Restriction map for
pFN21AE1394
(Circular) (Six-base) MAPSORT of: pFN21AE1394.seq Check: 2938 from: 1 to: 6271
pFN21AE1394 6271 bp
With 182 enzymes: SgfI *
January 28, 2010 16:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2677 3157 2677
Size: 480 5791
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4081 278
Size: 53 83 186 936 2545 2468
Fragments arranged by size:
2545 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3614 1937
Size: 1677 4594
Acc65I G'GTAC_C
Cuts at: 3592 3592
Size: 6271
AclI AA'CG_TT
Cuts at: 1563 4398 4771 1563
Size: 2835 373 3063
Fragments arranged by size:
3063 2835 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3069 3076 3261 3476 4334 5718 5986 1632
Size: 1437 7 185 215 858 1384 268 1917
Fragments arranged by size:
1917 1437 1384 858 268 215 185 7
AflII C'TTAA_G
Cuts at: 820 1017 2521 820
Size: 197 1504 4570
Fragments arranged by size:
4570 1504 197
AflIII A'CryG_T
Cuts at: 1203 5170 6147 1203
Size: 3967 977 1327
Fragments arranged by size:
3967 1327 977
AgeI A'CCGG_T
Cuts at: 5102 5102
Size: 6271
AhdI GACnn_n'nnGTC
Cuts at: 5000 5000
Size: 6271
AleI CACnn'nnGTG
Cuts at: 1166 2712 1166
Size: 1546 4725
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6271
AlwNI CAG_nnn'CTG
Cuts at: 2678 4014 5586 2678
Size: 1336 1572 3363
Fragments arranged by size:
3363 1572 1336
ApaLI G'TGCA_C
Cuts at: 4328 5484 4328
Size: 1156 5115
ApoI r'AATT_y
Cuts at: 1493 2051 2157 3307 3580 3806 3958 6067 1493
Size: 558 106 1150 273 226 152 2109 1697
Fragments arranged by size:
2109 1697 1150 558 273 226 152 106
AseI AT'TA_AT
Cuts at: 160 1032 4825 160
Size: 872 3793 1606
Fragments arranged by size:
3793 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6271
AvaI C'yCGr_G
Cuts at: 1870 1943 3596 1870
Size: 73 1653 4545
Fragments arranged by size:
4545 1653 73
BamHI G'GATC_C
Cuts at: 2202 2722 3601 3968 2202
Size: 520 879 367 4505
Fragments arranged by size:
4505 879 520 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3592 5047 618
Size: 325 217 627 1805 1455 1842
Fragments arranged by size:
1842 1805 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3590 1391
Size: 2199 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2604 3015 928
Size: 971 705 411 4184
Fragments arranged by size:
4184 971 705 411
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4462 4496 1903
Size: 34 2525 34 3678
Fragments arranged by size:
3678 2525 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4163 5373 4163
Size: 1210 5061
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6271
BfrBI ATG'CAT
Cuts at: 2104 2104
Size: 6271
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2252 4882 136
Size: 107 122 71 631 605 580 2630 1525
Fragments arranged by size:
2630 1525 631 605 580 122 107 71
BglII A'GATC_T
Cuts at: 2489 6266 2489
Size: 3777 2494
BlpI GC'TnA_GC
Cuts at: 3688 3688
Size: 6271
Bme1580I G_kGCm'C
Cuts at: 1790 4332 5488 1790
Size: 2542 1156 2573
Fragments arranged by size:
2573 2542 1156
BmgBI CAC'GTC
Cuts at: 1318 2926 1318
Size: 1608 4663
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2759 4002 4960
Size: 416 53 83 399 24 1342 1243 958
Cuts at: 4960 442
Size: 1753
Fragments arranged by size:
1753 1342 1243 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6271
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2172 3260 4931 1239
Size: 933 1088 1671 2579
Fragments arranged by size:
2579 1671 1088 933
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4396 5261 5559 4396
Size: 865 298 5108
Fragments arranged by size:
5108 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4934 882
Size: 617 3435 2219
Fragments arranged by size:
3435 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2689 4062 493
Size: 711 1485 1373 2702
Fragments arranged by size:
2702 1485 1373 711
BsaBI GATnn'nnATC
Cuts at: 2201 6265 2201
Size: 4064 2207
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4078 4460 5095 275
Size: 53 83 186 936 2545 382 635 1451
Fragments arranged by size:
2545 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4704 5102 5376 5523 5934 4704
Size: 398 274 147 411 5041
Fragments arranged by size:
5041 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2170 2200 2292 2322 1178
Size: 30 165 30 767 30 92 30 5127
Fragments arranged by size:
5127 767 165 92 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2175 2903 3257 1186
Size: 989 728 354 4200
Fragments arranged by size:
4200 989 728 354
BseYI C'CCAG_C
Cuts at: 5474 5970 5474
Size: 496 5775
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5698
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3721 4482 5510 1540
Size: 275 186 1720 761 1028 2301
Fragments arranged by size:
2301 1720 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3148 4332 4417 5488 3148
Size: 1184 85 1071 3931
Fragments arranged by size:
3931 1184 1071 85
BsmI GAATG_Cn'
Cuts at: 3782 3875 3782
Size: 93 6178
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3148 3590 4332 4417 5488 1391
Size: 399 1358 442 742 85 1071 2174
Fragments arranged by size:
2174 1358 1071 742 442 399 85
BspEI T'CCGG_A
Cuts at: 5934 5934
Size: 6271
BspHI T'CATG_A
Cuts at: 2757 4160 5890 2757
Size: 1403 1730 3138
Fragments arranged by size:
3138 1730 1403
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2677 3157 3626 844
Size: 1833 480 469 3489
Fragments arranged by size:
3489 1833 480 469
BsrBI CCG'CTC
Cuts at: 1394 1823 4158 1394
Size: 429 2335 3507
Fragments arranged by size:
3507 2335 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2086 2232 2451 2736 3499 4766 4940
Size: 1183 838 146 219 285 763 1267 174
Cuts at: 4940 65
Size: 1396
Fragments arranged by size:
1396 1267 1183 838 763 285 219 174
146
BsrFI r'CCGG_y
Cuts at: 1700 1810 3040 4915 5102 1700
Size: 110 1230 1875 187 2869
Fragments arranged by size:
2869 1875 1230 187 110
BsrGI T'GTAC_A
Cuts at: 96 3297 96
Size: 3201 3070
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6271
BssSI C'ACGA_G
Cuts at: 4331 5343 4331
Size: 1012 5259
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6271
BstBI TT'CG_AA
Cuts at: 5075 5075
Size: 6271
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6271
BstXI CCAn_nnnn'nTGG
Cuts at: 2749 2749
Size: 6271
BstYI r'GATC_y
Cuts at: 1972 2202 2489 2618 2722 3470 3601 3968 4353
Size: 230 287 129 104 748 131 367 385
Cuts at: 4353 4370 5822 5908 5920 6266 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 748 385 367 346 287 230
131 129 104 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2433 2970 513
Size: 552 1368 537 3814
Fragments arranged by size:
3814 1368 552 537
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5532
Fragments arranged by size:
5532 683 56
BtsI GCAGTG_nn'
Cuts at: 3787 4581 4601 3787
Size: 794 20 5457
Fragments arranged by size:
5457 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6271
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6236
DraI TTT'AAA
Cuts at: 3575 3575
Size: 6271
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5278 809
Size: 507 3962 1802
Fragments arranged by size:
3962 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2430 3718 4607
Size: 54 1425 50 275 18 600 1288 889
Cuts at: 4607 6236 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1288 889 600 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3718 1537
Size: 275 1906 4090
Fragments arranged by size:
4090 1906 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2986 3154 3414 4201 1353
Size: 1633 168 260 787 3423
Fragments arranged by size:
3423 1633 787 260 168
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4858 5232 5378 387
Size: 1064 138 3269 374 146 1280
Fragments arranged by size:
3269 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2172 3069 3076 3260 3261 3476 4334
Size: 393 540 897 7 184 1 215 858
Cuts at: 4334 4931 5718 5986 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 897 858 787 597 540 393 268
215 184 7 1
EcoNI CCTnn'n_nnAGG
Cuts at: 2322 2322
Size: 6271
EcoRI G'AATT_C
Cuts at: 2051 3580 3958 2051
Size: 1529 378 4364
Fragments arranged by size:
4364 1529 378
FspI TGC'GCA
Cuts at: 4777 4777
Size: 6271
HaeII r_GCGC'y
Cuts at: 1678 5418 1678
Size: 3740 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2103 2135 2170 2202 4912
Size: 32 135 32 531 32 35 32 2710
Cuts at: 4912 4944 4986 5018 1373
Size: 32 42 32 2626
Fragments arranged by size:
2710 2626 531 135 42 35 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2962 3615 3861 669
Size: 988 281 1024 653 246 3079
Fragments arranged by size:
3079 1024 988 653 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6271
HpaI GTT'AAC
Cuts at: 3861 3861
Size: 6271
KpnI G_GTAC'C
Cuts at: 3596 3596
Size: 6271
MfeI C'AATT_G
Cuts at: 2574 3870 2574
Size: 1296 4975
MluI A'CGCG_T
Cuts at: 6147 6147
Size: 6271
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3526 5385 5569 1305
Size: 2221 1859 184 2007
Fragments arranged by size:
2221 2007 1859 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2432 10
Size: 54 1425 343 600 3849
Fragments arranged by size:
3849 1425 600 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2222 2558 2712 2862 4229 4588 4747
Size: 648 1056 336 154 150 1367 359 159
Cuts at: 4747 518
Size: 2042
Fragments arranged by size:
2042 1367 1056 648 359 336 159 154
150
MspA1I CmG'CkG
Cuts at: 1606 3114 3687 4364 5512 5757 1606
Size: 1508 573 677 1148 245 2120
Fragments arranged by size:
2120 1508 1148 677 573 245
NcoI C'CATG_G
Cuts at: 513 1065 2433 2970 513
Size: 552 1368 537 3814
Fragments arranged by size:
3814 1368 552 537
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6271
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6271
NotI GC'GGCC_GC
Cuts at: 3718 3718
Size: 6271
NsiI A_TGCA'T
Cuts at: 2106 2106
Size: 6271
NspI r_CATG'y
Cuts at: 1132 1229 2108 3629 5174 1132
Size: 97 879 1521 1545 2229
Fragments arranged by size:
2229 1545 1521 879 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6271
PciI A'CATG_T
Cuts at: 5170 5170
Size: 6271
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6271
PfoI T'CCnGG_A
Cuts at: 2188 2188
Size: 6271
PmeI GTTT'AAAC
Cuts at: 3575 3575
Size: 6271
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4340 4372 5853 5885 4340
Size: 32 1481 32 4726
Fragments arranged by size:
4726 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6271
PsiI TTA'TAA
Cuts at: 2486 3841 2486
Size: 1355 4916
PstI C_TGCA'G
Cuts at: 830 2674 2914 3478 3623 830
Size: 1844 240 564 145 3478
Fragments arranged by size:
3478 1844 564 240 145
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6271
SalI G'TCGA_C
Cuts at: 1936 3613 1936
Size: 1677 4594
SapI GCTCTTCn'nnn_
Cuts at: 3154 3154
Size: 6271
SbfI CC_TGCA'GG
Cuts at: 3623 3623
Size: 6271
ScaI AGT'ACT
Cuts at: 4519 6231 4519
Size: 1712 4559
SfcI C'TryA_G
Cuts at: 826 1045 2670 2910 3323 3403 3474 3619 4754
Size: 219 1625 240 413 80 71 145 1135
Cuts at: 4754 5435 5626 826
Size: 681 191 1471
Fragments arranged by size:
1625 1471 1135 681 413 240 219 191
145 80 71
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6271
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6271
SmaI CCC'GGG
Cuts at: 1872 3598 1872
Size: 1726 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2521 4375 5276 5538 820
Size: 197 926 578 1854 901 262 1553
Fragments arranged by size:
1854 1553 926 901 578 262 197
SnaBI TAC'GTA
Cuts at: 493 4062 493
Size: 3569 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6271
SphI G_CATG'C
Cuts at: 1132 3629 1132
Size: 2497 3774
SspI AAT'ATT
Cuts at: 5 52 2839 4195 5
Size: 47 2787 1356 2081
Fragments arranged by size:
2787 2081 1356 47
StuI AGG'CCT
Cuts at: 1517 2017 2044 1517
Size: 500 27 5744
Fragments arranged by size:
5744 500 27
StyI C'CwwG_G
Cuts at: 513 1065 2433 2970 3710 513
Size: 552 1368 537 740 3074
Fragments arranged by size:
3074 1368 740 552 537
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4298 4315 4468 4653 5098 325
Size: 1255 2718 17 153 185 445 1498
Fragments arranged by size:
2718 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2746 3297 3423
Size: 274 80 33 51 1443 769 551 126
Cuts at: 3423 4517 6229 96
Size: 1094 1712 138
Fragments arranged by size:
1712 1443 1094 769 551 274 138 126
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2673 2705 3028 3060 6231 6263 2673
Size: 32 323 32 3171 32 2681
Fragments arranged by size:
3171 2681 323 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6271
XbaI T'CTAG_A
Cuts at: 3607 3607
Size: 6271
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6271
XmaI C'CCGG_G
Cuts at: 1870 3596 1870
Size: 1726 4545
XmnI GAAnn'nnTTC
Cuts at: 2160 3054 4400 2160
Size: 894 1346 4031
Fragments arranged by size:
4031 1346 894
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4079 276
Size: 53 83 186 936 2545 2468
Fragments arranged by size:
2545 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI
PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI
SrfI SwaI XcmI