Restriction map for
pFN21AE1378
(Circular) (Six-base) MAPSORT of: pFN21AE1378.seq Check: 9730 from: 1 to: 5452
pFN21AE1378 5452 bp
With 182 enzymes: SgfI *
August 16, 2010 14:46 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3262 278
Size: 53 83 186 936 1726 2468
Fragments arranged by size:
2468 1726 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2795 1937
Size: 858 4594
Acc65I G'GTAC_C
Cuts at: 2773 2773
Size: 5452
AclI AA'CG_TT
Cuts at: 1563 3579 3952 1563
Size: 2016 373 3063
Fragments arranged by size:
3063 2016 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3515 4899 5167 1632
Size: 1883 1384 268 1917
Fragments arranged by size:
1917 1883 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5255
AflIII A'CryG_T
Cuts at: 1203 2065 4351 5328 1203
Size: 862 2286 977 1327
Fragments arranged by size:
2286 1327 977 862
AgeI A'CCGG_T
Cuts at: 4283 4283
Size: 5452
AhdI GACnn_n'nnGTC
Cuts at: 4181 4181
Size: 5452
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5452
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5452
AlwNI CAG_nnn'CTG
Cuts at: 2237 3195 4767 2237
Size: 958 1572 2922
Fragments arranged by size:
2922 1572 958
ApaI G_GGCC'C
Cuts at: 2032 2032
Size: 5452
ApaLI G'TGCA_C
Cuts at: 2123 3509 4665 2123
Size: 1386 1156 2910
Fragments arranged by size:
2910 1386 1156
ApoI r'AATT_y
Cuts at: 1493 2074 2761 2987 3139 5248 1493
Size: 581 687 226 152 2109 1697
Fragments arranged by size:
2109 1697 687 581 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4006 160
Size: 872 2974 1606
Fragments arranged by size:
2974 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5452
AvaI C'yCGr_G
Cuts at: 1870 1943 2031 2777 1870
Size: 73 88 746 4545
Fragments arranged by size:
4545 746 88 73
BamHI G'GATC_C
Cuts at: 2782 3149 2782
Size: 367 5085
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2773 4228 618
Size: 325 217 627 986 1455 1842
Fragments arranged by size:
1842 1455 986 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2032 2628 2771 1391
Size: 641 596 143 4072
Fragments arranged by size:
4072 641 596 143
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2068 928
Size: 971 169 4312
Fragments arranged by size:
4312 971 169
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3643 3677 1903
Size: 34 1706 34 3678
Fragments arranged by size:
3678 1706 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3344 4554 3344
Size: 1210 4242
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5452
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2036 4063 136
Size: 107 122 71 631 605 364 2027 1525
Fragments arranged by size:
2027 1525 631 605 364 122 107 71
BglII A'GATC_T
Cuts at: 5447 5447
Size: 5452
BlpI GC'TnA_GC
Cuts at: 2869 2869
Size: 5452
Bme1580I G_kGCm'C
Cuts at: 1790 2032 2127 3513 4669 1790
Size: 242 95 1386 1156 2573
Fragments arranged by size:
2573 1386 1156 242 95
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5452
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2065 3183 4141
Size: 416 53 83 399 24 648 1118 958
Cuts at: 4141 442
Size: 1753
Fragments arranged by size:
1753 1118 958 648 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5452
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2127 2471 2709 4112 1239
Size: 888 344 238 1403 2579
Fragments arranged by size:
2579 1403 888 344 238
Bpu10I CC'TnA_GC
Cuts at: 2598 2598
Size: 5452
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2612 3577 4442 4740 2612
Size: 965 865 298 3324
Fragments arranged by size:
3324 965 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4115 882
Size: 617 2616 2219
Fragments arranged by size:
2616 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3243 493
Size: 711 2039 2702
Fragments arranged by size:
2702 2039 711
BsaBI GATnn'nnATC
Cuts at: 5446 5446
Size: 5452
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3259 3641 4276 275
Size: 53 83 186 936 1726 382 635 1451
Fragments arranged by size:
1726 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3885 4283 4557 4704 5115 3885
Size: 398 274 147 411 4222
Fragments arranged by size:
4222 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5227
Fragments arranged by size:
5227 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2124 1186
Size: 938 4514
BseYI C'CCAG_C
Cuts at: 4655 5151 4655
Size: 496 4956
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2392 1163
Size: 573 656 4223
Fragments arranged by size:
4223 656 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2902 3663 4691 1540
Size: 275 186 901 761 1028 2301
Fragments arranged by size:
2301 1028 901 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2127 2628 3513 3598 4669 2127
Size: 501 885 85 1071 2910
Fragments arranged by size:
2910 1071 885 501 85
BsmI GAATG_Cn'
Cuts at: 2963 3056 2963
Size: 93 5359
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2032 2127 2628 2771 3513 3598 4669
Size: 399 242 95 501 143 742 85 1071
Cuts at: 4669 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 501 399 242 143 95
85
BspEI T'CCGG_A
Cuts at: 5115 5115
Size: 5452
BspHI T'CATG_A
Cuts at: 3341 5071 3341
Size: 1730 3722
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2807 844
Size: 1963 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3339 1394
Size: 429 1516 3507
Fragments arranged by size:
3507 1516 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3947 4121 65
Size: 1183 2699 174 1396
Fragments arranged by size:
2699 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4096 4283 1700
Size: 110 2286 187 2869
Fragments arranged by size:
2869 2286 187 110
BsrGI T'GTAC_A
Cuts at: 96 2737 96
Size: 2641 2811
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5452
BssSI C'ACGA_G
Cuts at: 3512 4524 3512
Size: 1012 4440
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5452
BstBI TT'CG_AA
Cuts at: 2431 4256 2431
Size: 1825 3627
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5452
BstYI r'GATC_y
Cuts at: 1972 2782 3149 3534 3551 5003 5089 5101 5447
Size: 810 367 385 17 1452 86 12 346
Cuts at: 5447 1972
Size: 1977
Fragments arranged by size:
1977 1452 810 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2016 513
Size: 552 951 3949
Fragments arranged by size:
3949 951 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4713
Fragments arranged by size:
4713 683 56
BtsI GCAGTG_nn'
Cuts at: 2968 3762 3782 2968
Size: 794 20 4638
Fragments arranged by size:
4638 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5452
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5417
DraI TTT'AAA
Cuts at: 2756 2756
Size: 5452
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4459 809
Size: 507 3143 1802
Fragments arranged by size:
3143 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2899 3788 5417
Size: 54 1425 50 275 18 1069 889 1629
Cuts at: 5417 8
Size: 43
Fragments arranged by size:
1629 1425 1069 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2899 1537
Size: 275 1087 4090
Fragments arranged by size:
4090 1087 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3382 1353
Size: 2029 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4039 4413 4559 387
Size: 1064 138 2450 374 146 1280
Fragments arranged by size:
2450 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2626 2626
Size: 5452
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2127 2471 2709 3515 4112 4899 5167
Size: 393 495 344 238 806 597 787 268
Cuts at: 5167 1239
Size: 1524
Fragments arranged by size:
1524 806 787 597 495 393 344 268
238
EcoO109I rG'GnC_Cy
Cuts at: 2538 2723 2538
Size: 185 5267
EcoRI G'AATT_C
Cuts at: 2761 3139 2761
Size: 378 5074
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2765 2765
Size: 5452
FspI TGC'GCA
Cuts at: 3958 3958
Size: 5452
HaeII r_GCGC'y
Cuts at: 1678 2024 4599 1678
Size: 346 2575 2531
Fragments arranged by size:
2575 2531 346
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4093 4125 4167 4199 1373
Size: 32 135 32 2521 32 42 32 2626
Fragments arranged by size:
2626 2521 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2796 3042 669
Size: 988 281 858 246 3079
Fragments arranged by size:
3079 988 858 281 246
HindIII A'AGCT_T
Cuts at: 748 2468 748
Size: 1720 3732
HpaI GTT'AAC
Cuts at: 3042 3042
Size: 5452
KpnI G_GTAC'C
Cuts at: 2777 2777
Size: 5452
MfeI C'AATT_G
Cuts at: 2199 3051 2199
Size: 852 4600
MluI A'CGCG_T
Cuts at: 5328 5328
Size: 5452
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2365 4566 4750 1305
Size: 1060 2201 184 2007
Fragments arranged by size:
2201 2007 1060 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3630
Fragments arranged by size:
3630 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3410 3769 3928 518
Size: 648 2244 359 159 2042
Fragments arranged by size:
2244 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2018 2237 2687 2868 3545 4693 4938 1606
Size: 412 219 450 181 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 450 412 245 219 181
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4900
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5452
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5452
NotI GC'GGCC_GC
Cuts at: 2899 2899
Size: 5452
NspI r_CATG'y
Cuts at: 1132 1229 2069 2428 2810 4355 1132
Size: 97 840 359 382 1545 2229
Fragments arranged by size:
2229 1545 840 382 359 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5452
PciI A'CATG_T
Cuts at: 2065 4351 2065
Size: 2286 3166
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2663 1108
Size: 1555 3897
PfoI T'CCnGG_A
Cuts at: 2250 2250
Size: 5452
PmeI GTTT'AAAC
Cuts at: 2756 2756
Size: 5452
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3521 3553 5034 5066 3521
Size: 32 1481 32 3907
Fragments arranged by size:
3907 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2723 2723
Size: 5452
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5452
PsiI TTA'TAA
Cuts at: 3022 3022
Size: 5452
PspOMI G'GGCC_C
Cuts at: 2028 2028
Size: 5452
PstI C_TGCA'G
Cuts at: 830 2804 830
Size: 1974 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5452
PvuII CAG'CTG
Cuts at: 2237 2687 2237
Size: 450 5002
SacI G_AGCT'C
Cuts at: 2628 2628
Size: 5452
SacII CC_GC'GG
Cuts at: 2019 2019
Size: 5452
SalI G'TCGA_C
Cuts at: 1936 2794 1936
Size: 858 4594
SbfI CC_TGCA'GG
Cuts at: 2804 2804
Size: 5452
ScaI AGT'ACT
Cuts at: 3700 5412 3700
Size: 1712 3740
SfcI C'TryA_G
Cuts at: 826 1045 2800 3935 4616 4807 826
Size: 219 1755 1135 681 191 1471
Fragments arranged by size:
1755 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5452
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5452
SmaI CCC'GGG
Cuts at: 1872 2033 2779 1872
Size: 161 746 4545
Fragments arranged by size:
4545 746 161
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2627 3556 4457 4719 820
Size: 197 926 684 929 901 262 1553
Fragments arranged by size:
1553 929 926 901 684 262 197
SnaBI TAC'GTA
Cuts at: 493 3243 493
Size: 2750 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5452
SphI G_CATG'C
Cuts at: 1132 2428 2810 1132
Size: 1296 382 3774
Fragments arranged by size:
3774 1296 382
SrfI GCCC'GGGC
Cuts at: 2033 2033
Size: 5452
SspI AAT'ATT
Cuts at: 5 52 3376 5
Size: 47 3324 2081
Fragments arranged by size:
3324 2081 47
StuI AGG'CCT
Cuts at: 1517 2748 1517
Size: 1231 4221
StyI C'CwwG_G
Cuts at: 513 1065 2657 2718 2891 513
Size: 552 1592 61 173 3074
Fragments arranged by size:
3074 1592 552 173 61
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2265 2731 3479 3496 3649 3834 4279
Size: 1255 685 466 748 17 153 185 445
Cuts at: 4279 325
Size: 1498
Fragments arranged by size:
1498 1255 748 685 466 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2737 3698 5410
Size: 274 80 33 51 1443 760 961 1712
Cuts at: 5410 96
Size: 138
Fragments arranged by size:
1712 1443 961 760 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5412 5444 5412
Size: 32 5420
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5452
XbaI T'CTAG_A
Cuts at: 2788 2788
Size: 5452
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2270 2270
Size: 5452
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5452
XmaI C'CCGG_G
Cuts at: 1870 2031 2777 1870
Size: 161 746 4545
Fragments arranged by size:
4545 746 161
XmnI GAAnn'nnTTC
Cuts at: 3581 3581
Size: 5452
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3260 276
Size: 53 83 186 936 1726 2468
Fragments arranged by size:
2468 1726 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SacII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspXI PsrI RsrII SanDI
SapI SexAI SfiI SfoI SwaI