Restriction map for
pFN21AE1369
(Circular) (Six-base) MAPSORT of: pFN21AE1369.seq Check: 5998 from: 1 to: 6319
pFN21AE1369 6319 bp
With 182 enzymes: SgfI *
August 16, 2010 14:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4129 278
Size: 53 83 186 936 2593 2468
Fragments arranged by size:
2593 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3351 3662 1937
Size: 1414 311 4594
Fragments arranged by size:
4594 1414 311
Acc65I G'GTAC_C
Cuts at: 2829 3640 2829
Size: 811 5508
AclI AA'CG_TT
Cuts at: 1563 4446 4819 1563
Size: 2883 373 3063
Fragments arranged by size:
3063 2883 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4382 5766 6034 1632
Size: 2750 1384 268 1917
Fragments arranged by size:
2750 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6122
AflIII A'CryG_T
Cuts at: 1203 5218 6195 1203
Size: 4015 977 1327
Fragments arranged by size:
4015 1327 977
AgeI A'CCGG_T
Cuts at: 2873 5150 2873
Size: 2277 4042
AhdI GACnn_n'nnGTC
Cuts at: 5048 5048
Size: 6319
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6319
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2447 3069 1184
Size: 1263 622 4434
Fragments arranged by size:
4434 1263 622
AlwNI CAG_nnn'CTG
Cuts at: 3067 4062 5634 3067
Size: 995 1572 3752
Fragments arranged by size:
3752 1572 995
ApaI G_GGCC'C
Cuts at: 2151 2757 2974 2151
Size: 606 217 5496
Fragments arranged by size:
5496 606 217
ApaLI G'TGCA_C
Cuts at: 4376 5532 4376
Size: 1156 5163
ApoI r'AATT_y
Cuts at: 1493 3628 3854 4006 6115 1493
Size: 2135 226 152 2109 1697
Fragments arranged by size:
2135 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4873 160
Size: 872 3841 1606
Fragments arranged by size:
3841 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6319
AvaI C'yCGr_G
Cuts at: 1870 1943 2096 2151 2196 2292 2418 2908 2967
Size: 73 153 55 45 96 126 490 59
Cuts at: 2967 3644 1870
Size: 677 4545
Fragments arranged by size:
4545 677 490 153 126 96 73 59
55 45
AvrII C'CTAG_G
Cuts at: 2135 2135
Size: 6319
BamHI G'GATC_C
Cuts at: 3649 4016 3649
Size: 367 5952
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2112 2248 2822 2829 2870
Size: 325 217 627 325 136 574 7 41
Cuts at: 2870 3011 3640 5095 618
Size: 141 629 1455 1842
Fragments arranged by size:
1842 1455 629 627 574 325 325 217
141 136 41 7
BanII G_rGCy'C
Cuts at: 1391 2151 2343 2531 2625 2725 2757 2896 2974
Size: 760 192 188 94 100 32 139 78
Cuts at: 2974 3638 1391
Size: 664 4072
Fragments arranged by size:
4072 760 664 192 188 139 100 94
78 32
BbeI G_GCGC'C
Cuts at: 2116 2252 2826 2116
Size: 136 574 5609
Fragments arranged by size:
5609 574 136
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5348
BbvCI CC'TCA_GC
Cuts at: 2357 2357
Size: 6319
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4510 4544 1903
Size: 34 2573 34 3678
Fragments arranged by size:
3678 2573 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2706 4211 5421 2706
Size: 1505 1210 3604
Fragments arranged by size:
3604 1505 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6319
BfrBI ATG'CAT
Cuts at: 3291 3291
Size: 6319
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2421 2542 2556
Size: 107 122 71 631 605 749 121 14
Cuts at: 2556 2969 3062 3315 4930 136
Size: 413 93 253 1615 1525
Fragments arranged by size:
1615 1525 749 631 605 413 253 122
121 107 93 71 14
BglII A'GATC_T
Cuts at: 6314 6314
Size: 6319
BlpI GC'TnA_GC
Cuts at: 2220 2652 3736 2220
Size: 432 1084 4803
Fragments arranged by size:
4803 1084 432
Bme1580I G_kGCm'C
Cuts at: 1790 2151 2757 2873 2974 3555 4380 5536 1790
Size: 361 606 116 101 581 825 1156 2573
Fragments arranged by size:
2573 1156 825 606 581 361 116 101
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6319
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2172 3082 4050
Size: 416 53 83 399 24 755 910 968
Cuts at: 4050 5008 442
Size: 958 1753
Fragments arranged by size:
1753 968 958 910 755 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6319
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2060 2357 2481 2739 2951 4979 1239
Size: 821 297 124 258 212 2028 2579
Fragments arranged by size:
2579 2028 821 297 258 212 124
Bpu10I CC'TnA_GC
Cuts at: 2357 2357
Size: 6319
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4444 5309 5607 4444
Size: 865 298 5156
Fragments arranged by size:
5156 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4982 882
Size: 617 3483 2219
Fragments arranged by size:
3483 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4110 493
Size: 711 2906 2702
Fragments arranged by size:
2906 2702 711
BsaBI GATnn'nnATC
Cuts at: 6313 6313
Size: 6319
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2113 2249 2823 4126
Size: 53 83 186 936 580 136 574 1303
Cuts at: 4126 4508 5143 275
Size: 382 635 1451
Fragments arranged by size:
1451 1303 936 635 580 574 382 186
136 83 53
BsaWI w'CCGG_w
Cuts at: 2873 2956 4752 5150 5424 5571 5982 2873
Size: 83 1796 398 274 147 411 3210
Fragments arranged by size:
3210 1796 411 398 274 147 83
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3399 3429 1178
Size: 30 165 30 1996 30 4068
Fragments arranged by size:
4068 1996 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2169 2948 1186
Size: 983 779 4557
Fragments arranged by size:
4557 983 779
BseYI C'CCAG_C
Cuts at: 5522 6018 5522
Size: 496 5823
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3414 1163
Size: 573 1678 4068
Fragments arranged by size:
4068 1678 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2053 3769 4530 5558 1540
Size: 275 186 52 1716 761 1028 2301
Fragments arranged by size:
2301 1716 1028 761 275 186 52
BsiHKAI G_wGCw'C
Cuts at: 2578 2625 2725 4380 4465 5536 2578
Size: 47 100 1655 85 1071 3361
Fragments arranged by size:
3361 1655 1071 100 85 47
BsmI GAATG_Cn'
Cuts at: 3830 3923 3830
Size: 93 6226
BsmBI CGTCTCn'nnnn_
Cuts at: 2758 2758
Size: 6319
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2151 2343 2531 2578 2625 2725 2757
Size: 399 361 192 188 47 47 100 32
Cuts at: 2757 2873 2896 2974 3555 3638 4380 4465 5536
Size: 116 23 78 581 83 742 85 1071
Cuts at: 5536 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 581 399 361 192 188
116 100 85 83 78 47 47 32
23
BspEI T'CCGG_A
Cuts at: 2956 5982 2956
Size: 3026 3293
BspHI T'CATG_A
Cuts at: 4208 5938 4208
Size: 1730 4589
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3674 844
Size: 2830 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2011 2367 2747 4206 1394
Size: 429 188 356 380 1459 3507
Fragments arranged by size:
3507 1459 429 380 356 188
BsrDI GCAATG_nn'
Cuts at: 65 1248 3058 3261 4814 4988 65
Size: 1183 1810 203 1553 174 1396
Fragments arranged by size:
1810 1553 1396 1183 203 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2073 2191 2469 2578 2798 2873 4963
Size: 110 263 118 278 109 220 75 2090
Cuts at: 4963 5150 1700
Size: 187 2869
Fragments arranged by size:
2869 2090 278 263 220 187 118 110
109 75
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6319
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6319
BssSI C'ACGA_G
Cuts at: 4379 5391 4379
Size: 1012 5307
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3057 3297 3306 794
Size: 2263 240 9 3807
Fragments arranged by size:
3807 2263 240 9
BstBI TT'CG_AA
Cuts at: 5123 5123
Size: 6319
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6319
BstYI r'GATC_y
Cuts at: 1972 3243 3649 4016 4401 4418 5870 5956 5968
Size: 1271 406 367 385 17 1452 86 12
Cuts at: 5968 6314 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1271 406 385 367 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 3352 3352
Size: 6319
BtgI C'CryG_G
Cuts at: 513 1065 2004 2274 2343 2521 2661 2817 2896
Size: 552 939 270 69 178 140 156 79
Cuts at: 2896 3456 3565 513
Size: 560 109 3267
Fragments arranged by size:
3267 939 560 552 270 178 156 140
109 79 69
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5580
Fragments arranged by size:
5580 683 56
BtsI GCAGTG_nn'
Cuts at: 3835 4629 4649 3835
Size: 794 20 5505
Fragments arranged by size:
5505 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6319
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6284
DraI TTT'AAA
Cuts at: 3623 3623
Size: 6319
DraIII CAC_nnn'GTG
Cuts at: 2794 3090 2794
Size: 296 6023
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5326 809
Size: 507 4010 1802
Fragments arranged by size:
4010 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2050 2388 2568
Size: 54 1425 50 275 18 220 338 180
Cuts at: 2568 3403 3766 4655 6284 8
Size: 835 363 889 1629 43
Fragments arranged by size:
1629 1425 889 835 363 338 275 220
180 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2050 3766 1537
Size: 275 238 1716 4090
Fragments arranged by size:
4090 1716 275 238
EarI CTCTTCn'nnn_
Cuts at: 1353 4249 1353
Size: 2896 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2469 2599 4906 5280 5426 387
Size: 1064 138 880 130 2307 374 146 1280
Fragments arranged by size:
2307 1280 1064 880 374 146 138 130
EcoICRI GAG'CTC
Cuts at: 2623 2723 2623
Size: 100 6219
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2060 2357 2481 2739 2951 4382 4979
Size: 393 428 297 124 258 212 1431 597
Cuts at: 4979 5766 6034 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1431 787 597 428 393 297 268
258 212 124
EcoO109I rG'GnC_Cy
Cuts at: 2091 2132 2147 2148 2168 2602 2753 2754 2945
Size: 41 15 1 20 434 151 1 191
Cuts at: 2945 3064 2091
Size: 119 5346
Fragments arranged by size:
5346 434 191 151 119 41 20 15
1 1
EcoRI G'AATT_C
Cuts at: 3628 4006 3628
Size: 378 5941
EcoRV GAT'ATC
Cuts at: 3453 3453
Size: 6319
FspI TGC'GCA
Cuts at: 4825 4825
Size: 6319
HaeII r_GCGC'y
Cuts at: 1678 2116 2252 2521 2826 5466 1678
Size: 438 136 269 305 2640 2531
Fragments arranged by size:
2640 2531 438 305 269 136
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4960 4992 5034 5066 1373
Size: 32 135 32 3388 32 42 32 2626
Fragments arranged by size:
3388 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3663 3909 669
Size: 988 281 1725 246 3079
Fragments arranged by size:
3079 1725 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6319
HpaI GTT'AAC
Cuts at: 3909 3909
Size: 6319
KasI G'GCGC_C
Cuts at: 2112 2248 2822 2112
Size: 136 574 5609
Fragments arranged by size:
5609 574 136
KpnI G_GTAC'C
Cuts at: 2833 3644 2833
Size: 811 5508
MfeI C'AATT_G
Cuts at: 3918 3918
Size: 6319
MluI A'CGCG_T
Cuts at: 6195 6195
Size: 6319
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2166 3366 5433 5617 1305
Size: 861 1200 2067 184 2007
Fragments arranged by size:
2067 2007 1200 861 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3405 10
Size: 54 1425 343 1573 2924
Fragments arranged by size:
2924 1573 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2501 4277 4636 4795 518
Size: 648 1335 1776 359 159 2042
Fragments arranged by size:
2042 1776 1335 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2276 2493 2540 2656 2663 2898 3203 3511
Size: 670 217 47 116 7 235 305 308
Cuts at: 3511 3583 3735 4412 5560 5805 1606
Size: 72 152 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 670 308 305 245 235
217 152 116 72 47 7
NaeI GCC'GGC
Cuts at: 2193 2471 2193
Size: 278 6041
NarI GG'CG_CC
Cuts at: 2113 2249 2823 2113
Size: 136 574 5609
Fragments arranged by size:
5609 574 136
NcoI C'CATG_G
Cuts at: 513 1065 2004 3456 3565 513
Size: 552 939 1452 109 3267
Fragments arranged by size:
3267 1452 939 552 109
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6319
NgoMIV G'CCGG_C
Cuts at: 2191 2469 2191
Size: 278 6041
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6319
NotI GC'GGCC_GC
Cuts at: 3766 3766
Size: 6319
NsiI A_TGCA'T
Cuts at: 3293 3293
Size: 6319
NspI r_CATG'y
Cuts at: 1132 1229 3580 3677 5222 1132
Size: 97 2351 97 1545 2229
Fragments arranged by size:
2351 2229 1545 97 97
PasI CC'CwG_GG
Cuts at: 1348 2707 1348
Size: 1359 4960
PciI A'CATG_T
Cuts at: 5218 5218
Size: 6319
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3067 1108
Size: 1959 4360
PfoI T'CCnGG_A
Cuts at: 2593 2593
Size: 6319
PmeI GTTT'AAAC
Cuts at: 3623 3623
Size: 6319
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4388 4420 5901 5933 4388
Size: 32 1481 32 4774
Fragments arranged by size:
4774 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2091 2132 2168 2602 2945 2091
Size: 41 36 434 343 5465
Fragments arranged by size:
5465 434 343 41 36
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6319
PsiI TTA'TAA
Cuts at: 3889 3889
Size: 6319
PspOMI G'GGCC_C
Cuts at: 2147 2753 2970 2147
Size: 606 217 5496
Fragments arranged by size:
5496 606 217
PstI C_TGCA'G
Cuts at: 830 2480 3671 830
Size: 1650 1191 3478
Fragments arranged by size:
3478 1650 1191
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6319
PvuII CAG'CTG
Cuts at: 3203 3511 3203
Size: 308 6011
SacI G_AGCT'C
Cuts at: 2625 2725 2625
Size: 100 6219
SacII CC_GC'GG
Cuts at: 2277 2664 2899 2277
Size: 387 235 5697
Fragments arranged by size:
5697 387 235
SalI G'TCGA_C
Cuts at: 1936 3661 1936
Size: 1725 4594
SanDI GG'GwC_CC
Cuts at: 2091 2602 2091
Size: 511 5808
SbfI CC_TGCA'GG
Cuts at: 3671 3671
Size: 6319
ScaI AGT'ACT
Cuts at: 4567 6279 4567
Size: 1712 4607
SexAI A'CCwGG_T
Cuts at: 2941 2941
Size: 6319
SfcI C'TryA_G
Cuts at: 826 1045 2476 3139 3667 4802 5483 5674 826
Size: 219 1431 663 528 1135 681 191 1471
Fragments arranged by size:
1471 1431 1135 681 663 528 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2969 2969
Size: 6319
SfoI GGC'GCC
Cuts at: 2114 2250 2824 2114
Size: 136 574 5609
Fragments arranged by size:
5609 574 136
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6319
SgrAI Cr'CCGG_yG
Cuts at: 1700 2873 1700
Size: 1173 5146
SmaI CCC'GGG
Cuts at: 1872 2098 2198 2294 2420 2969 3646 1872
Size: 226 100 96 126 549 677 4545
Fragments arranged by size:
4545 677 549 226 126 100 96
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4423 5324 5586 820
Size: 197 926 2480 901 262 1553
Fragments arranged by size:
2480 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4110 493
Size: 3617 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6319
SphI G_CATG'C
Cuts at: 1132 3677 1132
Size: 2545 3774
SrfI GCCC'GGGC
Cuts at: 2969 2969
Size: 6319
SspI AAT'ATT
Cuts at: 5 52 4243 5
Size: 47 4191 2081
Fragments arranged by size:
4191 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6319
StyI C'CwwG_G
Cuts at: 513 1065 2004 2135 2757 3456 3565 3758 513
Size: 552 939 131 622 699 109 193 3074
Fragments arranged by size:
3074 939 699 622 552 193 131 109
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2655 3101 4346 4363 4516 4701 5146
Size: 1255 1075 446 1245 17 153 185 445
Cuts at: 5146 325
Size: 1498
Fragments arranged by size:
1498 1255 1245 1075 446 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3159 4565 6277
Size: 274 80 33 51 1443 1182 1406 1712
Cuts at: 6277 96
Size: 138
Fragments arranged by size:
1712 1443 1406 1182 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6279 6311 6279
Size: 32 6287
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6319
XbaI T'CTAG_A
Cuts at: 3655 3655
Size: 6319
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2035 2035
Size: 6319
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6319
XmaI C'CCGG_G
Cuts at: 1870 2096 2196 2292 2418 2967 3644 1870
Size: 226 100 96 126 549 677 4545
Fragments arranged by size:
4545 677 549 226 126 100 96
XmnI GAAnn'nnTTC
Cuts at: 4448 4448
Size: 6319
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4127 276
Size: 53 83 186 936 2593 2468
Fragments arranged by size:
2593 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SacII SalI SanDI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BaeI BplI BsiWI BstXI
Bsu36I EcoNI FalI FseI FspAI NruI PacI PmlI
PspXI PsrI RsrII SapI SwaI