Restriction map for
pFN21AE1386
(Circular) (Six-base) MAPSORT of: pFN21AE1386.seq Check: 3492 from: 1 to: 5320
pFN21AE1386 5320 bp
With 182 enzymes: SgfI *
August 16, 2010 14:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3130 278
Size: 53 83 186 936 1594 2468
Fragments arranged by size:
2468 1594 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2331 2663 1937
Size: 394 332 4594
Fragments arranged by size:
4594 394 332
Acc65I G'GTAC_C
Cuts at: 2641 2641
Size: 5320
AclI AA'CG_TT
Cuts at: 1563 3447 3820 1563
Size: 1884 373 3063
Fragments arranged by size:
3063 1884 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2115 3383 4767 5035 1632
Size: 483 1268 1384 268 1917
Fragments arranged by size:
1917 1384 1268 483 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5123
AflIII A'CryG_T
Cuts at: 1203 4219 5196 1203
Size: 3016 977 1327
Fragments arranged by size:
3016 1327 977
AgeI A'CCGG_T
Cuts at: 4151 4151
Size: 5320
AhdI GACnn_n'nnGTC
Cuts at: 2148 4049 2148
Size: 1901 3419
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5320
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5320
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2010 2042 2010
Size: 32 5288
AlwNI CAG_nnn'CTG
Cuts at: 3063 4635 3063
Size: 1572 3748
ApaI G_GGCC'C
Cuts at: 2101 2101
Size: 5320
ApaLI G'TGCA_C
Cuts at: 3377 4533 3377
Size: 1156 4164
ApoI r'AATT_y
Cuts at: 1493 2629 2855 3007 5116 1493
Size: 1136 226 152 2109 1697
Fragments arranged by size:
2109 1697 1136 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3874 160
Size: 872 2842 1606
Fragments arranged by size:
2842 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5320
AvaI C'yCGr_G
Cuts at: 1870 1943 2645 1870
Size: 73 702 4545
Fragments arranged by size:
4545 702 73
BamHI G'GATC_C
Cuts at: 2650 3017 2650
Size: 367 4953
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2045 2641 4096 618
Size: 325 217 627 258 596 1455 1842
Fragments arranged by size:
1842 1455 627 596 325 258 217
BanII G_rGCy'C
Cuts at: 1391 2101 2109 2639 1391
Size: 710 8 530 4072
Fragments arranged by size:
4072 710 530 8
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2338 2590 928
Size: 971 439 252 3658
Fragments arranged by size:
3658 971 439 252
BbvCI CC'TCA_GC
Cuts at: 2450 2582 2450
Size: 132 5188
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2308 2342 3511 3545 1903
Size: 34 371 34 1169 34 3678
Fragments arranged by size:
3678 1169 371 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2481 3212 4422 2481
Size: 731 1210 3379
Fragments arranged by size:
3379 1210 731
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5320
BfrBI ATG'CAT
Cuts at: 2167 2167
Size: 5320
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3931 136
Size: 107 122 71 631 605 2259 1525
Fragments arranged by size:
2259 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5315 5315
Size: 5320
BlpI GC'TnA_GC
Cuts at: 2737 2737
Size: 5320
Bme1580I G_kGCm'C
Cuts at: 1790 2101 3381 4537 1790
Size: 311 1280 1156 2573
Fragments arranged by size:
2573 1280 1156 311
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5320
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2419 3051 4009
Size: 416 53 83 399 24 1002 632 958
Cuts at: 4009 442
Size: 1753
Fragments arranged by size:
1753 1002 958 632 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5320
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2252 2260 3980 1239
Size: 1013 8 1720 2579
Fragments arranged by size:
2579 1720 1013 8
Bpu10I CC'TnA_GC
Cuts at: 2450 2582 2450
Size: 132 5188
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2305 3445 4310 4608 2305
Size: 1140 865 298 3017
Fragments arranged by size:
3017 1140 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2205 2532 3983 882
Size: 617 706 327 1451 2219
Fragments arranged by size:
2219 1451 706 617 327
BsaAI yAC'GTr
Cuts at: 493 1204 3111 493
Size: 711 1907 2702
Fragments arranged by size:
2702 1907 711
BsaBI GATnn'nnATC
Cuts at: 5314 5314
Size: 5320
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3127 3509 4144 275
Size: 53 83 186 936 1594 382 635 1451
Fragments arranged by size:
1594 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3753 4151 4425 4572 4983 3753
Size: 398 274 147 411 4090
Fragments arranged by size:
4090 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2046 2076 1178
Size: 30 165 30 643 30 4422
Fragments arranged by size:
4422 643 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2051 2371 2410 2438 1186
Size: 865 320 39 28 4068
Fragments arranged by size:
4068 865 320 39 28
BseYI C'CCAG_C
Cuts at: 2216 4523 5019 2216
Size: 2307 496 2517
Fragments arranged by size:
2517 2307 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2480 1163
Size: 573 744 4003
Fragments arranged by size:
4003 744 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2770 3531 4559 1540
Size: 275 186 769 761 1028 2301
Fragments arranged by size:
2301 1028 769 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3381 3466 4537 3381
Size: 85 1071 4164
Fragments arranged by size:
4164 1071 85
BsmI GAATG_Cn'
Cuts at: 2831 2924 2831
Size: 93 5227
BsmBI CGTCTCn'nnnn_
Cuts at: 2027 2027
Size: 5320
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2101 2109 2639 3381 3466 4537 1391
Size: 399 311 8 530 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 530 399 311 85 8
BspEI T'CCGG_A
Cuts at: 4983 4983
Size: 5320
BspHI T'CATG_A
Cuts at: 3209 4939 3209
Size: 1730 3590
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2200 2675 844
Size: 1356 475 3489
Fragments arranged by size:
3489 1356 475
BsrBI CCG'CTC
Cuts at: 1394 1823 3207 1394
Size: 429 1384 3507
Fragments arranged by size:
3507 1384 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3815 3989 65
Size: 1183 2567 174 1396
Fragments arranged by size:
2567 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3964 4151 1700
Size: 110 2154 187 2869
Fragments arranged by size:
2869 2154 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5320
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5320
BssSI C'ACGA_G
Cuts at: 3380 4392 3380
Size: 1012 4308
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5320
BstBI TT'CG_AA
Cuts at: 4124 4124
Size: 5320
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5320
BstXI CCAn_nnnn'nTGG
Cuts at: 2561 2561
Size: 5320
BstYI r'GATC_y
Cuts at: 1972 2650 3017 3402 3419 4871 4957 4969 5315
Size: 678 367 385 17 1452 86 12 346
Cuts at: 5315 1972
Size: 1977
Fragments arranged by size:
1977 1452 678 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2509 513
Size: 552 1444 3324
Fragments arranged by size:
3324 1444 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4581
Fragments arranged by size:
4581 683 56
BtsI GCAGTG_nn'
Cuts at: 2231 2836 3630 3650 2231
Size: 605 794 20 3901
Fragments arranged by size:
3901 794 605 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5320
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5285
DraI TTT'AAA
Cuts at: 2624 2624
Size: 5320
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4327 809
Size: 507 3011 1802
Fragments arranged by size:
3011 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2767 3656 5285
Size: 54 1425 50 275 18 937 889 1629
Cuts at: 5285 8
Size: 43
Fragments arranged by size:
1629 1425 937 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2767 1537
Size: 275 955 4090
Fragments arranged by size:
4090 955 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2030 2420 3250 1353
Size: 677 390 830 3423
Fragments arranged by size:
3423 830 677 390
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3907 4281 4427 387
Size: 1064 138 2318 374 146 1280
Fragments arranged by size:
2318 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2115 2252 2260 3383 3980 4767 5035
Size: 393 483 137 8 1123 597 787 268
Cuts at: 5035 1239
Size: 1524
Fragments arranged by size:
1524 1123 787 597 483 393 268 137
8
EcoO109I rG'GnC_Cy
Cuts at: 2097 2097
Size: 5320
EcoRI G'AATT_C
Cuts at: 2629 3007 2629
Size: 378 4942
FspI TGC'GCA
Cuts at: 3826 3826
Size: 5320
HaeII r_GCGC'y
Cuts at: 1678 4467 1678
Size: 2789 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2134 2166 2264 2296 2455
Size: 32 135 32 562 32 98 32 159
Cuts at: 2455 2487 2531 2563 3961 3993 4035 4067 1373
Size: 32 44 32 1398 32 42 32 2626
Fragments arranged by size:
2626 1398 562 159 135 98 44 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2664 2910 669
Size: 988 281 726 246 3079
Fragments arranged by size:
3079 988 726 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5320
HpaI GTT'AAC
Cuts at: 2910 2910
Size: 5320
KpnI G_GTAC'C
Cuts at: 2645 2645
Size: 5320
MfeI C'AATT_G
Cuts at: 2919 2919
Size: 5320
MluI A'CGCG_T
Cuts at: 5196 5196
Size: 5320
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4434 4618 1305
Size: 3129 184 2007
Fragments arranged by size:
3129 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3498
Fragments arranged by size:
3498 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2559 3278 3637 3796 518
Size: 648 1393 719 359 159 2042
Fragments arranged by size:
2042 1393 719 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2135 2736 3413 4561 4806 1606
Size: 529 601 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 601 529 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4768
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5320
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5320
NotI GC'GGCC_GC
Cuts at: 2767 2767
Size: 5320
NsiI A_TGCA'T
Cuts at: 2169 2169
Size: 5320
NspI r_CATG'y
Cuts at: 1132 1229 2678 4223 1132
Size: 97 1449 1545 2229
Fragments arranged by size:
2229 1545 1449 97
PasI CC'CwG_GG
Cuts at: 1348 2101 2533 1348
Size: 753 432 4135
Fragments arranged by size:
4135 753 432
PciI A'CATG_T
Cuts at: 4219 4219
Size: 5320
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2464 1108
Size: 1356 3964
PfoI T'CCnGG_A
Cuts at: 2268 2268
Size: 5320
PmeI GTTT'AAAC
Cuts at: 2624 2624
Size: 5320
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3389 3421 4902 4934 3389
Size: 32 1481 32 3775
Fragments arranged by size:
3775 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5320
PsiI TTA'TAA
Cuts at: 2890 2890
Size: 5320
PspOMI G'GGCC_C
Cuts at: 2097 2097
Size: 5320
PstI C_TGCA'G
Cuts at: 830 2484 2672 830
Size: 1654 188 3478
Fragments arranged by size:
3478 1654 188
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5320
PvuII CAG'CTG
Cuts at: 2135 2135
Size: 5320
RsrII CG'GwC_CG
Cuts at: 2254 2254
Size: 5320
SalI G'TCGA_C
Cuts at: 1936 2662 1936
Size: 726 4594
SapI GCTCTTCn'nnn_
Cuts at: 2420 2420
Size: 5320
SbfI CC_TGCA'GG
Cuts at: 2672 2672
Size: 5320
ScaI AGT'ACT
Cuts at: 2593 3568 5280 2593
Size: 975 1712 2633
Fragments arranged by size:
2633 1712 975
SfcI C'TryA_G
Cuts at: 826 1045 2480 2668 3803 4484 4675 826
Size: 219 1435 188 1135 681 191 1471
Fragments arranged by size:
1471 1435 1135 681 219 191 188
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5320
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5320
SmaI CCC'GGG
Cuts at: 1872 2647 1872
Size: 775 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2284 3424 4325 4587 820
Size: 197 926 341 1140 901 262 1553
Fragments arranged by size:
1553 1140 926 901 341 262 197
SnaBI TAC'GTA
Cuts at: 493 3111 493
Size: 2618 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5320
SphI G_CATG'C
Cuts at: 1132 2678 1132
Size: 1546 3774
SspI AAT'ATT
Cuts at: 5 52 3244 5
Size: 47 3192 2081
Fragments arranged by size:
3192 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5320
StyI C'CwwG_G
Cuts at: 513 1065 2033 2370 2759 513
Size: 552 968 337 389 3074
Fragments arranged by size:
3074 968 552 389 337
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2546 2567 3347 3364 3517 3702 4147
Size: 1255 966 21 780 17 153 185 445
Cuts at: 4147 325
Size: 1498
Fragments arranged by size:
1498 1255 966 780 445 185 153 21
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2591 3566 5278
Size: 274 80 33 51 1443 614 975 1712
Cuts at: 5278 96
Size: 138
Fragments arranged by size:
1712 1443 975 614 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5280 5312 5280
Size: 32 5288
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5320
XbaI T'CTAG_A
Cuts at: 2656 2656
Size: 5320
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2108 2108
Size: 5320
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5320
XmaI C'CCGG_G
Cuts at: 1870 2645 1870
Size: 775 4545
XmnI GAAnn'nnTTC
Cuts at: 3449 3449
Size: 5320
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3128 276
Size: 53 83 186 936 1594 2468
Fragments arranged by size:
2468 1594 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PspOMI PstI PvuI PvuII RsrII
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BplI BsiWI
BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI
PpuMI PspXI PsrI SacI SacII SanDI SexAI SfiI
SfoI SrfI SwaI