: 872 5533 1606
Fragments arranged by size:
5533 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8011
AvaI C'yCGr_G
Cuts at: 1870 1943 2063 4004 5336 1870
Size: 73 120 1941 1332 4545
Fragments arranged by size:
4545 1941 1332 120 73
BamHI G'GATC_C
Cuts at: 5341 5708 5341
Size: 367 7644
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2942 2957 5332 6787 618
Size: 325 217 627 1155 15 2375 1455 1842
Fragments arranged by size:
2375 1842 1455 1155 627 325 217 15
BanII G_rGCy'C
Cuts at: 1391 2064 3957 5241 5330 1391
Size: 673 1893 1284 89 4072
Fragments arranged by size:
4072 1893 1284 673 89
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4993 5169 928
Size: 971 3094 176 3770
Fragments arranged by size:
3770 3094 971 176
BbvCI CC'TCA_GC
Cuts at: 3615 5030 3615
Size: 1415 6596
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3995 4029 6202 6236 1903
Size: 34 2058 34 2173 34 3678
Fragments arranged by size:
3678 2173 2058 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5028 5903 7113 5028
Size: 875 1210 5926
Fragments arranged by size:
5926 1210 875
BclI T'GATC_A
Cuts at: 1548 2127 1548
Size: 579 7432
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6622 136
Size: 107 122 71 631 605 4950 1525
Fragments arranged by size:
4950 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4369 4591 8006 4369
Size: 222 3415 4374
Fragments arranged by size:
4374 3415 222
BlpI GC'TnA_GC
Cuts at: 5428 5428
Size: 8011
Bme1580I G_kGCm'C
Cuts at: 1790 2960 6072 7228 1790
Size: 1170 3112 1156 2573
Fragments arranged by size:
3112 2573 1170 1156
BmgBI CAC'GTC
Cuts at: 1318 2388 3454 4255 1318
Size: 1070 1066 801 5074
Fragments arranged by size:
5074 1070 1066 801
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4151 5742 6700
Size: 416 53 83 399 24 2734 1591 958
Cuts at: 6700 442
Size: 1753
Fragments arranged by size:
2734 1753 1591 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8011
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2930 3849 2930
Size: 919 7092
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2345 2542 6671 1239
Size: 1106 197 4129 2579
Fragments arranged by size:
4129 2579 1106 197
Bpu10I CC'TnA_GC
Cuts at: 2261 3615 5030 2261
Size: 1354 1415 5242
Fragments arranged by size:
5242 1415 1354
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4515 6136 7001 7299 4515
Size: 1621 865 298 5227
Fragments arranged by size:
5227 1621 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6674 882
Size: 617 5175 2219
Fragments arranged by size:
5175 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4145 5802 493
Size: 711 2941 1657 2702
Fragments arranged by size:
2941 2702 1657 711
BsaBI GATnn'nnATC
Cuts at: 4047 4655 8005 4047
Size: 608 3350 4053
Fragments arranged by size:
4053 3350 608
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5818 6200 6835 275
Size: 53 83 186 936 4285 382 635 1451
Fragments arranged by size:
4285 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 6444 6842 7116 7263 7674 6444
Size: 398 274 147 411 6781
Fragments arranged by size:
6781 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 5090 5120 1178
Size: 30 165 30 3687 30 4069
Fragments arranged by size:
4069 3687 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2186 3793 5281 1186
Size: 1000 1607 1488 3916
Fragments arranged by size:
3916 1607 1488 1000
BseYI C'CCAG_C
Cuts at: 3146 7214 7710 3146
Size: 4068 496 3447
Fragments arranged by size:
4068 3447 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3517 3725 1163
Size: 573 1781 208 5449
Fragments arranged by size:
5449 1781 573 208
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5461 6222 7250 1540
Size: 275 186 3460 761 1028 2301
Fragments arranged by size:
3460 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3492 3957 6072 6157 7228 3492
Size: 465 2115 85 1071 4275
Fragments arranged by size:
4275 2115 1071 465 85
BsmI GAATG_Cn'
Cuts at: 5522 5615 5522
Size: 93 7918
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2064 2960 3492 3957 5241 5330 6072
Size: 399 274 896 532 465 1284 89 742
Cuts at: 6072 6157 7228 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1284 1071 896 742 532 465 399
274 89 85
BspEI T'CCGG_A
Cuts at: 7674 7674
Size: 8011
BspHI T'CATG_A
Cuts at: 4051 5900 7630 4051
Size: 1849 1730 4432
Fragments arranged by size:
4432 1849 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5366 844
Size: 4522 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3676 5898 1394
Size: 429 1853 2222 3507
Fragments arranged by size:
3507 2222 1853 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2374 3194 3918 6506 6680 65
Size: 1183 1126 820 724 2588 174 1396
Fragments arranged by size:
2588 1396 1183 1126 820 724 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6655 6842 1700
Size: 110 4845 187 2869
Fragments arranged by size:
4845 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2587 2730 96
Size: 2491 143 5377
Fragments arranged by size:
5377 2491 143
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8011
BssSI C'ACGA_G
Cuts at: 6071 7083 6071
Size: 1012 6999
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8011
BstBI TT'CG_AA
Cuts at: 4915 6815 4915
Size: 1900 6111
BstEII G'GTnAC_C
Cuts at: 1184 3595 1184
Size: 2411 5600
BstXI CCAn_nnnn'nTGG
Cuts at: 2515 3731 2515
Size: 1216 6795
BstYI r'GATC_y
Cuts at: 1972 3325 4369 4501 4591 5341 5708 6093 6110
Size: 1353 1044 132 90 750 367 385 17
Cuts at: 6110 7562 7648 7660 8006 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1353 1044 750 385 367 346
132 90 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3330 3330
Size: 8011
BtgI C'CryG_G
Cuts at: 513 1065 2166 2291 2605 2977 3840 4788 513
Size: 552 1101 125 314 372 863 948 3736
Fragments arranged by size:
3736 1101 948 863 552 372 314 125
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7272
Fragments arranged by size:
7272 683 56
BtsI GCAGTG_nn'
Cuts at: 2418 3529 5527 6321 6341 2418
Size: 1111 1998 794 20 4088
Fragments arranged by size:
4088 1998 1111 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8011
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7976
DraI TTT'AAA
Cuts at: 4687 5119 5315 4687
Size: 432 196 7383
Fragments arranged by size:
7383 432 196
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7018 809
Size: 507 5702 1802
Fragments arranged by size:
5702 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3733 5458 6347
Size: 54 1425 50 275 18 1903 1725 889
Cuts at: 6347 7976 8
Size: 1629 43
Fragments arranged by size:
1903 1725 1629 1425 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5458 1537
Size: 275 3646 4090
Fragments arranged by size:
4090 3646 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3028 3636 4713 5941 1353
Size: 1675 608 1077 1228 3423
Fragments arranged by size:
3423 1675 1228 1077 608
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2609 6598 6972 7118 387
Size: 1064 138 1020 3989 374 146 1280
Fragments arranged by size:
3989 1280 1064 1020 374 146 138
EcoICRI GAG'CTC
Cuts at: 3955 3955
Size: 8011
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2221 2345 2438 2542 6074 6671 7458
Size: 393 589 124 93 104 3532 597 787
Cuts at: 7458 7726 1239
Size: 268 1524
Fragments arranged by size:
3532 1524 787 597 589 393 268 124
104 93
EcoNI CCTnn'n_nnAGG
Cuts at: 2544 4208 5232 5288 2544
Size: 1664 1024 56 5267
Fragments arranged by size:
5267 1664 1024 56
EcoO109I rG'GnC_Cy
Cuts at: 3334 4762 3334
Size: 1428 6583
EcoRI G'AATT_C
Cuts at: 3281 4239 5320 5698 3281
Size: 958 1081 378 5594
Fragments arranged by size:
5594 1081 958 378
EcoRV GAT'ATC
Cuts at: 3052 3052
Size: 8011
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2790 2790
Size: 8011
FspI TGC'GCA
Cuts at: 6517 6517
Size: 8011
HaeII r_GCGC'y
Cuts at: 1678 2203 2236 7158 1678
Size: 525 33 4922 2531
Fragments arranged by size:
4922 2531 525 33
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2343 2372 2375 2404 2432
Size: 32 135 32 771 29 3 29 28
Cuts at: 2432 2464 2744 2776 3008 3040 3443 3475 3503
Size: 32 280 32 232 32 403 32 28
Cuts at: 3503 3535 3643 3675 4066 4098 4793 4825 6652
Size: 32 108 32 391 32 695 32 1827
Cuts at: 6652 6684 6726 6758 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1827 771 695 403 391 280 232
135 108 42 32 32 32 32 32
32 32 32 32 32 32 32 29
29 28 28 3
HincII GTy'rAC
Cuts at: 669 1657 1938 2841 5355 5601 669
Size: 988 281 903 2514 246 3079
Fragments arranged by size:
3079 2514 988 903 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8011
HpaI GTT'AAC
Cuts at: 2841 5601 2841
Size: 2760 5251
KpnI G_GTAC'C
Cuts at: 5336 5336
Size: 8011
MfeI C'AATT_G
Cuts at: 3483 5127 5610 3483
Size: 1644 483 5884
Fragments arranged by size:
5884 1644 483
MluI A'CGCG_T
Cuts at: 7887 7887
Size: 8011
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 7125 7309 1305
Size: 5820 184 2007
Fragments arranged by size:
5820 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3735 10
Size: 54 1425 343 1903 4286
Fragments arranged by size:
4286 1903 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2075 2854 3495 4572 4752 5969 6328
Size: 648 909 779 641 1077 180 1217 359
Cuts at: 6328 6487 518
Size: 159 2042
Fragments arranged by size:
2042 1217 1077 909 779 648 641 359
180 159
MspA1I CmG'CkG
Cuts at: 1606 2168 2979 5427 6104 7252 7497 1606
Size: 562 811 2448 677 1148 245 2120
Fragments arranged by size:
2448 2120 1148 811 677 562 245
NcoI C'CATG_G
Cuts at: 513 1065 2291 2605 3840 513
Size: 552 1226 314 1235 4684
Fragments arranged by size:
4684 1235 1226 552 314
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8011
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8011
NotI GC'GGCC_GC
Cuts at: 5458 5458
Size: 8011
NspI r_CATG'y
Cuts at: 1132 1229 3550 3754 5369 6914 1132
Size: 97 2321 204 1615 1545 2229
Fragments arranged by size:
2321 2229 1615 1545 204 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8011
PciI A'CATG_T
Cuts at: 3546 3750 6910 3546
Size: 204 3160 4647
Fragments arranged by size:
4647 3160 204
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2391 1108
Size: 1283 6728
PfoI T'CCnGG_A
Cuts at: 3987 4423 4758 5094 3987
Size: 436 335 336 6904
Fragments arranged by size:
6904 436 336 335
PmeI GTTT'AAAC
Cuts at: 5315 5315
Size: 8011
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6080 6112 7593 7625 6080
Size: 32 1481 32 6466
Fragments arranged by size:
6466 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3334 4762 3334
Size: 1428 6583
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8011
PsiI TTA'TAA
Cuts at: 5581 5581
Size: 8011
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3144 3176 3144
Size: 32 7979
PstI C_TGCA'G
Cuts at: 830 4518 5363 830
Size: 3688 845 3478
Fragments arranged by size:
3688 3478 845
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8011
SacI G_AGCT'C
Cuts at: 3957 3957
Size: 8011
SacII CC_GC'GG
Cuts at: 2169 2980 2169
Size: 811 7200
SalI G'TCGA_C
Cuts at: 1936 5353 1936
Size: 3417 4594
SbfI CC_TGCA'GG
Cuts at: 5363 5363
Size: 8011
ScaI AGT'ACT
Cuts at: 6259 7971 6259
Size: 1712 6299
SexAI A'CCwGG_T
Cuts at: 4938 4938
Size: 8011
SfcI C'TryA_G
Cuts at: 826 1045 2690 2823 4514 4598 5359 6494 7175
Size: 219 1645 133 1691 84 761 1135 681
Cuts at: 7175 7366 826
Size: 191 1471
Fragments arranged by size:
1691 1645 1471 1135 761 681 219 191
133 84
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8011
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8011
SmaI CCC'GGG
Cuts at: 1872 5338 1872
Size: 3466 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4530 4646 6115 7016 7278 820
Size: 197 926 2587 116 1469 901 262 1553
Fragments arranged by size:
2587 1553 1469 926 901 262 197 116
SnaBI TAC'GTA
Cuts at: 493 5802 493
Size: 5309 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8011
SphI G_CATG'C
Cuts at: 1132 5369 1132
Size: 4237 3774
SspI AAT'ATT
Cuts at: 5 52 3984 5935 5
Size: 47 3932 1951 2081
Fragments arranged by size:
3932 2081 1951 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8011
StyI C'CwwG_G
Cuts at: 513 1065 2291 2605 3739 3840 3974 5228 5450
Size: 552 1226 314 1134 101 134 1254 222
Cuts at: 5450 513
Size: 3074
Fragments arranged by size:
3074 1254 1226 1134 552 314 222 134
101
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2245 2549 2844 3012 4740 6038 6055
Size: 1255 665 304 295 168 1728 1298 17
Cuts at: 6055 6208 6393 6838 325
Size: 153 185 445 1498
Fragments arranged by size:
1728 1498 1298 1255 665 445 304 295
185 168 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2587 2730 3467
Size: 274 80 33 51 1443 610 143 737
Cuts at: 3467 5151 6257 7969 96
Size: 1684 1106 1712 138
Fragments arranged by size:
1712 1684 1443 1106 737 610 274 143
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3384 3416 7971 8003 3384
Size: 32 4555 32 3392
Fragments arranged by size:
4555 3392 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8011
XbaI T'CTAG_A
Cuts at: 5347 5347
Size: 8011
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8011
XmaI C'CCGG_G
Cuts at: 1870 5336 1870
Size: 3466 4545
XmnI GAAnn'nnTTC
Cuts at: 6140 6140
Size: 8011
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5819 276
Size: 53 83 186 936 4285 2468
Fragments arranged by size:
4285 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI
PvuI SacI SacII SalI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstZ17I DraIII FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI
PvuII RsrII SanDI SapI SfiI SfoI SrfI SwaI
XcmI