Restriction map for
pFN21AE1354
(Circular) (Six-base) MAPSORT of: pFN21AE1354.seq Check: 6912 from: 1 to: 7438
pFN21AE1354 7438 bp
With 182 enzymes: SgfI *
August 16, 2010 14:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2202 3960 2202
Size: 1758 5680
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5248 278
Size: 53 83 186 936 3712 2468
Fragments arranged by size:
3712 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4458 4781 1937
Size: 2521 323 4594
Fragments arranged by size:
4594 2521 323
Acc65I G'GTAC_C
Cuts at: 4759 4759
Size: 7438
AclI AA'CG_TT
Cuts at: 1563 5565 5938 1563
Size: 4002 373 3063
Fragments arranged by size:
4002 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3332 4094 5501 6885 7153 1632
Size: 1700 762 1407 1384 268 1917
Fragments arranged by size:
1917 1700 1407 1384 762 268
AfeI AGC'GCT
Cuts at: 2349 2349
Size: 7438
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7241
AflIII A'CryG_T
Cuts at: 1203 3663 6337 7314 1203
Size: 2460 2674 977 1327
Fragments arranged by size:
2674 2460 1327 977
AgeI A'CCGG_T
Cuts at: 6269 6269
Size: 7438
AhdI GACnn_n'nnGTC
Cuts at: 3534 4507 4699 6167 3534
Size: 973 192 1468 4805
Fragments arranged by size:
4805 1468 973 192
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7438
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7438
AlwNI CAG_nnn'CTG
Cuts at: 2998 3889 5181 6753 2998
Size: 891 1292 1572 3683
Fragments arranged by size:
3683 1572 1292 891
ApaI G_GGCC'C
Cuts at: 3993 4365 4670 3993
Size: 372 305 6761
Fragments arranged by size:
6761 372 305
ApaLI G'TGCA_C
Cuts at: 2321 3026 5495 6651 2321
Size: 705 2469 1156 3108
Fragments arranged by size:
3108 2469 1156 705
ApoI r'AATT_y
Cuts at: 1493 4747 4973 5125 7234 1493
Size: 3254 226 152 2109 1697
Fragments arranged by size:
3254 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5992 160
Size: 872 4960 1606
Fragments arranged by size:
4960 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7438
AvaI C'yCGr_G
Cuts at: 1870 1943 4364 4763 1870
Size: 73 2421 399 4545
Fragments arranged by size:
4545 2421 399 73
BamHI G'GATC_C
Cuts at: 2928 3996 4768 5135 2928
Size: 1068 772 367 5231
Fragments arranged by size:
5231 1068 772 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2520 3486 3892 4654 4685
Size: 325 217 627 733 966 406 762 31
Cuts at: 4685 4759 6214 618
Size: 74 1455 1842
Fragments arranged by size:
1842 1455 966 762 733 627 406 325
217 74 31
BanII G_rGCy'C
Cuts at: 1391 2250 2511 3444 3993 4365 4670 4757 1391
Size: 859 261 933 549 372 305 87 4072
Fragments arranged by size:
4072 933 859 549 372 305 261 87
BbeI G_GCGC'C
Cuts at: 4658 4658
Size: 7438
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6467
BbvCI CC'TCA_GC
Cuts at: 2648 3101 4596 2648
Size: 453 1495 5490
Fragments arranged by size:
5490 1495 453
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2197 2231 3377 3411 5629 5663 1903
Size: 34 260 34 1146 34 2218 34 3678
Fragments arranged by size:
3678 2218 1146 260 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5330 6540 5330
Size: 1210 6228
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7438
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3767 3974 4652
Size: 107 122 71 631 605 2095 207 678
Cuts at: 4652 6049 136
Size: 1397 1525
Fragments arranged by size:
2095 1525 1397 678 631 605 207 122
107 71
BglII A'GATC_T
Cuts at: 3495 7433 3495
Size: 3938 3500
BlpI GC'TnA_GC
Cuts at: 4064 4855 4064
Size: 791 6647
Bme1580I G_kGCm'C
Cuts at: 1790 2325 2967 3030 3993 4365 4670 4683 4690
Size: 535 642 63 963 372 305 13 7
Cuts at: 4690 5499 6655 1790
Size: 809 1156 2573
Fragments arranged by size:
2573 1156 963 809 642 535 372 305
63 13 7
BmgBI CAC'GTC
Cuts at: 1318 4405 1318
Size: 3087 4351
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2125 5169 6127
Size: 416 53 83 399 24 708 3044 958
Cuts at: 6127 442
Size: 1753
Fragments arranged by size:
3044 1753 958 708 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7438
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2264 2264
Size: 7438
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2288 2942 2972 3020 3083 3248 3572 3593
Size: 1049 654 30 48 63 165 324 21
Cuts at: 3593 3641 3938 4073 4145 6098 1239
Size: 48 297 135 72 1953 2579
Fragments arranged by size:
2579 1953 1049 654 324 297 165 135
72 63 48 48 30 21
Bpu10I CC'TnA_GC
Cuts at: 2295 2648 3101 4596 2295
Size: 353 453 1495 5137
Fragments arranged by size:
5137 1495 453 353
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2234 5563 6428 6726 2234
Size: 3329 865 298 2946
Fragments arranged by size:
3329 2946 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2389 2483 3148 4511 6101 882
Size: 617 890 94 665 1363 1590 2219
Fragments arranged by size:
2219 1590 1363 890 665 617 94
BsaAI yAC'GTr
Cuts at: 493 1204 5229 493
Size: 711 4025 2702
Fragments arranged by size:
4025 2702 711
BsaBI GATnn'nnATC
Cuts at: 7432 7432
Size: 7438
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2373 3513 3741 4278
Size: 53 83 186 936 840 1140 228 537
Cuts at: 4278 4319 4655 4674 5245 5627 6262 275
Size: 41 336 19 571 382 635 1451
Fragments arranged by size:
1451 1140 936 840 635 571 537 382
336 228 186 83 53 41 19
BsaWI w'CCGG_w
Cuts at: 2841 5871 6269 6543 6690 7101 2841
Size: 3030 398 274 147 411 3178
Fragments arranged by size:
3178 3030 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3449 3479 1178
Size: 30 165 30 2046 30 5137
Fragments arranged by size:
5137 2046 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3212 3701 3935 4028 4382 4613 1186
Size: 2026 489 234 93 354 231 4011
Fragments arranged by size:
4011 2026 489 354 234 231 93
BseYI C'CCAG_C
Cuts at: 2048 4209 4357 6641 7137 2048
Size: 2161 148 2284 496 2349
Fragments arranged by size:
2349 2284 2161 496 148
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2579 3065 3575 3902 1163
Size: 573 843 486 510 327 4699
Fragments arranged by size:
4699 843 573 510 486 327
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4417 4888 5649 6677 1540
Size: 275 186 2416 471 761 1028 2301
Fragments arranged by size:
2416 2301 1028 761 471 275 186
BsiHKAI G_wGCw'C
Cuts at: 2325 2883 3030 3444 3951 4197 5499 5584 6655
Size: 558 147 414 507 246 1302 85 1071
Cuts at: 6655 2325
Size: 3108
Fragments arranged by size:
3108 1302 1071 558 507 414 246 147
85
BsmI GAATG_Cn'
Cuts at: 4949 5042 4949
Size: 93 7345
BsmBI CGTCTCn'nnnn_
Cuts at: 2235 3323 2235
Size: 1088 6350
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2250 2325 2511 2883 2967 3030 3444
Size: 399 460 75 186 372 84 63 414
Cuts at: 3444 3951 3993 4197 4365 4670 4683 4690 4757
Size: 507 42 204 168 305 13 7 67
Cuts at: 4757 5499 5584 6655 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 507 460 414 399 372
305 204 186 168 85 84 75 67
63 42 13 7
BspEI T'CCGG_A
Cuts at: 7101 7101
Size: 7438
BspHI T'CATG_A
Cuts at: 4446 5327 7057 4446
Size: 881 1730 4827
Fragments arranged by size:
4827 1730 881
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2136 2202 3960 4128 4793 844
Size: 1292 66 1758 168 665 3489
Fragments arranged by size:
3489 1758 1292 665 168 66
BsrBI CCG'CTC
Cuts at: 1394 1823 2287 2305 3232 5325 1394
Size: 429 464 18 927 2093 3507
Fragments arranged by size:
3507 2093 927 464 429 18
BsrDI GCAATG_nn'
Cuts at: 65 1248 2068 3429 4230 5933 6107 65
Size: 1183 820 1361 801 1703 174 1396
Fragments arranged by size:
1703 1396 1361 1183 820 801 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2613 3018 3108 4416 6082 6269 1700
Size: 110 803 405 90 1308 1666 187 2869
Fragments arranged by size:
2869 1666 1308 803 405 187 110 90
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7438
BssHII G'CGCG_C
Cuts at: 1925 3610 1925
Size: 1685 5753
BssSI C'ACGA_G
Cuts at: 3278 3371 5498 6510 3278
Size: 93 2127 1012 4206
Fragments arranged by size:
4206 2127 1012 93
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3892 794
Size: 3098 4340
BstBI TT'CG_AA
Cuts at: 2688 6242 2688
Size: 3554 3884
BstEII G'GTnAC_C
Cuts at: 1184 2289 1184
Size: 1105 6333
BstXI CCAn_nnnn'nTGG
Cuts at: 2725 3985 2725
Size: 1260 6178
BstYI r'GATC_y
Cuts at: 1972 2844 2928 3216 3495 3996 4263 4768 5135
Size: 872 84 288 279 501 267 505 367
Cuts at: 5135 5520 5537 6989 7075 7087 7433 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 872 505 501 385 367 346
288 279 267 86 84 17 12
Bsu36I CC'TnA_GG
Cuts at: 2456 2456
Size: 7438
BtgI C'CryG_G
Cuts at: 513 1065 2004 2481 3645 4188 4710 513
Size: 552 939 477 1164 543 522 3241
Fragments arranged by size:
3241 1164 939 552 543 522 477
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4534 4627 487
Size: 683 56 3308 93 3298
Fragments arranged by size:
3308 3298 683 93 56
BtsI GCAGTG_nn'
Cuts at: 4954 5748 5768 4954
Size: 794 20 6624
Fragments arranged by size:
6624 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7438
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7403
DraI TTT'AAA
Cuts at: 4742 4742
Size: 7438
DraIII CAC_nnn'GTG
Cuts at: 3486 3486
Size: 7438
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2663 2785 6445 809
Size: 507 1347 122 3660 1802
Fragments arranged by size:
3660 1802 1347 507 122
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2496 2907 3333
Size: 54 1425 50 275 18 666 411 426
Cuts at: 3333 3768 4885 5774 7403 8
Size: 435 1117 889 1629 43
Fragments arranged by size:
1629 1425 1117 889 666 435 426 411
275 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4885 1537
Size: 275 3073 4090
Fragments arranged by size:
4090 3073 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2394 2481 5368 1353
Size: 1041 87 2887 3423
Fragments arranged by size:
3423 2887 1041 87
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6025 6399 6545 387
Size: 1064 138 4436 374 146 1280
Fragments arranged by size:
4436 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3442 3442
Size: 7438
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2288 2942 2972 3020 3083 3248 3332
Size: 393 656 654 30 48 63 165 84
Cuts at: 3332 3572 3593 3641 3938 4073 4094 4145 5501
Size: 240 21 48 297 135 21 51 1356
Cuts at: 5501 6098 6885 7153 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1356 787 656 654 597 393 297
268 240 165 135 84 63 51 48
48 30 21 21
EcoNI CCTnn'n_nnAGG
Cuts at: 2198 2888 3917 2198
Size: 690 1029 5719
Fragments arranged by size:
5719 1029 690
EcoO109I rG'GnC_Cy
Cuts at: 2125 3652 3697 3989 3990 4047 4153 4361 4666
Size: 1527 45 292 1 57 106 208 305
Cuts at: 4666 4667 2125
Size: 1 4896
Fragments arranged by size:
4896 1527 305 292 208 106 57 45
1 1
EcoRI G'AATT_C
Cuts at: 4747 5125 4747
Size: 378 7060
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2528 2528
Size: 7438
FspI TGC'GCA
Cuts at: 3399 5944 3399
Size: 2545 4893
HaeII r_GCGC'y
Cuts at: 1678 2070 2351 3645 4358 4658 6585 1678
Size: 392 281 1294 713 300 1927 2531
Fragments arranged by size:
2531 1927 1294 713 392 300 281
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2261 2293 3520 3552 4493
Size: 32 135 32 689 32 1227 32 941
Cuts at: 4493 4525 4685 4717 6079 6111 6153 6185 1373
Size: 32 160 32 1362 32 42 32 2626
Fragments arranged by size:
2626 1362 1227 941 689 160 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2866 3478 4459 4782 5028 669
Size: 988 281 928 612 981 323 246 3079
Fragments arranged by size:
3079 988 981 928 612 323 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7438
HpaI GTT'AAC
Cuts at: 5028 5028
Size: 7438
KasI G'GCGC_C
Cuts at: 4654 4654
Size: 7438
KpnI G_GTAC'C
Cuts at: 4763 4763
Size: 7438
MfeI C'AATT_G
Cuts at: 5037 5037
Size: 7438
MluI A'CGCG_T
Cuts at: 7314 7314
Size: 7438
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3966 6552 6736 1305
Size: 2661 2586 184 2007
Fragments arranged by size:
2661 2586 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2909 3770 10
Size: 54 1425 343 1077 861 3678
Fragments arranged by size:
3678 1425 1077 861 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2480 4037 4202 5396 5755 5914 518
Size: 648 1314 1557 165 1194 359 159 2042
Fragments arranged by size:
2042 1557 1314 1194 648 359 165 159
MspA1I CmG'CkG
Cuts at: 1606 2577 3061 3571 3589 3889 4063 4219 4595
Size: 971 484 510 18 300 174 156 376
Cuts at: 4595 4854 5531 6679 6924 1606
Size: 259 677 1148 245 2120
Fragments arranged by size:
2120 1148 971 677 510 484 376 300
259 245 174 156 18
NarI GG'CG_CC
Cuts at: 4655 4655
Size: 7438
NcoI C'CATG_G
Cuts at: 513 1065 2004 3645 4188 513
Size: 552 939 1641 543 3763
Fragments arranged by size:
3763 1641 939 552 543
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7438
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7438
NotI GC'GGCC_GC
Cuts at: 4885 4885
Size: 7438
NspI r_CATG'y
Cuts at: 1132 1229 3667 4796 6341 1132
Size: 97 2438 1129 1545 2229
Fragments arranged by size:
2438 2229 1545 1129 97
PasI CC'CwG_GG
Cuts at: 1348 2431 1348
Size: 1083 6355
PciI A'CATG_T
Cuts at: 3663 6337 3663
Size: 2674 4764
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2946 1108
Size: 1838 5600
PfoI T'CCnGG_A
Cuts at: 2265 2469 2919 2979 3657 3915 4146 2265
Size: 204 450 60 678 258 231 5557
Fragments arranged by size:
5557 678 450 258 231 204 60
PmeI GTTT'AAAC
Cuts at: 4742 4742
Size: 7438
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4418 4450 5507 5539 7020 7052 4418
Size: 32 1057 32 1481 32 4804
Fragments arranged by size:
4804 1481 1057 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2125 3652 4047 2125
Size: 1527 395 5516
Fragments arranged by size:
5516 1527 395
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7438
PsiI TTA'TAA
Cuts at: 5008 5008
Size: 7438
PspOMI G'GGCC_C
Cuts at: 3989 4361 4666 3989
Size: 372 305 6761
Fragments arranged by size:
6761 372 305
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2018 2050 2018
Size: 32 7406
PstI C_TGCA'G
Cuts at: 830 3087 4790 830
Size: 2257 1703 3478
Fragments arranged by size:
3478 2257 1703
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7438
PvuII CAG'CTG
Cuts at: 2577 3061 3571 3589 3889 4063 4219 4595 2577
Size: 484 510 18 300 174 156 376 5420
Fragments arranged by size:
5420 510 484 376 300 174 156 18
SacI G_AGCT'C
Cuts at: 3444 3444
Size: 7438
SalI G'TCGA_C
Cuts at: 1936 4457 4780 1936
Size: 2521 323 4594
Fragments arranged by size:
4594 2521 323
SanDI GG'GwC_CC
Cuts at: 4047 4047
Size: 7438
SbfI CC_TGCA'GG
Cuts at: 4790 4790
Size: 7438
ScaI AGT'ACT
Cuts at: 4637 5686 7398 4637
Size: 1049 1712 4677
Fragments arranged by size:
4677 1712 1049
SexAI A'CCwGG_T
Cuts at: 4542 4734 4542
Size: 192 7246
SfcI C'TryA_G
Cuts at: 826 1045 3083 4786 5921 6602 6793 826
Size: 219 2038 1703 1135 681 191 1471
Fragments arranged by size:
2038 1703 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 3767 3767
Size: 7438
SfoI GGC'GCC
Cuts at: 4656 4656
Size: 7438
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7438
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7438
SmaI CCC'GGG
Cuts at: 1872 4765 1872
Size: 2893 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2249 5542 6443 6705 820
Size: 197 926 306 3293 901 262 1553
Fragments arranged by size:
3293 1553 926 901 306 262 197
SnaBI TAC'GTA
Cuts at: 493 5229 493
Size: 4736 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7438
SphI G_CATG'C
Cuts at: 1132 4796 1132
Size: 3664 3774
SspI AAT'ATT
Cuts at: 5 52 5362 5
Size: 47 5310 2081
Fragments arranged by size:
5310 2081 47
StuI AGG'CCT
Cuts at: 1517 3269 3284 3377 4379 1517
Size: 1752 15 93 1002 4576
Fragments arranged by size:
4576 1752 1002 93 15
StyI C'CwwG_G
Cuts at: 513 1065 2004 2499 3039 3645 4188 4281 4877
Size: 552 939 495 540 606 543 93 596
Cuts at: 4877 513
Size: 3074
Fragments arranged by size:
3074 939 606 596 552 543 540 495
93
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5465 5482 5635 5820 6265 325
Size: 1255 3885 17 153 185 445 1498
Fragments arranged by size:
3885 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4635 5684 7396
Size: 274 80 33 51 1443 2658 1049 1712
Cuts at: 7396 96
Size: 138
Fragments arranged by size:
2658 1712 1443 1049 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7398 7430 7398
Size: 32 7406
Tth111I GACn'n_nGTC
Cuts at: 1316 2663 4544 1316
Size: 1347 1881 4210
Fragments arranged by size:
4210 1881 1347
XbaI T'CTAG_A
Cuts at: 4774 4774
Size: 7438
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2917 2947 4186 4249 2917
Size: 30 1239 63 6106
Fragments arranged by size:
6106 1239 63 30
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7438
XmaI C'CCGG_G
Cuts at: 1870 4763 1870
Size: 2893 4545
XmnI GAAnn'nnTTC
Cuts at: 5567 5567
Size: 7438
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5246 276
Size: 53 83 186 936 3712 2468
Fragments arranged by size:
3712 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI
PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I EcoRV
FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI
PspXI RsrII SacII SapI SrfI SwaI