Restriction map for
pFN21AE1359
(Circular) (Six-base) MAPSORT of: pFN21AE1359.seq Check: 1879 from: 1 to: 5935
pFN21AE1359 5935 bp
With 182 enzymes: SgfI *
August 16, 2010 14:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3745 278
Size: 53 83 186 936 2209 2468
Fragments arranged by size:
2468 2209 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2760 2896 3278 1937
Size: 823 136 382 4594
Fragments arranged by size:
4594 823 382 136
Acc65I G'GTAC_C
Cuts at: 3256 3256
Size: 5935
AclI AA'CG_TT
Cuts at: 1563 4062 4435 1563
Size: 2499 373 3063
Fragments arranged by size:
3063 2499 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3034 3062 3998 5382 5650 1632
Size: 1402 28 936 1384 268 1917
Fragments arranged by size:
1917 1402 1384 936 268 28
AflII C'TTAA_G
Cuts at: 820 1017 2386 2714 820
Size: 197 1369 328 4041
Fragments arranged by size:
4041 1369 328 197
AflIII A'CryG_T
Cuts at: 1203 4834 5811 1203
Size: 3631 977 1327
Fragments arranged by size:
3631 1327 977
AgeI A'CCGG_T
Cuts at: 2526 4766 2526
Size: 2240 3695
AhdI GACnn_n'nnGTC
Cuts at: 4664 4664
Size: 5935
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5935
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2233 2658 1184
Size: 1049 425 4461
Fragments arranged by size:
4461 1049 425
AlwNI CAG_nnn'CTG
Cuts at: 3678 5250 3678
Size: 1572 4363
ApaLI G'TGCA_C
Cuts at: 3992 5148 3992
Size: 1156 4779
ApoI r'AATT_y
Cuts at: 1493 2679 2882 3244 3470 3622 5731 1493
Size: 1186 203 362 226 152 2109 1697
Fragments arranged by size:
2109 1697 1186 362 226 203 152
AseI AT'TA_AT
Cuts at: 160 1032 2696 4489 160
Size: 872 1664 1793 1606
Fragments arranged by size:
1793 1664 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5935
AvaI C'yCGr_G
Cuts at: 1870 1943 3123 3260 1870
Size: 73 1180 137 4545
Fragments arranged by size:
4545 1180 137 73
BamHI G'GATC_C
Cuts at: 2125 3265 3632 2125
Size: 1140 367 4428
Fragments arranged by size:
4428 1140 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3256 4711 618
Size: 325 217 627 1469 1455 1842
Fragments arranged by size:
1842 1469 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3035 3183 3254 1391
Size: 1644 148 71 4072
Fragments arranged by size:
4072 1644 148 71
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4964
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4126 4160 1903
Size: 34 50 34 2105 34 3678
Fragments arranged by size:
3678 2105 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2823 3827 5037 2823
Size: 1004 1210 3721
Fragments arranged by size:
3721 1210 1004
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5935
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4546 136
Size: 107 122 71 631 605 2874 1525
Fragments arranged by size:
2874 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5930 5930
Size: 5935
BlpI GC'TnA_GC
Cuts at: 3352 3352
Size: 5935
Bme1580I G_kGCm'C
Cuts at: 1790 3996 5152 1790
Size: 2206 1156 2573
Fragments arranged by size:
2573 2206 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5935
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3168 3666 4624
Size: 416 53 83 399 24 1751 498 958
Cuts at: 4624 442
Size: 1753
Fragments arranged by size:
1753 1751 958 498 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5935
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2071 4595 1239
Size: 832 2524 2579
Fragments arranged by size:
2579 2524 832
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2426 2630 4060 4925 5223 2426
Size: 204 1430 865 298 3138
Fragments arranged by size:
3138 1430 865 298 204
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2489 4598 882
Size: 617 990 2109 2219
Fragments arranged by size:
2219 2109 990 617
BsaAI yAC'GTr
Cuts at: 493 1204 3726 493
Size: 711 2522 2702
Fragments arranged by size:
2702 2522 711
BsaBI GATnn'nnATC
Cuts at: 5929 5929
Size: 5935
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3057 3742 4124 4759
Size: 53 83 186 936 1524 685 382 635
Cuts at: 4759 275
Size: 1451
Fragments arranged by size:
1524 1451 936 685 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2526 4368 4766 5040 5187 5598 2526
Size: 1842 398 274 147 411 2863
Fragments arranged by size:
2863 1842 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2204 2234 1178
Size: 30 165 30 801 30 4879
Fragments arranged by size:
4879 801 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3156 3173 1186
Size: 1970 17 3948
Fragments arranged by size:
3948 1970 17
BseYI C'CCAG_C
Cuts at: 2331 2817 5138 5634 2331
Size: 486 2321 496 2632
Fragments arranged by size:
2632 2321 496 486
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2229 1163
Size: 573 493 4869
Fragments arranged by size:
4869 573 493
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3385 4146 5174 1540
Size: 275 186 1384 761 1028 2301
Fragments arranged by size:
2301 1384 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3035 3996 4081 5152 3035
Size: 961 85 1071 3818
Fragments arranged by size:
3818 1071 961 85
BsmI GAATG_Cn'
Cuts at: 3446 3539 3446
Size: 93 5842
BsmBI CGTCTCn'nnnn_
Cuts at: 3017 3017
Size: 5935
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3035 3183 3254 3996 4081 5152 1391
Size: 399 1245 148 71 742 85 1071 2174
Fragments arranged by size:
2174 1245 1071 742 399 148 85 71
BspEI T'CCGG_A
Cuts at: 5598 5598
Size: 5935
BspHI T'CATG_A
Cuts at: 3824 5554 3824
Size: 1730 4205
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2758 3290 844
Size: 1914 532 3489
Fragments arranged by size:
3489 1914 532
BsrBI CCG'CTC
Cuts at: 1394 1823 2026 2041 3201 3822 1394
Size: 429 203 15 1160 621 3507
Fragments arranged by size:
3507 1160 621 429 203 15
BsrDI GCAATG_nn'
Cuts at: 65 1248 4430 4604 65
Size: 1183 3182 174 1396
Fragments arranged by size:
3182 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2526 4579 4766 1700
Size: 110 716 2053 187 2869
Fragments arranged by size:
2869 2053 716 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5935
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5935
BssSI C'ACGA_G
Cuts at: 3995 5007 3995
Size: 1012 4923
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2207 794
Size: 1413 4522
BstBI TT'CG_AA
Cuts at: 4739 4739
Size: 5935
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5935
BstYI r'GATC_y
Cuts at: 1972 2125 2851 3265 3632 4017 4034 5486 5572
Size: 153 726 414 367 385 17 1452 86
Cuts at: 5572 5584 5930 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 726 414 385 367 346 153
86 17 12
BstZ17I GTA'TAC
Cuts at: 2761 2897 2761
Size: 136 5799
BtgI C'CryG_G
Cuts at: 513 1065 2688 3009 513
Size: 552 1623 321 3439
Fragments arranged by size:
3439 1623 552 321
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5196
Fragments arranged by size:
5196 683 56
BtsI GCAGTG_nn'
Cuts at: 3451 4245 4265 3451
Size: 794 20 5121
Fragments arranged by size:
5121 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5935
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5900
DraI TTT'AAA
Cuts at: 3239 3239
Size: 5935
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2743 4942 809
Size: 507 1427 2199 1802
Fragments arranged by size:
2199 1802 1427 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2142 3382 4271
Size: 54 1425 50 275 18 312 1240 889
Cuts at: 4271 5900 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1240 889 312 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3382 1537
Size: 275 1570 4090
Fragments arranged by size:
4090 1570 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2087 2424 3865 1353
Size: 734 337 1441 3423
Fragments arranged by size:
3423 1441 734 337
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4522 4896 5042 387
Size: 1064 138 2933 374 146 1280
Fragments arranged by size:
2933 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3033 3033
Size: 5935
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2071 3034 3062 3998 4595 5382 5650
Size: 393 439 963 28 936 597 787 268
Cuts at: 5650 1239
Size: 1524
Fragments arranged by size:
1524 963 936 787 597 439 393 268
28
EcoO109I rG'GnC_Cy
Cuts at: 2710 2710
Size: 5935
EcoRI G'AATT_C
Cuts at: 3244 3622 3244
Size: 378 5557
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2401 2401
Size: 5935
FspI TGC'GCA
Cuts at: 4441 4441
Size: 5935
HaeII r_GCGC'y
Cuts at: 1678 5082 1678
Size: 3404 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2204 2236 2334 2366 4576
Size: 32 135 32 632 32 98 32 2210
Cuts at: 4576 4608 4650 4682 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2210 632 135 98 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2872 3279 3525 669
Size: 988 281 934 407 246 3079
Fragments arranged by size:
3079 988 934 407 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5935
HpaI GTT'AAC
Cuts at: 3525 3525
Size: 5935
KpnI G_GTAC'C
Cuts at: 3260 3260
Size: 5935
MfeI C'AATT_G
Cuts at: 3534 3534
Size: 5935
MluI A'CGCG_T
Cuts at: 5811 5811
Size: 5935
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2829 5049 5233 1305
Size: 1524 2220 184 2007
Fragments arranged by size:
2220 2007 1524 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2144 10
Size: 54 1425 343 312 3801
Fragments arranged by size:
3801 1425 343 312 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3893 4252 4411 518
Size: 648 2727 359 159 2042
Fragments arranged by size:
2727 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2317 3219 3351 4028 5176 5421 1606
Size: 711 902 132 677 1148 245 2120
Fragments arranged by size:
2120 1148 902 711 677 245 132
NcoI C'CATG_G
Cuts at: 513 1065 2688 3009 513
Size: 552 1623 321 3439
Fragments arranged by size:
3439 1623 552 321
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5935
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5935
NotI GC'GGCC_GC
Cuts at: 3382 3382
Size: 5935
NspI r_CATG'y
Cuts at: 1132 1229 2767 3293 4838 1132
Size: 97 1538 526 1545 2229
Fragments arranged by size:
2229 1545 1538 526 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5935
PciI A'CATG_T
Cuts at: 4834 4834
Size: 5935
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5935
PfoI T'CCnGG_A
Cuts at: 2087 2862 2087
Size: 775 5160
PmeI GTTT'AAAC
Cuts at: 3239 3239
Size: 5935
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4004 4036 5517 5549 4004
Size: 32 1481 32 4390
Fragments arranged by size:
4390 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5935
PsiI TTA'TAA
Cuts at: 3017 3505 3017
Size: 488 5447
PstI C_TGCA'G
Cuts at: 830 2427 3287 830
Size: 1597 860 3478
Fragments arranged by size:
3478 1597 860
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5935
SacI G_AGCT'C
Cuts at: 3035 3035
Size: 5935
SalI G'TCGA_C
Cuts at: 1936 3277 1936
Size: 1341 4594
SbfI CC_TGCA'GG
Cuts at: 3287 3287
Size: 5935
ScaI AGT'ACT
Cuts at: 4183 5895 4183
Size: 1712 4223
SfcI C'TryA_G
Cuts at: 826 1045 2423 3283 4418 5099 5290 826
Size: 219 1378 860 1135 681 191 1471
Fragments arranged by size:
1471 1378 1135 860 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5935
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5935
SmaI CCC'GGG
Cuts at: 1872 3262 1872
Size: 1390 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2386 2405 2609 2714 4039 4940
Size: 197 926 443 19 204 105 1325 901
Cuts at: 4940 5202 820
Size: 262 1553
Fragments arranged by size:
1553 1325 926 901 443 262 204 197
105 19
SnaBI TAC'GTA
Cuts at: 493 3726 493
Size: 3233 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5935
SphI G_CATG'C
Cuts at: 1132 3293 1132
Size: 2161 3774
SspI AAT'ATT
Cuts at: 5 52 3859 5
Size: 47 3807 2081
Fragments arranged by size:
3807 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5935
StyI C'CwwG_G
Cuts at: 513 1065 2186 2688 3009 3374 513
Size: 552 1121 502 321 365 3074
Fragments arranged by size:
3074 1121 552 502 365 321
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3962 3979 4132 4317 4762 325
Size: 1255 2382 17 153 185 445 1498
Fragments arranged by size:
2382 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2323 4181 5893
Size: 274 80 33 51 1443 346 1858 1712
Cuts at: 5893 96
Size: 138
Fragments arranged by size:
1858 1712 1443 346 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5895 5927 5895
Size: 32 5903
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5935
XbaI T'CTAG_A
Cuts at: 3271 3271
Size: 5935
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5935
XmaI C'CCGG_G
Cuts at: 1870 3260 1870
Size: 1390 4545
XmnI GAAnn'nnTTC
Cuts at: 3047 4064 3047
Size: 1017 4918
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3743 276
Size: 53 83 186 936 2209 2468
Fragments arranged by size:
2468 2209 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI
PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BstXI Bsu36I DraIII
EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI