Restriction map for
pFN21AE1346
(Circular) (Six-base) MAPSORT of: pFN21AE1346.seq Check: 4565 from: 1 to: 5470
pFN21AE1346 5470 bp
With 182 enzymes: SgfI *
August 16, 2010 14:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3280 278
Size: 53 83 186 936 1744 2468
Fragments arranged by size:
2468 1744 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2813 1937
Size: 876 4594
Acc65I G'GTAC_C
Cuts at: 2791 2791
Size: 5470
AclI AA'CG_TT
Cuts at: 1563 3597 3970 1563
Size: 2034 373 3063
Fragments arranged by size:
3063 2034 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2102 3533 4917 5185 1632
Size: 470 1431 1384 268 1917
Fragments arranged by size:
1917 1431 1384 470 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5273
AflIII A'CryG_T
Cuts at: 1203 4369 5346 1203
Size: 3166 977 1327
Fragments arranged by size:
3166 1327 977
AgeI A'CCGG_T
Cuts at: 4301 4301
Size: 5470
AhdI GACnn_n'nnGTC
Cuts at: 4199 4199
Size: 5470
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5470
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2212 1184
Size: 1028 4442
AlwNI CAG_nnn'CTG
Cuts at: 3213 4785 3213
Size: 1572 3898
ApaLI G'TGCA_C
Cuts at: 2428 3527 4683 2428
Size: 1099 1156 3215
Fragments arranged by size:
3215 1156 1099
ApoI r'AATT_y
Cuts at: 1493 2779 3005 3157 5266 1493
Size: 1286 226 152 2109 1697
Fragments arranged by size:
2109 1697 1286 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4024 160
Size: 872 2992 1606
Fragments arranged by size:
2992 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5470
AvaI C'yCGr_G
Cuts at: 1870 1943 2417 2795 1870
Size: 73 474 378 4545
Fragments arranged by size:
4545 474 378 73
BamHI G'GATC_C
Cuts at: 2800 3167 2800
Size: 367 5103
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2228 2791 4246 618
Size: 325 217 627 441 563 1455 1842
Fragments arranged by size:
1842 1455 627 563 441 325 217
BanII G_rGCy'C
Cuts at: 1391 2401 2789 1391
Size: 1010 388 4072
Fragments arranged by size:
4072 1010 388
BbeI G_GCGC'C
Cuts at: 2232 2232
Size: 5470
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2178 2677 928
Size: 971 279 499 3721
Fragments arranged by size:
3721 971 499 279
BbvCI CC'TCA_GC
Cuts at: 2052 2052
Size: 5470
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3661 3695 1903
Size: 34 50 34 1640 34 3678
Fragments arranged by size:
3678 1640 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3362 4572 3362
Size: 1210 4260
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5470
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4081 136
Size: 107 122 71 631 605 2409 1525
Fragments arranged by size:
2409 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5465 5465
Size: 5470
BlpI GC'TnA_GC
Cuts at: 2345 2887 2345
Size: 542 4928
Bme1580I G_kGCm'C
Cuts at: 1790 2432 3531 4687 1790
Size: 642 1099 1156 2573
Fragments arranged by size:
2573 1156 1099 642
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5470
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2055 2375 3201
Size: 416 53 83 399 24 638 320 826
Cuts at: 3201 4159 442
Size: 958 1753
Fragments arranged by size:
1753 958 826 638 416 399 320 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5470
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2661 4130 1239
Size: 1422 1469 2579
Fragments arranged by size:
2579 1469 1422
Bpu10I CC'TnA_GC
Cuts at: 2052 2052
Size: 5470
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2683 3595 4460 4758 2683
Size: 912 865 298 3395
Fragments arranged by size:
3395 912 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2407 2701 2753 4133 882
Size: 617 908 294 52 1380 2219
Fragments arranged by size:
2219 1380 908 617 294 52
BsaAI yAC'GTr
Cuts at: 493 1204 3261 493
Size: 711 2057 2702
Fragments arranged by size:
2702 2057 711
BsaBI GATnn'nnATC
Cuts at: 5464 5464
Size: 5470
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2229 3277 3659 4294
Size: 53 83 186 936 696 1048 382 635
Cuts at: 4294 275
Size: 1451
Fragments arranged by size:
1451 1048 936 696 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2606 3903 4301 4575 4722 5133 2606
Size: 1297 398 274 147 411 2943
Fragments arranged by size:
2943 1297 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5245
Fragments arranged by size:
5245 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2476 1186
Size: 1290 4180
BseYI C'CCAG_C
Cuts at: 2199 2259 2442 4673 5169 2199
Size: 60 183 2231 496 2500
Fragments arranged by size:
2500 2231 496 183 60
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2510 2522 1163
Size: 573 774 12 4111
Fragments arranged by size:
4111 774 573 12
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2920 3681 4709 1540
Size: 275 186 919 761 1028 2301
Fragments arranged by size:
2301 1028 919 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2432 3531 3616 4687 2432
Size: 1099 85 1071 3215
Fragments arranged by size:
3215 1099 1071 85
BsmI GAATG_Cn'
Cuts at: 2981 3074 2981
Size: 93 5377
BsmBI CGTCTCn'nnnn_
Cuts at: 2239 2653 2239
Size: 414 5056
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2401 2432 2789 3531 3616 4687 1391
Size: 399 611 31 357 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 611 399 357 85 31
BspEI T'CCGG_A
Cuts at: 2606 5133 2606
Size: 2527 2943
BspHI T'CATG_A
Cuts at: 3359 5089 3359
Size: 1730 3740
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2126 2825 844
Size: 1282 699 3489
Fragments arranged by size:
3489 1282 699
BsrBI CCG'CTC
Cuts at: 1394 1823 3357 1394
Size: 429 1534 3507
Fragments arranged by size:
3507 1534 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2194 3965 4139 65
Size: 1183 946 1771 174 1396
Fragments arranged by size:
1771 1396 1183 946 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2438 2571 4114 4301 1700
Size: 110 628 133 1543 187 2869
Fragments arranged by size:
2869 1543 628 187 133 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5470
BssHII G'CGCG_C
Cuts at: 1925 2276 1925
Size: 351 5119
BssSI C'ACGA_G
Cuts at: 3530 4542 3530
Size: 1012 4458
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5470
BstBI TT'CG_AA
Cuts at: 4274 4274
Size: 5470
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5470
BstYI r'GATC_y
Cuts at: 1972 2121 2800 3167 3552 3569 5021 5107 5119
Size: 149 679 367 385 17 1452 86 12
Cuts at: 5119 5465 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 679 385 367 346 149 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2638 513
Size: 552 1573 3345
Fragments arranged by size:
3345 1573 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4731
Fragments arranged by size:
4731 683 56
BtsI GCAGTG_nn'
Cuts at: 2271 2522 2986 3780 3800 2271
Size: 251 464 794 20 3941
Fragments arranged by size:
3941 794 464 251 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5470
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5435
DraI TTT'AAA
Cuts at: 2774 2774
Size: 5470
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4477 809
Size: 507 3161 1802
Fragments arranged by size:
3161 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2436 2917 3806
Size: 54 1425 50 275 18 606 481 889
Cuts at: 3806 5435 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 606 481 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2917 1537
Size: 275 1105 4090
Fragments arranged by size:
4090 1105 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2347 3400 1353
Size: 994 1053 3423
Fragments arranged by size:
3423 1053 994
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2279 4057 4431 4577 387
Size: 1064 138 690 1778 374 146 1280
Fragments arranged by size:
1778 1280 1064 690 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2102 2661 3533 4130 4917 5185 1239
Size: 393 470 559 872 597 787 268 1524
Fragments arranged by size:
1524 872 787 597 559 470 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2716 2716
Size: 5470
EcoO109I rG'GnC_Cy
Cuts at: 2088 2612 2088
Size: 524 4946
EcoRI G'AATT_C
Cuts at: 2779 3157 2779
Size: 378 5092
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2608 2608
Size: 5470
FspI TGC'GCA
Cuts at: 3976 3976
Size: 5470
HaeII r_GCGC'y
Cuts at: 1678 2232 4617 1678
Size: 554 2385 2531
Fragments arranged by size:
2531 2385 554
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2113 2145 2176 2208 4111
Size: 32 135 32 541 32 31 32 1903
Cuts at: 4111 4143 4185 4217 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1903 541 135 42 32 32 32
32 32 32 31
HincII GTy'rAC
Cuts at: 669 1657 1938 2814 3060 669
Size: 988 281 876 246 3079
Fragments arranged by size:
3079 988 876 281 246
HindIII A'AGCT_T
Cuts at: 748 2734 748
Size: 1986 3484
HpaI GTT'AAC
Cuts at: 3060 3060
Size: 5470
KasI G'GCGC_C
Cuts at: 2228 2228
Size: 5470
KpnI G_GTAC'C
Cuts at: 2795 2795
Size: 5470
MfeI C'AATT_G
Cuts at: 3069 3069
Size: 5470
MluI A'CGCG_T
Cuts at: 5346 5346
Size: 5470
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4584 4768 1305
Size: 3279 184 2007
Fragments arranged by size:
3279 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3648
Fragments arranged by size:
3648 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3428 3787 3946 518
Size: 648 2262 359 159 2042
Fragments arranged by size:
2262 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2311 2344 2494 2886 3563 4711 4956 1606
Size: 705 33 150 392 677 1148 245 2120
Fragments arranged by size:
2120 1148 705 677 392 245 150 33
NaeI GCC'GGC
Cuts at: 2440 2440
Size: 5470
NarI GG'CG_CC
Cuts at: 2229 2229
Size: 5470
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4918
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5470
NgoMIV G'CCGG_C
Cuts at: 2438 2438
Size: 5470
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5470
NotI GC'GGCC_GC
Cuts at: 2917 2917
Size: 5470
NspI r_CATG'y
Cuts at: 1132 1229 2828 4373 1132
Size: 97 1599 1545 2229
Fragments arranged by size:
2229 1599 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2224 1348
Size: 876 4594
PciI A'CATG_T
Cuts at: 4369 4369
Size: 5470
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5470
PmeI GTTT'AAAC
Cuts at: 2774 2774
Size: 5470
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3539 3571 5052 5084 3539
Size: 32 1481 32 3925
Fragments arranged by size:
3925 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5470
PsiI TTA'TAA
Cuts at: 3040 3040
Size: 5470
PstI C_TGCA'G
Cuts at: 830 2822 830
Size: 1992 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5470
PvuII CAG'CTG
Cuts at: 2344 2494 2344
Size: 150 5320
SalI G'TCGA_C
Cuts at: 1936 2812 1936
Size: 876 4594
SbfI CC_TGCA'GG
Cuts at: 2822 2822
Size: 5470
ScaI AGT'ACT
Cuts at: 3718 5430 3718
Size: 1712 3758
SfcI C'TryA_G
Cuts at: 826 1045 2818 3953 4634 4825 826
Size: 219 1773 1135 681 191 1471
Fragments arranged by size:
1773 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2230 2230
Size: 5470
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5470
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5470
SmaI CCC'GGG
Cuts at: 1872 2419 2797 1872
Size: 547 378 4545
Fragments arranged by size:
4545 547 378
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2698 3574 4475 4737 820
Size: 197 926 755 876 901 262 1553
Fragments arranged by size:
1553 926 901 876 755 262 197
SnaBI TAC'GTA
Cuts at: 493 3261 493
Size: 2768 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5470
SphI G_CATG'C
Cuts at: 1132 2828 1132
Size: 1696 3774
SspI AAT'ATT
Cuts at: 5 52 3394 5
Size: 47 3342 2081
Fragments arranged by size:
3342 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5470
StyI C'CwwG_G
Cuts at: 513 1065 2909 513
Size: 552 1844 3074
Fragments arranged by size:
3074 1844 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2273 3497 3514 3667 3852 4297 325
Size: 1255 693 1224 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1224 693 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3716 5428 96
Size: 274 80 33 51 1443 1739 1712 138
Fragments arranged by size:
1739 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2501 2533 5430 5462 2501
Size: 32 2897 32 2509
Fragments arranged by size:
2897 2509 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5470
XbaI T'CTAG_A
Cuts at: 2806 2806
Size: 5470
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5470
XmaI C'CCGG_G
Cuts at: 1870 2417 2795 1870
Size: 547 378 4545
Fragments arranged by size:
4545 547 378
XmnI GAAnn'nnTTC
Cuts at: 3599 3599
Size: 5470
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3278 276
Size: 53 83 186 936 1744 2468
Fragments arranged by size:
2468 1744 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI
BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI
SexAI SfiI SrfI SwaI XcmI