Restriction map for
pFN21AE1367
(Circular) (Six-base) MAPSORT of: pFN21AE1367.seq Check: 917 from: 1 to: 5815
pFN21AE1367 5815 bp
With 182 enzymes: SgfI *
August 16, 2010 14:46 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3625 278
Size: 53 83 186 936 2089 2468
Fragments arranged by size:
2468 2089 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3158 1937
Size: 1221 4594
Acc65I G'GTAC_C
Cuts at: 3136 3136
Size: 5815
AclI AA'CG_TT
Cuts at: 1563 3942 4315 1563
Size: 2379 373 3063
Fragments arranged by size:
3063 2379 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2212 3089 3878 5262 5530 1632
Size: 580 877 789 1384 268 1917
Fragments arranged by size:
1917 1384 877 789 580 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5618
AflIII A'CryG_T
Cuts at: 1203 4714 5691 1203
Size: 3511 977 1327
Fragments arranged by size:
3511 1327 977
AgeI A'CCGG_T
Cuts at: 4646 4646
Size: 5815
AhdI GACnn_n'nnGTC
Cuts at: 4544 4544
Size: 5815
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5815
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5815
AlwNI CAG_nnn'CTG
Cuts at: 2632 2782 3067 3558 5130 2632
Size: 150 285 491 1572 3317
Fragments arranged by size:
3317 1572 491 285 150
ApaI G_GGCC'C
Cuts at: 2074 2074
Size: 5815
ApaLI G'TGCA_C
Cuts at: 3872 5028 3872
Size: 1156 4659
ApoI r'AATT_y
Cuts at: 1493 2901 2936 3124 3350 3502 5611 1493
Size: 1408 35 188 226 152 2109 1697
Fragments arranged by size:
2109 1697 1408 226 188 152 35
AseI AT'TA_AT
Cuts at: 160 1032 4369 160
Size: 872 3337 1606
Fragments arranged by size:
3337 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5815
AvaI C'yCGr_G
Cuts at: 1870 1943 2084 3140 1870
Size: 73 141 1056 4545
Fragments arranged by size:
4545 1056 141 73
BamHI G'GATC_C
Cuts at: 3145 3512 3145
Size: 367 5448
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2054 2378 2656 3136 4591
Size: 325 217 627 267 324 278 480 1455
Cuts at: 4591 618
Size: 1842
Fragments arranged by size:
1842 1455 627 480 325 324 278 267
217
BanII G_rGCy'C
Cuts at: 1391 2074 2300 2426 3134 1391
Size: 683 226 126 708 4072
Fragments arranged by size:
4072 708 683 226 126
BbeI G_GCGC'C
Cuts at: 2058 2058
Size: 5815
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2154 928
Size: 971 255 4589
Fragments arranged by size:
4589 971 255
BbvCI CC'TCA_GC
Cuts at: 2030 2030
Size: 5815
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4006 4040 1903
Size: 34 2069 34 3678
Fragments arranged by size:
3678 2069 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3707 4917 3707
Size: 1210 4605
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5815
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2507 2831
Size: 107 122 71 631 605 337 498 324
Cuts at: 2831 4426 136
Size: 1595 1525
Fragments arranged by size:
1595 1525 631 605 498 337 324 122
107 71
BglII A'GATC_T
Cuts at: 5810 5810
Size: 5815
BlpI GC'TnA_GC
Cuts at: 3232 3232
Size: 5815
Bme1580I G_kGCm'C
Cuts at: 1790 2074 3876 5032 1790
Size: 284 1802 1156 2573
Fragments arranged by size:
2573 1802 1156 284
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5815
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2172 2199 2620
Size: 416 53 83 399 24 755 27 421
Cuts at: 2620 2792 3546 4504 442
Size: 172 754 958 1753
Fragments arranged by size:
1753 958 755 754 421 416 399 172
83 53 27 24
BmtI G_CTAG'C
Cuts at: 1056 2120 2681 1056
Size: 1064 561 4190
Fragments arranged by size:
4190 1064 561
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2697 2697
Size: 5815
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2448 2654 2805 4475 1239
Size: 1209 206 151 1670 2579
Fragments arranged by size:
2579 1670 1209 206 151
Bpu10I CC'TnA_GC
Cuts at: 2030 2030
Size: 5815
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3940 4805 5103 3940
Size: 865 298 4652
Fragments arranged by size:
4652 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2226 2426 2440 4478 882
Size: 617 727 200 14 2038 2219
Fragments arranged by size:
2219 2038 727 617 200 14
BsaAI yAC'GTr
Cuts at: 493 1204 3606 493
Size: 711 2402 2702
Fragments arranged by size:
2702 2402 711
BsaBI GATnn'nnATC
Cuts at: 5809 5809
Size: 5815
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2055 3622 4004 4639
Size: 53 83 186 936 522 1567 382 635
Cuts at: 4639 275
Size: 1451
Fragments arranged by size:
1567 1451 936 635 522 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4248 4646 4920 5067 5478 4248
Size: 398 274 147 411 4585
Fragments arranged by size:
4585 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2803 2833 1178
Size: 30 165 30 1400 30 4160
Fragments arranged by size:
4160 1400 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2075 2276 1186
Size: 889 201 4725
Fragments arranged by size:
4725 889 201
BseYI C'CCAG_C
Cuts at: 2097 2291 5018 5514 2097
Size: 194 2727 496 2398
Fragments arranged by size:
2727 2398 496 194
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2951 3122 1163
Size: 573 1215 171 3856
Fragments arranged by size:
3856 1215 573 171
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2094 3265 4026 5054 1540
Size: 275 186 93 1171 761 1028 2301
Fragments arranged by size:
2301 1171 1028 761 275 186 93
BsiHKAI G_wGCw'C
Cuts at: 3876 3961 5032 3876
Size: 85 1071 4659
Fragments arranged by size:
4659 1071 85
BsmI GAATG_Cn'
Cuts at: 2527 3326 3419 2527
Size: 799 93 4923
Fragments arranged by size:
4923 799 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2074 2300 2426 3134 3876 3961 5032
Size: 399 284 226 126 708 742 85 1071
Cuts at: 5032 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 708 399 284 226 126
85
BspEI T'CCGG_A
Cuts at: 5478 5478
Size: 5815
BspHI T'CATG_A
Cuts at: 2242 3021 3704 5434 2242
Size: 779 683 1730 2623
Fragments arranged by size:
2623 1730 779 683
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3090 3170 844
Size: 2246 80 3489
Fragments arranged by size:
3489 2246 80
BsrBI CCG'CTC
Cuts at: 1394 1823 3702 1394
Size: 429 1879 3507
Fragments arranged by size:
3507 1879 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4310 4484 65
Size: 1183 3062 174 1396
Fragments arranged by size:
3062 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2093 4459 4646 1700
Size: 110 283 2366 187 2869
Fragments arranged by size:
2869 2366 283 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5815
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5815
BssSI C'ACGA_G
Cuts at: 3875 4887 3875
Size: 1012 4803
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5815
BstBI TT'CG_AA
Cuts at: 4619 4619
Size: 5815
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5815
BstXI CCAn_nnnn'nTGG
Cuts at: 2356 2356
Size: 5815
BstYI r'GATC_y
Cuts at: 1972 3145 3512 3897 3914 5366 5452 5464 5810
Size: 1173 367 385 17 1452 86 12 346
Cuts at: 5810 1972
Size: 1977
Fragments arranged by size:
1977 1452 1173 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2997 513
Size: 552 939 993 3331
Fragments arranged by size:
3331 993 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5076
Fragments arranged by size:
5076 683 56
BtsI GCAGTG_nn'
Cuts at: 2790 3331 4125 4145 2790
Size: 541 794 20 4460
Fragments arranged by size:
4460 794 541 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5815
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5780
DraI TTT'AAA
Cuts at: 2374 3119 2374
Size: 745 5070
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4822 809
Size: 507 3506 1802
Fragments arranged by size:
3506 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2091 2990 3262
Size: 54 1425 50 275 18 261 899 272
Cuts at: 3262 4151 5780 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 899 889 275 272 261 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2091 3262 1537
Size: 275 279 1171 4090
Fragments arranged by size:
4090 1171 279 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2262 2487 2561 2863 2978 3745 1353
Size: 909 225 74 302 115 767 3423
Fragments arranged by size:
3423 909 767 302 225 115 74
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2027 4402 4776 4922 387
Size: 1064 138 438 2375 374 146 1280
Fragments arranged by size:
2375 1280 1064 438 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2212 2448 2654 2805 3089 3878 4475
Size: 393 580 236 206 151 284 789 597
Cuts at: 4475 5262 5530 1239
Size: 787 268 1524
Fragments arranged by size:
1524 789 787 597 580 393 284 268
236 206 151
EcoRI G'AATT_C
Cuts at: 3124 3502 3124
Size: 378 5437
FspI TGC'GCA
Cuts at: 4321 4321
Size: 5815
HaeII r_GCGC'y
Cuts at: 1678 2030 2058 4962 1678
Size: 352 28 2904 2531
Fragments arranged by size:
2904 2531 352 28
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2480 2512 2961 2993 4456
Size: 32 135 32 908 32 449 32 1463
Cuts at: 4456 4488 4530 4562 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1463 908 449 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3111 3159 3405 669
Size: 988 281 1173 48 246 3079
Fragments arranged by size:
3079 1173 988 281 246 48
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5815
HpaI GTT'AAC
Cuts at: 3111 3405 3111
Size: 294 5521
KasI G'GCGC_C
Cuts at: 2054 2054
Size: 5815
KpnI G_GTAC'C
Cuts at: 3140 3140
Size: 5815
MfeI C'AATT_G
Cuts at: 3414 3414
Size: 5815
MluI A'CGCG_T
Cuts at: 5691 5691
Size: 5815
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2992 4929 5113 1305
Size: 1687 1937 184 2007
Fragments arranged by size:
2007 1937 1687 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3993
Fragments arranged by size:
3993 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3773 4132 4291 518
Size: 648 2607 359 159 2042
Fragments arranged by size:
2607 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3106 3231 3908 5056 5301 1606
Size: 1500 125 677 1148 245 2120
Fragments arranged by size:
2120 1500 1148 677 245 125
NaeI GCC'GGC
Cuts at: 2095 2095
Size: 5815
NarI GG'CG_CC
Cuts at: 2055 2055
Size: 5815
NcoI C'CATG_G
Cuts at: 513 1065 2004 2997 513
Size: 552 939 993 3331
Fragments arranged by size:
3331 993 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5815
NgoMIV G'CCGG_C
Cuts at: 2093 2093
Size: 5815
NheI G'CTAG_C
Cuts at: 1052 2116 2677 1052
Size: 1064 561 4190
Fragments arranged by size:
4190 1064 561
NotI GC'GGCC_GC
Cuts at: 3262 3262
Size: 5815
NspI r_CATG'y
Cuts at: 1132 1229 3173 4718 1132
Size: 97 1944 1545 2229
Fragments arranged by size:
2229 1944 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5815
PciI A'CATG_T
Cuts at: 4714 4714
Size: 5815
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2632 1108
Size: 1524 4291
PmeI GTTT'AAAC
Cuts at: 3119 3119
Size: 5815
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3884 3916 5397 5429 3884
Size: 32 1481 32 4270
Fragments arranged by size:
4270 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5815
PsiI TTA'TAA
Cuts at: 3385 3385
Size: 5815
PspOMI G'GGCC_C
Cuts at: 2070 2070
Size: 5815
PstI C_TGCA'G
Cuts at: 830 3167 830
Size: 2337 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5815
SalI G'TCGA_C
Cuts at: 1936 3157 1936
Size: 1221 4594
SapI GCTCTTCn'nnn_
Cuts at: 2863 2863
Size: 5815
SbfI CC_TGCA'GG
Cuts at: 3167 3167
Size: 5815
ScaI AGT'ACT
Cuts at: 4063 5775 4063
Size: 1712 4103
SfcI C'TryA_G
Cuts at: 826 1045 2397 3163 4298 4979 5170 826
Size: 219 1352 766 1135 681 191 1471
Fragments arranged by size:
1471 1352 1135 766 681 219 191
SfoI GGC'GCC
Cuts at: 2056 2056
Size: 5815
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5815
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5815
SmaI CCC'GGG
Cuts at: 1872 3142 1872
Size: 1270 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2084 3919 4820 5082 820
Size: 197 926 141 1835 901 262 1553
Fragments arranged by size:
1835 1553 926 901 262 197 141
SnaBI TAC'GTA
Cuts at: 493 3606 493
Size: 3113 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5815
SphI G_CATG'C
Cuts at: 1132 3173 1132
Size: 2041 3774
SspI AAT'ATT
Cuts at: 5 52 3739 5
Size: 47 3687 2081
Fragments arranged by size:
3687 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5815
StyI C'CwwG_G
Cuts at: 513 1065 2004 2473 2997 3254 513
Size: 552 939 469 524 257 3074
Fragments arranged by size:
3074 939 552 524 469 257
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3842 3859 4012 4197 4642 325
Size: 1255 2262 17 153 185 445 1498
Fragments arranged by size:
2262 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4061 5773 96
Size: 274 80 33 51 1443 2084 1712 138
Fragments arranged by size:
2084 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5775 5807 5775
Size: 32 5783
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5815
XbaI T'CTAG_A
Cuts at: 3151 3151
Size: 5815
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2356 2356
Size: 5815
XhoI C'TCGA_G
Cuts at: 1943 2084 1943
Size: 141 5674
XmaI C'CCGG_G
Cuts at: 1870 3140 1870
Size: 1270 4545
XmnI GAAnn'nnTTC
Cuts at: 3944 3944
Size: 5815
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3623 276
Size: 53 83 186 936 2089 2468
Fragments arranged by size:
2468 2089 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PspOMI PstI PvuI SalI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV
FalI FseI FspAI NruI NsiI PacI PfoI PmlI
PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SexAI SfiI SrfI SwaI