Restriction map for
pFN21AE1362
(Circular) (Six-base) MAPSORT of: pFN21AE1362.seq Check: 1003 from: 1 to: 5641
pFN21AE1362 5641 bp
With 182 enzymes: SgfI *
August 16, 2010 14:46 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2605 2605
Size: 5641
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3451 278
Size: 53 83 186 936 1915 2468
Fragments arranged by size:
2468 1915 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2984 1937
Size: 1047 4594
Acc65I G'GTAC_C
Cuts at: 2962 2962
Size: 5641
AclI AA'CG_TT
Cuts at: 1563 3768 4141 1563
Size: 2205 373 3063
Fragments arranged by size:
3063 2205 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2210 2510 2672 3704 5088 5356 1632
Size: 578 300 162 1032 1384 268 1917
Fragments arranged by size:
1917 1384 1032 578 300 268 162
AfeI AGC'GCT
Cuts at: 2405 2405
Size: 5641
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5444
AflIII A'CryG_T
Cuts at: 1203 4540 5517 1203
Size: 3337 977 1327
Fragments arranged by size:
3337 1327 977
AgeI A'CCGG_T
Cuts at: 4472 4472
Size: 5641
AhdI GACnn_n'nnGTC
Cuts at: 4370 4370
Size: 5641
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5641
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5641
AlwNI CAG_nnn'CTG
Cuts at: 2188 2809 2857 3384 4956 2188
Size: 621 48 527 1572 2873
Fragments arranged by size:
2873 1572 621 527 48
ApaI G_GGCC'C
Cuts at: 2509 2509
Size: 5641
ApaLI G'TGCA_C
Cuts at: 3698 4854 3698
Size: 1156 4485
ApoI r'AATT_y
Cuts at: 1493 2950 3176 3328 5437 1493
Size: 1457 226 152 2109 1697
Fragments arranged by size:
2109 1697 1457 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4195 160
Size: 872 3163 1606
Fragments arranged by size:
3163 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5641
AvaI C'yCGr_G
Cuts at: 1870 1943 2500 2508 2870 2966 1870
Size: 73 557 8 362 96 4545
Fragments arranged by size:
4545 557 362 96 73 8
BamHI G'GATC_C
Cuts at: 2971 3338 2971
Size: 367 5274
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2338 2366 2554 2962 4417
Size: 325 217 627 551 28 188 408 1455
Cuts at: 4417 618
Size: 1842
Fragments arranged by size:
1842 1455 627 551 408 325 217 188
28
BanII G_rGCy'C
Cuts at: 1391 2137 2171 2509 2886 2960 1391
Size: 746 34 338 377 74 4072
Fragments arranged by size:
4072 746 377 338 74 34
BbeI G_GCGC'C
Cuts at: 2370 2370
Size: 5641
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4670
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3832 3866 1903
Size: 34 1895 34 3678
Fragments arranged by size:
3678 1895 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3533 4743 3533
Size: 1210 4431
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5641
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2440 4252 136
Size: 107 122 71 631 605 768 1812 1525
Fragments arranged by size:
1812 1525 768 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5636 5636
Size: 5641
BlpI GC'TnA_GC
Cuts at: 2463 2645 3058 2463
Size: 182 413 5046
Fragments arranged by size:
5046 413 182
Bme1580I G_kGCm'C
Cuts at: 1790 2028 2509 2559 3702 4858 1790
Size: 238 481 50 1143 1156 2573
Fragments arranged by size:
2573 1156 1143 481 238 50
BmgBI CAC'GTC
Cuts at: 1318 2176 1318
Size: 858 4783
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3372 4330 442
Size: 416 53 83 399 24 1955 958 1753
Fragments arranged by size:
1955 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5641
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2372 2489 4301 1239
Size: 1133 117 1812 2579
Fragments arranged by size:
2579 1812 1133 117
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3766 4631 4929 3766
Size: 865 298 4478
Fragments arranged by size:
4478 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2690 4304 882
Size: 617 1191 1614 2219
Fragments arranged by size:
2219 1614 1191 617
BsaAI yAC'GTr
Cuts at: 493 1204 3432 493
Size: 711 2228 2702
Fragments arranged by size:
2702 2228 711
BsaBI GATnn'nnATC
Cuts at: 5635 5635
Size: 5641
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2367 3448 3830 4465
Size: 53 83 186 936 834 1081 382 635
Cuts at: 4465 275
Size: 1451
Fragments arranged by size:
1451 1081 936 834 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4074 4472 4746 4893 5304 4074
Size: 398 274 147 411 4411
Fragments arranged by size:
4411 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5416
Fragments arranged by size:
5416 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2320 2414 2888 1186
Size: 1134 94 474 3939
Fragments arranged by size:
3939 1134 474 94
BseYI C'CCAG_C
Cuts at: 2012 2109 2328 2427 2908 4844 5340 2012
Size: 97 219 99 481 1936 496 2313
Fragments arranged by size:
2313 1936 496 481 219 99 97
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2243 2843 1163
Size: 573 507 600 3961
Fragments arranged by size:
3961 600 573 507
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2319 2440 2642 3091 3852 4880
Size: 275 186 318 121 202 449 761 1028
Cuts at: 4880 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 449 318 275 202 186
121
BsiHKAI G_wGCw'C
Cuts at: 2886 3702 3787 4858 2886
Size: 816 85 1071 3669
Fragments arranged by size:
3669 1071 816 85
BsmI GAATG_Cn'
Cuts at: 3152 3245 3152
Size: 93 5548
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2028 2137 2171 2509 2559 2886 2960
Size: 399 238 109 34 338 50 327 74
Cuts at: 2960 3702 3787 4858 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 338 327 238 109
85 74 50 34
BspEI T'CCGG_A
Cuts at: 5304 5304
Size: 5641
BspHI T'CATG_A
Cuts at: 3530 5260 3530
Size: 1730 3911
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2605 2996 844
Size: 1761 391 3489
Fragments arranged by size:
3489 1761 391
BsrBI CCG'CTC
Cuts at: 1394 1823 2160 2374 3528 1394
Size: 429 337 214 1154 3507
Fragments arranged by size:
3507 1154 429 337 214
BsrDI GCAATG_nn'
Cuts at: 65 1248 4136 4310 65
Size: 1183 2888 174 1396
Fragments arranged by size:
2888 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2363 2439 4285 4472 1700
Size: 110 553 76 1846 187 2869
Fragments arranged by size:
2869 1846 553 187 110 76
BsrGI T'GTAC_A
Cuts at: 96 2127 96
Size: 2031 3610
BssHII G'CGCG_C
Cuts at: 1925 2290 2359 2672 1925
Size: 365 69 313 4894
Fragments arranged by size:
4894 365 313 69
BssSI C'ACGA_G
Cuts at: 3701 4713 3701
Size: 1012 4629
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2338 794
Size: 1544 4097
BstBI TT'CG_AA
Cuts at: 4445 4445
Size: 5641
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5641
BstYI r'GATC_y
Cuts at: 1972 2193 2971 3338 3723 3740 5192 5278 5290
Size: 221 778 367 385 17 1452 86 12
Cuts at: 5290 5636 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 778 385 367 346 221 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4150
Fragments arranged by size:
4150 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4902
Fragments arranged by size:
4902 683 56
BtsI GCAGTG_nn'
Cuts at: 2130 2711 3157 3951 3971 2130
Size: 581 446 794 20 3800
Fragments arranged by size:
3800 794 581 446 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5641
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5606
DraI TTT'AAA
Cuts at: 2945 2945
Size: 5641
DraIII CAC_nnn'GTG
Cuts at: 2338 2338
Size: 5641
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2081 2525 4648 809
Size: 507 765 444 2123 1802
Fragments arranged by size:
2123 1802 765 507 444
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2254 2316 2639
Size: 54 1425 50 275 18 424 62 323
Cuts at: 2639 2937 3088 3977 5606 8
Size: 298 151 889 1629 43
Fragments arranged by size:
1629 1425 889 424 323 298 275 151
62 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2316 2639 3088 1537
Size: 275 504 323 449 4090
Fragments arranged by size:
4090 504 449 323 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2025 3571 1353
Size: 672 1546 3423
Fragments arranged by size:
3423 1546 672
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2408 4228 4602 4748 387
Size: 1064 138 819 1820 374 146 1280
Fragments arranged by size:
1820 1280 1064 819 374 146 138
EcoICRI GAG'CTC
Cuts at: 2884 2884
Size: 5641
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2210 2372 2489 2510 2672 3704 4301
Size: 393 578 162 117 21 162 1032 597
Cuts at: 4301 5088 5356 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1032 787 597 578 393 268 162
162 117 21
EcoNI CCTnn'n_nnAGG
Cuts at: 2861 2897 2861
Size: 36 5605
EcoO109I rG'GnC_Cy
Cuts at: 2505 2740 2505
Size: 235 5406
EcoRI G'AATT_C
Cuts at: 2950 3328 2950
Size: 378 5263
FspI TGC'GCA
Cuts at: 4147 4147
Size: 5641
HaeII r_GCGC'y
Cuts at: 1678 2288 2370 2407 2710 2735 2750 2818 2857
Size: 610 82 37 303 25 15 68 39
Cuts at: 2857 4788 1678
Size: 1931 2531
Fragments arranged by size:
2531 1931 610 303 82 68 39 37
25 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4282 4314 4356 4388 1373
Size: 32 135 32 2710 32 42 32 2626
Fragments arranged by size:
2710 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2985 3231 669
Size: 988 281 1047 246 3079
Fragments arranged by size:
3079 1047 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5641
HpaI GTT'AAC
Cuts at: 3231 3231
Size: 5641
KasI G'GCGC_C
Cuts at: 2366 2366
Size: 5641
KpnI G_GTAC'C
Cuts at: 2966 2966
Size: 5641
MfeI C'AATT_G
Cuts at: 3240 3240
Size: 5641
MluI A'CGCG_T
Cuts at: 5517 5517
Size: 5641
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2070 2547 4755 4939 1305
Size: 765 477 2208 184 2007
Fragments arranged by size:
2208 2007 765 477 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3819
Fragments arranged by size:
3819 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3599 3958 4117 518
Size: 648 2433 359 159 2042
Fragments arranged by size:
2433 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2012 2215 2227 2344 2644 2806 3057 3734
Size: 406 203 12 117 300 162 251 677
Cuts at: 3734 4882 5127 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 406 300 251 245 203
162 117 12
NaeI GCC'GGC
Cuts at: 2365 2365
Size: 5641
NarI GG'CG_CC
Cuts at: 2367 2367
Size: 5641
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4150
Fragments arranged by size:
4150 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5641
NgoMIV G'CCGG_C
Cuts at: 2363 2363
Size: 5641
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5641
NotI GC'GGCC_GC
Cuts at: 2316 3088 2316
Size: 772 4869
NspI r_CATG'y
Cuts at: 1132 1229 2999 4544 1132
Size: 97 1770 1545 2229
Fragments arranged by size:
2229 1770 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5641
PciI A'CATG_T
Cuts at: 4540 4540
Size: 5641
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5641
PfoI T'CCnGG_A
Cuts at: 2445 2445
Size: 5641
PmeI GTTT'AAAC
Cuts at: 2945 2945
Size: 5641
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3710 3742 5223 5255 3710
Size: 32 1481 32 4096
Fragments arranged by size:
4096 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2740 2740
Size: 5641
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5641
PsiI TTA'TAA
Cuts at: 3211 3211
Size: 5641
PspOMI G'GGCC_C
Cuts at: 2505 2505
Size: 5641
PspXI vC'TCGA_Gb
Cuts at: 2870 2870
Size: 5641
PstI C_TGCA'G
Cuts at: 830 2142 2184 2304 2718 2853 2993 830
Size: 1312 42 120 414 135 140 3478
Fragments arranged by size:
3478 1312 414 140 135 120 42
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5641
PvuII CAG'CTG
Cuts at: 2012 2215 2227 2806 2012
Size: 203 12 579 4847
Fragments arranged by size:
4847 579 203 12
SacI G_AGCT'C
Cuts at: 2886 2886
Size: 5641
SalI G'TCGA_C
Cuts at: 1936 2983 1936
Size: 1047 4594
SanDI GG'GwC_CC
Cuts at: 2740 2740
Size: 5641
SbfI CC_TGCA'GG
Cuts at: 2993 2993
Size: 5641
ScaI AGT'ACT
Cuts at: 3889 5601 3889
Size: 1712 3929
SfcI C'TryA_G
Cuts at: 826 1045 2138 2180 2300 2628 2714 2849 2989
Size: 219 1093 42 120 328 86 135 140
Cuts at: 2989 4124 4805 4996 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 1093 681 328 219 191 140
135 120 86 42
SfoI GGC'GCC
Cuts at: 2368 2368
Size: 5641
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5641
SgrAI Cr'CCGG_yG
Cuts at: 1700 2363 1700
Size: 663 4978
SmaI CCC'GGG
Cuts at: 1872 2510 2968 1872
Size: 638 458 4545
Fragments arranged by size:
4545 638 458
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2870 3745 4646 4908 820
Size: 197 926 927 875 901 262 1553
Fragments arranged by size:
1553 927 926 901 875 262 197
SnaBI TAC'GTA
Cuts at: 493 3432 493
Size: 2939 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5641
SphI G_CATG'C
Cuts at: 1132 2999 1132
Size: 1867 3774
SspI AAT'ATT
Cuts at: 5 52 3565 5
Size: 47 3513 2081
Fragments arranged by size:
3513 2081 47
StuI AGG'CCT
Cuts at: 1517 2798 1517
Size: 1281 4360
StyI C'CwwG_G
Cuts at: 513 1065 2004 2148 3080 513
Size: 552 939 144 932 3074
Fragments arranged by size:
3074 939 932 552 144
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2026 2933 3668 3685 3838 4023 4468
Size: 1255 446 907 735 17 153 185 445
Cuts at: 4468 325
Size: 1498
Fragments arranged by size:
1498 1255 907 735 446 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2127 3887 5599
Size: 274 80 33 51 1443 150 1760 1712
Cuts at: 5599 96
Size: 138
Fragments arranged by size:
1760 1712 1443 274 150 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5601 5633 5601
Size: 32 5609
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5641
XbaI T'CTAG_A
Cuts at: 2977 2977
Size: 5641
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2932 2932
Size: 5641
XhoI C'TCGA_G
Cuts at: 1943 2870 1943
Size: 927 4714
XmaI C'CCGG_G
Cuts at: 1870 2508 2966 1870
Size: 638 458 4545
Fragments arranged by size:
4545 638 458
XmnI GAAnn'nnTTC
Cuts at: 3770 3770
Size: 5641
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3449 276
Size: 53 83 186 936 1915 2468
Fragments arranged by size:
2468 1915 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII SacI SalI SanDI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I
BsiWI BsmBI BstXI BstZ17I Bsu36I EcoRV FalI FseI
FspAI NruI NsiI PacI PmlI PsrI RsrII SacII
SapI SexAI SfiI SrfI SwaI