Restriction map for
pFN21AE1355
(Circular) (Six-base) MAPSORT of: pFN21AE1355.seq Check: 3211 from: 1 to: 5551
pFN21AE1355 5551 bp
With 182 enzymes: SgfI *
November 21, 2014 12:14 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2520 2559 3361 278
Size: 53 83 186 936 984 39 802 2468
Fragments arranged by size:
2468 984 936 802 186 83 53 39
AccI GT'mk_AC
Cuts at: 1937 2520 2894 1937
Size: 583 374 4594
Fragments arranged by size:
4594 583 374
Acc65I G'GTAC_C
Cuts at: 2872 2872
Size: 5551
AclI AA'CG_TT
Cuts at: 1563 3678 4051 1563
Size: 2115 373 3063
Fragments arranged by size:
3063 2115 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2093 2867 3614 4998 5266 1632
Size: 461 774 747 1384 268 1917
Fragments arranged by size:
1917 1384 774 747 461 268
AfeI AGC'GCT
Cuts at: 2306 2668 2306
Size: 362 5189
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5354
AflIII A'CryG_T
Cuts at: 1203 4450 5427 1203
Size: 3247 977 1327
Fragments arranged by size:
3247 1327 977
AgeI A'CCGG_T
Cuts at: 4382 4382
Size: 5551
AhdI GACnn_n'nnGTC
Cuts at: 2335 4280 2335
Size: 1945 3606
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5551
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2036 1184
Size: 852 4699
AlwNI CAG_nnn'CTG
Cuts at: 2155 2653 3294 4866 2155
Size: 498 641 1572 2840
Fragments arranged by size:
2840 1572 641 498
ApaLI G'TGCA_C
Cuts at: 3608 4764 3608
Size: 1156 4395
ApoI r'AATT_y
Cuts at: 1493 2860 3086 3238 5347 1493
Size: 1367 226 152 2109 1697
Fragments arranged by size:
2109 1697 1367 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4105 160
Size: 872 3073 1606
Fragments arranged by size:
3073 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5551
AvaI C'yCGr_G
Cuts at: 1870 1943 2479 2876 1870
Size: 73 536 397 4545
Fragments arranged by size:
4545 536 397 73
BamHI G'GATC_C
Cuts at: 2881 3248 2881
Size: 367 5184
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2738 2872 4327 618
Size: 325 217 627 951 134 1455 1842
Fragments arranged by size:
1842 1455 951 627 325 217 134
BanII G_rGCy'C
Cuts at: 1391 2379 2870 1391
Size: 988 491 4072
Fragments arranged by size:
4072 988 491
BbeI G_GCGC'C
Cuts at: 2742 2742
Size: 5551
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4580
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2561 2595 3742 3776 1903
Size: 34 624 34 1147 34 3678
Fragments arranged by size:
3678 1147 624 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3443 4653 3443
Size: 1210 4341
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5551
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4162 136
Size: 107 122 71 631 605 2490 1525
Fragments arranged by size:
2490 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5546 5546
Size: 5551
BlpI GC'TnA_GC
Cuts at: 2968 2968
Size: 5551
Bme1580I G_kGCm'C
Cuts at: 1790 3612 4768 1790
Size: 1822 1156 2573
Fragments arranged by size:
2573 1822 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5551
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2291 2602 3282
Size: 416 53 83 399 24 874 311 680
Cuts at: 3282 4240 442
Size: 958 1753
Fragments arranged by size:
1753 958 874 680 416 399 311 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5551
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4211 1239
Size: 2972 2579
Bpu10I CC'TnA_GC
Cuts at: 2156 2156
Size: 5551
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3676 4541 4839 3676
Size: 865 298 4388
Fragments arranged by size:
4388 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2102 2593 4214 882
Size: 617 603 491 1621 2219
Fragments arranged by size:
2219 1621 617 603 491
BsaAI yAC'GTr
Cuts at: 493 1204 2266 3342 493
Size: 711 1062 1076 2702
Fragments arranged by size:
2702 1076 1062 711
BsaBI GATnn'nnATC
Cuts at: 5545 5545
Size: 5551
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2517 2556 2739 3358
Size: 53 83 186 936 984 39 183 619
Cuts at: 3358 3740 4375 275
Size: 382 635 1451
Fragments arranged by size:
1451 984 936 635 619 382 186 183
83 53 39
BsaWI w'CCGG_w
Cuts at: 3984 4382 4656 4803 5214 3984
Size: 398 274 147 411 4321
Fragments arranged by size:
4321 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5326
Fragments arranged by size:
5326 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5551
BseYI C'CCAG_C
Cuts at: 2023 4754 5250 2023
Size: 2731 496 2324
Fragments arranged by size:
2731 2324 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2168 1163
Size: 573 432 4546
Fragments arranged by size:
4546 573 432
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3001 3762 4790 1540
Size: 275 186 1000 761 1028 2301
Fragments arranged by size:
2301 1028 1000 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3612 3697 4768 3612
Size: 85 1071 4395
Fragments arranged by size:
4395 1071 85
BsmI GAATG_Cn'
Cuts at: 3062 3155 3062
Size: 93 5458
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2379 2870 3612 3697 4768 1391
Size: 399 589 491 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 589 491 399 85
BspEI T'CCGG_A
Cuts at: 5214 5214
Size: 5551
BspHI T'CATG_A
Cuts at: 3440 5170 3440
Size: 1730 3821
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2906 844
Size: 2062 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3438 1394
Size: 429 1615 3507
Fragments arranged by size:
3507 1615 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4046 4220 65
Size: 1183 2798 174 1396
Fragments arranged by size:
2798 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4195 4382 1700
Size: 110 2385 187 2869
Fragments arranged by size:
2869 2385 187 110
BsrGI T'GTAC_A
Cuts at: 96 2319 96
Size: 2223 3328
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5551
BssSI C'ACGA_G
Cuts at: 3611 4623 3611
Size: 1012 4539
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5551
BstBI TT'CG_AA
Cuts at: 4355 4355
Size: 5551
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5551
BstXI CCAn_nnnn'nTGG
Cuts at: 2102 2102
Size: 5551
BstYI r'GATC_y
Cuts at: 1972 2151 2881 3248 3633 3650 5102 5188 5200
Size: 179 730 367 385 17 1452 86 12
Cuts at: 5200 5546 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 730 385 367 346 179 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2211 513
Size: 552 1146 3853
Fragments arranged by size:
3853 1146 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2155 487
Size: 683 56 929 3883
Fragments arranged by size:
3883 929 683 56
BtsI GCAGTG_nn'
Cuts at: 3067 3861 3881 3067
Size: 794 20 4737
Fragments arranged by size:
4737 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5551
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5516
DraI TTT'AAA
Cuts at: 2855 2855
Size: 5551
DraIII CAC_nnn'GTG
Cuts at: 2266 2266
Size: 5551
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2116 4558 809
Size: 507 800 2442 1802
Fragments arranged by size:
2442 1802 800 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2998 3887 5516
Size: 54 1425 50 275 18 1168 889 1629
Cuts at: 5516 8
Size: 43
Fragments arranged by size:
1629 1425 1168 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2998 1537
Size: 275 1186 4090
Fragments arranged by size:
4090 1186 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2766 3481 1353
Size: 1413 715 3423
Fragments arranged by size:
3423 1413 715
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2612 4138 4512 4658 387
Size: 1064 138 1023 1526 374 146 1280
Fragments arranged by size:
1526 1280 1064 1023 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2093 2867 3614 4211 4998 5266 1239
Size: 393 461 774 747 597 787 268 1524
Fragments arranged by size:
1524 787 774 747 597 461 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2054 2180 2237 2704 2054
Size: 126 57 467 4901
Fragments arranged by size:
4901 467 126 57
EcoRI G'AATT_C
Cuts at: 2860 3238 2860
Size: 378 5173
FspI TGC'GCA
Cuts at: 4057 4057
Size: 5551
HaeII r_GCGC'y
Cuts at: 1678 2033 2308 2670 2742 4698 1678
Size: 355 275 362 72 1956 2531
Fragments arranged by size:
2531 1956 362 355 275 72
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2321 2353 2491 2523 4192
Size: 32 135 32 749 32 138 32 1669
Cuts at: 4192 4224 4266 4298 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1669 749 138 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2895 3141 669
Size: 988 281 957 246 3079
Fragments arranged by size:
3079 988 957 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5551
HpaI GTT'AAC
Cuts at: 3141 3141
Size: 5551
KasI G'GCGC_C
Cuts at: 2738 2738
Size: 5551
KpnI G_GTAC'C
Cuts at: 2876 2876
Size: 5551
MfeI C'AATT_G
Cuts at: 3150 3150
Size: 5551
MluI A'CGCG_T
Cuts at: 5427 5427
Size: 5551
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4665 4849 1305
Size: 3360 184 2007
Fragments arranged by size:
3360 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3729
Fragments arranged by size:
3729 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2564 3509 3868 4027 518
Size: 648 1398 945 359 159 2042
Fragments arranged by size:
2042 1398 945 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2967 3644 4792 5037 1606
Size: 1361 677 1148 245 2120
Fragments arranged by size:
2120 1361 1148 677 245
NarI GG'CG_CC
Cuts at: 2739 2739
Size: 5551
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4999
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5551
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5551
NotI GC'GGCC_GC
Cuts at: 2998 2998
Size: 5551
NspI r_CATG'y
Cuts at: 1132 1229 2474 2909 4454 1132
Size: 97 1245 435 1545 2229
Fragments arranged by size:
2229 1545 1245 435 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5551
PciI A'CATG_T
Cuts at: 4450 4450
Size: 5551
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5551
PfoI T'CCnGG_A
Cuts at: 2181 2181
Size: 5551
PmeI GTTT'AAAC
Cuts at: 2855 2855
Size: 5551
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3620 3652 5133 5165 3620
Size: 32 1481 32 4006
Fragments arranged by size:
4006 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5551
PsiI TTA'TAA
Cuts at: 3121 3121
Size: 5551
PstI C_TGCA'G
Cuts at: 830 2058 2591 2903 830
Size: 1228 533 312 3478
Fragments arranged by size:
3478 1228 533 312
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5551
SalI G'TCGA_C
Cuts at: 1936 2893 1936
Size: 957 4594
SapI GCTCTTCn'nnn_
Cuts at: 2766 2766
Size: 5551
SbfI CC_TGCA'GG
Cuts at: 2903 2903
Size: 5551
ScaI AGT'ACT
Cuts at: 3799 5511 3799
Size: 1712 3839
SexAI A'CCwGG_T
Cuts at: 2331 2331
Size: 5551
SfcI C'TryA_G
Cuts at: 826 1045 2054 2587 2899 4034 4715 4906 826
Size: 219 1009 533 312 1135 681 191 1471
Fragments arranged by size:
1471 1135 1009 681 533 312 219 191
SfoI GGC'GCC
Cuts at: 2740 2740
Size: 5551
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5551
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5551
SmaI CCC'GGG
Cuts at: 1872 2481 2878 1872
Size: 609 397 4545
Fragments arranged by size:
4545 609 397
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3655 4556 4818 820
Size: 197 926 1712 901 262 1553
Fragments arranged by size:
1712 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3342 493
Size: 2849 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5551
SphI G_CATG'C
Cuts at: 1132 2474 2909 1132
Size: 1342 435 3774
Fragments arranged by size:
3774 1342 435
SspI AAT'ATT
Cuts at: 5 52 3475 5
Size: 47 3423 2081
Fragments arranged by size:
3423 2081 47
StuI AGG'CCT
Cuts at: 1517 2326 1517
Size: 809 4742
StyI C'CwwG_G
Cuts at: 513 1065 2990 513
Size: 552 1925 3074
Fragments arranged by size:
3074 1925 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2701 3578 3595 3748 3933 4378 325
Size: 1255 1121 877 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1121 877 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2319 3797 5509
Size: 274 80 33 51 1443 342 1478 1712
Cuts at: 5509 96
Size: 138
Fragments arranged by size:
1712 1478 1443 342 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5511 5543 5511
Size: 32 5519
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5551
XbaI T'CTAG_A
Cuts at: 2887 2887
Size: 5551
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2783 2783
Size: 5551
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5551
XmaI C'CCGG_G
Cuts at: 1870 2479 2876 1870
Size: 609 397 4545
Fragments arranged by size:
4545 609 397
XmnI GAAnn'nnTTC
Cuts at: 3680 3680
Size: 5551
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2518 2557 3359 276
Size: 53 83 186 936 984 39 802 2468
Fragments arranged by size:
2468 984 936 802 186 83 53 39
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PstI PvuI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoO109I EcoRV
FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI
PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SfiI SrfI SwaI