Restriction map for
pFN21AE1395
(Circular) (Six-base) MAPSORT of: pFN21AE1395.seq Check: 9183 from: 1 to: 5785
pFN21AE1395 5785 bp
With 182 enzymes: SgfI *
August 16, 2010 14:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3595 278
Size: 53 83 186 936 2059 2468
Fragments arranged by size:
2468 2059 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3128 1937
Size: 1191 4594
Acc65I G'GTAC_C
Cuts at: 3106 3106
Size: 5785
AclI AA'CG_TT
Cuts at: 1563 3912 4285 1563
Size: 2349 373 3063
Fragments arranged by size:
3063 2349 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2668 3848 5232 5500 1632
Size: 1036 1180 1384 268 1917
Fragments arranged by size:
1917 1384 1180 1036 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5588
AflIII A'CryG_T
Cuts at: 1203 4684 5661 1203
Size: 3481 977 1327
Fragments arranged by size:
3481 1327 977
AgeI A'CCGG_T
Cuts at: 4616 4616
Size: 5785
AhdI GACnn_n'nnGTC
Cuts at: 4514 4514
Size: 5785
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5785
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5785
AlwNI CAG_nnn'CTG
Cuts at: 2382 3528 5100 2382
Size: 1146 1572 3067
Fragments arranged by size:
3067 1572 1146
ApaLI G'TGCA_C
Cuts at: 3038 3842 4998 3038
Size: 804 1156 3825
Fragments arranged by size:
3825 1156 804
ApoI r'AATT_y
Cuts at: 1493 2501 2821 3094 3320 3472 5581 1493
Size: 1008 320 273 226 152 2109 1697
Fragments arranged by size:
2109 1697 1008 320 273 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4339 160
Size: 872 3307 1606
Fragments arranged by size:
3307 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5785
AvaI C'yCGr_G
Cuts at: 1870 1943 2066 2898 3110 1870
Size: 73 123 832 212 4545
Fragments arranged by size:
4545 832 212 123 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2821 2854 2821
Size: 33 5752
BamHI G'GATC_C
Cuts at: 3115 3482 3115
Size: 367 5418
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3106 4561 618
Size: 325 217 627 1319 1455 1842
Fragments arranged by size:
1842 1455 1319 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2557 3104 1391
Size: 1166 547 4072
Fragments arranged by size:
4072 1166 547
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4814
BbvCI CC'TCA_GC
Cuts at: 2350 2350
Size: 5785
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3976 4010 1903
Size: 34 2039 34 3678
Fragments arranged by size:
3678 2039 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3677 4887 3677
Size: 1210 4575
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5785
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2295 4396 136
Size: 107 122 71 631 605 623 2101 1525
Fragments arranged by size:
2101 1525 631 623 605 122 107 71
BglII A'GATC_T
Cuts at: 5780 5780
Size: 5785
BlpI GC'TnA_GC
Cuts at: 3202 3202
Size: 5785
Bme1580I G_kGCm'C
Cuts at: 1790 3042 3846 5002 1790
Size: 1252 804 1156 2573
Fragments arranged by size:
2573 1252 1156 804
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5785
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3516 4474 442
Size: 416 53 83 399 24 2099 958 1753
Fragments arranged by size:
2099 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5785
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2092 2875 2092
Size: 783 5002
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2098 2376 2728 2811 4445 1239
Size: 859 278 352 83 1634 2579
Fragments arranged by size:
2579 1634 859 352 278 83
Bpu10I CC'TnA_GC
Cuts at: 2350 2350
Size: 5785
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3910 4775 5073 3910
Size: 865 298 4622
Fragments arranged by size:
4622 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4448 882
Size: 617 2949 2219
Fragments arranged by size:
2949 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3576 493
Size: 711 2372 2702
Fragments arranged by size:
2702 2372 711
BsaBI GATnn'nnATC
Cuts at: 5779 5779
Size: 5785
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3592 3974 4609 275
Size: 53 83 186 936 2059 382 635 1451
Fragments arranged by size:
2059 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4218 4616 4890 5037 5448 4218
Size: 398 274 147 411 4555
Fragments arranged by size:
4555 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2933 2963 1178
Size: 30 165 30 1530 30 4000
Fragments arranged by size:
4000 1530 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2101 2338 1186
Size: 915 237 4633
Fragments arranged by size:
4633 915 237
BseYI C'CCAG_C
Cuts at: 4988 5484 4988
Size: 496 5289
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5212
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3235 3996 5024 1540
Size: 275 186 1234 761 1028 2301
Fragments arranged by size:
2301 1234 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3042 3846 3931 5002 3042
Size: 804 85 1071 3825
Fragments arranged by size:
3825 1071 804 85
BsmI GAATG_Cn'
Cuts at: 2234 3296 3389 2234
Size: 1062 93 4630
Fragments arranged by size:
4630 1062 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2557 3042 3104 3846 3931 5002 1391
Size: 399 767 485 62 742 85 1071 2174
Fragments arranged by size:
2174 1071 767 742 485 399 85 62
BspEI T'CCGG_A
Cuts at: 5448 5448
Size: 5785
BspHI T'CATG_A
Cuts at: 2739 3674 5404 2739
Size: 935 1730 3120
Fragments arranged by size:
3120 1730 935
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3140 844
Size: 2296 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2640 3672 1394
Size: 429 817 1032 3507
Fragments arranged by size:
3507 1032 817 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4280 4454 65
Size: 1183 3032 174 1396
Fragments arranged by size:
3032 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4429 4616 1700
Size: 110 2619 187 2869
Fragments arranged by size:
2869 2619 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5785
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5785
BssSI C'ACGA_G
Cuts at: 2807 3845 4857 2807
Size: 1038 1012 3735
Fragments arranged by size:
3735 1038 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5785
BstBI TT'CG_AA
Cuts at: 4589 4589
Size: 5785
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5785
BstXI CCAn_nnnn'nTGG
Cuts at: 2734 2734
Size: 5785
BstYI r'GATC_y
Cuts at: 1972 3115 3482 3867 3884 5336 5422 5434 5780
Size: 1143 367 385 17 1452 86 12 346
Cuts at: 5780 1972
Size: 1977
Fragments arranged by size:
1977 1452 1143 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2629 2673 513
Size: 552 1564 44 3625
Fragments arranged by size:
3625 1564 552 44
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5046
Fragments arranged by size:
5046 683 56
BtsI GCAGTG_nn'
Cuts at: 2482 3301 4095 4115 2482
Size: 819 794 20 4152
Fragments arranged by size:
4152 819 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5785
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5750
DraI TTT'AAA
Cuts at: 3089 3089
Size: 5785
DraIII CAC_nnn'GTG
Cuts at: 2635 2635
Size: 5785
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4792 809
Size: 507 3476 1802
Fragments arranged by size:
3476 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2211 2670 2676
Size: 54 1425 50 275 18 381 459 6
Cuts at: 2676 3232 4121 5750 8
Size: 556 889 1629 43
Fragments arranged by size:
1629 1425 889 556 459 381 275 54
50 43 18 6
EagI C'GGCC_G
Cuts at: 1537 1812 3232 1537
Size: 275 1420 4090
Fragments arranged by size:
4090 1420 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2083 3715 1353
Size: 730 1632 3423
Fragments arranged by size:
3423 1632 730
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2234 4372 4746 4892 387
Size: 1064 138 645 2138 374 146 1280
Fragments arranged by size:
2138 1280 1064 645 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2098 2376 2668 2728 2811 3848 4445
Size: 393 466 278 292 60 83 1037 597
Cuts at: 4445 5232 5500 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1037 787 597 466 393 292 278
268 83 60
EcoO109I rG'GnC_Cy
Cuts at: 2302 2379 3052 2302
Size: 77 673 5035
Fragments arranged by size:
5035 673 77
EcoRI G'AATT_C
Cuts at: 2821 3094 3472 2821
Size: 273 378 5134
Fragments arranged by size:
5134 378 273
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2623 2623
Size: 5785
FspI TGC'GCA
Cuts at: 4291 4291
Size: 5785
HaeII r_GCGC'y
Cuts at: 1678 2439 4932 1678
Size: 761 2493 2531
Fragments arranged by size:
2531 2493 761
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4426 4458 4500 4532 1373
Size: 32 135 32 2854 32 42 32 2626
Fragments arranged by size:
2854 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2074 3129 3375 669
Size: 988 281 136 1055 246 3079
Fragments arranged by size:
3079 1055 988 281 246 136
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5785
HpaI GTT'AAC
Cuts at: 3375 3375
Size: 5785
KpnI G_GTAC'C
Cuts at: 3110 3110
Size: 5785
MfeI C'AATT_G
Cuts at: 3384 3384
Size: 5785
MluI A'CGCG_T
Cuts at: 5661 5661
Size: 5785
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4899 5083 1305
Size: 3594 184 2007
Fragments arranged by size:
3594 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2672 10
Size: 54 1425 343 840 3123
Fragments arranged by size:
3123 1425 840 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2181 2732 2963 3743 4102 4261 518
Size: 648 1015 551 231 780 359 159 2042
Fragments arranged by size:
2042 1015 780 648 551 359 231 159
MspA1I CmG'CkG
Cuts at: 1606 2158 2164 2256 2354 2913 3201 3878 5026
Size: 552 6 92 98 559 288 677 1148
Cuts at: 5026 5271 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 559 552 288 245 98
92 6
NcoI C'CATG_G
Cuts at: 513 1065 2673 513
Size: 552 1608 3625
Fragments arranged by size:
3625 1608 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5785
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5785
NotI GC'GGCC_GC
Cuts at: 3232 3232
Size: 5785
NspI r_CATG'y
Cuts at: 1132 1229 2664 3048 3143 4688 1132
Size: 97 1435 384 95 1545 2229
Fragments arranged by size:
2229 1545 1435 384 97 95
PasI CC'CwG_GG
Cuts at: 1348 2863 1348
Size: 1515 4270
PciI A'CATG_T
Cuts at: 4684 4684
Size: 5785
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2209 1108
Size: 1101 4684
PmeI GTTT'AAAC
Cuts at: 3089 3089
Size: 5785
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3854 3886 5367 5399 3854
Size: 32 1481 32 4240
Fragments arranged by size:
4240 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2302 2302
Size: 5785
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5785
PsiI TTA'TAA
Cuts at: 3355 3355
Size: 5785
PstI C_TGCA'G
Cuts at: 830 2477 3137 830
Size: 1647 660 3478
Fragments arranged by size:
3478 1647 660
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5785
PvuII CAG'CTG
Cuts at: 2164 2354 2913 2164
Size: 190 559 5036
Fragments arranged by size:
5036 559 190
SalI G'TCGA_C
Cuts at: 1936 3127 1936
Size: 1191 4594
SbfI CC_TGCA'GG
Cuts at: 3137 3137
Size: 5785
ScaI AGT'ACT
Cuts at: 4033 5745 4033
Size: 1712 4073
SfcI C'TryA_G
Cuts at: 826 1045 2100 2383 2473 3081 3133 4268 4949
Size: 219 1055 283 90 608 52 1135 681
Cuts at: 4949 5140 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1055 681 608 283 219 191
90 52
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5785
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5785
SmaI CCC'GGG
Cuts at: 1872 2900 3112 1872
Size: 1028 212 4545
Fragments arranged by size:
4545 1028 212
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3889 4790 5052 820
Size: 197 926 1946 901 262 1553
Fragments arranged by size:
1946 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3576 493
Size: 3083 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5785
SphI G_CATG'C
Cuts at: 1132 3143 1132
Size: 2011 3774
SspI AAT'ATT
Cuts at: 5 52 3709 5
Size: 47 3657 2081
Fragments arranged by size:
3657 2081 47
StuI AGG'CCT
Cuts at: 1517 2142 1517
Size: 625 5160
StyI C'CwwG_G
Cuts at: 513 1065 2389 2439 2673 3224 513
Size: 552 1324 50 234 551 3074
Fragments arranged by size:
3074 1324 552 551 234 50
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2862 2967 3812 3829 3982 4167 4612
Size: 1255 1282 105 845 17 153 185 445
Cuts at: 4612 325
Size: 1498
Fragments arranged by size:
1498 1282 1255 845 445 185 153 105
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2648 2715 4031
Size: 274 80 33 51 1443 671 67 1316
Cuts at: 4031 5743 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1316 671 274 138 80 67
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5745 5777 5745
Size: 32 5753
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5785
XbaI T'CTAG_A
Cuts at: 3121 3121
Size: 5785
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5785
XmaI C'CCGG_G
Cuts at: 1870 2898 3110 1870
Size: 1028 212 4545
Fragments arranged by size:
4545 1028 212
XmnI GAAnn'nnTTC
Cuts at: 3914 3914
Size: 5785
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3593 276
Size: 53 83 186 936 2059 2468
Fragments arranged by size:
2468 2059 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI
BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI