Restriction map for
pFN21AE1357
(Circular) (Six-base) MAPSORT of: pFN21AE1357.seq Check: 2098 from: 1 to: 5161
pFN21AE1357 5161 bp
With 182 enzymes: SgfI *
August 16, 2010 14:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2971 278
Size: 53 83 186 936 1435 2468
Fragments arranged by size:
2468 1435 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2504 1937
Size: 567 4594
Acc65I G'GTAC_C
Cuts at: 2482 2482
Size: 5161
AclI AA'CG_TT
Cuts at: 1563 3288 3661 1563
Size: 1725 373 3063
Fragments arranged by size:
3063 1725 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2154 2205 2264 3224 4608 4876 1632
Size: 522 51 59 960 1384 268 1917
Fragments arranged by size:
1917 1384 960 522 268 59 51
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4964
AflIII A'CryG_T
Cuts at: 1203 4060 5037 1203
Size: 2857 977 1327
Fragments arranged by size:
2857 1327 977
AgeI A'CCGG_T
Cuts at: 3992 3992
Size: 5161
AhdI GACnn_n'nnGTC
Cuts at: 3890 3890
Size: 5161
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5161
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5161
AlwNI CAG_nnn'CTG
Cuts at: 2338 2904 4476 2338
Size: 566 1572 3023
Fragments arranged by size:
3023 1572 566
ApaLI G'TGCA_C
Cuts at: 3218 4374 3218
Size: 1156 4005
ApoI r'AATT_y
Cuts at: 1493 2177 2470 2696 2848 4957 1493
Size: 684 293 226 152 2109 1697
Fragments arranged by size:
2109 1697 684 293 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3715 160
Size: 872 2683 1606
Fragments arranged by size:
2683 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5161
AvaI C'yCGr_G
Cuts at: 1870 1943 2486 1870
Size: 73 543 4545
Fragments arranged by size:
4545 543 73
BamHI G'GATC_C
Cuts at: 2059 2491 2858 2059
Size: 432 367 4362
Fragments arranged by size:
4362 432 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2025 2482 3937 618
Size: 325 217 627 238 457 1455 1842
Fragments arranged by size:
1842 1455 627 457 325 238 217
BanII G_rGCy'C
Cuts at: 1391 2086 2480 1391
Size: 695 394 4072
Fragments arranged by size:
4072 695 394
BbeI G_GCGC'C
Cuts at: 2029 2029
Size: 5161
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2304 928
Size: 971 405 3785
Fragments arranged by size:
3785 971 405
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3352 3386 1903
Size: 34 1415 34 3678
Fragments arranged by size:
3678 1415 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3053 4263 3053
Size: 1210 3951
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5161
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3772 136
Size: 107 122 71 631 605 2100 1525
Fragments arranged by size:
2100 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5156 5156
Size: 5161
BlpI GC'TnA_GC
Cuts at: 2385 2578 2385
Size: 193 4968
Bme1580I G_kGCm'C
Cuts at: 1790 3222 4378 1790
Size: 1432 1156 2573
Fragments arranged by size:
2573 1432 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5161
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2892 3850 442
Size: 416 53 83 399 24 1475 958 1753
Fragments arranged by size:
1753 1475 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5161
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2049 3821 1239
Size: 810 1772 2579
Fragments arranged by size:
2579 1772 810
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3286 4151 4449 3286
Size: 865 298 3998
Fragments arranged by size:
3998 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3824 882
Size: 617 2325 2219
Fragments arranged by size:
2325 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2952 493
Size: 711 1748 2702
Fragments arranged by size:
2702 1748 711
BsaBI GATnn'nnATC
Cuts at: 5155 5155
Size: 5161
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2026 2968 3350 3985
Size: 53 83 186 936 493 942 382 635
Cuts at: 3985 275
Size: 1451
Fragments arranged by size:
1451 942 936 635 493 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3594 3992 4266 4413 4824 3594
Size: 398 274 147 411 3931
Fragments arranged by size:
3931 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4936
Fragments arranged by size:
4936 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5161
BseYI C'CCAG_C
Cuts at: 4364 4860 4364
Size: 496 4665
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4588
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2611 3372 4400 1540
Size: 275 186 610 761 1028 2301
Fragments arranged by size:
2301 1028 761 610 275 186
BsiHKAI G_wGCw'C
Cuts at: 3222 3307 4378 3222
Size: 85 1071 4005
Fragments arranged by size:
4005 1071 85
BsmI GAATG_Cn'
Cuts at: 2672 2765 2672
Size: 93 5068
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2086 2480 3222 3307 4378 1391
Size: 399 296 394 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 394 296 85
BspEI T'CCGG_A
Cuts at: 4824 4824
Size: 5161
BspHI T'CATG_A
Cuts at: 3050 4780 3050
Size: 1730 3431
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2516 844
Size: 1672 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3048 1394
Size: 429 1225 3507
Fragments arranged by size:
3507 1225 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3656 3830 65
Size: 1183 2408 174 1396
Fragments arranged by size:
2408 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3805 3992 1700
Size: 110 1995 187 2869
Fragments arranged by size:
2869 1995 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5161
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5161
BssSI C'ACGA_G
Cuts at: 3221 4233 3221
Size: 1012 4149
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5161
BstBI TT'CG_AA
Cuts at: 3965 3965
Size: 5161
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5161
BstYI r'GATC_y
Cuts at: 1972 2059 2491 2858 3243 3260 4712 4798 4810
Size: 87 432 367 385 17 1452 86 12
Cuts at: 4810 5156 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 432 385 367 346 87 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4609
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4422
Fragments arranged by size:
4422 683 56
BtsI GCAGTG_nn'
Cuts at: 2677 3471 3491 2677
Size: 794 20 4347
Fragments arranged by size:
4347 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5161
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5126
DraI TTT'AAA
Cuts at: 2164 2465 2164
Size: 301 4860
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4168 809
Size: 507 2852 1802
Fragments arranged by size:
2852 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2608 3497 5126
Size: 54 1425 50 275 18 778 889 1629
Cuts at: 5126 8
Size: 43
Fragments arranged by size:
1629 1425 889 778 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2608 1537
Size: 275 796 4090
Fragments arranged by size:
4090 796 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3091 1353
Size: 1738 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3748 4122 4268 387
Size: 1064 138 2159 374 146 1280
Fragments arranged by size:
2159 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2049 2154 2205 2264 3224 3821 4608
Size: 393 417 105 51 59 960 597 787
Cuts at: 4608 4876 1239
Size: 268 1524
Fragments arranged by size:
1524 960 787 597 417 393 268 105
59 51
EcoO109I rG'GnC_Cy
Cuts at: 2139 2139
Size: 5161
EcoRI G'AATT_C
Cuts at: 2177 2470 2848 2177
Size: 293 378 4490
Fragments arranged by size:
4490 378 293
FspI TGC'GCA
Cuts at: 3667 3667
Size: 5161
HaeII r_GCGC'y
Cuts at: 1678 2029 4308 1678
Size: 351 2279 2531
Fragments arranged by size:
2531 2279 351
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2221 2253 3802 3834 3876
Size: 32 135 32 649 32 1549 32 42
Cuts at: 3876 3908 1373
Size: 32 2626
Fragments arranged by size:
2626 1549 649 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2505 2751 669
Size: 988 281 567 246 3079
Fragments arranged by size:
3079 988 567 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5161
HpaI GTT'AAC
Cuts at: 2751 2751
Size: 5161
KasI G'GCGC_C
Cuts at: 2025 2025
Size: 5161
KpnI G_GTAC'C
Cuts at: 2486 2486
Size: 5161
MfeI C'AATT_G
Cuts at: 2760 2760
Size: 5161
MluI A'CGCG_T
Cuts at: 5037 5037
Size: 5161
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2270 2321 2322 4275 4459 1305
Size: 965 51 1 1953 184 2007
Fragments arranged by size:
2007 1953 965 184 51 1
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3339
Fragments arranged by size:
3339 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2105 2241 3119 3478 3637 518
Size: 648 939 136 878 359 159 2042
Fragments arranged by size:
2042 939 878 648 359 159 136
MspA1I CmG'CkG
Cuts at: 1606 2079 2389 2577 3254 4402 4647 1606
Size: 473 310 188 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 473 310 245 188
NarI GG'CG_CC
Cuts at: 2026 2026
Size: 5161
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4609
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5161
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5161
NotI GC'GGCC_GC
Cuts at: 2608 2608
Size: 5161
NspI r_CATG'y
Cuts at: 1132 1229 2519 4064 1132
Size: 97 1290 1545 2229
Fragments arranged by size:
2229 1545 1290 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5161
PciI A'CATG_T
Cuts at: 4060 4060
Size: 5161
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5161
PmeI GTTT'AAAC
Cuts at: 2465 2465
Size: 5161
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3230 3262 4743 4775 3230
Size: 32 1481 32 3616
Fragments arranged by size:
3616 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2139 2139
Size: 5161
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5161
PsiI TTA'TAA
Cuts at: 2731 2731
Size: 5161
PstI C_TGCA'G
Cuts at: 830 2513 830
Size: 1683 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5161
PvuII CAG'CTG
Cuts at: 2389 2389
Size: 5161
SalI G'TCGA_C
Cuts at: 1936 2503 1936
Size: 567 4594
SanDI GG'GwC_CC
Cuts at: 2139 2139
Size: 5161
SbfI CC_TGCA'GG
Cuts at: 2513 2513
Size: 5161
ScaI AGT'ACT
Cuts at: 3409 5121 3409
Size: 1712 3449
SfcI C'TryA_G
Cuts at: 826 1045 2208 2509 3644 4325 4516 826
Size: 219 1163 301 1135 681 191 1471
Fragments arranged by size:
1471 1163 1135 681 301 219 191
SfoI GGC'GCC
Cuts at: 2027 2027
Size: 5161
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5161
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5161
SmaI CCC'GGG
Cuts at: 1872 2488 1872
Size: 616 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3265 4166 4428 820
Size: 197 926 1322 901 262 1553
Fragments arranged by size:
1553 1322 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2952 493
Size: 2459 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5161
SphI G_CATG'C
Cuts at: 1132 2519 1132
Size: 1387 3774
SspI AAT'ATT
Cuts at: 5 52 3085 5
Size: 47 3033 2081
Fragments arranged by size:
3033 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5161
StyI C'CwwG_G
Cuts at: 513 1065 2600 513
Size: 552 1535 3074
Fragments arranged by size:
3074 1535 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3188 3205 3358 3543 3988 325
Size: 1255 1608 17 153 185 445 1498
Fragments arranged by size:
1608 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3407 5119 96
Size: 274 80 33 51 1443 1430 1712 138
Fragments arranged by size:
1712 1443 1430 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5121 5153 5121
Size: 32 5129
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5161
XbaI T'CTAG_A
Cuts at: 2497 2497
Size: 5161
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5161
XmaI C'CCGG_G
Cuts at: 1870 2486 1870
Size: 616 4545
XmnI GAAnn'nnTTC
Cuts at: 3290 3290
Size: 5161
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2969 276
Size: 53 83 186 936 1435 2468
Fragments arranged by size:
2468 1435 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SalI SanDI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI
NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI
PsrI RsrII SacI SacII SapI SexAI SfiI SrfI
SwaI XcmI