Restriction map for
pFN21AE1353
(Circular) (Six-base) MAPSORT of: pFN21AE1353.seq Check: 4978 from: 1 to: 5326
pFN21AE1353 5326 bp
With 182 enzymes: SgfI *
August 16, 2010 14:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3136 278
Size: 53 83 186 936 1600 2468
Fragments arranged by size:
2468 1600 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2129 2669 1937
Size: 192 540 4594
Fragments arranged by size:
4594 540 192
Acc65I G'GTAC_C
Cuts at: 2319 2647 2319
Size: 328 4998
AclI AA'CG_TT
Cuts at: 1563 3453 3826 1563
Size: 1890 373 3063
Fragments arranged by size:
3063 1890 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3389 4773 5041 1632
Size: 1757 1384 268 1917
Fragments arranged by size:
1917 1757 1384 268
AfeI AGC'GCT
Cuts at: 2234 2234
Size: 5326
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5129
AflIII A'CryG_T
Cuts at: 1203 4225 5202 1203
Size: 3022 977 1327
Fragments arranged by size:
3022 1327 977
AgeI A'CCGG_T
Cuts at: 4157 4157
Size: 5326
AhdI GACnn_n'nnGTC
Cuts at: 4055 4055
Size: 5326
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5326
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5326
AlwNI CAG_nnn'CTG
Cuts at: 3069 4641 3069
Size: 1572 3754
ApaLI G'TGCA_C
Cuts at: 3383 4539 3383
Size: 1156 4170
ApoI r'AATT_y
Cuts at: 1493 2635 2861 3013 5122 1493
Size: 1142 226 152 2109 1697
Fragments arranged by size:
2109 1697 1142 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3880 160
Size: 872 2848 1606
Fragments arranged by size:
2848 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5326
AvaI C'yCGr_G
Cuts at: 1870 1943 2151 2323 2651 1870
Size: 73 208 172 328 4545
Fragments arranged by size:
4545 328 208 172 73
BamHI G'GATC_C
Cuts at: 2656 3023 2656
Size: 367 4959
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2164 2189 2209 2319 2647
Size: 325 217 627 377 25 20 110 328
Cuts at: 2647 4102 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 377 328 325 217 110
25 20
BanII G_rGCy'C
Cuts at: 1391 2012 2184 2645 1391
Size: 621 172 461 4072
Fragments arranged by size:
4072 621 461 172
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4355
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3517 3551 1903
Size: 34 1580 34 3678
Fragments arranged by size:
3678 1580 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3218 4428 3218
Size: 1210 4116
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5326
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2281 3937 136
Size: 107 122 71 631 605 609 1656 1525
Fragments arranged by size:
1656 1525 631 609 605 122 107 71
BglII A'GATC_T
Cuts at: 5321 5321
Size: 5326
BlpI GC'TnA_GC
Cuts at: 2743 2743
Size: 5326
Bme1580I G_kGCm'C
Cuts at: 1790 2169 2192 2214 2277 3387 4543 1790
Size: 379 23 22 63 1110 1156 2573
Fragments arranged by size:
2573 1156 1110 379 63 23 22
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5326
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2289 3057 4015
Size: 416 53 83 399 24 872 768 958
Cuts at: 4015 442
Size: 1753
Fragments arranged by size:
1753 958 872 768 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5326
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2285 2496 3986 1239
Size: 1046 211 1490 2579
Fragments arranged by size:
2579 1490 1046 211
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3451 4316 4614 3451
Size: 865 298 4163
Fragments arranged by size:
4163 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3989 882
Size: 617 2490 2219
Fragments arranged by size:
2490 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3117 493
Size: 711 1913 2702
Fragments arranged by size:
2702 1913 711
BsaBI GATnn'nnATC
Cuts at: 2303 5320 2303
Size: 3017 2309
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2592 3133 3515 4150
Size: 53 83 186 936 1059 541 382 635
Cuts at: 4150 275
Size: 1451
Fragments arranged by size:
1451 1059 936 635 541 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2295 2316 3759 4157 4431 4578 4989 2295
Size: 21 1443 398 274 147 411 2632
Fragments arranged by size:
2632 1443 411 398 274 147 21
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5101
Fragments arranged by size:
5101 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5326
BseYI C'CCAG_C
Cuts at: 2076 2213 2335 2439 2460 2488 2543 4529 5025
Size: 137 122 104 21 28 55 1986 496
Cuts at: 5025 2076
Size: 2377
Fragments arranged by size:
2377 1986 496 137 122 104 55 28
21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2249 2523 1163
Size: 573 513 274 3966
Fragments arranged by size:
3966 573 513 274
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2776 3537 4565 1540
Size: 275 186 775 761 1028 2301
Fragments arranged by size:
2301 1028 775 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2184 2482 3387 3472 4543 2184
Size: 298 905 85 1071 2967
Fragments arranged by size:
2967 1071 905 298 85
BsmI GAATG_Cn'
Cuts at: 2378 2837 2930 2378
Size: 459 93 4774
Fragments arranged by size:
4774 459 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 2169 2184 2192 2214 2277 2482
Size: 399 222 157 15 8 22 63 205
Cuts at: 2482 2645 3387 3472 4543 1391
Size: 163 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 222 205 163 157
85 63 22 15 8
BspEI T'CCGG_A
Cuts at: 2295 4989 2295
Size: 2694 2632
BspHI T'CATG_A
Cuts at: 2310 3215 4945 2310
Size: 905 1730 2691
Fragments arranged by size:
2691 1730 905
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2150 2581 2681 844
Size: 1306 431 100 3489
Fragments arranged by size:
3489 1306 431 100
BsrBI CCG'CTC
Cuts at: 1394 1823 3213 1394
Size: 429 1390 3507
Fragments arranged by size:
3507 1390 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3821 3995 65
Size: 1183 2573 174 1396
Fragments arranged by size:
2573 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3970 4157 1700
Size: 110 2160 187 2869
Fragments arranged by size:
2869 2160 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5326
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5326
BssSI C'ACGA_G
Cuts at: 3386 4398 3386
Size: 1012 4314
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5326
BstBI TT'CG_AA
Cuts at: 4130 4130
Size: 5326
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5326
BstXI CCAn_nnnn'nTGG
Cuts at: 2206 2206
Size: 5326
BstYI r'GATC_y
Cuts at: 1972 2304 2656 3023 3408 3425 4877 4963 4975
Size: 332 352 367 385 17 1452 86 12
Cuts at: 4975 5321 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 385 367 352 346 332 86
17 12
BstZ17I GTA'TAC
Cuts at: 2130 2130
Size: 5326
BtgI C'CryG_G
Cuts at: 513 1065 2193 513
Size: 552 1128 3646
Fragments arranged by size:
3646 1128 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4587
Fragments arranged by size:
4587 683 56
BtsI GCAGTG_nn'
Cuts at: 2842 3636 3656 2842
Size: 794 20 4512
Fragments arranged by size:
4512 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5326
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5291
DraI TTT'AAA
Cuts at: 2630 2630
Size: 5326
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4333 809
Size: 507 3017 1802
Fragments arranged by size:
3017 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2092 2196 2773
Size: 54 1425 50 275 18 262 104 577
Cuts at: 2773 3662 5291 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 577 275 262 104 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2773 1537
Size: 275 961 4090
Fragments arranged by size:
4090 961 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3256 1353
Size: 1903 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2009 3913 4287 4433 387
Size: 1064 138 420 1904 374 146 1280
Fragments arranged by size:
1904 1280 1064 420 374 146 138
EcoICRI GAG'CTC
Cuts at: 2182 2182
Size: 5326
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2285 2496 3389 3986 4773 5041 1239
Size: 393 653 211 893 597 787 268 1524
Fragments arranged by size:
1524 893 787 653 597 393 268 211
EcoO109I rG'GnC_Cy
Cuts at: 2065 2522 2065
Size: 457 4869
EcoRI G'AATT_C
Cuts at: 2635 3013 2635
Size: 378 4948
FspI TGC'GCA
Cuts at: 3832 3832
Size: 5326
HaeII r_GCGC'y
Cuts at: 1678 2236 2559 4473 1678
Size: 558 323 1914 2531
Fragments arranged by size:
2531 1914 558 323
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3967 3999 4041 4073 1373
Size: 32 135 32 2395 32 42 32 2626
Fragments arranged by size:
2626 2395 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2670 2916 669
Size: 988 281 732 246 3079
Fragments arranged by size:
3079 988 732 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5326
HpaI GTT'AAC
Cuts at: 2916 2916
Size: 5326
KpnI G_GTAC'C
Cuts at: 2323 2651 2323
Size: 328 4998
MfeI C'AATT_G
Cuts at: 2925 2925
Size: 5326
MluI A'CGCG_T
Cuts at: 5202 5202
Size: 5326
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4440 4624 1305
Size: 3135 184 2007
Fragments arranged by size:
3135 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2094 2198 10
Size: 54 1425 343 262 104 3138
Fragments arranged by size:
3138 1425 343 262 104 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3284 3643 3802 518
Size: 648 2118 359 159 2042
Fragments arranged by size:
2118 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2050 2206 2217 2335 2492 2579 2585 2742
Size: 444 156 11 118 157 87 6 157
Cuts at: 2742 3419 4567 4812 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 444 245 157 157 156
118 87 11 6
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4774
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5326
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5326
NotI GC'GGCC_GC
Cuts at: 2773 2773
Size: 5326
NspI r_CATG'y
Cuts at: 1132 1229 2684 4229 1132
Size: 97 1455 1545 2229
Fragments arranged by size:
2229 1545 1455 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5326
PciI A'CATG_T
Cuts at: 4225 4225
Size: 5326
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5326
PmeI GTTT'AAAC
Cuts at: 2630 2630
Size: 5326
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3395 3427 4908 4940 3395
Size: 32 1481 32 3781
Fragments arranged by size:
3781 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5326
PsiI TTA'TAA
Cuts at: 2896 2896
Size: 5326
PstI C_TGCA'G
Cuts at: 830 2049 2678 830
Size: 1219 629 3478
Fragments arranged by size:
3478 1219 629
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5326
PvuII CAG'CTG
Cuts at: 2050 2206 2217 2335 2492 2050
Size: 156 11 118 157 4884
Fragments arranged by size:
4884 157 156 118 11
SacI G_AGCT'C
Cuts at: 2184 2184
Size: 5326
SalI G'TCGA_C
Cuts at: 1936 2668 1936
Size: 732 4594
SbfI CC_TGCA'GG
Cuts at: 2678 2678
Size: 5326
ScaI AGT'ACT
Cuts at: 3574 5286 3574
Size: 1712 3614
SfcI C'TryA_G
Cuts at: 826 1045 2045 2674 3809 4490 4681 826
Size: 219 1000 629 1135 681 191 1471
Fragments arranged by size:
1471 1135 1000 681 629 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5326
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5326
SmaI CCC'GGG
Cuts at: 1872 2153 2653 1872
Size: 281 500 4545
Fragments arranged by size:
4545 500 281
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3430 4331 4593 820
Size: 197 926 1487 901 262 1553
Fragments arranged by size:
1553 1487 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3117 493
Size: 2624 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5326
SphI G_CATG'C
Cuts at: 1132 2684 1132
Size: 1552 3774
SspI AAT'ATT
Cuts at: 5 52 3250 5
Size: 47 3198 2081
Fragments arranged by size:
3198 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5326
StyI C'CwwG_G
Cuts at: 513 1065 2613 2765 513
Size: 552 1548 152 3074
Fragments arranged by size:
3074 1548 552 152
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2516 2557 3353 3370 3523 3708 4153
Size: 1255 936 41 796 17 153 185 445
Cuts at: 4153 325
Size: 1498
Fragments arranged by size:
1498 1255 936 796 445 185 153 41
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3572 5284 96
Size: 274 80 33 51 1443 1595 1712 138
Fragments arranged by size:
1712 1595 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5286 5318 5286
Size: 32 5294
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5326
XbaI T'CTAG_A
Cuts at: 2662 2662
Size: 5326
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5326
XmaI C'CCGG_G
Cuts at: 1870 2151 2651 1870
Size: 281 500 4545
Fragments arranged by size:
4545 500 281
XmnI GAAnn'nnTTC
Cuts at: 3455 3455
Size: 5326
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3134 276
Size: 53 83 186 936 1600 2468
Fragments arranged by size:
2468 1600 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI Bsu36I DraIII EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI