Restriction map for
pFN21AE1351
(Circular) (Six-base) MAPSORT of: pFN21AE1351.seq Check: 6406 from: 1 to: 6616
pFN21AE1351 6616 bp
With 182 enzymes: SgfI *
August 16, 2010 14:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4426 278
Size: 53 83 186 936 2890 2468
Fragments arranged by size:
2890 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3959 1937
Size: 2022 4594
Acc65I G'GTAC_C
Cuts at: 2467 3937 2467
Size: 1470 5146
AclI AA'CG_TT
Cuts at: 1563 4743 5116 1563
Size: 3180 373 3063
Fragments arranged by size:
3180 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2924 3550 4679 6063 6331 1632
Size: 1292 626 1129 1384 268 1917
Fragments arranged by size:
1917 1384 1292 1129 626 268
AflII C'TTAA_G
Cuts at: 820 1017 2863 820
Size: 197 1846 4573
Fragments arranged by size:
4573 1846 197
AflIII A'CryG_T
Cuts at: 1203 5515 6492 1203
Size: 4312 977 1327
Fragments arranged by size:
4312 1327 977
AgeI A'CCGG_T
Cuts at: 5447 5447
Size: 6616
AhdI GACnn_n'nnGTC
Cuts at: 5345 5345
Size: 6616
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6616
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6616
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2721 2753 2777 2809 2721
Size: 32 24 32 6528
Fragments arranged by size:
6528 32 32 24
AlwNI CAG_nnn'CTG
Cuts at: 2335 2947 3085 4359 5931 2335
Size: 612 138 1274 1572 3020
Fragments arranged by size:
3020 1572 1274 612 138
ApaLI G'TGCA_C
Cuts at: 4673 5829 4673
Size: 1156 5460
ApoI r'AATT_y
Cuts at: 1493 2338 3620 3925 4151 4303 6412 1493
Size: 845 1282 305 226 152 2109 1697
Fragments arranged by size:
2109 1697 1282 845 305 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5170 160
Size: 872 4138 1606
Fragments arranged by size:
4138 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6616
AvaI C'yCGr_G
Cuts at: 1870 1943 3398 3941 1870
Size: 73 1455 543 4545
Fragments arranged by size:
4545 1455 543 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3060 3093 3060
Size: 33 6583
BamHI G'GATC_C
Cuts at: 3946 4313 3946
Size: 367 6249
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2467 2479 2807 3937 5392
Size: 325 217 627 680 12 328 1130 1455
Cuts at: 5392 618
Size: 1842
Fragments arranged by size:
1842 1455 1130 680 627 328 325 217
12
BanII G_rGCy'C
Cuts at: 1391 2679 3320 3364 3935 1391
Size: 1288 641 44 571 4072
Fragments arranged by size:
4072 1288 641 571 44
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2131 2351 2925 928
Size: 971 232 220 574 4619
Fragments arranged by size:
4619 971 574 232 220
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4807 4841 1903
Size: 34 2870 34 3678
Fragments arranged by size:
3678 2870 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3061 3713 4508 5718 3061
Size: 652 795 1210 3959
Fragments arranged by size:
3959 1210 795 652
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6616
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 3146 5227
Size: 107 122 71 631 605 337 1137 2081
Cuts at: 5227 136
Size: 1525
Fragments arranged by size:
2081 1525 1137 631 605 337 122 107
71
BglII A'GATC_T
Cuts at: 6611 6611
Size: 6616
BlpI GC'TnA_GC
Cuts at: 4033 4033
Size: 6616
Bme1580I G_kGCm'C
Cuts at: 1790 4677 5833 1790
Size: 2887 1156 2573
Fragments arranged by size:
2887 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6616
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3487 4347 5305
Size: 416 53 83 399 24 2070 860 958
Cuts at: 5305 442
Size: 1753
Fragments arranged by size:
2070 1753 958 860 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6616
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2854 3736 2854
Size: 882 5734
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3264 5276 1239
Size: 2025 2012 2579
Fragments arranged by size:
2579 2025 2012
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2762 4741 5606 5904 2762
Size: 1979 865 298 3474
Fragments arranged by size:
3474 1979 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5279 882
Size: 617 3780 2219
Fragments arranged by size:
3780 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3782 4407 493
Size: 711 2578 625 2702
Fragments arranged by size:
2702 2578 711 625
BsaBI GATnn'nnATC
Cuts at: 6610 6610
Size: 6616
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4423 4805 5440 275
Size: 53 83 186 936 2890 382 635 1451
Fragments arranged by size:
2890 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2580 5049 5447 5721 5868 6279 2580
Size: 2469 398 274 147 411 2917
Fragments arranged by size:
2917 2469 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2543 2573 2777 2807 3706
Size: 30 165 30 1140 30 204 30 899
Cuts at: 3706 3736 1178
Size: 30 4058
Fragments arranged by size:
4058 1140 899 204 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2549 2564 2765 3074 1186
Size: 1363 15 201 309 4728
Fragments arranged by size:
4728 1363 309 201 15
BseYI C'CCAG_C
Cuts at: 3094 3363 3549 5819 6315 3094
Size: 269 186 2270 496 3395
Fragments arranged by size:
3395 2270 496 269 186
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2810 3506 3528 1163
Size: 573 1074 696 22 4251
Fragments arranged by size:
4251 1074 696 573 22
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2022 3154 4066 4827 5855 1540
Size: 275 186 21 1132 912 761 1028 2301
Fragments arranged by size:
2301 1132 1028 912 761 275 186 21
BsiHKAI G_wGCw'C
Cuts at: 4677 4762 5833 4677
Size: 85 1071 5460
Fragments arranged by size:
5460 1071 85
BsmI GAATG_Cn'
Cuts at: 2518 4127 4220 2518
Size: 1609 93 4914
Fragments arranged by size:
4914 1609 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2679 3320 3364 3935 4677 4762 5833
Size: 399 889 641 44 571 742 85 1071
Cuts at: 5833 1391
Size: 2174
Fragments arranged by size:
2174 1071 889 742 641 571 399 85
44
BspEI T'CCGG_A
Cuts at: 2580 6279 2580
Size: 3699 2917
BspHI T'CATG_A
Cuts at: 4505 6235 4505
Size: 1730 4886
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2185 3971 844
Size: 1341 1786 3489
Fragments arranged by size:
3489 1786 1341
BsrBI CCG'CTC
Cuts at: 1394 1823 3897 4503 1394
Size: 429 2074 606 3507
Fragments arranged by size:
3507 2074 606 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5111 5285 65
Size: 1183 3863 174 1396
Fragments arranged by size:
3863 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3153 3659 5260 5447 1700
Size: 110 1343 506 1601 187 2869
Fragments arranged by size:
2869 1601 1343 506 187 110
BsrGI T'GTAC_A
Cuts at: 96 3444 96
Size: 3348 3268
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6616
BssSI C'ACGA_G
Cuts at: 4676 5688 4676
Size: 1012 5604
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6616
BstBI TT'CG_AA
Cuts at: 5420 5420
Size: 6616
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6616
BstYI r'GATC_y
Cuts at: 1972 3583 3946 4313 4698 4715 6167 6253 6265
Size: 1611 363 367 385 17 1452 86 12
Cuts at: 6265 6611 1972
Size: 346 1977
Fragments arranged by size:
1977 1611 1452 385 367 363 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3486 3486
Size: 6616
BtgI C'CryG_G
Cuts at: 513 1065 2004 2267 3195 513
Size: 552 939 263 928 3934
Fragments arranged by size:
3934 939 928 552 263
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2027 3829 487
Size: 683 56 801 1802 3274
Fragments arranged by size:
3274 1802 801 683 56
BtsI GCAGTG_nn'
Cuts at: 2619 4132 4926 4946 2619
Size: 1513 794 20 4289
Fragments arranged by size:
4289 1513 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6616
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3177 3212 529
Size: 35 2613 35 3933
Fragments arranged by size:
3933 2613 35 35
DraI TTT'AAA
Cuts at: 3920 3920
Size: 6616
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3299 5623 809
Size: 507 1983 2324 1802
Fragments arranged by size:
2324 1983 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2103 2319 2406
Size: 54 1425 50 275 18 273 216 87
Cuts at: 2406 2437 4063 4952 6581 8
Size: 31 1626 889 1629 43
Fragments arranged by size:
1629 1626 1425 889 275 273 216 87
54 50 43 31 18
EagI C'GGCC_G
Cuts at: 1537 1812 4063 1537
Size: 275 2251 4090
Fragments arranged by size:
4090 2251 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2452 3051 3436 3758 4546 1353
Size: 1099 599 385 322 788 3423
Fragments arranged by size:
3423 1099 788 599 385 322
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3172 5203 5577 5723 387
Size: 1064 138 1583 2031 374 146 1280
Fragments arranged by size:
2031 1583 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2924 3264 3550 4679 5276 6063 6331
Size: 393 1292 340 286 1129 597 787 268
Cuts at: 6331 1239
Size: 1524
Fragments arranged by size:
1524 1292 1129 787 597 393 340 286
268
EcoNI CCTnn'n_nnAGG
Cuts at: 2384 2384
Size: 6616
EcoO109I rG'GnC_Cy
Cuts at: 2059 2488 2992 3110 2059
Size: 429 504 118 5565
Fragments arranged by size:
5565 504 429 118
EcoRI G'AATT_C
Cuts at: 3925 4303 3925
Size: 378 6238
FspI TGC'GCA
Cuts at: 5122 5122
Size: 6616
HaeII r_GCGC'y
Cuts at: 1678 5763 1678
Size: 4085 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2627 2659 2941 2973 3691
Size: 32 135 32 1055 32 282 32 718
Cuts at: 3691 3706 3723 3738 5257 5289 5331 5363 1373
Size: 15 17 15 1519 32 42 32 2626
Fragments arranged by size:
2626 1519 1055 718 282 135 42 32
32 32 32 32 32 17 15 15
HincII GTy'rAC
Cuts at: 669 1657 1938 3960 4206 669
Size: 988 281 2022 246 3079
Fragments arranged by size:
3079 2022 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2258 748
Size: 1510 5106
HpaI GTT'AAC
Cuts at: 4206 4206
Size: 6616
KpnI G_GTAC'C
Cuts at: 2471 3941 2471
Size: 1470 5146
MfeI C'AATT_G
Cuts at: 4215 4215
Size: 6616
MluI A'CGCG_T
Cuts at: 6492 6492
Size: 6616
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2763 5730 5914 1305
Size: 1458 2967 184 2007
Fragments arranged by size:
2967 2007 1458 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2105 2408 2439 10
Size: 54 1425 343 273 303 31 4187
Fragments arranged by size:
4187 1425 343 303 273 54 31
MslI CAynn'nnrTG
Cuts at: 518 1166 3800 4574 4933 5092 518
Size: 648 2634 774 359 159 2042
Fragments arranged by size:
2634 2042 774 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2052 4032 4709 5857 6102 1606
Size: 446 1980 677 1148 245 2120
Fragments arranged by size:
2120 1980 1148 677 446 245
NaeI GCC'GGC
Cuts at: 3661 3661
Size: 6616
NcoI C'CATG_G
Cuts at: 513 1065 2004 2267 513
Size: 552 939 263 4862
Fragments arranged by size:
4862 939 552 263
NdeI CA'TA_TG
Cuts at: 387 2974 387
Size: 2587 4029
NgoMIV G'CCGG_C
Cuts at: 3659 3659
Size: 6616
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6616
NotI GC'GGCC_GC
Cuts at: 4063 4063
Size: 6616
NspI r_CATG'y
Cuts at: 1132 1229 2134 3974 5519 1132
Size: 97 905 1840 1545 2229
Fragments arranged by size:
2229 1840 1545 905 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6616
PciI A'CATG_T
Cuts at: 5515 5515
Size: 6616
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2457 2854 1108
Size: 1349 397 4870
Fragments arranged by size:
4870 1349 397
PmeI GTTT'AAAC
Cuts at: 3920 3920
Size: 6616
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2777 2809 2816 2848 4685 4717 6198 6230 2777
Size: 32 7 32 1837 32 1481 32 3163
Fragments arranged by size:
3163 1837 1481 32 32 32 32 7
PpuMI rG'GwC_Cy
Cuts at: 2059 2488 2992 3110 2059
Size: 429 504 118 5565
Fragments arranged by size:
5565 504 429 118
PshAI GACnn'nnGTC
Cuts at: 1613 3248 1613
Size: 1635 4981
PsiI TTA'TAA
Cuts at: 4186 4186
Size: 6616
PstI C_TGCA'G
Cuts at: 830 2931 3968 830
Size: 2101 1037 3478
Fragments arranged by size:
3478 2101 1037
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6616
SalI G'TCGA_C
Cuts at: 1936 3958 1936
Size: 2022 4594
SanDI GG'GwC_CC
Cuts at: 3110 3110
Size: 6616
SbfI CC_TGCA'GG
Cuts at: 3968 3968
Size: 6616
ScaI AGT'ACT
Cuts at: 3645 4864 6576 3645
Size: 1219 1712 3685
Fragments arranged by size:
3685 1712 1219
SfcI C'TryA_G
Cuts at: 826 1045 2596 2927 3463 3964 5099 5780 5971
Size: 219 1551 331 536 501 1135 681 191
Cuts at: 5971 826
Size: 1471
Fragments arranged by size:
1551 1471 1135 681 536 501 331 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6616
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6616
SmaI CCC'GGG
Cuts at: 1872 3400 3943 1872
Size: 1528 543 4545
Fragments arranged by size:
4545 1528 543
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2777 2863 4720 5621 5883 820
Size: 197 926 834 86 1857 901 262 1553
Fragments arranged by size:
1857 1553 926 901 834 262 197 86
SnaBI TAC'GTA
Cuts at: 493 4407 493
Size: 3914 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6616
SphI G_CATG'C
Cuts at: 1132 2134 3974 1132
Size: 1002 1840 3774
Fragments arranged by size:
3774 1840 1002
SspI AAT'ATT
Cuts at: 5 52 4540 5
Size: 47 4488 2081
Fragments arranged by size:
4488 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6616
StyI C'CwwG_G
Cuts at: 513 1065 2004 2267 2472 2907 3615 4055 513
Size: 552 939 263 205 435 708 440 3074
Fragments arranged by size:
3074 939 708 552 440 435 263 205
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3060 3262 4643 4660 4813 4998 5443
Size: 1255 1480 202 1381 17 153 185 445
Cuts at: 5443 325
Size: 1498
Fragments arranged by size:
1498 1480 1381 1255 445 202 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2538 3405 3444
Size: 274 80 33 51 1443 561 867 39
Cuts at: 3444 3643 4862 6574 96
Size: 199 1219 1712 138
Fragments arranged by size:
1712 1443 1219 867 561 274 199 138
80 51 39 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2543 2575 2607 3102 3134 3225 3257 6576 6608
Size: 32 32 495 32 91 32 3319 32
Cuts at: 6608 2543
Size: 2551
Fragments arranged by size:
3319 2551 495 91 32 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6616
XbaI T'CTAG_A
Cuts at: 3952 3952
Size: 6616
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2432 2011
Size: 421 6195
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6616
XmaI C'CCGG_G
Cuts at: 1870 3398 3941 1870
Size: 1528 543 4545
Fragments arranged by size:
4545 1528 543
XmnI GAAnn'nnTTC
Cuts at: 2260 2831 4745 2260
Size: 571 1914 4131
Fragments arranged by size:
4131 1914 571
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4424 276
Size: 53 83 186 936 2890 2468
Fragments arranged by size:
2890 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI SalI SanDI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BbeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV
FalI FseI FspAI KasI NarI NruI NsiI PacI
PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SapI SexAI SfiI SfoI SrfI SwaI