Restriction map for
pFN21AE1365
(Circular) (Six-base) MAPSORT of: pFN21AE1365.seq Check: 4693 from: 1 to: 5875
pFN21AE1365 5875 bp
With 182 enzymes: SgfI *
August 16, 2010 14:46 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2675 2675
Size: 5875
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2817 3685 278
Size: 53 83 186 936 1281 868 2468
Fragments arranged by size:
2468 1281 936 868 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2124 3218 1937
Size: 187 1094 4594
Fragments arranged by size:
4594 1094 187
Acc65I G'GTAC_C
Cuts at: 3196 3196
Size: 5875
AclI AA'CG_TT
Cuts at: 1563 4002 4375 1563
Size: 2439 373 3063
Fragments arranged by size:
3063 2439 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3938 5322 5590 1632
Size: 2306 1384 268 1917
Fragments arranged by size:
2306 1917 1384 268
AfeI AGC'GCT
Cuts at: 2054 3077 2054
Size: 1023 4852
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5678
AflIII A'CryG_T
Cuts at: 1203 2082 2862 4774 5751 1203
Size: 879 780 1912 977 1327
Fragments arranged by size:
1912 1327 977 879 780
AgeI A'CCGG_T
Cuts at: 4706 4706
Size: 5875
AhdI GACnn_n'nnGTC
Cuts at: 2737 4604 2737
Size: 1867 4008
AleI CACnn'nnGTG
Cuts at: 1166 2720 1166
Size: 1554 4321
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5875
AlwNI CAG_nnn'CTG
Cuts at: 2107 3618 5190 2107
Size: 1511 1572 2792
Fragments arranged by size:
2792 1572 1511
ApaLI G'TGCA_C
Cuts at: 3932 5088 3932
Size: 1156 4719
ApoI r'AATT_y
Cuts at: 1493 3184 3410 3562 5671 1493
Size: 1691 226 152 2109 1697
Fragments arranged by size:
2109 1697 1691 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4429 160
Size: 872 3397 1606
Fragments arranged by size:
3397 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5875
AvaI C'yCGr_G
Cuts at: 1870 1943 2515 2633 2747 3041 3053 3200 1870
Size: 73 572 118 114 294 12 147 4545
Fragments arranged by size:
4545 572 294 147 118 114 73 12
BamHI G'GATC_C
Cuts at: 2616 3205 3572 2616
Size: 589 367 4919
Fragments arranged by size:
4919 589 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2896 3097 3196 4651 618
Size: 325 217 627 1109 201 99 1455 1842
Fragments arranged by size:
1842 1455 1109 627 325 217 201 99
BanII G_rGCy'C
Cuts at: 1391 2522 2640 2676 3041 3050 3194 1391
Size: 1131 118 36 365 9 144 4072
Fragments arranged by size:
4072 1131 365 144 118 36 9
BbeI G_GCGC'C
Cuts at: 2900 2900
Size: 5875
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2516 2670 928
Size: 971 617 154 4133
Fragments arranged by size:
4133 971 617 154
BbvCI CC'TCA_GC
Cuts at: 2960 2960
Size: 5875
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4066 4100 1903
Size: 34 2129 34 3678
Fragments arranged by size:
3678 2129 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3767 4977 3767
Size: 1210 4665
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5875
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2708 3106 4486
Size: 107 122 71 631 605 1036 398 1380
Cuts at: 4486 136
Size: 1525
Fragments arranged by size:
1525 1380 1036 631 605 398 122 107
71
BglII A'GATC_T
Cuts at: 2142 2325 5870 2142
Size: 183 3545 2147
Fragments arranged by size:
3545 2147 183
BlpI GC'TnA_GC
Cuts at: 3292 3292
Size: 5875
Bme1580I G_kGCm'C
Cuts at: 1790 2282 3102 3936 5092 1790
Size: 492 820 834 1156 2573
Fragments arranged by size:
2573 1156 834 820 492
BmgBI CAC'GTC
Cuts at: 1318 2548 2863 1318
Size: 1230 315 4330
Fragments arranged by size:
4330 1230 315
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3606 4564 442
Size: 416 53 83 399 24 2189 958 1753
Fragments arranged by size:
2189 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5875
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2530 2530
Size: 5875
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4535 1239
Size: 3296 2579
Bpu10I CC'TnA_GC
Cuts at: 2670 2960 2670
Size: 290 5585
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2363 4000 4865 5163 2363
Size: 1637 865 298 3075
Fragments arranged by size:
3075 1637 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4538 882
Size: 617 3039 2219
Fragments arranged by size:
3039 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3666 493
Size: 711 2462 2702
Fragments arranged by size:
2702 2462 711
BsaBI GATnn'nnATC
Cuts at: 5869 5869
Size: 5875
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2814 2897 2970 3010
Size: 53 83 186 936 1281 83 73 40
Cuts at: 3010 3164 3682 4064 4699 275
Size: 154 518 382 635 1451
Fragments arranged by size:
1451 1281 936 635 518 382 186 154
83 83 73 53 40
BsaWI w'CCGG_w
Cuts at: 4308 4706 4980 5127 5538 4308
Size: 398 274 147 411 4645
Fragments arranged by size:
4645 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5650
Fragments arranged by size:
5650 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2336 3071 3074 1186
Size: 1150 735 3 3987
Fragments arranged by size:
3987 1150 735 3
BseYI C'CCAG_C
Cuts at: 2038 2063 2230 2620 5078 5574 2038
Size: 25 167 390 2458 496 2339
Fragments arranged by size:
2458 2339 496 390 167 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5302
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2948 3026 3110 3325 4086 5114
Size: 275 186 947 78 84 215 761 1028
Cuts at: 5114 1540
Size: 2301
Fragments arranged by size:
2301 1028 947 761 275 215 186 84
78
BsiHKAI G_wGCw'C
Cuts at: 3149 3936 4021 5092 3149
Size: 787 85 1071 3932
Fragments arranged by size:
3932 1071 787 85
BsmI GAATG_Cn'
Cuts at: 3386 3479 3386
Size: 93 5782
BsmBI CGTCTCn'nnnn_
Cuts at: 2651 2651
Size: 5875
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2282 2522 2640 2676 3041 3050 3102
Size: 399 492 240 118 36 365 9 52
Cuts at: 3102 3149 3194 3936 4021 5092 1391
Size: 47 45 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 492 399 365 240 118
85 52 47 45 36 9
BspEI T'CCGG_A
Cuts at: 5538 5538
Size: 5875
BspHI T'CATG_A
Cuts at: 2307 3764 5494 2307
Size: 1457 1730 2688
Fragments arranged by size:
2688 1730 1457
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2675 2850 2898 3230 844
Size: 1831 175 48 332 3489
Fragments arranged by size:
3489 1831 332 175 48
BsrBI CCG'CTC
Cuts at: 1394 1823 2577 3052 3064 3762 1394
Size: 429 754 475 12 698 3507
Fragments arranged by size:
3507 754 698 475 429 12
BsrDI GCAATG_nn'
Cuts at: 65 1248 2493 4370 4544 65
Size: 1183 1245 1877 174 1396
Fragments arranged by size:
1877 1396 1245 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2127 2462 3091 4519 4706 1700
Size: 110 317 335 629 1428 187 2869
Fragments arranged by size:
2869 1428 629 335 317 187 110
BsrGI T'GTAC_A
Cuts at: 96 2079 96
Size: 1983 3892
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5875
BssSI C'ACGA_G
Cuts at: 3935 4947 3935
Size: 1012 4863
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5875
BstBI TT'CG_AA
Cuts at: 4679 4679
Size: 5875
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5875
BstYI r'GATC_y
Cuts at: 1972 2142 2325 2616 3205 3572 3957 3974 5426
Size: 170 183 291 589 367 385 17 1452
Cuts at: 5426 5512 5524 5870 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 589 385 367 346 291 183
170 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2388 2538 3104 513
Size: 552 939 384 150 566 3284
Fragments arranged by size:
3284 939 566 552 384 150
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2014 487
Size: 683 56 788 4348
Fragments arranged by size:
4348 788 683 56
BtsI GCAGTG_nn'
Cuts at: 2715 3391 4185 4205 2715
Size: 676 794 20 4385
Fragments arranged by size:
4385 794 676 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5875
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5840
DraI TTT'AAA
Cuts at: 3179 3179
Size: 5875
DraIII CAC_nnn'GTG
Cuts at: 2077 2077
Size: 5875
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4882 809
Size: 507 3566 1802
Fragments arranged by size:
3566 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3023 3107 3322
Size: 54 1425 50 275 18 1193 84 215
Cuts at: 3322 4211 5840 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1193 889 275 215 84 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3023 3107 3322 1537
Size: 275 1211 84 215 4090
Fragments arranged by size:
4090 1211 275 215 84
EarI CTCTTCn'nnn_
Cuts at: 1353 2561 3805 1353
Size: 1208 1244 3423
Fragments arranged by size:
3423 1244 1208
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4462 4836 4982 387
Size: 1064 138 2873 374 146 1280
Fragments arranged by size:
2873 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3938 4535 5322 5590 1239
Size: 393 2306 597 787 268 1524
Fragments arranged by size:
2306 1524 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2747 2963 2747
Size: 216 5659
EcoO109I rG'GnC_Cy
Cuts at: 2104 2104
Size: 5875
EcoRI G'AATT_C
Cuts at: 3184 3562 3184
Size: 378 5497
FspI TGC'GCA
Cuts at: 4381 4381
Size: 5875
HaeII r_GCGC'y
Cuts at: 1678 2056 2134 2900 2959 3079 3117 5022 1678
Size: 378 78 766 59 120 38 1905 2531
Fragments arranged by size:
2531 1905 766 378 120 78 59 38
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2601 2633 2723 2755 4516
Size: 32 135 32 1029 32 90 32 1761
Cuts at: 4516 4548 4590 4622 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1761 1029 135 90 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3219 3465 669
Size: 988 281 1281 246 3079
Fragments arranged by size:
3079 1281 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5875
HpaI GTT'AAC
Cuts at: 3465 3465
Size: 5875
KasI G'GCGC_C
Cuts at: 2896 2896
Size: 5875
KpnI G_GTAC'C
Cuts at: 3200 3200
Size: 5875
MfeI C'AATT_G
Cuts at: 3474 3474
Size: 5875
MluI A'CGCG_T
Cuts at: 5751 5751
Size: 5875
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2091 4989 5173 1305
Size: 786 2898 184 2007
Fragments arranged by size:
2898 2007 786 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4053
Fragments arranged by size:
4053 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2306 2720 3833 4192 4351 518
Size: 648 1140 414 1113 359 159 2042
Fragments arranged by size:
2042 1140 1113 648 414 359 159
MspA1I CmG'CkG
Cuts at: 1606 2257 2334 2944 3106 3135 3291 3968 5116
Size: 651 77 610 162 29 156 677 1148
Cuts at: 5116 5361 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 651 610 245 162 156
77 29
NaeI GCC'GGC
Cuts at: 3093 3093
Size: 5875
NarI GG'CG_CC
Cuts at: 2897 2897
Size: 5875
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4384
Fragments arranged by size:
4384 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5875
NgoMIV G'CCGG_C
Cuts at: 3091 3091
Size: 5875
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5875
NotI GC'GGCC_GC
Cuts at: 3322 3322
Size: 5875
NspI r_CATG'y
Cuts at: 1132 1229 2086 3233 4778 1132
Size: 97 857 1147 1545 2229
Fragments arranged by size:
2229 1545 1147 857 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5875
PciI A'CATG_T
Cuts at: 2082 4774 2082
Size: 2692 3183
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5875
PfoI T'CCnGG_A
Cuts at: 2781 2781
Size: 5875
PmeI GTTT'AAAC
Cuts at: 3179 3179
Size: 5875
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3944 3976 5457 5489 3944
Size: 32 1481 32 4330
Fragments arranged by size:
4330 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2104 2104
Size: 5875
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5875
PsiI TTA'TAA
Cuts at: 3445 3445
Size: 5875
PspXI vC'TCGA_Gb
Cuts at: 2633 2747 3053 2633
Size: 114 306 5455
Fragments arranged by size:
5455 306 114
PstI C_TGCA'G
Cuts at: 830 2300 2481 3003 3140 3227 830
Size: 1470 181 522 137 87 3478
Fragments arranged by size:
3478 1470 522 181 137 87
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5875
SacII CC_GC'GG
Cuts at: 3107 3107
Size: 5875
SalI G'TCGA_C
Cuts at: 1936 3217 1936
Size: 1281 4594
SapI GCTCTTCn'nnn_
Cuts at: 2561 2561
Size: 5875
SbfI CC_TGCA'GG
Cuts at: 3227 3227
Size: 5875
ScaI AGT'ACT
Cuts at: 2294 4123 5835 2294
Size: 1829 1712 2334
Fragments arranged by size:
2334 1829 1712
SfcI C'TryA_G
Cuts at: 826 1045 2296 2477 2999 3136 3223 4358 5039
Size: 219 1251 181 522 137 87 1135 681
Cuts at: 5039 5230 826
Size: 191 1471
Fragments arranged by size:
1471 1251 1135 681 522 219 191 181
137 87
SfoI GGC'GCC
Cuts at: 2898 2898
Size: 5875
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5875
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5875
SmaI CCC'GGG
Cuts at: 1872 3043 3202 1872
Size: 1171 159 4545
Fragments arranged by size:
4545 1171 159
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2378 2633 2747 3053 3979 4880
Size: 197 926 435 255 114 306 926 901
Cuts at: 4880 5142 820
Size: 262 1553
Fragments arranged by size:
1553 926 926 901 435 306 262 255
197 114
SnaBI TAC'GTA
Cuts at: 493 3666 493
Size: 3173 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5875
SphI G_CATG'C
Cuts at: 1132 3233 1132
Size: 2101 3774
SspI AAT'ATT
Cuts at: 5 52 3799 5
Size: 47 3747 2081
Fragments arranged by size:
3747 2081 47
StuI AGG'CCT
Cuts at: 1517 2711 1517
Size: 1194 4681
StyI C'CwwG_G
Cuts at: 513 1065 2004 3314 513
Size: 552 939 1310 3074
Fragments arranged by size:
3074 1310 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3902 3919 4072 4257 4702 325
Size: 1255 2322 17 153 185 445 1498
Fragments arranged by size:
2322 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2079 2292 4121
Size: 274 80 33 51 1443 102 213 1829
Cuts at: 4121 5833 96
Size: 1712 138
Fragments arranged by size:
1829 1712 1443 274 213 138 102 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2777 2809 5835 5867 2777
Size: 32 3026 32 2785
Fragments arranged by size:
3026 2785 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2813 1316
Size: 1497 4378
XbaI T'CTAG_A
Cuts at: 3211 3211
Size: 5875
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2238 2545 3157 2238
Size: 307 612 4956
Fragments arranged by size:
4956 612 307
XhoI C'TCGA_G
Cuts at: 1943 2633 2747 3053 1943
Size: 690 114 306 4765
Fragments arranged by size:
4765 690 306 114
XmaI C'CCGG_G
Cuts at: 1870 3041 3200 1870
Size: 1171 159 4545
Fragments arranged by size:
4545 1171 159
XmnI GAAnn'nnTTC
Cuts at: 2054 4004 2054
Size: 1950 3925
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2815 3683 276
Size: 53 83 186 936 1281 868 2468
Fragments arranged by size:
2468 1281 936 868 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspXI PstI PvuI SacII SalI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BfrBI BsiWI BstXI
BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI NruI
NsiI PacI PmlI PspOMI PsrI PvuII RsrII SacI
SanDI SexAI SfiI SrfI SwaI