Restriction map for
pFN21AE1363
(Circular) (Six-base) MAPSORT of: pFN21AE1363.seq Check: 8119 from: 1 to: 6460
pFN21AE1363 6460 bp
With 182 enzymes: SgfI *
August 16, 2010 14:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4270 278
Size: 53 83 186 936 2734 2468
Fragments arranged by size:
2734 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3803 1937
Size: 1866 4594
Acc65I G'GTAC_C
Cuts at: 3781 3781
Size: 6460
AclI AA'CG_TT
Cuts at: 1563 4587 4960 1563
Size: 3024 373 3063
Fragments arranged by size:
3063 3024 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2252 4523 5907 6175 1632
Size: 620 2271 1384 268 1917
Fragments arranged by size:
2271 1917 1384 620 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6263
AflIII A'CryG_T
Cuts at: 1203 2357 3131 5359 6336 1203
Size: 1154 774 2228 977 1327
Fragments arranged by size:
2228 1327 1154 977 774
AgeI A'CCGG_T
Cuts at: 3067 5291 3067
Size: 2224 4236
AhdI GACnn_n'nnGTC
Cuts at: 5189 5189
Size: 6460
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6460
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6460
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2839 2871 2839
Size: 32 6428
AlwNI CAG_nnn'CTG
Cuts at: 3671 3736 4203 5775 3671
Size: 65 467 1572 4356
Fragments arranged by size:
4356 1572 467 65
ApaLI G'TGCA_C
Cuts at: 2583 4517 5673 2583
Size: 1934 1156 3370
Fragments arranged by size:
3370 1934 1156
ApoI r'AATT_y
Cuts at: 1493 2249 2848 3168 3705 3769 3995 4147 6256
Size: 756 599 320 537 64 226 152 2109
Cuts at: 6256 1493
Size: 1697
Fragments arranged by size:
2109 1697 756 599 537 320 226 152
64
AseI AT'TA_AT
Cuts at: 160 1032 2514 5014 160
Size: 872 1482 2500 1606
Fragments arranged by size:
2500 1606 1482 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6460
AvaI C'yCGr_G
Cuts at: 1870 1943 3785 1870
Size: 73 1842 4545
Fragments arranged by size:
4545 1842 73
BamHI G'GATC_C
Cuts at: 3790 4157 3790
Size: 367 6093
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2008 3099 3532 3781 5236
Size: 325 217 627 221 1091 433 249 1455
Cuts at: 5236 618
Size: 1842
Fragments arranged by size:
1842 1455 1091 627 433 325 249 221
217
BanII G_rGCy'C
Cuts at: 1391 3779 1391
Size: 2388 4072
BbeI G_GCGC'C
Cuts at: 2012 2012
Size: 6460
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5489
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4651 4685 1903
Size: 34 2714 34 3678
Fragments arranged by size:
3678 2714 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2313 3155 4352 5562 2313
Size: 842 1197 1210 3211
Fragments arranged by size:
3211 1210 1197 842
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6460
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5071 136
Size: 107 122 71 631 605 3399 1525
Fragments arranged by size:
3399 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6455 6455
Size: 6460
BlpI GC'TnA_GC
Cuts at: 3877 3877
Size: 6460
Bme1580I G_kGCm'C
Cuts at: 1790 2434 2587 3104 4521 5677 1790
Size: 644 153 517 1417 1156 2573
Fragments arranged by size:
2573 1417 1156 644 517 153
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6460
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2361 4191 5149
Size: 416 53 83 399 24 944 1830 958
Cuts at: 5149 442
Size: 1753
Fragments arranged by size:
1830 1753 958 944 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2724 1056
Size: 1668 4792
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3713 5120 1239
Size: 2474 1407 2579
Fragments arranged by size:
2579 2474 1407
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4585 5450 5748 4585
Size: 865 298 5297
Fragments arranged by size:
5297 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5123 882
Size: 617 3624 2219
Fragments arranged by size:
3624 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4251 493
Size: 711 3047 2702
Fragments arranged by size:
3047 2702 711
BsaBI GATnn'nnATC
Cuts at: 6454 6454
Size: 6460
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2009 4267 4649 5284
Size: 53 83 186 936 476 2258 382 635
Cuts at: 5284 275
Size: 1451
Fragments arranged by size:
2258 1451 936 635 476 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3067 4893 5291 5565 5712 6123 3067
Size: 1826 398 274 147 411 3404
Fragments arranged by size:
3404 1826 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2163 2193 3121 3151 1178
Size: 30 165 30 760 30 928 30 4487
Fragments arranged by size:
4487 928 760 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6460
BseYI C'CCAG_C
Cuts at: 2315 3349 5663 6159 2315
Size: 1034 2314 496 2616
Fragments arranged by size:
2616 2314 1034 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2198 1163
Size: 573 462 5425
Fragments arranged by size:
5425 573 462
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3910 4671 5699 1540
Size: 275 186 1909 761 1028 2301
Fragments arranged by size:
2301 1909 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2587 3139 3557 4521 4606 5677 2587
Size: 552 418 964 85 1071 3370
Fragments arranged by size:
3370 1071 964 552 418 85
BsmI GAATG_Cn'
Cuts at: 3971 4064 3971
Size: 93 6367
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2434 2587 3104 3139 3557 3779 4521
Size: 399 644 153 517 35 418 222 742
Cuts at: 4521 4606 5677 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 644 517 418 399 222
153 85 35
BspEI T'CCGG_A
Cuts at: 6123 6123
Size: 6460
BspHI T'CATG_A
Cuts at: 4349 6079 4349
Size: 1730 4730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3223 3815 844
Size: 2379 592 3489
Fragments arranged by size:
3489 2379 592
BsrBI CCG'CTC
Cuts at: 1394 1823 2023 4347 1394
Size: 429 200 2324 3507
Fragments arranged by size:
3507 2324 429 200
BsrDI GCAATG_nn'
Cuts at: 65 1248 2173 2443 2778 4955 5129 65
Size: 1183 925 270 335 2177 174 1396
Fragments arranged by size:
2177 1396 1183 925 335 270 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3067 5104 5291 1700
Size: 110 1257 2037 187 2869
Fragments arranged by size:
2869 2037 1257 187 110
BsrGI T'GTAC_A
Cuts at: 96 2052 96
Size: 1956 4504
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6460
BssSI C'ACGA_G
Cuts at: 4520 5532 4520
Size: 1012 5448
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6460
BstBI TT'CG_AA
Cuts at: 5264 5264
Size: 6460
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6460
BstYI r'GATC_y
Cuts at: 1972 3790 4157 4542 4559 6011 6097 6109 6455
Size: 1818 367 385 17 1452 86 12 346
Cuts at: 6455 1972
Size: 1977
Fragments arranged by size:
1977 1818 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4969
Fragments arranged by size:
4969 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5721
Fragments arranged by size:
5721 683 56
BtsI GCAGTG_nn'
Cuts at: 3976 4770 4790 3976
Size: 794 20 5646
Fragments arranged by size:
5646 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6460
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6425
DraI TTT'AAA
Cuts at: 3764 3764
Size: 6460
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5467 809
Size: 507 4151 1802
Fragments arranged by size:
4151 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3907 4796 6425
Size: 54 1425 50 275 18 2077 889 1629
Cuts at: 6425 8
Size: 43
Fragments arranged by size:
2077 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3907 1537
Size: 275 2095 4090
Fragments arranged by size:
4090 2095 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3387 4390 1353
Size: 2034 1003 3423
Fragments arranged by size:
3423 2034 1003
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5047 5421 5567 387
Size: 1064 138 3458 374 146 1280
Fragments arranged by size:
3458 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2252 3713 4523 5120 5907 6175 1239
Size: 393 620 1461 810 597 787 268 1524
Fragments arranged by size:
1524 1461 810 787 620 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2231 3728 2231
Size: 1497 4963
EcoO109I rG'GnC_Cy
Cuts at: 2148 3592 2148
Size: 1444 5016
EcoRI G'AATT_C
Cuts at: 2249 2848 3769 4147 2249
Size: 599 921 378 4562
Fragments arranged by size:
4562 921 599 378
FspI TGC'GCA
Cuts at: 4966 4966
Size: 6460
HaeII r_GCGC'y
Cuts at: 1678 2012 5607 1678
Size: 334 3595 2531
Fragments arranged by size:
3595 2531 334
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3197 3229 5101 5133 5175
Size: 32 135 32 1625 32 1872 32 42
Cuts at: 5175 5207 1373
Size: 32 2626
Fragments arranged by size:
2626 1872 1625 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2443 2734 3804 4050 669
Size: 988 281 505 291 1070 246 3079
Fragments arranged by size:
3079 1070 988 505 291 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6460
HpaI GTT'AAC
Cuts at: 2443 4050 2443
Size: 1607 4853
KasI G'GCGC_C
Cuts at: 2008 2008
Size: 6460
KpnI G_GTAC'C
Cuts at: 3785 3785
Size: 6460
MfeI C'AATT_G
Cuts at: 2484 4059 2484
Size: 1575 4885
MluI A'CGCG_T
Cuts at: 6336 6336
Size: 6460
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3678 5574 5758 1305
Size: 2373 1896 184 2007
Fragments arranged by size:
2373 2007 1896 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4638
Fragments arranged by size:
4638 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3201 4418 4777 4936 518
Size: 648 2035 1217 359 159 2042
Fragments arranged by size:
2042 2035 1217 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2315 3876 4553 5701 5946 1606
Size: 709 1561 677 1148 245 2120
Fragments arranged by size:
2120 1561 1148 709 677 245
NarI GG'CG_CC
Cuts at: 2009 2009
Size: 6460
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4969
Fragments arranged by size:
4969 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6460
NheI G'CTAG_C
Cuts at: 1052 2720 1052
Size: 1668 4792
NotI GC'GGCC_GC
Cuts at: 3907 3907
Size: 6460
NspI r_CATG'y
Cuts at: 1132 1229 2361 3135 3818 5363 1132
Size: 97 1132 774 683 1545 2229
Fragments arranged by size:
2229 1545 1132 774 683 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6460
PciI A'CATG_T
Cuts at: 2357 3131 5359 2357
Size: 774 2228 3458
Fragments arranged by size:
3458 2228 774
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2822 3736 1108
Size: 1714 914 3832
Fragments arranged by size:
3832 1714 914
PfoI T'CCnGG_A
Cuts at: 2569 3729 2569
Size: 1160 5300
PmeI GTTT'AAAC
Cuts at: 3764 3764
Size: 6460
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2935 2967 4529 4561 6042 6074 2935
Size: 32 1562 32 1481 32 3321
Fragments arranged by size:
3321 1562 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2148 3592 2148
Size: 1444 5016
PshAI GACnn'nnGTC
Cuts at: 1613 3519 1613
Size: 1906 4554
PsiI TTA'TAA
Cuts at: 4030 4030
Size: 6460
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2754 2786 2754
Size: 32 6428
PstI C_TGCA'G
Cuts at: 830 3812 830
Size: 2982 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6460
PvuII CAG'CTG
Cuts at: 2315 2315
Size: 6460
SalI G'TCGA_C
Cuts at: 1936 3802 1936
Size: 1866 4594
SanDI GG'GwC_CC
Cuts at: 3592 3592
Size: 6460
SbfI CC_TGCA'GG
Cuts at: 3812 3812
Size: 6460
ScaI AGT'ACT
Cuts at: 4708 6420 4708
Size: 1712 4748
SfcI C'TryA_G
Cuts at: 826 1045 2802 2968 3808 4943 5624 5815 826
Size: 219 1757 166 840 1135 681 191 1471
Fragments arranged by size:
1757 1471 1135 840 681 219 191 166
SfoI GGC'GCC
Cuts at: 2010 2010
Size: 6460
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6460
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6460
SmaI CCC'GGG
Cuts at: 1872 3787 1872
Size: 1915 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4564 5465 5727 820
Size: 197 926 2621 901 262 1553
Fragments arranged by size:
2621 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4251 493
Size: 3758 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6460
SphI G_CATG'C
Cuts at: 1132 3818 1132
Size: 2686 3774
SspI AAT'ATT
Cuts at: 5 52 2185 3264 4384 5
Size: 47 2133 1079 1120 2081
Fragments arranged by size:
2133 2081 1120 1079 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6460
StyI C'CwwG_G
Cuts at: 513 1065 2004 2099 3899 513
Size: 552 939 95 1800 3074
Fragments arranged by size:
3074 1800 939 552 95
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2272 4487 4504 4657 4842 5287 325
Size: 1255 692 2215 17 153 185 445 1498
Fragments arranged by size:
2215 1498 1255 692 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2052 4706 6418
Size: 274 80 33 51 1443 75 2654 1712
Cuts at: 6418 96
Size: 138
Fragments arranged by size:
2654 1712 1443 274 138 80 75 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2080 2112 6420 6452 2080
Size: 32 4308 32 2088
Fragments arranged by size:
4308 2088 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6460
XbaI T'CTAG_A
Cuts at: 3796 3796
Size: 6460
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6460
XmaI C'CCGG_G
Cuts at: 1870 3785 1870
Size: 1915 4545
XmnI GAAnn'nnTTC
Cuts at: 2369 4589 2369
Size: 2220 4240
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4268 276
Size: 53 83 186 936 2734 2468
Fragments arranged by size:
2734 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PsrI PstI PvuI PvuII SalI SanDI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI
NsiI PacI PmlI PspOMI PspXI RsrII SacI SacII
SapI SexAI SfiI SrfI SwaI XcmI