Restriction map for
pFN21AE1375
(Circular) (Six-base) MAPSORT of: pFN21AE1375.seq Check: 9320 from: 1 to: 5341
pFN21AE1375 5341 bp
With 182 enzymes: SgfI *
August 16, 2010 14:46 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3151 278
Size: 53 83 186 936 1615 2468
Fragments arranged by size:
2468 1615 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2684 1937
Size: 747 4594
Acc65I G'GTAC_C
Cuts at: 2662 2662
Size: 5341
AclI AA'CG_TT
Cuts at: 1563 3468 3841 1563
Size: 1905 373 3063
Fragments arranged by size:
3063 1905 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2603 3404 4788 5056 1632
Size: 971 801 1384 268 1917
Fragments arranged by size:
1917 1384 971 801 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5144
AflIII A'CryG_T
Cuts at: 1203 4240 5217 1203
Size: 3037 977 1327
Fragments arranged by size:
3037 1327 977
AgeI A'CCGG_T
Cuts at: 4172 4172
Size: 5341
AhdI GACnn_n'nnGTC
Cuts at: 4070 4070
Size: 5341
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5341
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5341
AlwNI CAG_nnn'CTG
Cuts at: 2134 3084 4656 2134
Size: 950 1572 2819
Fragments arranged by size:
2819 1572 950
ApaLI G'TGCA_C
Cuts at: 3398 4554 3398
Size: 1156 4185
ApoI r'AATT_y
Cuts at: 1493 2199 2300 2340 2501 2650 2876 3028 5137
Size: 706 101 40 161 149 226 152 2109
Cuts at: 5137 1493
Size: 1697
Fragments arranged by size:
2109 1697 706 226 161 152 149 101
40
AseI AT'TA_AT
Cuts at: 160 1032 3895 160
Size: 872 2863 1606
Fragments arranged by size:
2863 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5341
AvaI C'yCGr_G
Cuts at: 1870 1943 2666 1870
Size: 73 723 4545
Fragments arranged by size:
4545 723 73
BamHI G'GATC_C
Cuts at: 2671 3038 2671
Size: 367 4974
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2662 4117 618
Size: 325 217 627 875 1455 1842
Fragments arranged by size:
1842 1455 875 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2660 1391
Size: 1269 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2440 928
Size: 971 541 3829
Fragments arranged by size:
3829 971 541
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3532 3566 1903
Size: 34 1595 34 3678
Fragments arranged by size:
3678 1595 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2094 3233 4443 2094
Size: 1139 1210 2992
Fragments arranged by size:
2992 1210 1139
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5341
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3952 136
Size: 107 122 71 631 605 2280 1525
Fragments arranged by size:
2280 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5336 5336
Size: 5341
BlpI GC'TnA_GC
Cuts at: 2758 2758
Size: 5341
Bme1580I G_kGCm'C
Cuts at: 1790 2482 3402 4558 1790
Size: 692 920 1156 2573
Fragments arranged by size:
2573 1156 920 692
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5341
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3072 4030 442
Size: 416 53 83 399 24 1655 958 1753
Fragments arranged by size:
1753 1655 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5341
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4001 1239
Size: 2762 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3466 4331 4629 3466
Size: 865 298 4178
Fragments arranged by size:
4178 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2036 2180 4004 882
Size: 617 537 144 1824 2219
Fragments arranged by size:
2219 1824 617 537 144
BsaAI yAC'GTr
Cuts at: 493 1204 3132 493
Size: 711 1928 2702
Fragments arranged by size:
2702 1928 711
BsaBI GATnn'nnATC
Cuts at: 5335 5335
Size: 5341
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3148 3530 4165 275
Size: 53 83 186 936 1615 382 635 1451
Fragments arranged by size:
1615 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3774 4172 4446 4593 5004 3774
Size: 398 274 147 411 4111
Fragments arranged by size:
4111 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5116
Fragments arranged by size:
5116 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5341
BseYI C'CCAG_C
Cuts at: 4544 5040 4544
Size: 496 4845
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4768
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2791 3552 4580 1540
Size: 275 186 790 761 1028 2301
Fragments arranged by size:
2301 1028 790 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2332 3402 3487 4558 2332
Size: 1070 85 1071 3115
Fragments arranged by size:
3115 1071 1070 85
BsmI GAATG_Cn'
Cuts at: 2852 2945 2852
Size: 93 5248
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2332 2482 2660 3402 3487 4558 1391
Size: 399 542 150 178 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 542 399 178 150 85
BspEI T'CCGG_A
Cuts at: 5004 5004
Size: 5341
BspHI T'CATG_A
Cuts at: 3230 4960 3230
Size: 1730 3611
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2696 844
Size: 1852 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3228 1394
Size: 429 1405 3507
Fragments arranged by size:
3507 1405 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3836 4010 65
Size: 1183 2588 174 1396
Fragments arranged by size:
2588 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3985 4172 1700
Size: 110 2175 187 2869
Fragments arranged by size:
2869 2175 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5341
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5341
BssSI C'ACGA_G
Cuts at: 2325 3401 4413 2325
Size: 1076 1012 3253
Fragments arranged by size:
3253 1076 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5341
BstBI TT'CG_AA
Cuts at: 4145 4145
Size: 5341
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5341
BstXI CCAn_nnnn'nTGG
Cuts at: 2053 2053
Size: 5341
BstYI r'GATC_y
Cuts at: 1972 2352 2671 3038 3423 3440 4892 4978 4990
Size: 380 319 367 385 17 1452 86 12
Cuts at: 4990 5336 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 385 380 367 346 319 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2052 513
Size: 552 987 3802
Fragments arranged by size:
3802 987 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4602
Fragments arranged by size:
4602 683 56
BtsI GCAGTG_nn'
Cuts at: 2857 3651 3671 2857
Size: 794 20 4527
Fragments arranged by size:
4527 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5341
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5306
DraI TTT'AAA
Cuts at: 2551 2645 2551
Size: 94 5247
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4348 809
Size: 507 3032 1802
Fragments arranged by size:
3032 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2637 2788 3677
Size: 54 1425 50 275 18 807 151 889
Cuts at: 3677 5306 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 807 275 151 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2788 1537
Size: 275 976 4090
Fragments arranged by size:
4090 976 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2016 2265 3271 1353
Size: 663 249 1006 3423
Fragments arranged by size:
3423 1006 663 249
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3928 4302 4448 387
Size: 1064 138 2339 374 146 1280
Fragments arranged by size:
2339 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2603 3404 4001 4788 5056 1239
Size: 393 971 801 597 787 268 1524
Fragments arranged by size:
1524 971 801 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2300 2650 3028 2300
Size: 350 378 4613
Fragments arranged by size:
4613 378 350
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2444 2444
Size: 5341
FspI TGC'GCA
Cuts at: 3847 3847
Size: 5341
HaeII r_GCGC'y
Cuts at: 1678 4488 1678
Size: 2810 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2033 2065 3982 4014 4056
Size: 32 135 32 461 32 1917 32 42
Cuts at: 4056 4088 1373
Size: 32 2626
Fragments arranged by size:
2626 1917 461 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2227 2685 2931 669
Size: 988 281 289 458 246 3079
Fragments arranged by size:
3079 988 458 289 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5341
HpaI GTT'AAC
Cuts at: 2227 2931 2227
Size: 704 4637
KpnI G_GTAC'C
Cuts at: 2666 2666
Size: 5341
MfeI C'AATT_G
Cuts at: 2940 2940
Size: 5341
MluI A'CGCG_T
Cuts at: 5217 5217
Size: 5341
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4455 4639 1305
Size: 3150 184 2007
Fragments arranged by size:
3150 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3519
Fragments arranged by size:
3519 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2051 2192 3299 3658 3817 518
Size: 648 843 42 141 1107 359 159 2042
Fragments arranged by size:
2042 1107 843 648 359 159 141 42
MspA1I CmG'CkG
Cuts at: 1606 2258 2757 3434 4582 4827 1606
Size: 652 499 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 652 499 245
NcoI C'CATG_G
Cuts at: 513 1065 2052 513
Size: 552 987 3802
Fragments arranged by size:
3802 987 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5341
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5341
NotI GC'GGCC_GC
Cuts at: 2788 2788
Size: 5341
NspI r_CATG'y
Cuts at: 1132 1229 2699 4244 1132
Size: 97 1470 1545 2229
Fragments arranged by size:
2229 1545 1470 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5341
PciI A'CATG_T
Cuts at: 4240 4240
Size: 5341
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5341
PmeI GTTT'AAAC
Cuts at: 2645 2645
Size: 5341
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3410 3442 4923 4955 3410
Size: 32 1481 32 3796
Fragments arranged by size:
3796 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5341
PsiI TTA'TAA
Cuts at: 2911 2911
Size: 5341
PstI C_TGCA'G
Cuts at: 830 2125 2391 2693 830
Size: 1295 266 302 3478
Fragments arranged by size:
3478 1295 302 266
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5341
PvuII CAG'CTG
Cuts at: 2258 2258
Size: 5341
SalI G'TCGA_C
Cuts at: 1936 2683 1936
Size: 747 4594
SbfI CC_TGCA'GG
Cuts at: 2693 2693
Size: 5341
ScaI AGT'ACT
Cuts at: 2513 3589 5301 2513
Size: 1076 1712 2553
Fragments arranged by size:
2553 1712 1076
SfcI C'TryA_G
Cuts at: 826 1045 2121 2387 2689 3824 4505 4696 826
Size: 219 1076 266 302 1135 681 191 1471
Fragments arranged by size:
1471 1135 1076 681 302 266 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5341
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5341
SmaI CCC'GGG
Cuts at: 1872 2668 1872
Size: 796 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3445 4346 4608 820
Size: 197 926 1502 901 262 1553
Fragments arranged by size:
1553 1502 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3132 493
Size: 2639 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5341
SphI G_CATG'C
Cuts at: 1132 2699 1132
Size: 1567 3774
SspI AAT'ATT
Cuts at: 5 52 3265 5
Size: 47 3213 2081
Fragments arranged by size:
3213 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5341
StyI C'CwwG_G
Cuts at: 513 1065 2052 2780 513
Size: 552 987 728 3074
Fragments arranged by size:
3074 987 728 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3368 3385 3538 3723 4168 325
Size: 1255 1788 17 153 185 445 1498
Fragments arranged by size:
1788 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2511 3587 5299
Size: 274 80 33 51 1443 534 1076 1712
Cuts at: 5299 96
Size: 138
Fragments arranged by size:
1712 1443 1076 534 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5301 5333 5301
Size: 32 5309
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5341
XbaI T'CTAG_A
Cuts at: 2677 2677
Size: 5341
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5341
XmaI C'CCGG_G
Cuts at: 1870 2666 1870
Size: 796 4545
XmnI GAAnn'nnTTC
Cuts at: 3470 3470
Size: 5341
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3149 276
Size: 53 83 186 936 1615 2468
Fragments arranged by size:
2468 1615 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI