Restriction map for
pFN21AE1347
(Circular) (Six-base) MAPSORT of: pFN21AE1347.seq Check: 1591 from: 1 to: 5842
pFN21AE1347 5842 bp
With 182 enzymes: SgfI *
August 16, 2010 14:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3652 278
Size: 53 83 186 936 2116 2468
Fragments arranged by size:
2468 2116 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3185 1937
Size: 1248 4594
Acc65I G'GTAC_C
Cuts at: 3163 3163
Size: 5842
AclI AA'CG_TT
Cuts at: 1563 3969 4342 1563
Size: 2406 373 3063
Fragments arranged by size:
3063 2406 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2234 3905 5289 5557 1632
Size: 602 1671 1384 268 1917
Fragments arranged by size:
1917 1671 1384 602 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5645
AflIII A'CryG_T
Cuts at: 1203 4741 5718 1203
Size: 3538 977 1327
Fragments arranged by size:
3538 1327 977
AgeI A'CCGG_T
Cuts at: 4673 4673
Size: 5842
AhdI GACnn_n'nnGTC
Cuts at: 4571 4571
Size: 5842
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5842
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5842
AlwNI CAG_nnn'CTG
Cuts at: 2074 2406 3585 5157 2074
Size: 332 1179 1572 2759
Fragments arranged by size:
2759 1572 1179 332
ApaLI G'TGCA_C
Cuts at: 2445 2502 3899 5055 2445
Size: 57 1397 1156 3232
Fragments arranged by size:
3232 1397 1156 57
ApoI r'AATT_y
Cuts at: 1493 2243 2329 3151 3377 3529 5638 1493
Size: 750 86 822 226 152 2109 1697
Fragments arranged by size:
2109 1697 822 750 226 152 86
AseI AT'TA_AT
Cuts at: 160 1032 4396 160
Size: 872 3364 1606
Fragments arranged by size:
3364 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5842
AvaI C'yCGr_G
Cuts at: 1870 1943 2337 3167 1870
Size: 73 394 830 4545
Fragments arranged by size:
4545 830 394 73
AvrII C'CTAG_G
Cuts at: 2762 2762
Size: 5842
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2544 2577 2544
Size: 33 5809
BamHI G'GATC_C
Cuts at: 3172 3539 3172
Size: 367 5475
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3163 4618 618
Size: 325 217 627 1376 1455 1842
Fragments arranged by size:
1842 1455 1376 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2842 3161 1391
Size: 1451 319 4072
Fragments arranged by size:
4072 1451 319
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3033 928
Size: 971 1134 3737
Fragments arranged by size:
3737 1134 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4033 4067 1903
Size: 34 2096 34 3678
Fragments arranged by size:
3678 2096 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3109 3734 4944 3109
Size: 625 1210 4007
Fragments arranged by size:
4007 1210 625
BclI T'GATC_A
Cuts at: 1548 2397 2901 1548
Size: 849 504 4489
Fragments arranged by size:
4489 849 504
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4453 136
Size: 107 122 71 631 605 337 2444 1525
Fragments arranged by size:
2444 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 2890 3088 5837 2890
Size: 198 2749 2895
Fragments arranged by size:
2895 2749 198
BlpI GC'TnA_GC
Cuts at: 3259 3259
Size: 5842
Bme1580I G_kGCm'C
Cuts at: 1790 2449 2506 3903 5059 1790
Size: 659 57 1397 1156 2573
Fragments arranged by size:
2573 1397 1156 659 57
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5842
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2445 3573 4531
Size: 416 53 83 399 24 1028 1128 958
Cuts at: 4531 442
Size: 1753
Fragments arranged by size:
1753 1128 1028 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5842
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2096 2958 4502 1239
Size: 857 862 1544 2579
Fragments arranged by size:
2579 1544 862 857
Bpu10I CC'TnA_GC
Cuts at: 2727 2727
Size: 5842
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3967 4832 5130 3967
Size: 865 298 4679
Fragments arranged by size:
4679 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2318 4505 882
Size: 617 819 2187 2219
Fragments arranged by size:
2219 2187 819 617
BsaAI yAC'GTr
Cuts at: 493 1204 3633 493
Size: 711 2429 2702
Fragments arranged by size:
2702 2429 711
BsaBI GATnn'nnATC
Cuts at: 5836 5836
Size: 5842
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3649 4031 4666 275
Size: 53 83 186 936 2116 382 635 1451
Fragments arranged by size:
2116 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4275 4673 4947 5094 5505 4275
Size: 398 274 147 411 4612
Fragments arranged by size:
4612 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5617
Fragments arranged by size:
5617 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2051 2156 1186
Size: 865 105 4872
Fragments arranged by size:
4872 865 105
BseYI C'CCAG_C
Cuts at: 3102 5045 5541 3102
Size: 1943 496 3403
Fragments arranged by size:
3403 1943 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2087 1163
Size: 573 351 4918
Fragments arranged by size:
4918 573 351
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2022 2218 3292 4053 5081 1540
Size: 275 186 21 196 1074 761 1028 2301
Fragments arranged by size:
2301 1074 1028 761 275 196 186 21
BsiHKAI G_wGCw'C
Cuts at: 2449 2506 3903 3988 5059 2449
Size: 57 1397 85 1071 3232
Fragments arranged by size:
3232 1397 1071 85 57
BsmI GAATG_Cn'
Cuts at: 3353 3446 3353
Size: 93 5749
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2449 2506 2842 3161 3903 3988 5059
Size: 399 659 57 336 319 742 85 1071
Cuts at: 5059 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 659 399 336 319 85
57
BspEI T'CCGG_A
Cuts at: 5505 5505
Size: 5842
BspHI T'CATG_A
Cuts at: 3731 5461 3731
Size: 1730 4112
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2067 3197 844
Size: 1223 1130 3489
Fragments arranged by size:
3489 1223 1130
BsrBI CCG'CTC
Cuts at: 1394 1823 3729 1394
Size: 429 1906 3507
Fragments arranged by size:
3507 1906 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4337 4511 65
Size: 1183 3089 174 1396
Fragments arranged by size:
3089 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4486 4673 1700
Size: 110 2676 187 2869
Fragments arranged by size:
2869 2676 187 110
BsrGI T'GTAC_A
Cuts at: 96 2464 96
Size: 2368 3474
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5842
BssSI C'ACGA_G
Cuts at: 3902 4914 3902
Size: 1012 4830
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5842
BstBI TT'CG_AA
Cuts at: 4646 4646
Size: 5842
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5842
BstYI r'GATC_y
Cuts at: 1972 2624 2890 3088 3172 3539 3924 3941 5393
Size: 652 266 198 84 367 385 17 1452
Cuts at: 5393 5479 5491 5837 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 652 385 367 346 266 198
86 84 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4351
Fragments arranged by size:
4351 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2966 487
Size: 683 56 1740 3363
Fragments arranged by size:
3363 1740 683 56
BtsI GCAGTG_nn'
Cuts at: 2031 2303 3358 4152 4172 2031
Size: 272 1055 794 20 3701
Fragments arranged by size:
3701 1055 794 272 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5842
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5807
DraI TTT'AAA
Cuts at: 2202 2850 3146 2202
Size: 648 296 4898
Fragments arranged by size:
4898 648 296
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4849 809
Size: 507 3533 1802
Fragments arranged by size:
3533 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2019 2031 3289
Size: 54 1425 50 275 18 189 12 1258
Cuts at: 3289 4178 5807 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1258 889 275 189 54 50
43 18 12
EagI C'GGCC_G
Cuts at: 1537 1812 2019 3289 1537
Size: 275 207 1270 4090
Fragments arranged by size:
4090 1270 275 207
EarI CTCTTCn'nnn_
Cuts at: 1353 3009 3772 1353
Size: 1656 763 3423
Fragments arranged by size:
3423 1656 763
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4429 4803 4949 387
Size: 1064 138 2840 374 146 1280
Fragments arranged by size:
2840 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2096 2234 2958 3905 4502 5289 5557
Size: 393 464 138 724 947 597 787 268
Cuts at: 5557 1239
Size: 1524
Fragments arranged by size:
1524 947 787 724 597 464 393 268
138
EcoO109I rG'GnC_Cy
Cuts at: 2056 2089 2056
Size: 33 5809
EcoRI G'AATT_C
Cuts at: 3151 3529 3151
Size: 378 5464
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2465 2687 3047 2465
Size: 222 360 5260
Fragments arranged by size:
5260 360 222
FspI TGC'GCA
Cuts at: 4348 4348
Size: 5842
HaeII r_GCGC'y
Cuts at: 1678 2407 4989 1678
Size: 729 2582 2531
Fragments arranged by size:
2582 2531 729
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4483 4515 4557 4589 1373
Size: 32 135 32 2911 32 42 32 2626
Fragments arranged by size:
2911 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2411 3186 3432 669
Size: 988 281 473 775 246 3079
Fragments arranged by size:
3079 988 775 473 281 246
HindIII A'AGCT_T
Cuts at: 748 3068 748
Size: 2320 3522
HpaI GTT'AAC
Cuts at: 3432 3432
Size: 5842
KpnI G_GTAC'C
Cuts at: 3167 3167
Size: 5842
MfeI C'AATT_G
Cuts at: 3441 3441
Size: 5842
MluI A'CGCG_T
Cuts at: 5718 5718
Size: 5842
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4956 5140 1305
Size: 3651 184 2007
Fragments arranged by size:
3651 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4020
Fragments arranged by size:
4020 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3800 4159 4318 518
Size: 648 2634 359 159 2042
Fragments arranged by size:
2634 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2085 2123 3258 3935 5083 5328 1606
Size: 479 38 1135 677 1148 245 2120
Fragments arranged by size:
2120 1148 1135 677 479 245 38
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4351
Fragments arranged by size:
4351 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5842
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5842
NotI GC'GGCC_GC
Cuts at: 2019 3289 2019
Size: 1270 4572
NspI r_CATG'y
Cuts at: 1132 1229 3200 4745 1132
Size: 97 1971 1545 2229
Fragments arranged by size:
2229 1971 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5842
PciI A'CATG_T
Cuts at: 4741 4741
Size: 5842
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2435 1108
Size: 1327 4515
PmeI GTTT'AAAC
Cuts at: 3146 3146
Size: 5842
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3911 3943 5424 5456 3911
Size: 32 1481 32 4297
Fragments arranged by size:
4297 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2056 2089 2056
Size: 33 5809
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5842
PsiI TTA'TAA
Cuts at: 3412 3412
Size: 5842
PstI C_TGCA'G
Cuts at: 830 3194 830
Size: 2364 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5842
PvuII CAG'CTG
Cuts at: 2123 2123
Size: 5842
SalI G'TCGA_C
Cuts at: 1936 3184 1936
Size: 1248 4594
SbfI CC_TGCA'GG
Cuts at: 3194 3194
Size: 5842
ScaI AGT'ACT
Cuts at: 4090 5802 4090
Size: 1712 4130
SfcI C'TryA_G
Cuts at: 826 1045 3190 4325 5006 5197 826
Size: 219 2145 1135 681 191 1471
Fragments arranged by size:
2145 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5842
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5842
SmaI CCC'GGG
Cuts at: 1872 3169 1872
Size: 1297 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3946 4847 5109 820
Size: 197 926 2003 901 262 1553
Fragments arranged by size:
2003 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3633 493
Size: 3140 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5842
SphI G_CATG'C
Cuts at: 1132 3200 1132
Size: 2068 3774
SspI AAT'ATT
Cuts at: 5 52 3766 5
Size: 47 3714 2081
Fragments arranged by size:
3714 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5842
StyI C'CwwG_G
Cuts at: 513 1065 2004 2762 3281 513
Size: 552 939 758 519 3074
Fragments arranged by size:
3074 939 758 552 519
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2363 3869 3886 4039 4224 4669 325
Size: 1255 783 1506 17 153 185 445 1498
Fragments arranged by size:
1506 1498 1255 783 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2464 4088 5800
Size: 274 80 33 51 1443 487 1624 1712
Cuts at: 5800 96
Size: 138
Fragments arranged by size:
1712 1624 1443 487 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2588 2620 5802 5834 2588
Size: 32 3182 32 2596
Fragments arranged by size:
3182 2596 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5842
XbaI T'CTAG_A
Cuts at: 3178 3178
Size: 5842
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5842
XmaI C'CCGG_G
Cuts at: 1870 3167 1870
Size: 1297 4545
XmnI GAAnn'nnTTC
Cuts at: 2247 3971 2247
Size: 1724 4118
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3650 276
Size: 53 83 186 936 2116 2468
Fragments arranged by size:
2468 2116 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI