Restriction map for
pFN21AE1396
(Circular) (Six-base) MAPSORT of: pFN21AE1396.seq Check: 9766 from: 1 to: 4906
pFN21AE1396 4906 bp
With 182 enzymes: SgfI *
August 16, 2010 14:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2716 278
Size: 53 83 186 936 1180 2468
Fragments arranged by size:
2468 1180 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2249 1937
Size: 312 4594
Acc65I G'GTAC_C
Cuts at: 2227 2227
Size: 4906
AclI AA'CG_TT
Cuts at: 1563 3033 3406 1563
Size: 1470 373 3063
Fragments arranged by size:
3063 1470 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2969 4353 4621 1632
Size: 1337 1384 268 1917
Fragments arranged by size:
1917 1384 1337 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4709
AflIII A'CryG_T
Cuts at: 1203 2174 3805 4782 1203
Size: 971 1631 977 1327
Fragments arranged by size:
1631 1327 977 971
AgeI A'CCGG_T
Cuts at: 3737 3737
Size: 4906
AhdI GACnn_n'nnGTC
Cuts at: 3635 3635
Size: 4906
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 4906
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 4906
AlwNI CAG_nnn'CTG
Cuts at: 2649 4221 2649
Size: 1572 3334
ApaLI G'TGCA_C
Cuts at: 2963 4119 2963
Size: 1156 3750
ApoI r'AATT_y
Cuts at: 1493 2215 2441 2593 4702 1493
Size: 722 226 152 2109 1697
Fragments arranged by size:
2109 1697 722 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3460 160
Size: 872 2428 1606
Fragments arranged by size:
2428 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4906
AvaI C'yCGr_G
Cuts at: 1870 1943 2231 1870
Size: 73 288 4545
Fragments arranged by size:
4545 288 73
BamHI G'GATC_C
Cuts at: 2010 2236 2603 2010
Size: 226 367 4313
Fragments arranged by size:
4313 367 226
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2050 2227 3682 618
Size: 325 217 627 263 177 1455 1842
Fragments arranged by size:
1842 1455 627 325 263 217 177
BanII G_rGCy'C
Cuts at: 1391 2225 1391
Size: 834 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 3935
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2172 2206 3097 3131 1903
Size: 34 235 34 891 34 3678
Fragments arranged by size:
3678 891 235 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2798 4008 2798
Size: 1210 3696
BclI T'GATC_A
Cuts at: 1548 1548
Size: 4906
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3517 136
Size: 107 122 71 631 605 1845 1525
Fragments arranged by size:
1845 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4901 4901
Size: 4906
BlpI GC'TnA_GC
Cuts at: 2132 2323 2132
Size: 191 4715
Bme1580I G_kGCm'C
Cuts at: 1790 2967 4123 1790
Size: 1177 1156 2573
Fragments arranged by size:
2573 1177 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 4906
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2637 3595 442
Size: 416 53 83 399 24 1220 958 1753
Fragments arranged by size:
1753 1220 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 4906
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3566 1239
Size: 2327 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3031 3896 4194 3031
Size: 865 298 3743
Fragments arranged by size:
3743 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3569 882
Size: 617 2070 2219
Fragments arranged by size:
2219 2070 617
BsaAI yAC'GTr
Cuts at: 493 1204 2697 493
Size: 711 1493 2702
Fragments arranged by size:
2702 1493 711
BsaBI GATnn'nnATC
Cuts at: 4900 4900
Size: 4906
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2713 3095 3730 275
Size: 53 83 186 936 1180 382 635 1451
Fragments arranged by size:
1451 1180 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3339 3737 4011 4158 4569 3339
Size: 398 274 147 411 3676
Fragments arranged by size:
3676 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4681
Fragments arranged by size:
4681 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 4906
BseYI C'CCAG_C
Cuts at: 4109 4605 4109
Size: 496 4410
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4333
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2119 2356 3117 4145 1540
Size: 275 186 118 237 761 1028 2301
Fragments arranged by size:
2301 1028 761 275 237 186 118
BsiHKAI G_wGCw'C
Cuts at: 2133 2171 2967 3052 4123 2133
Size: 38 796 85 1071 2916
Fragments arranged by size:
2916 1071 796 85 38
BsmI GAATG_Cn'
Cuts at: 2417 2510 2417
Size: 93 4813
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2133 2171 2225 2967 3052 4123 1391
Size: 399 343 38 54 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 343 85 54 38
BspEI T'CCGG_A
Cuts at: 4569 4569
Size: 4906
BspHI T'CATG_A
Cuts at: 2795 4525 2795
Size: 1730 3176
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2261 844
Size: 1417 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2793 1394
Size: 429 970 3507
Fragments arranged by size:
3507 970 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3401 3575 65
Size: 1183 2153 174 1396
Fragments arranged by size:
2153 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2118 3550 3737 1700
Size: 110 308 1432 187 2869
Fragments arranged by size:
2869 1432 308 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 4906
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 4906
BssSI C'ACGA_G
Cuts at: 2966 3978 2966
Size: 1012 3894
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 4906
BstBI TT'CG_AA
Cuts at: 3710 3710
Size: 4906
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 4906
BstYI r'GATC_y
Cuts at: 1972 2010 2236 2603 2988 3005 4457 4543 4555
Size: 38 226 367 385 17 1452 86 12
Cuts at: 4555 4901 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 385 367 346 226 86 38
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4354
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4167
Fragments arranged by size:
4167 683 56
BtsI GCAGTG_nn'
Cuts at: 2422 3216 3236 2422
Size: 794 20 4092
Fragments arranged by size:
4092 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 4906
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 4871
DraI TTT'AAA
Cuts at: 2210 2210
Size: 4906
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3913 809
Size: 507 2597 1802
Fragments arranged by size:
2597 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2116 2179 2353
Size: 54 1425 50 275 18 286 63 174
Cuts at: 2353 3242 4871 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 286 275 174 63 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2116 2353 1537
Size: 275 304 237 4090
Fragments arranged by size:
4090 304 275 237
EarI CTCTTCn'nnn_
Cuts at: 1353 2042 2836 1353
Size: 689 794 3423
Fragments arranged by size:
3423 794 689
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3493 3867 4013 387
Size: 1064 138 1904 374 146 1280
Fragments arranged by size:
1904 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2969 3566 4353 4621 1239
Size: 393 1337 597 787 268 1524
Fragments arranged by size:
1524 1337 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2058 2144 2058
Size: 86 4820
EcoRI G'AATT_C
Cuts at: 2215 2593 2215
Size: 378 4528
FspI TGC'GCA
Cuts at: 3412 3412
Size: 4906
HaeII r_GCGC'y
Cuts at: 1678 4053 1678
Size: 2375 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2002 2034 3547 3579 3621
Size: 32 135 32 430 32 1513 32 42
Cuts at: 3621 3653 1373
Size: 32 2626
Fragments arranged by size:
2626 1513 430 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2250 2496 669
Size: 988 281 312 246 3079
Fragments arranged by size:
3079 988 312 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 4906
HpaI GTT'AAC
Cuts at: 2496 2496
Size: 4906
KpnI G_GTAC'C
Cuts at: 2231 2231
Size: 4906
MfeI C'AATT_G
Cuts at: 2505 2505
Size: 4906
MluI A'CGCG_T
Cuts at: 2174 4782 2174
Size: 2608 2298
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4020 4204 1305
Size: 2715 184 2007
Fragments arranged by size:
2715 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3084
Fragments arranged by size:
3084 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2864 3223 3382 518
Size: 648 1698 359 159 2042
Fragments arranged by size:
2042 1698 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2136 2322 2999 4147 4392 1606
Size: 530 186 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 530 245 186
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4354
NdeI CA'TA_TG
Cuts at: 387 387
Size: 4906
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 4906
NotI GC'GGCC_GC
Cuts at: 2353 2353
Size: 4906
NspI r_CATG'y
Cuts at: 1132 1229 2264 3809 1132
Size: 97 1035 1545 2229
Fragments arranged by size:
2229 1545 1035 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 4906
PciI A'CATG_T
Cuts at: 3805 3805
Size: 4906
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2066 1108
Size: 958 3948
PmeI GTTT'AAAC
Cuts at: 2210 2210
Size: 4906
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2975 3007 4488 4520 2975
Size: 32 1481 32 3361
Fragments arranged by size:
3361 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 4906
PsiI TTA'TAA
Cuts at: 2476 2476
Size: 4906
PstI C_TGCA'G
Cuts at: 830 2258 830
Size: 1428 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 4906
PvuII CAG'CTG
Cuts at: 2136 2136
Size: 4906
SalI G'TCGA_C
Cuts at: 1936 2248 1936
Size: 312 4594
SapI GCTCTTCn'nnn_
Cuts at: 2042 2042
Size: 4906
SbfI CC_TGCA'GG
Cuts at: 2258 2258
Size: 4906
ScaI AGT'ACT
Cuts at: 3154 4866 3154
Size: 1712 3194
SfcI C'TryA_G
Cuts at: 826 1045 2254 3389 4070 4261 826
Size: 219 1209 1135 681 191 1471
Fragments arranged by size:
1471 1209 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4906
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 4906
SmaI CCC'GGG
Cuts at: 1872 2233 1872
Size: 361 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3010 3911 4173 820
Size: 197 926 1067 901 262 1553
Fragments arranged by size:
1553 1067 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2697 493
Size: 2204 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 4906
SphI G_CATG'C
Cuts at: 1132 2264 1132
Size: 1132 3774
SspI AAT'ATT
Cuts at: 5 52 2096 2830 5
Size: 47 2044 734 2081
Fragments arranged by size:
2081 2044 734 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 4906
StyI C'CwwG_G
Cuts at: 513 1065 2345 513
Size: 552 1280 3074
Fragments arranged by size:
3074 1280 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2933 2950 3103 3288 3733 325
Size: 1255 1353 17 153 185 445 1498
Fragments arranged by size:
1498 1353 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3152 4864 96
Size: 274 80 33 51 1443 1175 1712 138
Fragments arranged by size:
1712 1443 1175 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4866 4898 4866
Size: 32 4874
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 4906
XbaI T'CTAG_A
Cuts at: 2242 2242
Size: 4906
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 4906
XmaI C'CCGG_G
Cuts at: 1870 2231 1870
Size: 361 4545
XmnI GAAnn'nnTTC
Cuts at: 3035 3035
Size: 4906
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2714 276
Size: 53 83 186 936 1180 2468
Fragments arranged by size:
2468 1180 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI PvuII SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SexAI SfiI SfoI SrfI SwaI XcmI