Restriction map for
pFN21AE1372
(Circular) (Six-base) MAPSORT of: pFN21AE1372.seq Check: 9027 from: 1 to: 6439
pFN21AE1372 6439 bp
With 182 enzymes: SgfI *
August 16, 2010 14:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4249 278
Size: 53 83 186 936 2713 2468
Fragments arranged by size:
2713 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3782 1937
Size: 1845 4594
Acc65I G'GTAC_C
Cuts at: 3760 3760
Size: 6439
AclI AA'CG_TT
Cuts at: 1563 4566 4939 1563
Size: 3003 373 3063
Fragments arranged by size:
3063 3003 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2470 2557 2948 3015 3384 4502 5886 6154
Size: 838 87 391 67 369 1118 1384 268
Cuts at: 6154 1632
Size: 1917
Fragments arranged by size:
1917 1384 1118 838 391 369 268 87
67
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6242
AflIII A'CryG_T
Cuts at: 1203 5338 6315 1203
Size: 4135 977 1327
Fragments arranged by size:
4135 1327 977
AgeI A'CCGG_T
Cuts at: 5270 5270
Size: 6439
AhdI GACnn_n'nnGTC
Cuts at: 5168 5168
Size: 6439
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6439
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6439
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2204 2236 2204
Size: 32 6407
AlwNI CAG_nnn'CTG
Cuts at: 2239 3323 4182 5754 2239
Size: 1084 859 1572 2924
Fragments arranged by size:
2924 1572 1084 859
ApaI G_GGCC'C
Cuts at: 3159 3538 3159
Size: 379 6060
ApaLI G'TGCA_C
Cuts at: 4496 5652 4496
Size: 1156 5283
ApoI r'AATT_y
Cuts at: 1493 2114 3431 3748 3974 4126 6235 1493
Size: 621 1317 317 226 152 2109 1697
Fragments arranged by size:
2109 1697 1317 621 317 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4993 160
Size: 872 3961 1606
Fragments arranged by size:
3961 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6439
AvaI C'yCGr_G
Cuts at: 1870 1943 2423 3764 1870
Size: 73 480 1341 4545
Fragments arranged by size:
4545 1341 480 73
BamHI G'GATC_C
Cuts at: 3502 3769 4136 3502
Size: 267 367 5805
Fragments arranged by size:
5805 367 267
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2524 2787 3298 3760 5215
Size: 325 217 627 737 263 511 462 1455
Cuts at: 5215 618
Size: 1842
Fragments arranged by size:
1842 1455 737 627 511 462 325 263
217
BanII G_rGCy'C
Cuts at: 1391 2752 3003 3063 3135 3146 3159 3538 3758
Size: 1361 251 60 72 11 13 379 220
Cuts at: 3758 1391
Size: 4072
Fragments arranged by size:
4072 1361 379 251 220 72 60 13
11
BbeI G_GCGC'C
Cuts at: 2791 2791
Size: 6439
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3318 928
Size: 971 1419 4049
Fragments arranged by size:
4049 1419 971
BbvCI CC'TCA_GC
Cuts at: 2042 2042
Size: 6439
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4630 4664 1903
Size: 34 50 34 2609 34 3678
Fragments arranged by size:
3678 2609 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4331 5541 4331
Size: 1210 5229
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6439
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5050 136
Size: 107 122 71 631 605 3378 1525
Fragments arranged by size:
3378 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6434 6434
Size: 6439
BlpI GC'TnA_GC
Cuts at: 3856 3856
Size: 6439
Bme1580I G_kGCm'C
Cuts at: 1790 3159 3538 4500 5656 1790
Size: 1369 379 962 1156 2573
Fragments arranged by size:
2573 1369 1156 962 379
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6439
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2799 2998 4170
Size: 416 53 83 399 24 1382 199 1172
Cuts at: 4170 5128 442
Size: 958 1753
Fragments arranged by size:
1753 1382 1172 958 416 399 199 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6439
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2692 2692
Size: 6439
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2610 2756 3413 3640 5099 1239
Size: 1371 146 657 227 1459 2579
Fragments arranged by size:
2579 1459 1371 657 227 146
Bpu10I CC'TnA_GC
Cuts at: 2042 3140 2042
Size: 1098 5341
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3607 4564 5429 5727 3607
Size: 957 865 298 4319
Fragments arranged by size:
4319 957 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2861 3677 5102 882
Size: 617 1362 816 1425 2219
Fragments arranged by size:
2219 1425 1362 816 617
BsaAI yAC'GTr
Cuts at: 493 1204 4230 493
Size: 711 3026 2702
Fragments arranged by size:
3026 2702 711
BsaBI GATnn'nnATC
Cuts at: 6433 6433
Size: 6439
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2788 4246 4628 5263
Size: 53 83 186 936 1255 1458 382 635
Cuts at: 5263 275
Size: 1451
Fragments arranged by size:
1458 1451 1255 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2744 4872 5270 5544 5691 6102 2744
Size: 2128 398 274 147 411 3081
Fragments arranged by size:
3081 2128 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2204 2234 1178
Size: 30 165 30 801 30 5383
Fragments arranged by size:
5383 801 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2030 2681 2912 3002 1186
Size: 844 651 231 90 4623
Fragments arranged by size:
4623 844 651 231 90
BseYI C'CCAG_C
Cuts at: 2084 2828 3046 3070 3134 3146 3242 3316 5642
Size: 744 218 24 64 12 96 74 2326
Cuts at: 5642 6138 2084
Size: 496 2385
Fragments arranged by size:
2385 2326 744 496 218 96 74 64
24 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2927 1163
Size: 573 1191 4675
Fragments arranged by size:
4675 1191 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3889 4650 5678 1540
Size: 275 186 1888 761 1028 2301
Fragments arranged by size:
2301 1888 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4500 4585 5656 4500
Size: 85 1071 5283
Fragments arranged by size:
5283 1071 85
BsmI GAATG_Cn'
Cuts at: 3329 3950 4043 3329
Size: 621 93 5725
Fragments arranged by size:
5725 621 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2752 3003 3063 3135 3146 3159 3538
Size: 399 962 251 60 72 11 13 379
Cuts at: 3538 3758 4500 4585 5656 1391
Size: 220 742 85 1071 2174
Fragments arranged by size:
2174 1071 962 742 399 379 251 220
85 72 60 13 11
BspEI T'CCGG_A
Cuts at: 2744 6102 2744
Size: 3358 3081
BspHI T'CATG_A
Cuts at: 4328 6058 4328
Size: 1730 4709
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2177 2949 3794 844
Size: 1333 772 845 3489
Fragments arranged by size:
3489 1333 845 772
BsrBI CCG'CTC
Cuts at: 1394 1823 4326 1394
Size: 429 2503 3507
Fragments arranged by size:
3507 2503 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2290 4934 5108 65
Size: 1183 1042 2644 174 1396
Fragments arranged by size:
2644 1396 1183 1042 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2322 5083 5270 1700
Size: 110 512 2761 187 2869
Fragments arranged by size:
2869 2761 512 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6439
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6439
BssSI C'ACGA_G
Cuts at: 4499 5511 4499
Size: 1012 5427
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6439
BstBI TT'CG_AA
Cuts at: 5243 5243
Size: 6439
BstEII G'GTnAC_C
Cuts at: 1184 3338 3700 1184
Size: 2154 362 3923
Fragments arranged by size:
3923 2154 362
BstXI CCAn_nnnn'nTGG
Cuts at: 2285 2285
Size: 6439
BstYI r'GATC_y
Cuts at: 1972 2403 3117 3502 3769 4136 4521 4538 5990
Size: 431 714 385 267 367 385 17 1452
Cuts at: 5990 6076 6088 6434 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 714 431 385 385 367 346
267 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2189 3294 3344 3529 2189
Size: 1105 50 185 5099
Fragments arranged by size:
5099 1105 185 50
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5887
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5700
Fragments arranged by size:
5700 683 56
BtsI GCAGTG_nn'
Cuts at: 2265 2395 3604 3955 4749 4769 2265
Size: 130 1209 351 794 20 3935
Fragments arranged by size:
3935 1209 794 351 130 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6439
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6404
DraI TTT'AAA
Cuts at: 3743 3743
Size: 6439
DraIII CAC_nnn'GTG
Cuts at: 3257 3257
Size: 6439
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5446 809
Size: 507 4130 1802
Fragments arranged by size:
4130 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3480 3886 4775
Size: 54 1425 50 275 18 1650 406 889
Cuts at: 4775 6404 8
Size: 1629 43
Fragments arranged by size:
1650 1629 1425 889 406 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3886 1537
Size: 275 2074 4090
Fragments arranged by size:
4090 2074 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2822 2991 3360 4369 1353
Size: 1469 169 369 1009 3423
Fragments arranged by size:
3423 1469 1009 369 169
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5026 5400 5546 387
Size: 1064 138 3437 374 146 1280
Fragments arranged by size:
3437 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2470 2557 2610 2756 2948 3015 3384
Size: 393 838 87 53 146 192 67 369
Cuts at: 3384 3413 3640 4502 5099 5886 6154 1239
Size: 29 227 862 597 787 268 1524
Fragments arranged by size:
1524 862 838 787 597 393 369 268
227 192 146 87 67 53 29
EcoNI CCTnn'n_nnAGG
Cuts at: 3482 3482
Size: 6439
EcoO109I rG'GnC_Cy
Cuts at: 3155 3534 3535 3155
Size: 379 1 6059
Fragments arranged by size:
6059 379 1
EcoRI G'AATT_C
Cuts at: 3748 4126 3748
Size: 378 6061
FspI TGC'GCA
Cuts at: 4945 4945
Size: 6439
HaeII r_GCGC'y
Cuts at: 1678 2039 2791 5586 1678
Size: 361 752 2795 2531
Fragments arranged by size:
2795 2531 752 361
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3386 3418 3557 3589 5080
Size: 32 135 32 1814 32 139 32 1491
Cuts at: 5080 5112 5154 5186 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1814 1491 139 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3783 4029 669
Size: 988 281 1845 246 3079
Fragments arranged by size:
3079 1845 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6439
HpaI GTT'AAC
Cuts at: 4029 4029
Size: 6439
KasI G'GCGC_C
Cuts at: 2787 2787
Size: 6439
KpnI G_GTAC'C
Cuts at: 3764 3764
Size: 6439
MfeI C'AATT_G
Cuts at: 4038 4038
Size: 6439
MluI A'CGCG_T
Cuts at: 6315 6315
Size: 6439
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2247 2355 5553 5737 1305
Size: 942 108 3198 184 2007
Fragments arranged by size:
3198 2007 942 184 108
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3482 10
Size: 54 1425 343 1650 2967
Fragments arranged by size:
2967 1650 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2283 4397 4756 4915 518
Size: 648 1117 2114 359 159 2042
Fragments arranged by size:
2114 2042 1117 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2776 3081 3114 3320 3594 3855 4532 5680
Size: 1170 305 33 206 274 261 677 1148
Cuts at: 5680 5925 1606
Size: 245 2120
Fragments arranged by size:
2120 1170 1148 677 305 274 261 245
206 33
NaeI GCC'GGC
Cuts at: 2324 2324
Size: 6439
NarI GG'CG_CC
Cuts at: 2788 2788
Size: 6439
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5887
NdeI CA'TA_TG
Cuts at: 387 2473 387
Size: 2086 4353
NgoMIV G'CCGG_C
Cuts at: 2322 2322
Size: 6439
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6439
NotI GC'GGCC_GC
Cuts at: 3886 3886
Size: 6439
NspI r_CATG'y
Cuts at: 1132 1229 3797 5342 1132
Size: 97 2568 1545 2229
Fragments arranged by size:
2568 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6439
PciI A'CATG_T
Cuts at: 5338 5338
Size: 6439
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6439
PfoI T'CCnGG_A
Cuts at: 2207 2207
Size: 6439
PmeI GTTT'AAAC
Cuts at: 3743 3743
Size: 6439
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2204 2236 2990 3022 4508 4540 6021 6053 2204
Size: 32 754 32 1486 32 1481 32 2590
Fragments arranged by size:
2590 1486 1481 754 32 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2708 1613
Size: 1095 5344
PsiI TTA'TAA
Cuts at: 4009 4009
Size: 6439
PspOMI G'GGCC_C
Cuts at: 3155 3534 3155
Size: 379 6060
PstI C_TGCA'G
Cuts at: 830 2991 3086 3129 3599 3729 3791 830
Size: 2161 95 43 470 130 62 3478
Fragments arranged by size:
3478 2161 470 130 95 62 43
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6439
PvuII CAG'CTG
Cuts at: 2776 3081 3320 3594 2776
Size: 305 239 274 5621
Fragments arranged by size:
5621 305 274 239
SalI G'TCGA_C
Cuts at: 1936 3781 1936
Size: 1845 4594
SapI GCTCTTCn'nnn_
Cuts at: 2822 2991 2822
Size: 169 6270
SbfI CC_TGCA'GG
Cuts at: 3129 3791 3129
Size: 662 5777
ScaI AGT'ACT
Cuts at: 4687 6399 4687
Size: 1712 4727
SexAI A'CCwGG_T
Cuts at: 3696 3696
Size: 6439
SfcI C'TryA_G
Cuts at: 826 1045 2227 2987 3082 3125 3595 3725 3787
Size: 219 1182 760 95 43 470 130 62
Cuts at: 3787 4922 5603 5794 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1182 1135 760 681 470 219 191
130 95 62 43
SfoI GGC'GCC
Cuts at: 2789 2789
Size: 6439
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6439
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6439
SmaI CCC'GGG
Cuts at: 1872 3766 1872
Size: 1894 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3586 4543 5444 5706 820
Size: 197 926 1643 957 901 262 1553
Fragments arranged by size:
1643 1553 957 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4230 493
Size: 3737 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6439
SphI G_CATG'C
Cuts at: 1132 3797 1132
Size: 2665 3774
SspI AAT'ATT
Cuts at: 5 52 4363 5
Size: 47 4311 2081
Fragments arranged by size:
4311 2081 47
StuI AGG'CCT
Cuts at: 1517 2903 1517
Size: 1386 5053
StyI C'CwwG_G
Cuts at: 513 1065 2265 3267 3421 3483 3878 513
Size: 552 1200 1002 154 62 395 3074
Fragments arranged by size:
3074 1200 1002 552 395 154 62
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2158 4466 4483 4636 4821 5266 325
Size: 1255 578 2308 17 153 185 445 1498
Fragments arranged by size:
2308 1498 1255 578 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4685 6397 96
Size: 274 80 33 51 1443 2708 1712 138
Fragments arranged by size:
2708 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6399 6431 6399
Size: 32 6407
Tth111I GACn'n_nGTC
Cuts at: 1316 2239 3698 1316
Size: 923 1459 4057
Fragments arranged by size:
4057 1459 923
XbaI T'CTAG_A
Cuts at: 3508 3775 3508
Size: 267 6172
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2449 3203 3546 2449
Size: 754 343 5342
Fragments arranged by size:
5342 754 343
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6439
XmaI C'CCGG_G
Cuts at: 1870 3764 1870
Size: 1894 4545
XmnI GAAnn'nnTTC
Cuts at: 4568 4568
Size: 6439
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4247 276
Size: 53 83 186 936 2713 2468
Fragments arranged by size:
2713 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BfrBI BsiWI BsmBI
BstZ17I EcoICRI EcoRV FalI FseI FspAI NruI NsiI
PacI PmlI PpuMI PspXI PsrI RsrII SacI SacII
SanDI SfiI SrfI SwaI