Restriction map for
pFN21AE1377
(Circular) (Six-base) MAPSORT of: pFN21AE1377.seq Check: 8121 from: 1 to: 6283
pFN21AE1377 6283 bp
With 182 enzymes: SgfI *
August 16, 2010 14:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4093 278
Size: 53 83 186 936 2557 2468
Fragments arranged by size:
2557 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3626 1937
Size: 1689 4594
Acc65I G'GTAC_C
Cuts at: 3604 3604
Size: 6283
AclI AA'CG_TT
Cuts at: 1563 4410 4783 1563
Size: 2847 373 3063
Fragments arranged by size:
3063 2847 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2074 2174 2187 2445 2698 3385 4346 5730
Size: 442 100 13 258 253 687 961 1384
Cuts at: 5730 5998 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 961 687 442 268 258 253
100 13
AflII C'TTAA_G
Cuts at: 820 1017 3371 820
Size: 197 2354 3732
Fragments arranged by size:
3732 2354 197
AflIII A'CryG_T
Cuts at: 1203 5182 6159 1203
Size: 3979 977 1327
Fragments arranged by size:
3979 1327 977
AgeI A'CCGG_T
Cuts at: 5114 5114
Size: 6283
AhdI GACnn_n'nnGTC
Cuts at: 3139 5012 3139
Size: 1873 4410
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6283
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6283
AlwNI CAG_nnn'CTG
Cuts at: 2031 4026 5598 2031
Size: 1995 1572 2716
Fragments arranged by size:
2716 1995 1572
ApaLI G'TGCA_C
Cuts at: 3071 4340 5496 3071
Size: 1269 1156 3858
Fragments arranged by size:
3858 1269 1156
ApoI r'AATT_y
Cuts at: 1493 3592 3818 3970 6079 1493
Size: 2099 226 152 2109 1697
Fragments arranged by size:
2109 2099 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4837 160
Size: 872 3805 1606
Fragments arranged by size:
3805 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6283
AvaI C'yCGr_G
Cuts at: 1870 1943 3107 3192 3608 1870
Size: 73 1164 85 416 4545
Fragments arranged by size:
4545 1164 416 85 73
AvrII C'CTAG_G
Cuts at: 2599 2599
Size: 6283
BamHI G'GATC_C
Cuts at: 2904 3613 3980 2904
Size: 709 367 5207
Fragments arranged by size:
5207 709 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3211 3604 5059 618
Size: 325 217 627 1424 393 1455 1842
Fragments arranged by size:
1842 1455 1424 627 393 325 217
BanII G_rGCy'C
Cuts at: 1391 2080 2346 2466 2722 2781 3114 3159 3602
Size: 689 266 120 256 59 333 45 443
Cuts at: 3602 1391
Size: 4072
Fragments arranged by size:
4072 689 443 333 266 256 120 59
45
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2992 3104 3231 3546 928
Size: 971 1093 112 127 315 3665
Fragments arranged by size:
3665 1093 971 315 127 112
BbvCI CC'TCA_GC
Cuts at: 2913 3366 2913
Size: 453 5830
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4474 4508 1903
Size: 34 2537 34 3678
Fragments arranged by size:
3678 2537 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4175 5385 4175
Size: 1210 5073
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6283
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4894 136
Size: 107 122 71 631 605 3222 1525
Fragments arranged by size:
3222 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2709 6278 2709
Size: 3569 2714
BlpI GC'TnA_GC
Cuts at: 2577 2679 3179 3700 2577
Size: 102 500 521 5160
Fragments arranged by size:
5160 521 500 102
Bme1580I G_kGCm'C
Cuts at: 1790 3075 3216 4344 5500 1790
Size: 1285 141 1128 1156 2573
Fragments arranged by size:
2573 1285 1156 1128 141
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6283
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2842 4014 4972
Size: 416 53 83 399 24 1425 1172 958
Cuts at: 4972 442
Size: 1753
Fragments arranged by size:
1753 1425 1172 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6283
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2258 2953 2258
Size: 695 5588
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2330 2765 3173 3452 4943 1239
Size: 1091 435 408 279 1491 2579
Fragments arranged by size:
2579 1491 1091 435 408 279
Bpu10I CC'TnA_GC
Cuts at: 2913 3366 2913
Size: 453 5830
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3050 4408 5273 5571 3050
Size: 1358 865 298 3762
Fragments arranged by size:
3762 1358 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4946 882
Size: 617 3447 2219
Fragments arranged by size:
3447 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4074 493
Size: 711 2870 2702
Fragments arranged by size:
2870 2702 711
BsaBI GATnn'nnATC
Cuts at: 6277 6277
Size: 6283
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4090 4472 5107 275
Size: 53 83 186 936 2557 382 635 1451
Fragments arranged by size:
2557 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4716 5114 5388 5535 5946 4716
Size: 398 274 147 411 5053
Fragments arranged by size:
5053 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2096 2126 3141 3171 1178
Size: 30 165 30 693 30 1015 30 4290
Fragments arranged by size:
4290 1015 693 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2952 1186
Size: 1766 4517
BseYI C'CCAG_C
Cuts at: 2956 2991 5486 5982 2956
Size: 35 2495 496 3257
Fragments arranged by size:
3257 2495 496 35
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5710
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2616 3733 4494 5522 1540
Size: 275 186 615 1117 761 1028 2301
Fragments arranged by size:
2301 1117 1028 761 615 275 186
BsiHKAI G_wGCw'C
Cuts at: 2781 3075 3159 4344 4429 5500 2781
Size: 294 84 1185 85 1071 3564
Fragments arranged by size:
3564 1185 1071 294 85 84
BsmI GAATG_Cn'
Cuts at: 3794 3887 3794
Size: 93 6190
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2080 2346 2466 2722 2781 3075 3114
Size: 399 290 266 120 256 59 294 39
Cuts at: 3114 3159 3216 3602 4344 4429 5500 1391
Size: 45 57 386 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 386 294 290 266
256 120 85 59 57 45 39
BspEI T'CCGG_A
Cuts at: 5946 5946
Size: 6283
BspHI T'CATG_A
Cuts at: 3093 4172 5902 3093
Size: 1079 1730 3474
Fragments arranged by size:
3474 1730 1079
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3638 844
Size: 2794 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4170 1394
Size: 429 2347 3507
Fragments arranged by size:
3507 2347 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2382 2830 3208 4778 4952 65
Size: 1183 1134 448 378 1570 174 1396
Fragments arranged by size:
1570 1396 1183 1134 448 378 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4927 5114 1700
Size: 110 3117 187 2869
Fragments arranged by size:
3117 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2142 96
Size: 2046 4237
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6283
BssSI C'ACGA_G
Cuts at: 2446 4343 5355 2446
Size: 1897 1012 3374
Fragments arranged by size:
3374 1897 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6283
BstBI TT'CG_AA
Cuts at: 5087 5087
Size: 6283
BstEII G'GTnAC_C
Cuts at: 1184 3296 1184
Size: 2112 4171
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 6283
BstYI r'GATC_y
Cuts at: 1972 2334 2709 2904 3613 3980 4365 4382 5834
Size: 362 375 195 709 367 385 17 1452
Cuts at: 5834 5920 5932 6278 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 709 385 375 367 362 346
195 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3027 3027
Size: 6283
BtgI C'CryG_G
Cuts at: 513 1065 2838 513
Size: 552 1773 3958
Fragments arranged by size:
3958 1773 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2504 487
Size: 683 56 1278 4266
Fragments arranged by size:
4266 1278 683 56
BtsI GCAGTG_nn'
Cuts at: 2223 3799 4593 4613 2223
Size: 1576 794 20 3893
Fragments arranged by size:
3893 1576 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6283
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2598 2633 529
Size: 35 2034 35 4179
Fragments arranged by size:
4179 2034 35 35
DraI TTT'AAA
Cuts at: 3587 3587
Size: 6283
DraIII CAC_nnn'GTG
Cuts at: 2743 2743
Size: 6283
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5290 809
Size: 507 3974 1802
Fragments arranged by size:
3974 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2442 2583 3730
Size: 54 1425 50 275 18 612 141 1147
Cuts at: 3730 4619 6248 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1147 889 612 275 141 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3730 1537
Size: 275 1918 4090
Fragments arranged by size:
4090 1918 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2769 3015 3404 4213 1353
Size: 1416 246 389 809 3423
Fragments arranged by size:
3423 1416 809 389 246
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4870 5244 5390 387
Size: 1064 138 3281 374 146 1280
Fragments arranged by size:
3281 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2779 3157 2779
Size: 378 5905
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2074 2174 2187 2330 2445 2698 2765
Size: 393 442 100 13 143 115 253 67
Cuts at: 2765 3173 3385 3452 4346 4943 5730 5998 1239
Size: 408 212 67 894 597 787 268 1524
Fragments arranged by size:
1524 894 787 597 442 408 393 268
253 212 143 115 100 67 67 13
EcoNI CCTnn'n_nnAGG
Cuts at: 2015 2603 2015
Size: 588 5695
EcoO109I rG'GnC_Cy
Cuts at: 2104 3427 2104
Size: 1323 4960
EcoRI G'AATT_C
Cuts at: 3592 3970 3592
Size: 378 5905
FspI TGC'GCA
Cuts at: 3547 4789 3547
Size: 1242 5041
HaeII r_GCGC'y
Cuts at: 1678 5430 1678
Size: 3752 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2096 2128 2630 2662 2896
Size: 32 135 32 524 32 502 32 234
Cuts at: 2896 2928 3125 3157 4924 4956 4998 5030 1373
Size: 32 197 32 1767 32 42 32 2626
Fragments arranged by size:
2626 1767 524 502 234 197 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3627 3873 669
Size: 988 281 1689 246 3079
Fragments arranged by size:
3079 1689 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6283
HpaI GTT'AAC
Cuts at: 3873 3873
Size: 6283
KpnI G_GTAC'C
Cuts at: 3608 3608
Size: 6283
MfeI C'AATT_G
Cuts at: 3882 3882
Size: 6283
MluI A'CGCG_T
Cuts at: 6159 6159
Size: 6283
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2254 2973 5397 5581 1305
Size: 949 719 2424 184 2007
Fragments arranged by size:
2424 2007 949 719 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2444 2585 10
Size: 54 1425 343 612 141 3708
Fragments arranged by size:
3708 1425 612 343 141 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2450 2843 4241 4600 4759 518
Size: 648 1284 393 1398 359 159 2042
Fragments arranged by size:
2042 1398 1284 648 393 359 159
MspA1I CmG'CkG
Cuts at: 1606 2054 2320 2581 2678 2995 3699 4376 5524
Size: 448 266 261 97 317 704 677 1148
Cuts at: 5524 5769 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 704 677 448 317 266 261
245 97
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5731
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6283
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6283
NotI GC'GGCC_GC
Cuts at: 3730 3730
Size: 6283
NspI r_CATG'y
Cuts at: 1132 1229 2143 3641 5186 1132
Size: 97 914 1498 1545 2229
Fragments arranged by size:
2229 1545 1498 914 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6283
PciI A'CATG_T
Cuts at: 5182 5182
Size: 6283
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6283
PfoI T'CCnGG_A
Cuts at: 2100 2114 3129 2100
Size: 14 1015 5254
Fragments arranged by size:
5254 1015 14
PmeI GTTT'AAAC
Cuts at: 3587 3587
Size: 6283
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4352 4384 5865 5897 4352
Size: 32 1481 32 4738
Fragments arranged by size:
4738 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2104 3427 2104
Size: 1323 4960
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6283
PsiI TTA'TAA
Cuts at: 3853 3853
Size: 6283
PstI C_TGCA'G
Cuts at: 830 3635 830
Size: 2805 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6283
PvuII CAG'CTG
Cuts at: 2320 2581 2678 2995 2320
Size: 261 97 317 5608
Fragments arranged by size:
5608 317 261 97
SacI G_AGCT'C
Cuts at: 2781 3159 2781
Size: 378 5905
SalI G'TCGA_C
Cuts at: 1936 3625 1936
Size: 1689 4594
SapI GCTCTTCn'nnn_
Cuts at: 2769 3015 2769
Size: 246 6037
SbfI CC_TGCA'GG
Cuts at: 3635 3635
Size: 6283
ScaI AGT'ACT
Cuts at: 4531 6243 4531
Size: 1712 4571
SfcI C'TryA_G
Cuts at: 826 1045 3118 3552 3631 4766 5447 5638 826
Size: 219 2073 434 79 1135 681 191 1471
Fragments arranged by size:
2073 1471 1135 681 434 219 191 79
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6283
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6283
SmaI CCC'GGG
Cuts at: 1872 3109 3610 1872
Size: 1237 501 4545
Fragments arranged by size:
4545 1237 501
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3065 3371 4387 5288 5550 820
Size: 197 926 1122 306 1016 901 262 1553
Fragments arranged by size:
1553 1122 1016 926 901 306 262 197
SnaBI TAC'GTA
Cuts at: 493 4074 493
Size: 3581 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6283
SphI G_CATG'C
Cuts at: 1132 3641 1132
Size: 2509 3774
SrfI GCCC'GGGC
Cuts at: 3109 3109
Size: 6283
SspI AAT'ATT
Cuts at: 5 52 4207 5
Size: 47 4155 2081
Fragments arranged by size:
4155 2081 47
StuI AGG'CCT
Cuts at: 1517 2180 1517
Size: 663 5620
StyI C'CwwG_G
Cuts at: 513 1065 2599 3301 3321 3722 513
Size: 552 1534 702 20 401 3074
Fragments arranged by size:
3074 1534 702 552 401 20
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2704 2710 3284 4310 4327 4480 4665
Size: 1255 1124 6 574 1026 17 153 185
Cuts at: 4665 5110 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1124 1026 574 445 185 153
17 6
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2142 3081 4529
Size: 274 80 33 51 1443 165 939 1448
Cuts at: 4529 6241 96
Size: 1712 138
Fragments arranged by size:
1712 1448 1443 939 274 165 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3482 3514 6243 6275 3482
Size: 32 2729 32 3490
Fragments arranged by size:
3490 2729 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6283
XbaI T'CTAG_A
Cuts at: 3619 3619
Size: 6283
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 6283
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6283
XmaI C'CCGG_G
Cuts at: 1870 3107 3608 1870
Size: 1237 501 4545
Fragments arranged by size:
4545 1237 501
XmnI GAAnn'nnTTC
Cuts at: 4412 4412
Size: 6283
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4091 276
Size: 53 83 186 936 2557 2468
Fragments arranged by size:
2557 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BfrBI
BsiWI BsmBI BstZ17I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI
PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI
SwaI