Restriction map for
pFN21AE1366
(Circular) (Six-base) MAPSORT of: pFN21AE1366.seq Check: 158 from: 1 to: 5596
pFN21AE1366 5596 bp
With 182 enzymes: SgfI *
August 16, 2010 14:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3406 278
Size: 53 83 186 936 1870 2468
Fragments arranged by size:
2468 1870 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2939 1937
Size: 1002 4594
Acc65I G'GTAC_C
Cuts at: 2259 2917 2259
Size: 658 4938
AclI AA'CG_TT
Cuts at: 1563 3723 4096 1563
Size: 2160 373 3063
Fragments arranged by size:
3063 2160 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2080 3659 5043 5311 1632
Size: 448 1579 1384 268 1917
Fragments arranged by size:
1917 1579 1384 448 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5399
AflIII A'CryG_T
Cuts at: 1203 4495 5472 1203
Size: 3292 977 1327
Fragments arranged by size:
3292 1327 977
AgeI A'CCGG_T
Cuts at: 4427 4427
Size: 5596
AhdI GACnn_n'nnGTC
Cuts at: 4325 4325
Size: 5596
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5596
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5596
AlwNI CAG_nnn'CTG
Cuts at: 3339 4911 3339
Size: 1572 4024
ApaI G_GGCC'C
Cuts at: 2123 2123
Size: 5596
ApaLI G'TGCA_C
Cuts at: 3653 4809 3653
Size: 1156 4440
ApoI r'AATT_y
Cuts at: 1493 2020 2362 2430 2461 2905 3131 3283 5392
Size: 527 342 68 31 444 226 152 2109
Cuts at: 5392 1493
Size: 1697
Fragments arranged by size:
2109 1697 527 444 342 226 152 68
31
AseI AT'TA_AT
Cuts at: 160 1032 4150 160
Size: 872 3118 1606
Fragments arranged by size:
3118 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5596
AvaI C'yCGr_G
Cuts at: 1870 1943 2921 1870
Size: 73 978 4545
Fragments arranged by size:
4545 978 73
BamHI G'GATC_C
Cuts at: 2926 3293 2926
Size: 367 5229
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2259 2917 4372 618
Size: 325 217 627 472 658 1455 1842
Fragments arranged by size:
1842 1455 658 627 472 325 217
BanII G_rGCy'C
Cuts at: 1391 2123 2187 2476 2915 1391
Size: 732 64 289 439 4072
Fragments arranged by size:
4072 732 439 289 64
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2100 2201 2790 928
Size: 971 201 101 589 3734
Fragments arranged by size:
3734 971 589 201 101
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3787 3821 1903
Size: 34 1850 34 3678
Fragments arranged by size:
3678 1850 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3488 4698 3488
Size: 1210 4386
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5596
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4207 136
Size: 107 122 71 631 605 2535 1525
Fragments arranged by size:
2535 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5591 5591
Size: 5596
BlpI GC'TnA_GC
Cuts at: 3013 3013
Size: 5596
Bme1580I G_kGCm'C
Cuts at: 1790 2123 3657 4813 1790
Size: 333 1534 1156 2573
Fragments arranged by size:
2573 1534 1156 333
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5596
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3327 4285 442
Size: 416 53 83 399 24 1910 958 1753
Fragments arranged by size:
1910 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5596
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2341 2548 2341
Size: 207 5389
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2311 2650 4256 1239
Size: 1072 339 1606 2579
Fragments arranged by size:
2579 1606 1072 339
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3721 4586 4884 3721
Size: 865 298 4433
Fragments arranged by size:
4433 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4259 882
Size: 617 2760 2219
Fragments arranged by size:
2760 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3387 493
Size: 711 2183 2702
Fragments arranged by size:
2702 2183 711
BsaBI GATnn'nnATC
Cuts at: 2597 5590 2597
Size: 2993 2603
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3403 3785 4420 275
Size: 53 83 186 936 1870 382 635 1451
Fragments arranged by size:
1870 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2294 4029 4427 4701 4848 5259 2294
Size: 1735 398 274 147 411 2631
Fragments arranged by size:
2631 1735 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5371
Fragments arranged by size:
5371 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5596
BseYI C'CCAG_C
Cuts at: 2221 4799 5295 2221
Size: 2578 496 2522
Fragments arranged by size:
2578 2522 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5023
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3046 3807 4835 1540
Size: 275 186 1045 761 1028 2301
Fragments arranged by size:
2301 1045 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2476 2666 3657 3742 4813 2476
Size: 190 991 85 1071 3259
Fragments arranged by size:
3259 1071 991 190 85
BsmI GAATG_Cn'
Cuts at: 3107 3200 3107
Size: 93 5503
BsmBI CGTCTCn'nnnn_
Cuts at: 2825 2825
Size: 5596
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2123 2187 2476 2666 2915 3657 3742
Size: 399 333 64 289 190 249 742 85
Cuts at: 3742 4813 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 333 289 249 190
85 64
BspEI T'CCGG_A
Cuts at: 2294 5259 2294
Size: 2965 2631
BspHI T'CATG_A
Cuts at: 2139 3485 5215 2139
Size: 1346 1730 2520
Fragments arranged by size:
2520 1730 1346
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2951 844
Size: 2107 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3483 1394
Size: 429 1660 3507
Fragments arranged by size:
3507 1660 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2193 4091 4265 65
Size: 1183 945 1898 174 1396
Fragments arranged by size:
1898 1396 1183 945 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4240 4427 1700
Size: 110 2430 187 2869
Fragments arranged by size:
2869 2430 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5596
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5596
BssSI C'ACGA_G
Cuts at: 3656 4668 3656
Size: 1012 4584
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5596
BstBI TT'CG_AA
Cuts at: 4400 4400
Size: 5596
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5596
BstYI r'GATC_y
Cuts at: 1972 2229 2926 3293 3678 3695 5147 5233 5245
Size: 257 697 367 385 17 1452 86 12
Cuts at: 5245 5591 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 697 385 367 346 257 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5044
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4857
Fragments arranged by size:
4857 683 56
BtsI GCAGTG_nn'
Cuts at: 3112 3906 3926 3112
Size: 794 20 4782
Fragments arranged by size:
4782 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5596
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5561
DraI TTT'AAA
Cuts at: 2445 2900 2445
Size: 455 5141
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4603 809
Size: 507 3287 1802
Fragments arranged by size:
3287 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3043 3932 5561
Size: 54 1425 50 275 18 1213 889 1629
Cuts at: 5561 8
Size: 43
Fragments arranged by size:
1629 1425 1213 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3043 1537
Size: 275 1231 4090
Fragments arranged by size:
4090 1231 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2099 2192 2805 3526 1353
Size: 746 93 613 721 3423
Fragments arranged by size:
3423 746 721 613 93
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4183 4557 4703 387
Size: 1064 138 2594 374 146 1280
Fragments arranged by size:
2594 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2474 2474
Size: 5596
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2080 2311 2650 3659 4256 5043 5311
Size: 393 448 231 339 1009 597 787 268
Cuts at: 5311 1239
Size: 1524
Fragments arranged by size:
1524 1009 787 597 448 393 339 268
231
EcoO109I rG'GnC_Cy
Cuts at: 2060 2120 2060
Size: 60 5536
EcoRI G'AATT_C
Cuts at: 2362 2905 3283 2362
Size: 543 378 4675
Fragments arranged by size:
4675 543 378
FspI TGC'GCA
Cuts at: 4102 4102
Size: 5596
HaeII r_GCGC'y
Cuts at: 1678 4743 1678
Size: 3065 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2849 2881 4237 4269 4311
Size: 32 135 32 1277 32 1356 32 42
Cuts at: 4311 4343 1373
Size: 32 2626
Fragments arranged by size:
2626 1356 1277 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2940 3186 669
Size: 988 281 1002 246 3079
Fragments arranged by size:
3079 1002 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2131 748
Size: 1383 4213
HpaI GTT'AAC
Cuts at: 3186 3186
Size: 5596
KpnI G_GTAC'C
Cuts at: 2263 2921 2263
Size: 658 4938
MfeI C'AATT_G
Cuts at: 3195 3195
Size: 5596
MluI A'CGCG_T
Cuts at: 5472 5472
Size: 5596
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4710 4894 1305
Size: 3405 184 2007
Fragments arranged by size:
3405 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3774
Fragments arranged by size:
3774 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2246 3554 3913 4072 518
Size: 648 1080 1308 359 159 2042
Fragments arranged by size:
2042 1308 1080 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3012 3689 4837 5082 1606
Size: 1406 677 1148 245 2120
Fragments arranged by size:
2120 1406 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5044
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5596
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5596
NotI GC'GGCC_GC
Cuts at: 3043 3043
Size: 5596
NspI r_CATG'y
Cuts at: 1132 1229 2954 4499 1132
Size: 97 1725 1545 2229
Fragments arranged by size:
2229 1725 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2200 1348
Size: 852 4744
PciI A'CATG_T
Cuts at: 4495 4495
Size: 5596
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5596
PfoI T'CCnGG_A
Cuts at: 2327 2613 2327
Size: 286 5310
PmeI GTTT'AAAC
Cuts at: 2900 2900
Size: 5596
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3665 3697 5178 5210 3665
Size: 32 1481 32 4051
Fragments arranged by size:
4051 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5596
PsiI TTA'TAA
Cuts at: 3166 3166
Size: 5596
PspOMI G'GGCC_C
Cuts at: 2119 2119
Size: 5596
PstI C_TGCA'G
Cuts at: 830 2948 830
Size: 2118 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5596
SacI G_AGCT'C
Cuts at: 2476 2476
Size: 5596
SalI G'TCGA_C
Cuts at: 1936 2938 1936
Size: 1002 4594
SapI GCTCTTCn'nnn_
Cuts at: 2192 2192
Size: 5596
SbfI CC_TGCA'GG
Cuts at: 2948 2948
Size: 5596
ScaI AGT'ACT
Cuts at: 3844 5556 3844
Size: 1712 3884
SfcI C'TryA_G
Cuts at: 826 1045 2944 4079 4760 4951 826
Size: 219 1899 1135 681 191 1471
Fragments arranged by size:
1899 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5596
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5596
SmaI CCC'GGG
Cuts at: 1872 2923 1872
Size: 1051 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3700 4601 4863 820
Size: 197 926 1757 901 262 1553
Fragments arranged by size:
1757 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3387 493
Size: 2894 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5596
SphI G_CATG'C
Cuts at: 1132 2954 1132
Size: 1822 3774
SspI AAT'ATT
Cuts at: 5 52 2425 3520 5
Size: 47 2373 1095 2081
Fragments arranged by size:
2373 2081 1095 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5596
StyI C'CwwG_G
Cuts at: 513 1065 3035 513
Size: 552 1970 3074
Fragments arranged by size:
3074 1970 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2029 2254 3623 3640 3793 3978 4423
Size: 1255 449 225 1369 17 153 185 445
Cuts at: 4423 325
Size: 1498
Fragments arranged by size:
1498 1369 1255 449 445 225 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3842 5554 96
Size: 274 80 33 51 1443 1865 1712 138
Fragments arranged by size:
1865 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5556 5588 5556
Size: 32 5564
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5596
XbaI T'CTAG_A
Cuts at: 2932 2932
Size: 5596
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2177 2335 2177
Size: 158 5438
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5596
XmaI C'CCGG_G
Cuts at: 1870 2921 1870
Size: 1051 4545
XmnI GAAnn'nnTTC
Cuts at: 2796 3725 2796
Size: 929 4667
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3404 276
Size: 53 83 186 936 1870 2468
Fragments arranged by size:
2468 1870 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI SacI SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PpuMI PspXI PsrI PvuII
RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI