Restriction map for
pFN21AE1379
(Circular) (Six-base) MAPSORT of: pFN21AE1379.seq Check: 5256 from: 1 to: 5464
pFN21AE1379 5464 bp
With 182 enzymes: SgfI *
August 16, 2010 14:46 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3274 278
Size: 53 83 186 936 1738 2468
Fragments arranged by size:
2468 1738 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2807 1937
Size: 870 4594
Acc65I G'GTAC_C
Cuts at: 2785 2785
Size: 5464
AclI AA'CG_TT
Cuts at: 1563 3591 3964 1563
Size: 2028 373 3063
Fragments arranged by size:
3063 2028 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2402 3527 4911 5179 1632
Size: 770 1125 1384 268 1917
Fragments arranged by size:
1917 1384 1125 770 268
AfeI AGC'GCT
Cuts at: 2402 2437 2402
Size: 35 5429
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5267
AflIII A'CryG_T
Cuts at: 1203 2033 4363 5340 1203
Size: 830 2330 977 1327
Fragments arranged by size:
2330 1327 977 830
AgeI A'CCGG_T
Cuts at: 4295 4295
Size: 5464
AhdI GACnn_n'nnGTC
Cuts at: 4193 4193
Size: 5464
AleI CACnn'nnGTG
Cuts at: 1166 2469 1166
Size: 1303 4161
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5464
AlwNI CAG_nnn'CTG
Cuts at: 2356 2556 3207 4779 2356
Size: 200 651 1572 3041
Fragments arranged by size:
3041 1572 651 200
ApaLI G'TGCA_C
Cuts at: 3521 4677 3521
Size: 1156 4308
ApoI r'AATT_y
Cuts at: 1493 2773 2999 3151 5260 1493
Size: 1280 226 152 2109 1697
Fragments arranged by size:
2109 1697 1280 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4018 160
Size: 872 2986 1606
Fragments arranged by size:
2986 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5464
AvaI C'yCGr_G
Cuts at: 1870 1943 2789 1870
Size: 73 846 4545
Fragments arranged by size:
4545 846 73
BamHI G'GATC_C
Cuts at: 2511 2794 3161 2511
Size: 283 367 4814
Fragments arranged by size:
4814 367 283
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2039 2168 2785 4240 618
Size: 325 217 627 252 129 617 1455 1842
Fragments arranged by size:
1842 1455 627 617 325 252 217 129
BanII G_rGCy'C
Cuts at: 1391 2370 2783 1391
Size: 979 413 4072
Fragments arranged by size:
4072 979 413
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2033 928
Size: 971 134 4359
Fragments arranged by size:
4359 971 134
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3655 3689 1903
Size: 34 1718 34 3678
Fragments arranged by size:
3678 1718 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2339 3356 4566 2339
Size: 1017 1210 3237
Fragments arranged by size:
3237 1210 1017
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5464
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4075 136
Size: 107 122 71 631 605 2403 1525
Fragments arranged by size:
2403 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5459 5459
Size: 5464
BlpI GC'TnA_GC
Cuts at: 2881 2881
Size: 5464
Bme1580I G_kGCm'C
Cuts at: 1790 2210 3525 4681 1790
Size: 420 1315 1156 2573
Fragments arranged by size:
2573 1315 1156 420
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5464
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2235 3195 4153
Size: 416 53 83 399 24 818 960 958
Cuts at: 4153 442
Size: 1753
Fragments arranged by size:
1753 960 958 818 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5464
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2333 2774 4124 1239
Size: 1094 441 1350 2579
Fragments arranged by size:
2579 1350 1094 441
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2036 2120 2354 2732 3589 4454 4752 2036
Size: 84 234 378 857 865 298 2748
Fragments arranged by size:
2748 865 857 378 298 234 84
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2583 2636 4127 882
Size: 617 1084 53 1491 2219
Fragments arranged by size:
2219 1491 1084 617 53
BsaAI yAC'GTr
Cuts at: 493 1204 2727 3255 493
Size: 711 1523 528 2702
Fragments arranged by size:
2702 1523 711 528
BsaBI GATnn'nnATC
Cuts at: 5458 5458
Size: 5464
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3271 3653 4288 275
Size: 53 83 186 936 1738 382 635 1451
Fragments arranged by size:
1738 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3897 4295 4569 4716 5127 3897
Size: 398 274 147 411 4234
Fragments arranged by size:
4234 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2582 2612 1178
Size: 30 165 30 1179 30 4030
Fragments arranged by size:
4030 1179 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2285 1186
Size: 1099 4365
BseYI C'CCAG_C
Cuts at: 4667 5163 4667
Size: 496 4968
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2723 1163
Size: 573 987 3904
Fragments arranged by size:
3904 987 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2914 3675 4703 1540
Size: 275 186 913 761 1028 2301
Fragments arranged by size:
2301 1028 913 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2370 2661 2760 3525 3610 4681 2370
Size: 291 99 765 85 1071 3153
Fragments arranged by size:
3153 1071 765 291 99 85
BsmI GAATG_Cn'
Cuts at: 2975 3068 2975
Size: 93 5371
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2210 2370 2661 2760 2783 3525 3610
Size: 399 420 160 291 99 23 742 85
Cuts at: 3610 4681 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 420 399 291 160 99
85 23
BspEI T'CCGG_A
Cuts at: 5127 5127
Size: 5464
BspHI T'CATG_A
Cuts at: 3353 5083 3353
Size: 1730 3734
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2067 2277 2695 2819 844
Size: 1223 210 418 124 3489
Fragments arranged by size:
3489 1223 418 210 124
BsrBI CCG'CTC
Cuts at: 1394 1823 2517 3351 1394
Size: 429 694 834 3507
Fragments arranged by size:
3507 834 694 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2691 3959 4133 65
Size: 1183 1443 1268 174 1396
Fragments arranged by size:
1443 1396 1268 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2528 4108 4295 1700
Size: 110 718 1580 187 2869
Fragments arranged by size:
2869 1580 718 187 110
BsrGI T'GTAC_A
Cuts at: 96 2736 96
Size: 2640 2824
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5464
BssSI C'ACGA_G
Cuts at: 3524 4536 3524
Size: 1012 4452
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2046 794
Size: 1252 4212
BstBI TT'CG_AA
Cuts at: 4268 4268
Size: 5464
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5464
BstXI CCAn_nnnn'nTGG
Cuts at: 2440 2471 2440
Size: 31 5433
BstYI r'GATC_y
Cuts at: 1972 2511 2794 3161 3546 3563 5015 5101 5113
Size: 539 283 367 385 17 1452 86 12
Cuts at: 5113 5459 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 539 385 367 346 283 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2164 2663 2164
Size: 499 4965
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3973
Fragments arranged by size:
3973 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2305 487
Size: 683 56 1079 3646
Fragments arranged by size:
3646 1079 683 56
BtsI GCAGTG_nn'
Cuts at: 2118 2464 2980 3774 3794 2118
Size: 346 516 794 20 3788
Fragments arranged by size:
3788 794 516 346 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5464
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5429
DraI TTT'AAA
Cuts at: 2768 2768
Size: 5464
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4471 809
Size: 507 3155 1802
Fragments arranged by size:
3155 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2178 2533 2911
Size: 54 1425 50 275 18 348 355 378
Cuts at: 2911 3800 5429 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 378 355 348 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2911 1537
Size: 275 1099 4090
Fragments arranged by size:
4090 1099 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3394 1353
Size: 2041 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4051 4425 4571 387
Size: 1064 138 2462 374 146 1280
Fragments arranged by size:
2462 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2368 2368
Size: 5464
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2333 2402 2774 3527 4124 4911 5179
Size: 393 701 69 372 753 597 787 268
Cuts at: 5179 1239
Size: 1524
Fragments arranged by size:
1524 787 753 701 597 393 372 268
69
EcoRI G'AATT_C
Cuts at: 2773 3151 2773
Size: 378 5086
FspI TGC'GCA
Cuts at: 3970 3970
Size: 5464
HaeII r_GCGC'y
Cuts at: 1678 2404 2439 4611 1678
Size: 726 35 2172 2531
Fragments arranged by size:
2531 2172 726 35
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2582 2614 4105 4137 4179
Size: 32 135 32 1010 32 1491 32 42
Cuts at: 4179 4211 1373
Size: 32 2626
Fragments arranged by size:
2626 1491 1010 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2808 3054 669
Size: 988 281 870 246 3079
Fragments arranged by size:
3079 988 870 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5464
HpaI GTT'AAC
Cuts at: 3054 3054
Size: 5464
KpnI G_GTAC'C
Cuts at: 2789 2789
Size: 5464
MfeI C'AATT_G
Cuts at: 2074 3063 2074
Size: 989 4475
MluI A'CGCG_T
Cuts at: 5340 5340
Size: 5464
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2243 2619 2625 4578 4762 1305
Size: 938 376 6 1953 184 2007
Fragments arranged by size:
2007 1953 938 376 184 6
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2180 10
Size: 54 1425 343 348 3294
Fragments arranged by size:
3294 1425 348 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2111 2195 2469 3422 3781 3940 518
Size: 648 945 84 274 953 359 159 2042
Fragments arranged by size:
2042 953 945 648 359 274 159 84
MspA1I CmG'CkG
Cuts at: 1606 2356 2449 2553 2880 3557 4705 4950 1606
Size: 750 93 104 327 677 1148 245 2120
Fragments arranged by size:
2120 1148 750 677 327 245 104 93
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3973
Fragments arranged by size:
3973 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5464
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5464
NotI GC'GGCC_GC
Cuts at: 2911 2911
Size: 5464
NspI r_CATG'y
Cuts at: 1132 1229 2037 2822 4367 1132
Size: 97 808 785 1545 2229
Fragments arranged by size:
2229 1545 808 785 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5464
PciI A'CATG_T
Cuts at: 2033 4363 2033
Size: 2330 3134
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5464
PfoI T'CCnGG_A
Cuts at: 2652 2652
Size: 5464
PmeI GTTT'AAAC
Cuts at: 2768 2768
Size: 5464
PmlI CAC'GTG
Cuts at: 2727 2727
Size: 5464
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3533 3565 5046 5078 3533
Size: 32 1481 32 3919
Fragments arranged by size:
3919 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2228 1613
Size: 615 4849
PsiI TTA'TAA
Cuts at: 3034 3034
Size: 5464
PstI C_TGCA'G
Cuts at: 830 2454 2816 830
Size: 1624 362 3478
Fragments arranged by size:
3478 1624 362
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5464
PvuII CAG'CTG
Cuts at: 2356 2449 2553 2356
Size: 93 104 5267
Fragments arranged by size:
5267 104 93
SacI G_AGCT'C
Cuts at: 2370 2370
Size: 5464
SalI G'TCGA_C
Cuts at: 1936 2806 1936
Size: 870 4594
SbfI CC_TGCA'GG
Cuts at: 2816 2816
Size: 5464
ScaI AGT'ACT
Cuts at: 3712 5424 3712
Size: 1712 3752
SfcI C'TryA_G
Cuts at: 826 1045 2450 2812 3947 4628 4819 826
Size: 219 1405 362 1135 681 191 1471
Fragments arranged by size:
1471 1405 1135 681 362 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5464
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5464
SmaI CCC'GGG
Cuts at: 1872 2791 1872
Size: 919 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2051 2135 2369 2747 3568 4469
Size: 197 926 108 84 234 378 821 901
Cuts at: 4469 4731 820
Size: 262 1553
Fragments arranged by size:
1553 926 901 821 378 262 234 197
108 84
SnaBI TAC'GTA
Cuts at: 493 3255 493
Size: 2762 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5464
SphI G_CATG'C
Cuts at: 1132 2822 1132
Size: 1690 3774
SspI AAT'ATT
Cuts at: 5 52 3388 5
Size: 47 3336 2081
Fragments arranged by size:
3336 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5464
StyI C'CwwG_G
Cuts at: 513 1065 2004 2903 513
Size: 552 939 899 3074
Fragments arranged by size:
3074 939 899 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 1997 3491 3508 3661 3846 4291 325
Size: 1255 417 1494 17 153 185 445 1498
Fragments arranged by size:
1498 1494 1255 445 417 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2280 2736 3710
Size: 274 80 33 51 1443 303 456 974
Cuts at: 3710 5422 96
Size: 1712 138
Fragments arranged by size:
1712 1443 974 456 303 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5424 5456 5424
Size: 32 5432
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5464
XbaI T'CTAG_A
Cuts at: 2800 2800
Size: 5464
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5464
XmaI C'CCGG_G
Cuts at: 1870 2789 1870
Size: 919 4545
XmnI GAAnn'nnTTC
Cuts at: 3593 3593
Size: 5464
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3272 276
Size: 53 83 186 936 1738 2468
Fragments arranged by size:
2468 1738 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI
PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoNI
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PpuMI PspOMI PspXI PsrI
RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI