Restriction map for
pFN21AE1371
(Circular) (Six-base) MAPSORT of: pFN21AE1371.seq Check: 5079 from: 1 to: 5290
pFN21AE1371 5290 bp
With 182 enzymes: SgfI *
August 16, 2010 14:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3100 278
Size: 53 83 186 936 1564 2468
Fragments arranged by size:
2468 1564 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2633 1937
Size: 696 4594
Acc65I G'GTAC_C
Cuts at: 2611 2611
Size: 5290
AclI AA'CG_TT
Cuts at: 1563 3417 3790 1563
Size: 1854 373 3063
Fragments arranged by size:
3063 1854 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3353 4737 5005 1632
Size: 1721 1384 268 1917
Fragments arranged by size:
1917 1721 1384 268
AfeI AGC'GCT
Cuts at: 2204 2204
Size: 5290
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5093
AflIII A'CryG_T
Cuts at: 1203 4189 5166 1203
Size: 2986 977 1327
Fragments arranged by size:
2986 1327 977
AgeI A'CCGG_T
Cuts at: 4121 4121
Size: 5290
AhdI GACnn_n'nnGTC
Cuts at: 4019 4019
Size: 5290
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5290
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5290
AlwNI CAG_nnn'CTG
Cuts at: 2047 3033 4605 2047
Size: 986 1572 2732
Fragments arranged by size:
2732 1572 986
ApaI G_GGCC'C
Cuts at: 2250 2250
Size: 5290
ApaLI G'TGCA_C
Cuts at: 3347 4503 3347
Size: 1156 4134
ApoI r'AATT_y
Cuts at: 1493 2599 2825 2977 5086 1493
Size: 1106 226 152 2109 1697
Fragments arranged by size:
2109 1697 1106 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3844 160
Size: 872 2812 1606
Fragments arranged by size:
2812 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5290
AvaI C'yCGr_G
Cuts at: 1870 1943 2169 2418 2542 2615 1870
Size: 73 226 249 124 73 4545
Fragments arranged by size:
4545 249 226 124 73 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2104 2137 2104
Size: 33 5257
BamHI G'GATC_C
Cuts at: 2620 2987 2620
Size: 367 4923
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2120 2299 2441 2611 4066
Size: 325 217 627 333 179 142 170 1455
Cuts at: 4066 618
Size: 1842
Fragments arranged by size:
1842 1455 627 333 325 217 179 170
142
BanII G_rGCy'C
Cuts at: 1391 2192 2250 2412 2609 1391
Size: 801 58 162 197 4072
Fragments arranged by size:
4072 801 197 162 58
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4319
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3481 3515 1903
Size: 34 1544 34 3678
Fragments arranged by size:
3678 1544 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3182 4392 3182
Size: 1210 4080
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5290
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2030 2345 3901
Size: 107 122 71 631 605 358 315 1556
Cuts at: 3901 136
Size: 1525
Fragments arranged by size:
1556 1525 631 605 358 315 122 107
71
BglII A'GATC_T
Cuts at: 5285 5285
Size: 5290
BlpI GC'TnA_GC
Cuts at: 2707 2707
Size: 5290
Bme1580I G_kGCm'C
Cuts at: 1790 2250 2304 3351 4507 1790
Size: 460 54 1047 1156 2573
Fragments arranged by size:
2573 1156 1047 460 54
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5290
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2101 3021 3979
Size: 416 53 83 399 24 684 920 958
Cuts at: 3979 442
Size: 1753
Fragments arranged by size:
1753 958 920 684 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5290
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2180 3950 1239
Size: 941 1770 2579
Fragments arranged by size:
2579 1770 941
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3415 4280 4578 3415
Size: 865 298 4127
Fragments arranged by size:
4127 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3953 882
Size: 617 2454 2219
Fragments arranged by size:
2454 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3081 493
Size: 711 1877 2702
Fragments arranged by size:
2702 1877 711
BsaBI GATnn'nnATC
Cuts at: 2066 5284 2066
Size: 3218 2072
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2051 2399 3097 3479
Size: 53 83 186 936 518 348 698 382
Cuts at: 3479 4114 275
Size: 635 1451
Fragments arranged by size:
1451 936 698 635 518 382 348 186
83 53
BsaWI w'CCGG_w
Cuts at: 3723 4121 4395 4542 4953 3723
Size: 398 274 147 411 4060
Fragments arranged by size:
4060 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5065
Fragments arranged by size:
5065 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2525 2528 1186
Size: 1339 3 3948
Fragments arranged by size:
3948 1339 3
BseYI C'CCAG_C
Cuts at: 2093 2205 2459 2504 4493 4989 2093
Size: 112 254 45 1989 496 2394
Fragments arranged by size:
2394 1989 496 254 112 45
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4717
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2429 2487 2740 3501 4529 1540
Size: 275 186 428 58 253 761 1028 2301
Fragments arranged by size:
2301 1028 761 428 275 253 186 58
BsiHKAI G_wGCw'C
Cuts at: 3351 3436 4507 3351
Size: 85 1071 4134
Fragments arranged by size:
4134 1071 85
BsmI GAATG_Cn'
Cuts at: 2801 2894 2801
Size: 93 5197
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2192 2250 2304 2412 2609 3351 3436
Size: 399 402 58 54 108 197 742 85
Cuts at: 3436 4507 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 402 399 197 108 85
58 54
BspEI T'CCGG_A
Cuts at: 4953 4953
Size: 5290
BspHI T'CATG_A
Cuts at: 3179 4909 3179
Size: 1730 3560
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2645 844
Size: 1801 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2358 2425 3177 1394
Size: 429 535 67 752 3507
Fragments arranged by size:
3507 752 535 429 67
BsrDI GCAATG_nn'
Cuts at: 65 1248 2349 3785 3959 65
Size: 1183 1101 1436 174 1396
Fragments arranged by size:
1436 1396 1183 1101 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2306 3934 4121 1700
Size: 110 496 1628 187 2869
Fragments arranged by size:
2869 1628 496 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5290
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5290
BssSI C'ACGA_G
Cuts at: 3350 4362 3350
Size: 1012 4278
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2290 794
Size: 1496 3794
BstBI TT'CG_AA
Cuts at: 4094 4094
Size: 5290
BstEII G'GTnAC_C
Cuts at: 1184 2572 1184
Size: 1388 3902
BstYI r'GATC_y
Cuts at: 1972 2620 2987 3372 3389 4841 4927 4939 5285
Size: 648 367 385 17 1452 86 12 346
Cuts at: 5285 1972
Size: 1977
Fragments arranged by size:
1977 1452 648 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2196 2229 2474 513
Size: 552 939 192 33 245 3329
Fragments arranged by size:
3329 939 552 245 192 33
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4551
Fragments arranged by size:
4551 683 56
BtsI GCAGTG_nn'
Cuts at: 2399 2806 3600 3620 2399
Size: 407 794 20 4069
Fragments arranged by size:
4069 794 407 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5290
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2311 2346 529
Size: 35 1747 35 3473
Fragments arranged by size:
3473 1747 35 35
DraI TTT'AAA
Cuts at: 2594 2594
Size: 5290
DraIII CAC_nnn'GTG
Cuts at: 2109 2109
Size: 5290
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4297 809
Size: 507 2981 1802
Fragments arranged by size:
2981 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2085 2319 2346
Size: 54 1425 50 275 18 255 234 27
Cuts at: 2346 2426 2484 2737 3626 5255 8
Size: 80 58 253 889 1629 43
Fragments arranged by size:
1629 1425 889 275 255 253 234 80
58 54 50 43 27 18
EagI C'GGCC_G
Cuts at: 1537 1812 2426 2484 2737 1537
Size: 275 614 58 253 4090
Fragments arranged by size:
4090 614 275 253 58
EarI CTCTTCn'nnn_
Cuts at: 1353 2171 3220 1353
Size: 818 1049 3423
Fragments arranged by size:
3423 1049 818
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3877 4251 4397 387
Size: 1064 138 2288 374 146 1280
Fragments arranged by size:
2288 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2180 3353 3950 4737 5005 1239
Size: 393 548 1173 597 787 268 1524
Fragments arranged by size:
1524 1173 787 597 548 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2254 2254
Size: 5290
EcoO109I rG'GnC_Cy
Cuts at: 2247 2537 2549 2247
Size: 290 12 4988
Fragments arranged by size:
4988 290 12
EcoRI G'AATT_C
Cuts at: 2599 2977 2599
Size: 378 4912
FspI TGC'GCA
Cuts at: 3796 3796
Size: 5290
HaeII r_GCGC'y
Cuts at: 1678 2048 2101 2206 4437 1678
Size: 370 53 105 2231 2531
Fragments arranged by size:
2531 2231 370 105 53
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2430 2462 3931 3963 4005
Size: 32 135 32 858 32 1469 32 42
Cuts at: 4005 4037 1373
Size: 32 2626
Fragments arranged by size:
2626 1469 858 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2560 2634 2880 669
Size: 988 281 622 74 246 3079
Fragments arranged by size:
3079 988 622 281 246 74
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5290
HpaI GTT'AAC
Cuts at: 2880 2880
Size: 5290
KpnI G_GTAC'C
Cuts at: 2615 2615
Size: 5290
MfeI C'AATT_G
Cuts at: 2889 2889
Size: 5290
MluI A'CGCG_T
Cuts at: 5166 5166
Size: 5290
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4404 4588 1305
Size: 3099 184 2007
Fragments arranged by size:
3099 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2087 2321 2348 10
Size: 54 1425 343 255 234 27 2952
Fragments arranged by size:
2952 1425 343 255 234 54 27
MslI CAynn'nnrTG
Cuts at: 518 1166 2570 3248 3607 3766 518
Size: 648 1404 678 359 159 2042
Fragments arranged by size:
2042 1404 678 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2093 2476 2706 3383 4531 4776 1606
Size: 487 383 230 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 487 383 245 230
NaeI GCC'GGC
Cuts at: 2308 2308
Size: 5290
NcoI C'CATG_G
Cuts at: 513 1065 2004 2196 513
Size: 552 939 192 3607
Fragments arranged by size:
3607 939 552 192
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5290
NgoMIV G'CCGG_C
Cuts at: 2306 2306
Size: 5290
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5290
NotI GC'GGCC_GC
Cuts at: 2426 2737 2426
Size: 311 4979
NspI r_CATG'y
Cuts at: 1132 1229 2648 4193 1132
Size: 97 1419 1545 2229
Fragments arranged by size:
2229 1545 1419 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5290
PciI A'CATG_T
Cuts at: 4189 4189
Size: 5290
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5290
PmeI GTTT'AAAC
Cuts at: 2594 2594
Size: 5290
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3359 3391 4872 4904 3359
Size: 32 1481 32 3745
Fragments arranged by size:
3745 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2537 2549 2537
Size: 12 5278
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5290
PsiI TTA'TAA
Cuts at: 2860 2860
Size: 5290
PspOMI G'GGCC_C
Cuts at: 2246 2246
Size: 5290
PstI C_TGCA'G
Cuts at: 830 2642 830
Size: 1812 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5290
PvuII CAG'CTG
Cuts at: 2093 2093
Size: 5290
SacII CC_GC'GG
Cuts at: 2477 2477
Size: 5290
SalI G'TCGA_C
Cuts at: 1936 2632 1936
Size: 696 4594
SanDI GG'GwC_CC
Cuts at: 2537 2537
Size: 5290
SbfI CC_TGCA'GG
Cuts at: 2642 2642
Size: 5290
ScaI AGT'ACT
Cuts at: 3538 5250 3538
Size: 1712 3578
SfcI C'TryA_G
Cuts at: 826 1045 2638 3773 4454 4645 826
Size: 219 1593 1135 681 191 1471
Fragments arranged by size:
1593 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2030 2030
Size: 5290
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5290
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5290
SmaI CCC'GGG
Cuts at: 1872 2171 2420 2544 2617 1872
Size: 299 249 124 73 4545
Fragments arranged by size:
4545 299 249 124 73
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3394 4295 4557 820
Size: 197 926 1451 901 262 1553
Fragments arranged by size:
1553 1451 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3081 493
Size: 2588 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5290
SphI G_CATG'C
Cuts at: 1132 2648 1132
Size: 1516 3774
SspI AAT'ATT
Cuts at: 5 52 3214 5
Size: 47 3162 2081
Fragments arranged by size:
3162 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5290
StyI C'CwwG_G
Cuts at: 513 1065 2004 2196 2329 2729 513
Size: 552 939 192 133 400 3074
Fragments arranged by size:
3074 939 552 400 192 133
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2120 2591 3317 3334 3487 3672 4117
Size: 1255 540 471 726 17 153 185 445
Cuts at: 4117 325
Size: 1498
Fragments arranged by size:
1498 1255 726 540 471 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2217 3536 5248
Size: 274 80 33 51 1443 240 1319 1712
Cuts at: 5248 96
Size: 138
Fragments arranged by size:
1712 1443 1319 274 240 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5250 5282 5250
Size: 32 5258
Tth111I GACn'n_nGTC
Cuts at: 1316 2582 1316
Size: 1266 4024
XbaI T'CTAG_A
Cuts at: 2626 2626
Size: 5290
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2114 2114
Size: 5290
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5290
XmaI C'CCGG_G
Cuts at: 1870 2169 2418 2542 2615 1870
Size: 299 249 124 73 4545
Fragments arranged by size:
4545 299 249 124 73
XmnI GAAnn'nnTTC
Cuts at: 2240 3419 2240
Size: 1179 4111
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3098 276
Size: 53 83 186 936 1564 2468
Fragments arranged by size:
2468 1564 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI
SanDI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BbeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoRV
FalI FseI FspAI KasI NarI NruI NsiI PacI
PfoI PmlI PspXI PsrI RsrII SacI SapI SexAI
SfoI SrfI SwaI