Restriction map for
pFN21AE1360
(Circular) (Six-base) MAPSORT of: pFN21AE1360.seq Check: 8653 from: 1 to: 5422
pFN21AE1360 5422 bp
With 182 enzymes: SgfI *
August 16, 2010 14:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3232 278
Size: 53 83 186 936 1696 2468
Fragments arranged by size:
2468 1696 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2765 1937
Size: 828 4594
Acc65I G'GTAC_C
Cuts at: 2743 2743
Size: 5422
AclI AA'CG_TT
Cuts at: 1563 3549 3922 1563
Size: 1986 373 3063
Fragments arranged by size:
3063 1986 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2047 3485 4869 5137 1632
Size: 415 1438 1384 268 1917
Fragments arranged by size:
1917 1438 1384 415 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5225
AflIII A'CryG_T
Cuts at: 1203 4321 5298 1203
Size: 3118 977 1327
Fragments arranged by size:
3118 1327 977
AgeI A'CCGG_T
Cuts at: 4253 4253
Size: 5422
AhdI GACnn_n'nnGTC
Cuts at: 4151 4151
Size: 5422
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5422
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5422
AlwNI CAG_nnn'CTG
Cuts at: 3165 4737 3165
Size: 1572 3850
ApaI G_GGCC'C
Cuts at: 2191 2191
Size: 5422
ApaLI G'TGCA_C
Cuts at: 3479 4635 3479
Size: 1156 4266
ApoI r'AATT_y
Cuts at: 1493 2093 2731 2957 3109 5218 1493
Size: 600 638 226 152 2109 1697
Fragments arranged by size:
2109 1697 638 600 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3976 160
Size: 872 2944 1606
Fragments arranged by size:
2944 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5422
AvaI C'yCGr_G
Cuts at: 1870 1943 2747 1870
Size: 73 804 4545
Fragments arranged by size:
4545 804 73
BamHI G'GATC_C
Cuts at: 2376 2752 3119 2376
Size: 376 367 4679
Fragments arranged by size:
4679 376 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2743 4198 618
Size: 325 217 627 956 1455 1842
Fragments arranged by size:
1842 1455 956 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2191 2610 2741 1391
Size: 800 419 131 4072
Fragments arranged by size:
4072 800 419 131
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2053 928
Size: 971 154 4297
Fragments arranged by size:
4297 971 154
BbvCI CC'TCA_GC
Cuts at: 2088 2088
Size: 5422
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3613 3647 1903
Size: 34 1676 34 3678
Fragments arranged by size:
3678 1676 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3314 4524 3314
Size: 1210 4212
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5422
BfrBI ATG'CAT
Cuts at: 2358 2358
Size: 5422
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4033 136
Size: 107 122 71 631 605 2361 1525
Fragments arranged by size:
2361 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5417 5417
Size: 5422
BlpI GC'TnA_GC
Cuts at: 2646 2839 2646
Size: 193 5229
Bme1580I G_kGCm'C
Cuts at: 1790 2191 3483 4639 1790
Size: 401 1292 1156 2573
Fragments arranged by size:
2573 1292 1156 401
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5422
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3153 4111 442
Size: 416 53 83 399 24 1736 958 1753
Fragments arranged by size:
1753 1736 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5422
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2465 2696 4082 1239
Size: 1226 231 1386 2579
Fragments arranged by size:
2579 1386 1226 231
Bpu10I CC'TnA_GC
Cuts at: 2030 2088 2609 2030
Size: 58 521 4843
Fragments arranged by size:
4843 521 58
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3547 4412 4710 3547
Size: 865 298 4259
Fragments arranged by size:
4259 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4085 882
Size: 617 2586 2219
Fragments arranged by size:
2586 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3213 493
Size: 711 2009 2702
Fragments arranged by size:
2702 2009 711
BsaBI GATnn'nnATC
Cuts at: 5416 5416
Size: 5422
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3229 3611 4246 275
Size: 53 83 186 936 1696 382 635 1451
Fragments arranged by size:
1696 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3855 4253 4527 4674 5085 3855
Size: 398 274 147 411 4192
Fragments arranged by size:
4192 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5197
Fragments arranged by size:
5197 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5422
BseYI C'CCAG_C
Cuts at: 4625 5121 4625
Size: 496 4926
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4849
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2872 3633 4661 1540
Size: 275 186 871 761 1028 2301
Fragments arranged by size:
2301 1028 871 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2610 3483 3568 4639 2610
Size: 873 85 1071 3393
Fragments arranged by size:
3393 1071 873 85
BsmI GAATG_Cn'
Cuts at: 2358 2461 2933 3026 2358
Size: 103 472 93 4754
Fragments arranged by size:
4754 472 103 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2191 2610 2741 3483 3568 4639 1391
Size: 399 401 419 131 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 419 401 399 131 85
BspEI T'CCGG_A
Cuts at: 5085 5085
Size: 5422
BspHI T'CATG_A
Cuts at: 3311 5041 3311
Size: 1730 3692
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2777 844
Size: 1933 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2034 3309 1394
Size: 429 211 1275 3507
Fragments arranged by size:
3507 1275 429 211
BsrDI GCAATG_nn'
Cuts at: 65 1248 3917 4091 65
Size: 1183 2669 174 1396
Fragments arranged by size:
2669 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4066 4253 1700
Size: 110 2256 187 2869
Fragments arranged by size:
2869 2256 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5422
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5422
BssSI C'ACGA_G
Cuts at: 3482 4494 3482
Size: 1012 4410
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5422
BstBI TT'CG_AA
Cuts at: 4226 4226
Size: 5422
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5422
BstXI CCAn_nnnn'nTGG
Cuts at: 2337 2623 2337
Size: 286 5136
BstYI r'GATC_y
Cuts at: 1972 2376 2752 3119 3504 3521 4973 5059 5071
Size: 404 376 367 385 17 1452 86 12
Cuts at: 5071 5417 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 404 385 376 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2630 2630
Size: 5422
BtgI C'CryG_G
Cuts at: 513 1065 2004 2616 513
Size: 552 939 612 3319
Fragments arranged by size:
3319 939 612 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4683
Fragments arranged by size:
4683 683 56
BtsI GCAGTG_nn'
Cuts at: 2938 3732 3752 2938
Size: 794 20 4608
Fragments arranged by size:
4608 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5422
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 1999 2034 529
Size: 35 1435 35 3917
Fragments arranged by size:
3917 1435 35 35
DraI TTT'AAA
Cuts at: 2726 2726
Size: 5422
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4429 809
Size: 507 3113 1802
Fragments arranged by size:
3113 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2529 2869
Size: 54 1425 50 275 18 177 522 340
Cuts at: 2869 3758 5387 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 522 340 275 177 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2869 1537
Size: 275 1057 4090
Fragments arranged by size:
4090 1057 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3352 1353
Size: 1999 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4009 4383 4529 387
Size: 1064 138 2420 374 146 1280
Fragments arranged by size:
2420 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2608 2608
Size: 5422
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2047 2465 2696 3485 4082 4869 5137
Size: 393 415 418 231 789 597 787 268
Cuts at: 5137 1239
Size: 1524
Fragments arranged by size:
1524 789 787 597 418 415 393 268
231
EcoO109I rG'GnC_Cy
Cuts at: 2188 2634 2188
Size: 446 4976
EcoRI G'AATT_C
Cuts at: 2093 2731 3109 2093
Size: 638 378 4406
Fragments arranged by size:
4406 638 378
FspI TGC'GCA
Cuts at: 3928 3928
Size: 5422
HaeII r_GCGC'y
Cuts at: 1678 4569 1678
Size: 2891 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4063 4095 4137 4169 1373
Size: 32 135 32 2491 32 42 32 2626
Fragments arranged by size:
2626 2491 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2689 2766 3012 669
Size: 988 281 751 77 246 3079
Fragments arranged by size:
3079 988 751 281 246 77
HindIII A'AGCT_T
Cuts at: 748 2478 748
Size: 1730 3692
HpaI GTT'AAC
Cuts at: 3012 3012
Size: 5422
KpnI G_GTAC'C
Cuts at: 2747 2747
Size: 5422
MfeI C'AATT_G
Cuts at: 3021 3021
Size: 5422
MluI A'CGCG_T
Cuts at: 5298 5298
Size: 5422
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2404 4536 4720 1305
Size: 1099 2132 184 2007
Fragments arranged by size:
2132 2007 1099 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 2531 10
Size: 54 1425 343 177 522 2901
Fragments arranged by size:
2901 1425 522 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2363 3380 3739 3898 518
Size: 648 1197 1017 359 159 2042
Fragments arranged by size:
2042 1197 1017 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2838 3515 4663 4908 1606
Size: 1232 677 1148 245 2120
Fragments arranged by size:
2120 1232 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2616 513
Size: 552 939 612 3319
Fragments arranged by size:
3319 939 612 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5422
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5422
NotI GC'GGCC_GC
Cuts at: 2869 2869
Size: 5422
NsiI A_TGCA'T
Cuts at: 2360 2360
Size: 5422
NspI r_CATG'y
Cuts at: 1132 1229 2780 4325 1132
Size: 97 1551 1545 2229
Fragments arranged by size:
2229 1551 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2288 1348
Size: 940 4482
PciI A'CATG_T
Cuts at: 4321 4321
Size: 5422
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5422
PfoI T'CCnGG_A
Cuts at: 2079 2430 2079
Size: 351 5071
PmeI GTTT'AAAC
Cuts at: 2726 2726
Size: 5422
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3491 3523 5004 5036 3491
Size: 32 1481 32 3877
Fragments arranged by size:
3877 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5422
PsiI TTA'TAA
Cuts at: 2992 2992
Size: 5422
PspOMI G'GGCC_C
Cuts at: 2187 2187
Size: 5422
PstI C_TGCA'G
Cuts at: 830 2774 830
Size: 1944 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5422
SacI G_AGCT'C
Cuts at: 2610 2610
Size: 5422
SalI G'TCGA_C
Cuts at: 1936 2764 1936
Size: 828 4594
SbfI CC_TGCA'GG
Cuts at: 2774 2774
Size: 5422
ScaI AGT'ACT
Cuts at: 2504 3670 5382 2504
Size: 1166 1712 2544
Fragments arranged by size:
2544 1712 1166
SfcI C'TryA_G
Cuts at: 826 1045 2101 2770 3905 4586 4777 826
Size: 219 1056 669 1135 681 191 1471
Fragments arranged by size:
1471 1135 1056 681 669 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5422
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5422
SmaI CCC'GGG
Cuts at: 1872 2749 1872
Size: 877 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3526 4427 4689 820
Size: 197 926 1583 901 262 1553
Fragments arranged by size:
1583 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3213 493
Size: 2720 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5422
SphI G_CATG'C
Cuts at: 1132 2780 1132
Size: 1648 3774
SspI AAT'ATT
Cuts at: 5 52 3346 5
Size: 47 3294 2081
Fragments arranged by size:
3294 2081 47
StuI AGG'CCT
Cuts at: 1517 2537 1517
Size: 1020 4402
StyI C'CwwG_G
Cuts at: 513 1065 2004 2223 2532 2541 2571 2616 2861
Size: 552 939 219 309 9 30 45 245
Cuts at: 2861 513
Size: 3074
Fragments arranged by size:
3074 939 552 309 245 219 45 30
9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2236 3449 3466 3619 3804 4249 325
Size: 1255 656 1213 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1213 656 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2502 3668 5380
Size: 274 80 33 51 1443 525 1166 1712
Cuts at: 5380 96
Size: 138
Fragments arranged by size:
1712 1443 1166 525 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2184 2216 5382 5414 2184
Size: 32 3166 32 2192
Fragments arranged by size:
3166 2192 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5422
XbaI T'CTAG_A
Cuts at: 2758 2758
Size: 5422
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5422
XmaI C'CCGG_G
Cuts at: 1870 2747 1870
Size: 877 4545
XmnI GAAnn'nnTTC
Cuts at: 2075 3551 2075
Size: 1476 3946
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3230 276
Size: 53 83 186 936 1696 2468
Fragments arranged by size:
2468 1696 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PspOMI PstI PvuI SacI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BplI
BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI
PpuMI PspXI PsrI PvuII RsrII SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI