Restriction map for
pFN21AE1350
(Circular) (Six-base) MAPSORT of: pFN21AE1350.seq Check: 1518 from: 1 to: 8266
pFN21AE1350 8266 bp
With 182 enzymes: SgfI *
August 16, 2010 14:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4635 6076 278
Size: 53 83 186 936 3099 1441 2468
Fragments arranged by size:
3099 2468 1441 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2516 5609 1937
Size: 579 3093 4594
Fragments arranged by size:
4594 3093 579
Acc65I G'GTAC_C
Cuts at: 5587 5587
Size: 8266
AclI AA'CG_TT
Cuts at: 1563 6393 6766 1563
Size: 4830 373 3063
Fragments arranged by size:
4830 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2108 5243 5531 5532 6329 7713 7981 1632
Size: 476 3135 288 1 797 1384 268 1917
Fragments arranged by size:
3135 1917 1384 797 476 288 268 1
AfeI AGC'GCT
Cuts at: 4688 4688
Size: 8266
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8069
AflIII A'CryG_T
Cuts at: 1203 3810 7165 8142 1203
Size: 2607 3355 977 1327
Fragments arranged by size:
3355 2607 1327 977
AgeI A'CCGG_T
Cuts at: 7097 7097
Size: 8266
AhdI GACnn_n'nnGTC
Cuts at: 6995 6995
Size: 8266
AleI CACnn'nnGTG
Cuts at: 1166 3158 1166
Size: 1992 6274
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2744 4607 5108 1184
Size: 1560 1863 501 4342
Fragments arranged by size:
4342 1863 1560 501
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5219 5251 5219
Size: 32 8234
AlwNI CAG_nnn'CTG
Cuts at: 2912 2966 4156 4549 5005 6009 7581 2912
Size: 54 1190 393 456 1004 1572 3597
Fragments arranged by size:
3597 1572 1190 1004 456 393 54
ApaI G_GGCC'C
Cuts at: 2385 2937 2385
Size: 552 7714
ApaLI G'TGCA_C
Cuts at: 6323 7479 6323
Size: 1156 7110
ApoI r'AATT_y
Cuts at: 1493 5324 5575 5801 5953 8062 1493
Size: 3831 251 226 152 2109 1697
Fragments arranged by size:
3831 2109 1697 251 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6820 160
Size: 872 5788 1606
Fragments arranged by size:
5788 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8266
AvaI C'yCGr_G
Cuts at: 1870 1943 2355 2792 3231 3401 3705 4505 4828
Size: 73 412 437 439 170 304 800 323
Cuts at: 4828 5591 1870
Size: 763 4545
Fragments arranged by size:
4545 800 763 439 437 412 323 304
170 73
BamHI G'GATC_C
Cuts at: 3354 4329 5596 5963 3354
Size: 975 1267 367 5657
Fragments arranged by size:
5657 1267 975 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2803 3481 4253 4298 4406
Size: 325 217 627 1016 678 772 45 108
Cuts at: 4406 5587 7042 618
Size: 1181 1455 1842
Fragments arranged by size:
1842 1455 1181 1016 772 678 627 325
217 108 45
BanII G_rGCy'C
Cuts at: 1391 2062 2130 2385 2874 2937 3706 4512 5315
Size: 671 68 255 489 63 769 806 803
Cuts at: 5315 5337 5585 1391
Size: 22 248 4072
Fragments arranged by size:
4072 806 803 769 671 489 255 248
68 63 22
BbeI G_GCGC'C
Cuts at: 4302 4410 4302
Size: 108 8158
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2633 2707 5519 928
Size: 971 734 74 2812 3675
Fragments arranged by size:
3675 2812 971 734 74
BbvCI CC'TCA_GC
Cuts at: 2163 3617 3731 4169 2163
Size: 1454 114 438 6260
Fragments arranged by size:
6260 1454 438 114
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 6457 6491 1903
Size: 34 50 34 4436 34 3678
Fragments arranged by size:
4436 3678 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6158 7368 6158
Size: 1210 7056
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8266
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4802 6877 136
Size: 107 122 71 631 605 3130 2075 1525
Fragments arranged by size:
3130 2075 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4422 4708 5022 8261 4422
Size: 286 314 3239 4427
Fragments arranged by size:
4427 3239 314 286
BlpI GC'TnA_GC
Cuts at: 3218 3740 5683 3218
Size: 522 1943 5801
Fragments arranged by size:
5801 1943 522
Bme1580I G_kGCm'C
Cuts at: 1790 2385 2832 2937 3800 4125 4256 4950 5151
Size: 595 447 105 863 325 131 694 201
Cuts at: 5151 6327 7483 1790
Size: 1176 1156 2573
Fragments arranged by size:
2573 1176 1156 863 694 595 447 325
201 131 105
BmgBI CAC'GTC
Cuts at: 1318 2494 1318
Size: 1176 7090
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2029 2113 5997
Size: 416 53 83 399 24 612 84 3884
Cuts at: 5997 6955 442
Size: 958 1753
Fragments arranged by size:
3884 1753 958 612 416 399 84 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8266
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2419 3941 2419
Size: 1522 6744
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2630 3233 3277 3392 3448 3572 4199 4291
Size: 1391 603 44 115 56 124 627 92
Cuts at: 4291 4439 5287 6926 1239
Size: 148 848 1639 2579
Fragments arranged by size:
2579 1639 1391 848 627 603 148 124
115 92 56 44
Bpu10I CC'TnA_GC
Cuts at: 2163 2241 3617 3731 4169 4376 5349 2163
Size: 78 1376 114 438 207 973 5080
Fragments arranged by size:
5080 1376 973 438 207 114 78
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4784 5351 6391 7256 7554 4784
Size: 567 1040 865 298 5496
Fragments arranged by size:
5496 1040 865 567 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4003 5064 6929 882
Size: 617 2504 1061 1865 2219
Fragments arranged by size:
2504 2219 1865 1061 617
BsaAI yAC'GTr
Cuts at: 493 1204 3160 4072 4945 6057 493
Size: 711 1956 912 873 1112 2702
Fragments arranged by size:
2702 1956 1112 912 873 711
BsaBI GATnn'nnATC
Cuts at: 3341 8260 3341
Size: 4919 3347
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4029 4299 4407 4632
Size: 53 83 186 936 2496 270 108 225
Cuts at: 4632 4661 5471 6073 6455 7090 275
Size: 29 810 602 382 635 1451
Fragments arranged by size:
2496 1451 936 810 635 602 382 270
225 186 108 83 53 29
BsaWI w'CCGG_w
Cuts at: 2331 6699 7097 7371 7518 7929 2331
Size: 4368 398 274 147 411 2668
Fragments arranged by size:
4368 2668 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3107 3137 5219 5249 5285
Size: 30 165 30 1704 30 2082 30 36
Cuts at: 5285 5315 1178
Size: 30 4129
Fragments arranged by size:
4129 2082 1704 165 36 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2196 2537 2540 2627 3008 3011 3026 3869
Size: 1010 341 3 87 381 3 15 843
Cuts at: 3869 4328 1186
Size: 459 5124
Fragments arranged by size:
5124 1010 843 459 381 341 87 15
3 3
BseYI C'CCAG_C
Cuts at: 2061 2440 2757 2930 3468 3558 3575 3774 4149
Size: 379 317 173 538 90 17 199 375
Cuts at: 4149 4237 4818 4998 5016 5457 7469 7965 2061
Size: 88 581 180 18 441 2012 496 2362
Fragments arranged by size:
2362 2012 581 538 496 441 379 375
317 199 180 173 90 88 18 17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2084 2555 3404 5030 1163
Size: 573 348 471 849 1626 4399
Fragments arranged by size:
4399 1626 849 573 471 348
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2520 3288 5716 6477 7505 1540
Size: 275 186 519 768 2428 761 1028 2301
Fragments arranged by size:
2428 2301 1028 768 761 519 275 186
BsiHKAI G_wGCw'C
Cuts at: 3177 3434 3927 4080 4878 5337 6327 6412 7483
Size: 257 493 153 798 459 990 85 1071
Cuts at: 7483 3177
Size: 3960
Fragments arranged by size:
3960 1071 990 798 493 459 257 153
85
BsmI GAATG_Cn'
Cuts at: 4355 5777 5870 4355
Size: 1422 93 6751
Fragments arranged by size:
6751 1422 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2062 2130 2385 2832 2874 2937 3177
Size: 399 272 68 255 447 42 63 240
Cuts at: 3177 3434 3706 3800 3927 4080 4125 4256 4512
Size: 257 272 94 127 153 45 131 256
Cuts at: 4512 4878 4950 5151 5315 5337 5585 6327 6412
Size: 366 72 201 164 22 248 742 85
Cuts at: 6412 7483 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 447 399 366 272 272
257 256 255 248 240 201 164 153
131 127 94 85 72 68 63 45
42 22
BspEI T'CCGG_A
Cuts at: 2331 7929 2331
Size: 5598 2668
BspHI T'CATG_A
Cuts at: 3987 5445 6155 7885 3987
Size: 1458 710 1730 4368
Fragments arranged by size:
4368 1730 1458 710
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2712 4980 5621 844
Size: 1868 2268 641 3489
Fragments arranged by size:
3489 2268 1868 641
BsrBI CCG'CTC
Cuts at: 1394 1823 2797 4111 4318 4827 6153 1394
Size: 429 974 1314 207 509 1326 3507
Fragments arranged by size:
3507 1326 1314 974 509 429 207
BsrDI GCAATG_nn'
Cuts at: 65 1248 2181 5343 6761 6935 65
Size: 1183 933 3162 1418 174 1396
Fragments arranged by size:
3162 1418 1396 1183 933 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2362 2664 3097 3192 3564 4497 4657
Size: 110 552 302 433 95 372 933 160
Cuts at: 4657 6910 7097 1700
Size: 2253 187 2869
Fragments arranged by size:
2869 2253 933 552 433 372 302 187
160 110 95
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8266
BssHII G'CGCG_C
Cuts at: 1925 5081 1925
Size: 3156 5110
BssSI C'ACGA_G
Cuts at: 2945 3311 3884 4871 5159 6326 7338 2945
Size: 366 573 987 288 1167 1012 3873
Fragments arranged by size:
3873 1167 1012 987 573 366 288
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3502 4501 794
Size: 2708 999 4559
Fragments arranged by size:
4559 2708 999
BstBI TT'CG_AA
Cuts at: 7070 7070
Size: 8266
BstEII G'GTnAC_C
Cuts at: 1184 5269 1184
Size: 4085 4181
BstXI CCAn_nnnn'nTGG
Cuts at: 4417 4417
Size: 8266
BstYI r'GATC_y
Cuts at: 1972 2280 2529 3354 4329 4422 4701 4708 5022
Size: 308 249 825 975 93 279 7 314
Cuts at: 5022 5596 5963 6348 6365 7817 7903 7915 8261
Size: 574 367 385 17 1452 86 12 346
Cuts at: 8261 1972
Size: 1977
Fragments arranged by size:
1977 1452 975 825 574 385 367 346
314 308 279 249 93 86 17 12
7
Bsu36I CC'TnA_GG
Cuts at: 2843 3852 2843
Size: 1009 7257
BtgI C'CryG_G
Cuts at: 513 1065 2067 2076 2596 3110 3326 3358 3901
Size: 552 1002 9 520 514 216 32 543
Cuts at: 3901 4125 4386 5408 513
Size: 224 261 1022 3371
Fragments arranged by size:
3371 1022 1002 552 543 520 514 261
224 216 32 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2136 3940 487
Size: 683 56 910 1804 4813
Fragments arranged by size:
4813 1804 910 683 56
BtsI GCAGTG_nn'
Cuts at: 3037 5782 6576 6596 3037
Size: 2745 794 20 4707
Fragments arranged by size:
4707 2745 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8266
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8231
DraI TTT'AAA
Cuts at: 5570 5570
Size: 8266
DraIII CAC_nnn'GTG
Cuts at: 2950 3163 3316 2950
Size: 213 153 7900
Fragments arranged by size:
7900 213 153
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5111 5470 7273 809
Size: 507 3795 359 1803 1802
Fragments arranged by size:
3795 1803 1802 507 359
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2028 2619 2984
Size: 54 1425 50 275 18 198 591 365
Cuts at: 2984 3091 3285 4158 4213 4527 5713 6602 8231
Size: 107 194 873 55 314 1186 889 1629
Cuts at: 8231 8
Size: 43
Fragments arranged by size:
1629 1425 1186 889 873 591 365 314
275 198 194 107 55 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3285 5713 1537
Size: 275 1473 2428 4090
Fragments arranged by size:
4090 2428 1473 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2237 5015 6196 1353
Size: 884 2778 1181 3423
Fragments arranged by size:
3423 2778 1181 884
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2582 2651 3266 6853 7227 7373
Size: 1064 138 993 69 615 3587 374 146
Cuts at: 7373 387
Size: 1280
Fragments arranged by size:
3587 1280 1064 993 615 374 146 138
69
EcoICRI GAG'CTC
Cuts at: 5335 5335
Size: 8266
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2108 2630 3233 3277 3392 3448 3572
Size: 393 476 522 603 44 115 56 124
Cuts at: 3572 4199 4291 4439 5243 5287 5531 5532 6329
Size: 627 92 148 804 44 244 1 797
Cuts at: 6329 6926 7713 7981 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 804 797 787 627 603 597 522
476 393 268 244 148 124 115 92
56 44 44 1
EcoNI CCTnn'n_nnAGG
Cuts at: 2612 5138 2612
Size: 2526 5740
EcoO109I rG'GnC_Cy
Cuts at: 2382 2934 3540 3847 3877 4441 5542 2382
Size: 552 606 307 30 564 1101 5106
Fragments arranged by size:
5106 1101 606 564 552 307 30
EcoRI G'AATT_C
Cuts at: 5575 5953 5575
Size: 378 7888
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2650 4756 2650
Size: 2106 6160
FspI TGC'GCA
Cuts at: 4859 6772 4859
Size: 1913 6353
HaeII r_GCGC'y
Cuts at: 1678 2688 3245 4167 4302 4410 4690 7413 1678
Size: 1010 557 922 135 108 280 2723 2531
Fragments arranged by size:
2723 2531 1010 922 557 280 135 108
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2429 2461 2483 2515 3623
Size: 32 135 32 857 32 22 32 1108
Cuts at: 3623 3655 4444 4476 4693 4725 5243 5275 6907
Size: 32 789 32 217 32 518 32 1632
Cuts at: 6907 6939 6981 7013 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1632 1108 857 789 518 217 135
42 32 32 32 32 32 32 32
32 32 32 22
HincII GTy'rAC
Cuts at: 669 1657 1938 2517 3757 5610 5856 669
Size: 988 281 579 1240 1853 246 3079
Fragments arranged by size:
3079 1853 1240 988 579 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8266
HpaI GTT'AAC
Cuts at: 5856 5856
Size: 8266
KasI G'GCGC_C
Cuts at: 4298 4406 4298
Size: 108 8158
KpnI G_GTAC'C
Cuts at: 5591 5591
Size: 8266
MfeI C'AATT_G
Cuts at: 5865 5865
Size: 8266
MluI A'CGCG_T
Cuts at: 8142 8142
Size: 8266
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4644 4781 7380 7564 1305
Size: 3339 137 2599 184 2007
Fragments arranged by size:
3339 2599 2007 184 137
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2030 2621 2986 4160 4529
Size: 54 1425 343 198 591 365 1174 369
Cuts at: 4529 10
Size: 3747
Fragments arranged by size:
3747 1425 1174 591 369 365 343 198
54
MslI CAynn'nnrTG
Cuts at: 518 1166 2503 3158 3692 4203 4385 6224 6583
Size: 648 1337 655 534 511 182 1839 359
Cuts at: 6583 6742 518
Size: 159 2042
Fragments arranged by size:
2042 1839 1337 655 648 534 511 359
182 159
MspA1I CmG'CkG
Cuts at: 1606 2162 3112 3778 3843 4156 4525 4930 5002
Size: 556 950 666 65 313 369 405 72
Cuts at: 5002 5682 6359 7507 7752 1606
Size: 680 677 1148 245 2120
Fragments arranged by size:
2120 1148 950 680 677 666 556 405
369 313 245 72 65
NaeI GCC'GGC
Cuts at: 2666 3099 2666
Size: 433 7833
NarI GG'CG_CC
Cuts at: 4299 4407 4299
Size: 108 8158
NcoI C'CATG_G
Cuts at: 513 1065 2067 2076 2596 4125 4386 513
Size: 552 1002 9 520 1529 261 4393
Fragments arranged by size:
4393 1529 1002 552 520 261 9
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8266
NgoMIV G'CCGG_C
Cuts at: 2664 3097 2664
Size: 433 7833
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8266
NotI GC'GGCC_GC
Cuts at: 5713 5713
Size: 8266
NspI r_CATG'y
Cuts at: 1132 1229 3496 5624 7169 1132
Size: 97 2267 2128 1545 2229
Fragments arranged by size:
2267 2229 2128 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2130 4951 1348
Size: 782 2821 4663
Fragments arranged by size:
4663 2821 782
PciI A'CATG_T
Cuts at: 7165 7165
Size: 8266
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2068 2764 3316 4131 4156 1108
Size: 960 696 552 815 25 5218
Fragments arranged by size:
5218 960 815 696 552 25
PfoI T'CCnGG_A
Cuts at: 2433 2883 3144 3345 4416 2433
Size: 450 261 201 1071 6283
Fragments arranged by size:
6283 1071 450 261 201
PmeI GTTT'AAAC
Cuts at: 5570 5570
Size: 8266
PmlI CAC'GTG
Cuts at: 3160 3160
Size: 8266
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4637 4669 5219 5251 6335 6367 7848 7880 4637
Size: 32 550 32 1084 32 1481 32 5023
Fragments arranged by size:
5023 1481 1084 550 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3847 3877 3847
Size: 30 8236
PshAI GACnn'nnGTC
Cuts at: 1613 3942 5421 1613
Size: 2329 1479 4458
Fragments arranged by size:
4458 2329 1479
PsiI TTA'TAA
Cuts at: 5836 5836
Size: 8266
PspOMI G'GGCC_C
Cuts at: 2381 2933 2381
Size: 552 7714
PspXI vC'TCGA_Gb
Cuts at: 2792 2792
Size: 8266
PstI C_TGCA'G
Cuts at: 830 4452 4614 4929 5618 830
Size: 3622 162 315 689 3478
Fragments arranged by size:
3622 3478 689 315 162
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8266
PvuII CAG'CTG
Cuts at: 2162 3778 4156 4525 4930 5002 2162
Size: 1616 378 369 405 72 5426
Fragments arranged by size:
5426 1616 405 378 369 72
SacI G_AGCT'C
Cuts at: 5337 5337
Size: 8266
SacII CC_GC'GG
Cuts at: 3113 3113
Size: 8266
SalI G'TCGA_C
Cuts at: 1936 2515 5608 1936
Size: 579 3093 4594
Fragments arranged by size:
4594 3093 579
SanDI GG'GwC_CC
Cuts at: 3847 3847
Size: 8266
SapI GCTCTTCn'nnn_
Cuts at: 5015 5015
Size: 8266
SbfI CC_TGCA'GG
Cuts at: 5618 5618
Size: 8266
ScaI AGT'ACT
Cuts at: 5165 6514 8226 5165
Size: 1349 1712 5205
Fragments arranged by size:
5205 1712 1349
SexAI A'CCwGG_T
Cuts at: 4191 4357 4191
Size: 166 8100
SfcI C'TryA_G
Cuts at: 826 1045 2904 4448 4610 4925 5614 6749 7430
Size: 219 1859 1544 162 315 689 1135 681
Cuts at: 7430 7621 826
Size: 191 1471
Fragments arranged by size:
1859 1544 1471 1135 689 681 315 219
191 162
SfoI GGC'GCC
Cuts at: 4300 4408 4300
Size: 108 8158
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8266
SgrAI Cr'CCGG_yG
Cuts at: 1700 4657 1700
Size: 2957 5309
SmaI CCC'GGG
Cuts at: 1872 3233 5593 1872
Size: 1361 2360 4545
Fragments arranged by size:
4545 2360 1361
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2792 4799 5330 6370 7271 7533
Size: 197 926 849 2007 531 1040 901 262
Cuts at: 7533 820
Size: 1553
Fragments arranged by size:
2007 1553 1040 926 901 849 531 262
197
SnaBI TAC'GTA
Cuts at: 493 6057 493
Size: 5564 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8266
SphI G_CATG'C
Cuts at: 1132 3496 5624 1132
Size: 2364 2128 3774
Fragments arranged by size:
3774 2364 2128
SspI AAT'ATT
Cuts at: 5 52 6190 5
Size: 47 6138 2081
Fragments arranged by size:
6138 2081 47
StuI AGG'CCT
Cuts at: 1517 2085 1517
Size: 568 7698
StyI C'CwwG_G
Cuts at: 513 1065 2067 2076 2596 3072 4125 4386 4909
Size: 552 1002 9 520 476 1053 261 523
Cuts at: 4909 5139 5533 5705 513
Size: 230 394 172 3074
Fragments arranged by size:
3074 1053 1002 552 523 520 476 394
261 230 172 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2534 3170 3788 6293 6310 6463 6648
Size: 1255 954 636 618 2505 17 153 185
Cuts at: 6648 7093 325
Size: 445 1498
Fragments arranged by size:
2505 1498 1255 954 636 618 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4743 4887 5163
Size: 274 80 33 51 1443 2766 144 276
Cuts at: 5163 6512 8224 96
Size: 1349 1712 138
Fragments arranged by size:
2766 1712 1443 1349 276 274 144 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3107 3139 3191 3223 5285 5317 8226 8258 3107
Size: 32 52 32 2062 32 2909 32 3115
Fragments arranged by size:
3115 2909 2062 52 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8266
XbaI T'CTAG_A
Cuts at: 5602 5602
Size: 8266
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2074 2074
Size: 8266
XhoI C'TCGA_G
Cuts at: 1943 2792 1943
Size: 849 7417
XmaI C'CCGG_G
Cuts at: 1870 3231 5591 1870
Size: 1361 2360 4545
Fragments arranged by size:
4545 2360 1361
XmnI GAAnn'nnTTC
Cuts at: 2714 6395 2714
Size: 3681 4585
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4633 6074 276
Size: 53 83 186 936 3099 1441 2468
Fragments arranged by size:
3099 2468 1441 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PstI PvuI PvuII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I
EcoRV FseI FspAI NruI NsiI PacI PsrI RsrII
SfiI SrfI SwaI